[KSCI] Korea Science Citation Index Service

Study on the Effect of Gamissanghwa-tang and each Medicinal Plant Extract for the Hair Growth of the Mice using In vivo and In vitro Test

Yun Jeong Hun (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University)
Kim Nam Kwen (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University)
Lim Kyu Sang (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University)
Roh Seok Seon (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Daejeon University)
Hwang Chung Yeon (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.18, no.2, 2004 , pp. 561-570 More about this Journal

Abstract

To screen the effective materials for hair loss treatment, the Gamissanghwa-tang extracts were tested. As a result we found that the Gamissanghwa-tang extracts have the hair growth promoting effect. After topical application of each test materials to the back of CS7BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. In the experiments of 5α-reductase type II inhibition assay, Radix Paeoniae Alba, Semen Cuscutae showed effective potential to inhibit the activity of 5α-reductase type II. And hair growth index of the Gamissanghwa-tang extracts ranked as 1.2, especially the hair growth index of Fructus Rubi is highest as 1.8. But there were no plant extracts which have effect on the DNA proliferation of hair dermal papilla cell measured by [³H]thymidine incorporation, the expression of growth factors such as IGF-I, KGF, HGF estimated by RT-PCR and protein synthesis of vibrissae hair follicle measured by [/sup 35/S] cysteine incorporation. Cortex Cinnamomi showed anti-bacterial effect on P. ovale, Radix Paeoniae Alba has the highest radical scavening activity and Radix Glycyrrhizae has the highest effects of NO synthesis. These results suggest that Gamissanghwa-tang can be used as a potent treatment agent for helping hair growth stimulation.

Keywords

Gamissanghwa-tang(加味雙和湯); hair loss; hair growth stimulation;

Citations & Related Records

Reference

1	Androgen and human hair growth / [ Randall, V. A. ] / Clin. Endoclinol. DOI ScienceOn
2	Single-step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction / [ Chomczynski P.;Sacchi N. ] / Anal. Biochem.
3	/ [ 이건겁;김승곤;김신무;김영권;오흥백;정경석;정태화 ] / 진단미생물학
4	식용식물의 항산화효과 검색과 산초의 항산화성분 / [ 문숙임;류홍수;이희령;최재수 ] / 한국영양식량학회지
5	Effects of Ginkgo biloba extract and quercetin on lipopolysaccharide-induced release of nitric oxide / [ Wadsworth TL;Koop DR ] / Chem. Biol. Interact. DOI ScienceOn
6	Flurbiprofen enantiomers inhibit inducible nitric oxide synthase expression in RAW 264.7 macrophages / [ Hinz B;Brune K;Rau T;Pahl A. ] / Pharm.Res. DOI ScienceOn
7	Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability / [ Denizot F;Lang R. ] / Immunol.Methods DOI ScienceOn
8	Tolman, R. L., Raetz, C. R. H. and Ellsworth, K.: Identification and selective inhibition of an isozyme of steroid 5 -reductase in human scalp / [ Harris, G.;Azzolina, B.;Baginsky, W.;Cimis, G.;Rasmusson G. H. ] / Proc.Natl.Acad.Sci. DOI ScienceOn
9	New hair growth drugs and their nonclinical evaluation methods / [ Otomo S. ] / 香粧會誌
10	Researches on new growth promoters / [ Nishizawa, H. ] / 香粧會誌
11	The effect of Sophora root extract on the anagen elongation and isolation of active compound / [ Takahashi, T.;Hamada C.;Ishino A.;Kobayashi, K.;Kimura, T. and Tajima M ] / Third International Meeting of Hair Research Society
12	Effect of leaves of Ginkgo biloba on hair regrowth in C3H strain mouse / [ Kobayashi N.;Suzuki R.;Koide C.;Suzuki T.;Matsuda H and Kubo M ] / 藥學雜誌
13	/ [ 徐春甫 ] / 古今醫統大全(八)
14	/ [ 裵元植 ] / 最新漢方臨床學
15	/ [ 許浚 ] / 東醫寶鑑
16	Finasteride: a review of its use in male pattern hair loss / [ McClellan KJ;Markham A ] / Drugs DOI ScienceOn
17	/ [ 신민교 ] / 臨床本草學
18	탈모증에 대한 상백피 복합물의 모발성장 촉진 효과 / [ 윤성중(외3인) ] / 한국잠사학회지
19	고삼추출물이 모발성장촉진 및 면포 억제에 미치는 영향 / [ 노현찬;노석선 ] / 대한피부과학회지
20	5종의 한약재추출물이 탈모방지와 모발성장촉진에 미치는 실험적 연구 / [ 신연상 ] / 대전대학교 박사학위논문
21	The secret life of the hair follicle / [ Hardy, M. H. ] / Trends Genet. DOI
22	The growth of vibrissae dermal papilla cells in vitro / [ Jahoda, C. and Oliver, R. F. ] / Br. J. Dermatol.. DOI ScienceOn
23	The in vitro roperties of dermal papilla cell lines established from human hair follicles / [ Messenger, A. G.;Jennifer H. and Bleehen, S. S. ] / Br. J. Dermatol. DOI ScienceOn
24	human hair growth in vitro / [ Philpott, M. P.;Green, M. R. and Kealey, T. ] / J. Cell Sci.
25	FGF5 as a regulator of the hair growth cycle: evidence from targeted and spontaneous mutations / [ Hebert, J.M.;Rosenquist, T.;Gotz, J. and Martin, G.R. ] / Cell DOI ScienceOn
26	De Novo hair follicle morphogenesis and hair tumors in mice expressing a truncated beta-catenin in skin / [ Gat, U.;DasGupta, R.;Degenstein, L. and Fuchs, E. ] / Cell DOI ScienceOn
27	Essential role for Sonic hedgehog during hair follicle morphogenesis. / [ Chiang, C.;Swan, R.Z.;Grachtchouk, M.;Bolinger, M.;Litingtung,Y.;Robertson, E.K.;Cooper, M.K.;Gaffield, W.;Westphal, H.;Beachy, P. A. and Dlugosz, A. A. ] / Dev. Biol. DOI ScienceOn
28	Androgen and human hair growth / [ Randall, V. A. ] / Clin.Endoclinol. DOI ScienceOn
29	Characterization of Wnt gene expression in developingand postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis / [ Reddy, S.;Andl, T.;Bagasra, A.;Lu, M. M.;Epstein, D. J.;Morrisey, E. E. and Millar, S. E. ] / Mech. Dev DOI
30	Controls of hair follicle cycling / [ Stenn, K. S. and Paus, R. ] / Physiol. Rev. DOI
31	Regulation mechanisms of hair growth / [ Ogawa H. and Hattori M. ] / Current Problems in Dermatology
32	The culture of dermal papilla cells from human hair follicles / [ Messenger, A. G. ] / Br. J. Dermatol. DOI ScienceOn
33	Vibrissae dermal papilla cell aggregative behavior in vivo and in vitro / [ Jahoda, C.A.B. and Oliver R. ] / J.Embryol.Exp.Morph.
34	Development studies of cuticle drugs from natural resources. I. Effects of crude drug extracts on hair growth in mice / [ Kubo M.;Matsuda H.;Fukui M. and Nakai Y ] / 藥學雜誌
35	넙취 정유의 murine macrophage Raw 264.7 세포에서의 in vito 항암효과 / [ 김륭규;신경민;천상국;지사영;서성훈;박희준;최종원;이경태 ] / 약학회지
36	발암과정에 있어서 Cyclooxigenase-2의 역할 및 그 저해를 통한 화학 암예방 / [ 서영준 ] / 분자세포생물학뉴스
37	Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophage by two -carboline alkaloids extracted from Melia azedarach / [ Lee, B.G.;Kim, S.H.;Zee, O.P.;Lee, K.R.;Lee, H.Y.;Han J.W. and Lee, H.W. ] / European J. Pharmacol. DOI ScienceOn
38	Suppression by a sesquiterpene lactone from Carpesium divaricantum of inducible nitric oxide synthase by inhibiting nuclear factor-<TEX>$\kappa$</TEX>B activation Biochem / [ Kim, E.J.;Jin, H.K.;Kim .K.;Lee H.Y.;Lee, S.Y.;Lee, K.R.;Zee O.P.;Han, J.W. and Lee H.W. ] / Pharmarol
39	/ [ 素問 ] / 上古天眞論
40	/ [ 靈樞經橋釋 ] / 경맥편
41	/ [ 吳謙 ] / 醫宗金鑑
42	/ [ 葛洪 ] / 後備急方
43	/ [ 巢元方 ] / 諸病源候論校釋上冊
44	/ [ 孫思邈 ] / 備急千金要方
45	/ [ 祁坤 ] / 外科大成
46	/ [ 上海中醫學院 ] / 中醫外科學
47	普濟方券四十七 / [ 주숙 ] / 中國醫學大系16권
48	/ [ 劉河間 ] / 劉河間傷寒六書
49	/ [ 許浚 ] / 東醫寶鑑
50	/ [ 孟華燮 ] / 方藥指針
51	/ [ 黃度淵 ] / 醫宗損益
52	In vivo와 In vitro 평가 모델을 이용한 한약재추출물의 모발성장 및 촉진에 미치는 실험적 연구 / [ 강학천 ] / 원광대학교 박사학위논문
53	Male hormone stimulation is a prerequisite and an incitant in common baldness / [ Hamilton, J.B. ] / Am J Anat DOI
54	Steroid 5 -reductase : two genes /two enzymes / [ Russel D.W.; Wilson, J.D. ] / Annu.Rev..Biochem.
55	/ [ 素問 ] / 五臟生成篇

KSCI

Study on the Effect of Gamissanghwa-tang and each Medicinal Plant Extract for the Hair Growth of the Mice using In vivo and In vitro Test In vivo와 In vitro 실험에서 가미쌍화탕 및 구성한약재가 마우스의 모발 성장에 미치는 실험적 연구

Study on the Effect of Gamissanghwa-tang and each Medicinal Plant Extract for the Hair Growth of the Mice using In vivo and In vitro Test