Abstract
Polycaprolactone (PCL), a synthetic aliphatic polyester, was buried in activated sludge soil for 66 days at $27^\circ{C}$ and $37^\circ{C}$. The morphology of the surface of PCL film degraded by soil microorganisms was observed. Soil microorganisms degrading PCL were isolated and identified. Soil fungi and soil bacteria utilizing PCL as carbon or energy source were identified as Paecilomyces fumosoroseus KH27, Penicillium digitatum KH28, Fusarium solani KH29, Aspergillus sp. KH30 and Ochrobactrum anthropi KH31, respectively. Biodegradation test of PCL by the isolated strains showed that, P. digitatum KH28 exhibited the most PCL degrading activity at $27^\circ{C}$. However, at $37^\circ{C}$ O. anthropi KH31 showed higher degrading activity than the other soil microorganisms tested.
유기합성적으로 제조된 지방족 폴리에스테르의 일종인 polycaprolactone (PCL)을 $27^\circ{C}$와 $37^\circ{C}$로 온도를 달리한 활성오니토양에 66일간 매립하여 토양미생물에 의해 분해된 PCL 필름의 표면변화를 관찰하고, PCL을 분해하는 토양진균과 토양세균을 분리, 동정하였다. PCL을 탄소원과 에너지원으로 이용하는 토양진균으로는 Paecilomyces fumosoroseus KH27, Penicillium digitatum KH28, Fusarium solani KH29와 Aspergillus sp. KH30, 토양세균으로는 Ochrobactrum anthropi KH3l이 분리되었다. $27^\circ{C}$에서는 P. digitatum KH28이 가장 높은 PCL 분해능(46%)을 보였고, $37^\circ{C}$에서는 O. anthropi KH31의 분해능(52%)이 가장 우수하였다.