• Mycobiology
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• 제31권4호
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• pp.214-220
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• 2003

The fruiting body of Paecilomyces fumosoroseus was collected at Mt. Mani, Ganghwa Island, Korea in September, 2001. This study was carried out to obtain the basic informations for the mycelial growth and fruiting body production of P. fumosoroseus in artificial media. The optimal conditions for the mycelial growth were obtained at $25^{\circ}C$ and in the range of pH $6{\sim}9$, respectively. P. fumosoroseus showed the favorable growth on Hamada medium. The carbon and nitrogen source favorable for mycelial growth were dextrin and histidine, respectively. Optimum C/N ratio suitable for optimal growth of P. fumosoroseus was observed on the culture media adjusted to the ratio of 40:1. The mycelial growth of P. fumosoroseus was optimal on corn meal agar supplemented with 30% of silkworm pupae. The most favorable fruiting body formation of P. fumosoroseus was obtained in the medium containing unpolished rice supplemented with 20%(w/w) silk worm pupae at $25^{\circ}C$ under 100 lux.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• 한국잠사곤충학회지
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• 제42권1호
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• pp.28-30
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• 2000

Red muscardine (P. fumosoroseus), first observed on silkworm in Korea, was examined on its cultural characters, infection process, and symptoms. The fungus on PDA medium was grown to 44 mm in diameter in 14 days. Conidiaphore ws 1.45$\mu\textrm{m}$ in diameter, producing 4 to 6 phialides (5.9$\mu\textrm{m}$${\times}$1.8$\mu\textrm{m}$) in cluster. Elliptic conidia wre hyaline and 2.9${\times}$1.3$\mu\textrm{m}$ in size. Diseased silkworm showed typical symptoms of muscardine while becoming stiff. On 3 to 4 days after inoculation of P. fumosoroseus conidia upon the surface of silkworm, it was observed that the silkworm moved and ate less in rearing bed, and amorpnous dark spots of 1∼2 mm appeared on the surface o thorax, abdomen, spirace, and etc. Corrected mortality reached up to 97% at 9th day after inoculation.

• 농약과학회지
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• 제3권1호
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• pp.90-95
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• 1999

온실가루이(Trialeurodes vaporariorum)의 방제를 위하여, 우리나라 산림토양과 병사충으로부터 수종의 곤충병원성 곰팡이들을 분리하였다. 그 중 비교적 높은 독성을 보이는 세가지 균주를 선택하고 각각 Beauveria 속 SFB-582, Paecilomyces fumosoroseus SFP-198 및 Verticillium 속 SFV-1053이라 명명하였다. 특히 P. fumosoroseus SFP-198은 $10^{7}$ conidia/ml 농도에서 가장 높은 살충성($91.4{\pm}3.5%$)을 보였다. 실제 온실에서 온실가루이 유충의 성장정도에 따른 P. fumosoroseus SFP-198의 방제 효과는 기존에 보고된 균주인 V. lecanii F-903과 비슷하게 80-90%를 보였으며, 미생물 살충제로 제제화하기 위해 수화 보조제와 다른 첨가물을 가한 제제를 시설 하우스에 적용한 결과 수화 보조제로 Silwet L-77을 사용하였을 때 90% 이상의 방제 효과를 보여줌으로써 P. fumosoroseus SFP-198이 온실가루이 방제에 있어서 유용한 균주로 판단되어진다.

• Mycobiology
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• 제42권4호
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• pp.385-390
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• 2014

The beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is difficult to control using chemical insecticides because of the development of insecticide resistance. Several pest control agents are used to control the beet armyworm. Entomopathogenic fungi are one of the candidates for eco-friendly pest control instead of chemical control agents. In this study, among various entomopathogenic fungal strains isolated from soil two isolates were selected as high virulence pathogens against larva of beet armyworm. Control efficacy of fungal conidia was influenced by conidia concentration, temperature, and relative humidity (RH). The isolates Metarhizium anisopliae FT83 showed 100% cumulative mortality against second instar larvae of S. exigua 3 days after treatment at $1{\times}10^7$ conidia/mL and Paecilomyces fumosoroseus FG340 caused 100% mortality 6 days after treatment at $1{\times}10^4$ conidia/mL. Both M. anisopliae FT83 and P. fumosoroseus FG340 effectively controlled the moth at $20{\sim}30^{\circ}C$. M. anisopliae FT83 was significantly affected mortality by RH: mortality was 86.7% at 85% RH and 13.4% at 45% RH. P. fumosoroseus FG340 showed high mortality as 90% at 45% RH and 100% at 75% RH 6 days after conidia treatments. These results suggest that P. fumosoroseus FG340 and M. anisopliae FT83 have high potential to develop as a biocontrol agent against the beet armyworm.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2000년도 잠사과학 100주년 국제심포지엄 및 추계학술대회
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• pp.46-46
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• 2000

• International Journal of Industrial Entomology and Biomaterials
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• 제17권2호
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• pp.181-187
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• 2008

An entomopathogenic fungus, Paecilomyces fumosoroceus SFP-198 was isolated in Kyungbuk province, Korea and screened out for the control of Trialeurodes vaporariorum nymphs. It showed 72.5% efficacy against second instars of T. vaporariorum nymphs at 6 days after treatment in the laboratory. To select an active ingredient for the mass production and the formulation, SFP-198 culture products were compared based on their insecticidal activities against T. vaporariorum nymphs. Among them, conidia and blastospores showed much higher insecticidal activity than supernatants. Furthermore, SFP-198 conidia were more heat-resistant than blastospores, and also powder form of conidia was more stable than their suspension form. SFP-198 conidia showed high pathogenicity on not only T. vaporariorum but also Tetranychus urticae in the glasshouse. This result suggested that SFP-198 conidia can be used as a multi-targeting biological control agent against sucking agri- cultural pests, such as whiteflies and mites.

• 환경생물
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• 제22권3호
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• pp.400-404
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• 2004

유기합성적으로 제조된 지방족 폴리에스테르의 일종인 polycaprolactone (PCL)을 $27^\circ{C}$와 $37^\circ{C}$로 온도를 달리한 활성오니토양에 66일간 매립하여 토양미생물에 의해 분해된 PCL 필름의 표면변화를 관찰하고, PCL을 분해하는 토양진균과 토양세균을 분리, 동정하였다. PCL을 탄소원과 에너지원으로 이용하는 토양진균으로는 Paecilomyces fumosoroseus KH27, Penicillium digitatum KH28, Fusarium solani KH29와 Aspergillus sp. KH30, 토양세균으로는 Ochrobactrum anthropi KH3l이 분리되었다. $27^\circ{C}$에서는 P. digitatum KH28이 가장 높은 PCL 분해능(46%)을 보였고, $37^\circ{C}$에서는 O. anthropi KH31의 분해능(52%)이 가장 우수하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.229-234
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• 2004

Six entomopathogenic fungus isolates, Beauveria bassiana J57A, Nomuraea rileyi J125A, Paecilomyces farinosus J3A, Paecilomyces fumosoroseus J50A, Metarhizium anisopliae J88, Aspergilius sp. J64A, causing muscardine disease and aspergillosis in the silkworm, Bombyx mori were investigated for their cultural and morphological characteristics (on PDA culture media within 14 days at $24^{\circ}C$). The results showed that they differ each other from the features of cultural characteristics (colony elevation, colony color, colony growth rate) or morphological characteristics (conidiogenous cell structure, phialides, conidia size and shape). Among cultural characteristics, colony color is the easiest recognizable character between isolates. The morphological characteristics of each fungal isolate correspond to the descriptions of current system of classification.

• 한국잠사곤충학회지
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• 제44권1호
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• pp.32-36
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• 2002

7개도 22개 재배농가로 부터 수집한 시료는 총 529점이었으며, 수집균은 두 가지 유형으로 구분되며 생육장애에 의한 기형균은 23.2%, 오염균은 50.9%였다. 생육장애는 C-1, C-2, C-3 및 C-4의 4가지 유형이 있으며 이들 분생자병속은 20∼70 개가 발생되는데 회색, 순백색 또는 등황색을 떤다. 분생자병속 말단은 분지하지 않고 솜털형태로 뭉치거나, 발생 도중 쉽게 시들어 버린다. 분리된 오염균은 5종으로 Beauveria bassiana, Fusarium sp., Pacilomyces fumosoroseuse, Tricothecium roseum, Aspergiilus parasiticus이며, 이들은 분생자병속을 형성하지 않고 기주 표피에 각각 고유색상을 띠며 PDA 배지상에서 균사생장량은 14일간 43∼75 m 범위로 눈꽃동충하초균보다 빠른 생장을 보인다.