• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1617-1621
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• 2004

The aim of this study was to investigate the apoptotic effect and its mechanism on Radix Paeoniae Alba Extract(RPAE) in DU145 human prostate cancer cell line. RPAE induced apoptosis in a dose-dependent manner in DU145 cells as confirmed by both discontinuous DNA fragmentation using Hoechst33342 staining and poly-(ADP-ribose) polymerase(PARP) cleavage, which are apoptotic signs. To clarify the mechanisms on RPAE-induced apoptosis, we examined the p50(NF-κB subunit), IκBα, PTEN and Par-4 protein expression using Western blotting. Treatment with RPAE resulted in the decrease of p50 expression by IκBα increase, which resulted in Par-4 increase and bcl-2 decrease in DU145 cells. These results suggest that apoptosis of DU145 cells by RPAE involved decreases of NF-κB activation and bcl-2 expression, increase of Par-4 protein expression.

• Herbal Formula Science
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• v.17 no.1
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• pp.45-59
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• 2009

To beef up natural immunity, we have used Bojoongikgitang which has been known to treat enervation in the oriental medicine. This study is analyzed out structure material and the chief virtue of a prescription through Literature Study on Bojoongikgitang and Clinical Application. And this study is investigated to make sure of the necessity and additional symptoms in using Bojoongikgitang. The results are as follows : 1. It is regarded that the structure materials of Bojoongikgitang consist of Astragali Radix one jeon(錢) five poon(分), Ginseng Radix, Atractylodis Macrocephalae Rhizoma, Glycyrrhizae Radix one jeon for each, Citri Pericarpium, Angelicae Gigantis Radix five poon for each, Cimicifugae Rhizoma, Bupleuri Radix three poon for each. 2. The necessity symptoms in using Bojoongikgitang are a pale complexion, drowsy eyes(目無精光), vigorless, lethargy, sluggish talk. 3. The fittest prescriptions prior to the necessity symptoms in using Bojoongikgitang are Bojoongikgitang added Paeoniae Radix Alba, Scutellariae Radix in fever, exterior heat, mild fever, Bojoongikgitang added Ephedrae Radix, Tritici Fructus Levis, Aconiti Iateralis Preparata Radix in spontaneous sweating, spontaneous sweating by yang deficiency, Bojoongikgitang added Paeoniae Radix Alba, Scutellariae Radix in feeling the pulse like a flood, largeness and weakness, scatter and largeness, flood and largeness for diagnosis respectively. Bojoongikgitang Entering the heart channel by culturing the blood prescription in vexation, vexation and anxiety, Soongihwajoongtang in headache, DossiBojoongikgitang in rigor, Bojoongikgitang annexed Saengmaecsan in thirst, Daninsamtang or Jojoongikgitang in asthma, asthma by congestion of the upwardness, Eeegongsan in light eating, eschewing food, losing one's appetite, Ikweeseungyangtang in deficiency failing to control blood and blood collapsey. 4. To treat a functional disease is superior to organic one in using Bojoongikgitang.

• Korean Journal of Pharmacognosy
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• v.30 no.4
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• pp.363-367
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• 1999

Palmultang(PMT) consists of Ginseng Radix Alba, Atractylodis Rhizoma Alba, Hoelen, Glycyrrhizae Radix, Rehmanniae Radix Preparata, Paeoniae Radix, Cnidii Rhizoma and Angelicae Gigantis Radix. PMT enhanced the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and inhibited the production of nitric oxide in murine peritoneal macrophage. PMT enhanced the production of ${\gamma}-interferon$, interleukin-2 and the cell viability in murine thymocyte, but did not affect the production of interleukin-4. These results indicate that PMT enhances the phagocytosis of macrophage via the stimulation of ${\gamma}-interferon$ production in $T_H1$ cells and the reduction of nitric oxide production in peritoneal macrophage.

• Journal of Haehwa Medicine
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• v.8 no.1
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• pp.837-851
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• 1999

In order to inverstigate interactive effects of Samultang(SMT) and SMT composition and the principle of prescription, I examined the effects of SMT and combination of three components of SMT, In C57BL/6 mouse with oral administration of SMT and cyclophosphamide(CTX) treatment, white blood cells, platelet were counted. Also, those were anti-anemia effect for rat which have hemolytic anemia induced by phenylhydrazine. The results were summerized as follows : 1. In vivo analysis of the effect of the SMT by orally administrated C57BL/6 mice with the SMT and was treated with cyclophosphamide (CTX). When the number of white blood cell. platelet was counted, there was ameliorative effects of leucopenia or thrombocytopenia as a protection to CTX. 2. It was acknowledged that SMT and its composition increased RBC count and hemlytic significantly in rat which have hemolytic anemia. 3. Among SMT composition Angelicae gigantis Radix and Cnidii Rhizoma solution and among the combination group Rehmanniae Radix and Cnidii Rhizoma combination and SMT solution showed significant effects for hemoglobin and weight of spleen in mouse which have hemolytic anemia. Thus it was acknowledged that Rehmanniae Radix and Cnidii Rhizoma combination had effect to improve hemoglobin and weight of spleen. From above these results it was acknowledged that SMT has effects that ameliorative effects of leucopenia or thrombocytopenia as a protection to CTX and anti-anemic effect for the rat which have hemolytic anemia induced by phenylhydrazine and these effects depend upon its combination of SMT composition. Especially it was acknowleged, though there are the slight difference according to combination, that Paeoniae Radix Alba combination had improving effect for physical change. So it is though that Paeoniae Radix Alba is main material for this kind of disease and continuous study is needed.

• Korean Journal of Pharmacognosy
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• v.29 no.4
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• pp.365-373
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• 1998

In order to estimate accurate quality of crude drugs in which pest was found during the storage, damaged 15 items were collected on current markets. We identified insect name by investigation of insect morphology, bug cluster, and morphology of damaged crude drugs. Three kind of pests were identified in the above 15 items as follows: A: Pyralis sp. (Pyralidae) was observed in damaged Platycodi Radix. B: Anthreus verbasci (Anobiidae) was found out in damaged Angelicae gigantis Radix, and Dioscoreae Rhizoma. C: Stegobium pamceum (Anobiidae) was discovered in the 12 crude drugs as follows: Puerariae Radix, Angelicae koreanae Radix, Angehcae tenuissimae Radix, Codonopsis Radix, Ledebouriellae Radix, Ginseng Radix alba, Angelicae dahuricae Radix, Belamcandae Rhizoma, Paeoniae Radix, Cnidii Rhizoma, Alismatis Rhizoma, and Fritillariae Bulbus.

• KSBB Journal
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• v.18 no.2
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• pp.90-93
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• 2003

Six heavy metals (lead, copper, cadmium, chromium, mercury and arsenic) were analyzed in 9 oriental medical materials(Paeoniae Radix Alba, Zizyphi Fructus, Cnidii Rhizoma, Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Astragali Radix, Glycyrrhizae Radix, Cinnamomi Cortex Spissus, and Zingiberis Rhizoma Crudus) by inductively coupled plasma atomic emission spectrometry (ICP-AES). The heavy metal contents of Zingberis Rhizoma Crudus and Angelicae Gigantis Radix were 44.0 and 37.3 mg/kg, respectively, which were 1.47 and 1.24 times higher than the guideline set by the Korean Food and Drug Administration (KFDA). Washing with deionized water lowered the heavy metal contents by 20-38%, and reduced levels to below the guidelines set by KFDA.

• Journal of the Korean Institute of Oriental Medical Informatics
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• v.11 no.1
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• pp.26-47
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• 2005

In this dissertation, I will focus on the channel entry, the effect, and the treatment throughout books of oriental medicine from ancient to modern in order to classify the medicines of the liver as main or supplementary organ. The results are as follows: 1. The kinds of the medicines of working of the liver(本臟) were 29, which were Amydae carapax(鱉甲), Chrysanthemi Flos(菊花), Cassiae Torae Semen(草決明), Plantaginis Semen(車前子), Scirpi Seu Sparganii(三稜), Ulmi Pasta Semen(蕪荑), Cinnamomi Cortex(肉桂), Fraxini Cortex(秦皮), Chaenomelis Fructus(木果), Corii Asini Colla(阿膠), Chuanxiong Rhizoma(川芎), Adenophorae Radix(沙蔘), Coicis Semen(薏苡仁), Acanthopanacis Cortex(五加皮), Zizyphi Spinosae Semen(酸棗仁), Picrorrhizae Rhizoma(胡黃連), Gentianae Radix(草龍膽), Citri Reticulatae Virdie Pericarpium(靑皮), Paeoniae Alba Radix(白芍藥), Paeoniae Rubra Radix(赤芍藥), Bupleuri Radix(柴胡), Peucedani Radix(前胡), Naturalis Indigo(靑黛), Citrus unshiu(橘葉), Rhinocerotis Cornu(犀角), Aucklandiae Radix(木香), Polygonati Odorati Rhizoma, Farfarae Flos(款冬花), Evodiae Fructus(吳茱萸), Citri Reticulatae Pericarpium(陳皮) . 2. The kinds of the medicines of working of other viscera(他臟) were 7, which were Astragaliadix(黃耆), Ginseng Radix(人蔘), pinelliae Rhizoma(半夏), Myristicae Semen, Euryales Semen, Arecae Semen, Piperis Longi Fructus. 3. Medicines, effected on the heart functioned through any other viscera are as follows: Arecae Semen works to treat Spleen Gi Entering the Liver(脾氣入肝), Piperis Longi Fructus, pinelliae Rhizoma(半夏), Euryales Semen and Myristicae Semen operate to treat Spleen Cold Entering the Liver(脾冷入肝), Astragali Radix(黃耆) and Ginseng Radix(人蔘) work to treat Spleen Vacuity Entering the Liver(脾虛入肝). In the study of concerning the medicines effected on the liver, It is considered that it dedicated to development of the medicines related to the disease of the liver and making efficient use of the medicines.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.561-570
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• 2004

To screen the effective materials for hair loss treatment, the Gamissanghwa-tang extracts were tested. As a result we found that the Gamissanghwa-tang extracts have the hair growth promoting effect. After topical application of each test materials to the back of CS7BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. In the experiments of 5α-reductase type II inhibition assay, Radix Paeoniae Alba, Semen Cuscutae showed effective potential to inhibit the activity of 5α-reductase type II. And hair growth index of the Gamissanghwa-tang extracts ranked as 1.2, especially the hair growth index of Fructus Rubi is highest as 1.8. But there were no plant extracts which have effect on the DNA proliferation of hair dermal papilla cell measured by [³H]thymidine incorporation, the expression of growth factors such as IGF-I, KGF, HGF estimated by RT-PCR and protein synthesis of vibrissae hair follicle measured by [/sup 35/S] cysteine incorporation. Cortex Cinnamomi showed anti-bacterial effect on P. ovale, Radix Paeoniae Alba has the highest radical scavening activity and Radix Glycyrrhizae has the highest effects of NO synthesis. These results suggest that Gamissanghwa-tang can be used as a potent treatment agent for helping hair growth stimulation.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.663-665
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• 2003

For the screening of anti-inflammation and antioxidative activities, ethanolic extract of 40 species of traditional herbal medicines were examined in vitro their hyaluronidase inhibitory effect and radical scavenging activity. Astragali Radix, Eucommia Cortex, Schizandrae Fructus, Scutellaria Radix and Moutan Radicis Coritex showed more than 50% hyaluronidase inhibitory effects. Moutan Radicis Cortex, Paeoniae Radix Alba, Plantaginis Semen and Sorbus commixta Hedl. showed more than 90% in electron donating activity. The scavenging effects of ethanolic extracts on hydroxyl radical showed significantly activity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.3
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• pp.367-372
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• 2010

The purpose of this study is to make a basis of in vivo or clinical study about alopecia. We investigated the prescriptions of alopecia by researching oriental medicine literature. We found the prescriptions used for alopecia. And we surveyed the frequency and classification of the herbs used for alopecia. The results of this study were recorded as follows. The most frequently used single herbs in the prescriptions were Angelicae Gigantis Radix(當歸), Glycyrrhizae Radix(甘草), Poria(茯笭), Paeoniae Radix Alba(白芍藥). Herbs specifically frequently used in the prescriptions of alopecia were as follows. Polygoni Multiflori Radix(何首烏), Rehmanniae Radix(生地黃), Cuscutae Semen(菟絲子), Bupleuri Radix(柴胡), Scutellariae Radix(黃芩), Viticis Fructus(蔓荊子), Aconiti Iateralis Preparata Radix(附子). Herbs frequently used were classified by property. The result was as follows. (()-The number of herbs) 補益藥(32), 解表藥(18), 淸熱藥(15) 收澀藥(9), 活血祛瘀藥(8), 利水滲濕藥(8).