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Study on Apoptosis-Inducing Effects and Mechanism of Radix Paeoniae Alba Extract in DU145 Human Prostate Cancer Cell  

Kwon Kang Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kim Eun Kyung (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kim Kyoung Jong (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kang Gil Seong (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kim Young Sun (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kim In Kyu (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kim In Seob (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kim In Soo (Department of Pathology, College of Oriental Medicine, Dongguk University)
Lee Su Kyung (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Seo Eun A (Department of Food and Nutrition, School of Human Envioronmental Science, Wonkwang University)
Ryu Do Gon (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1617-1621 More about this Journal
Abstract
The aim of this study was to investigate the apoptotic effect and its mechanism on Radix Paeoniae Alba Extract(RPAE) in DU145 human prostate cancer cell line. RPAE induced apoptosis in a dose-dependent manner in DU145 cells as confirmed by both discontinuous DNA fragmentation using Hoechst33342 staining and poly-(ADP-ribose) polymerase(PARP) cleavage, which are apoptotic signs. To clarify the mechanisms on RPAE-induced apoptosis, we examined the p50(NF-κB subunit), IκBα, PTEN and Par-4 protein expression using Western blotting. Treatment with RPAE resulted in the decrease of p50 expression by IκBα increase, which resulted in Par-4 increase and bcl-2 decrease in DU145 cells. These results suggest that apoptosis of DU145 cells by RPAE involved decreases of NF-κB activation and bcl-2 expression, increase of Par-4 protein expression.
Keywords
Radix Paeoniae Alba; DU145 human prostate cancer cell; apoptosis; NF-κB; Par-4; bcl-2;
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  • Reference
1 Cancer statistics /
[ Greenlee RT(et al.) ] / CA Cancer J Clin   ScienceOn
2 An assessment of radical prostatectomy. Time trends, geographic variation, and outcomes /
[ Lu-Yao GL(et al.) ] / The Prostate Patient Outcomes Research Team
3 Cancer control and quality of life following anatomical radical retropubic prostatectomy: results at 10 years /
[ Walsh PC;Partin AW;Epstein JI ] / J Urol
4 Androgen regulation of programmed death of normal and malignant prostatic cells /
[ Isaacs JT(et al.) ] / J Androl
5 Programmed cell death(apoptosis) and response to anti-cancer drugs /
[ Smets LA ] / Anticancer Drugs.
6 Review article: manipulation of cell death--the development of novel strategies for the treatment of gastro- intestinal disease /
[ Watson AJ ] / Aliment Pharmacol Ther.   ScienceOn
7 Novel therapeutic strategies to selectively kill cancer cells /
[ Panchal RG ] / Biochem Pharmacol.   ScienceOn
8 Inhibition of cancer cell proliferation and prostaglandin E2 synthesis by Scutellaria baicalensis /
[ Zhang DY(et al.) ] / Cancer Res
9 Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractory prostate carcinoma cells: mechanistic studies /
[ Hsieh TC(et al.) ] / Int J Oncol
10 /
[ 申佶求 ] / 申氏本草學
11 /
[ 辛民敎 ] / 原色臨床本草學
12 /
[ 許浚 ] / 東醫寶鑑
13 /
[ 李時珍 ] / 本草綱目(上)
14 /
[ 人民衛生出版社(編) ] / 原色中國本草圖鑑
15 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding /
[ Bradford MM ] / Anal Biochem.   ScienceOn
16 Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE /
[ Lazebnik YA ] / Nature
17 Biology and treatment of gliomas /
[ Janus TJ;Kyritsis AP;Forman AD;Levin VA ] / Ann Oncol
18 Clinical cure of glioblastoma-two case reports /
[ Yoshida T;Kawano N ] / Neurol Med Chir (Tokyo)
19 Caspase-dependent cleavage of signaling proteins during apoptosis: a turn-off mechanism for anti-apoptotic signals /
[ Widmann C;Gibson S ] / J.Biol.Chem   ScienceOn
20 Mice lacking ADPRT and poly (ADP-ribosyl)ation develop normally but are susceptable to skin disease /
[ Wang ZQ ] / Genes Dev.   ScienceOn
21 Therapy for patients with high grade astrocytoma using intraarterial chemotherapy and radiation therapy /
[ Madajewicz S;Chowhan N ] / Cancer
22 Function and activation of NF-kB in the immune system /
[ Baeuerle PA;Henkel T ] / Annual Review of Immunology
23 Par-4 drives trafficking and activation of Fas and Fasl to induce prostate cancer cell apoptosis and tumor regression /
[ Chakraborty M(et al.) ] / Cancer Res
24 Increased cardiomyocyte apoptosis and changes in proapoptotic and antiapoptotic genes bax and bcl-2 during left ventricular adaptations to chronic pressure overload in the rat /
[ Condorelli G;Morisco C;Stassi G;Notte A;Farina F;Sgaramella G;de Rienzo A;Roncarati R;Trimarco B;Lembo G ] / Circulation   ScienceOn
25 Cell death: the significance of apoptosis /
[ Wyllie AH;Kerr JF ] / Int Rev Cytol
26 The proto-oncogene Bcl-2 and its role in regulating apoptosis /
[ Kroemer G ] / Nature Medicine.   ScienceOn
27 Bcl-2 proteins : regulators of apoptosis or of mitochondrial homeostasis? /
[ Vander Heiden MG;Thompson CB ] / Nature Cell Biology.
28 Bax. The pro-apoptotic Bcl-2 family member, Bax. /
[ Brady HJ;Gil-Gomez G ] / International Journal of Biochemical Cell Biology   ScienceOn
29 Constitutive NF-kappa B activation, enhanced granulopoiesis, and neonatal lethality in I kappa B alpha-deficient mice /
[ Beg AA(et al.) ] / Genes Dev   ScienceOn