• Progress in Superconductivity and Cryogenics
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• v.13 no.3
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• pp.31-35
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• 2011

This paper describes experimental study on GM-type pulse tube refrigerator with neon as a working fluid instead of helium. Neon gas has similar compression characteristics with helium gas because it is a monoatoimc gas. In experiments, a cooling performance test was performed with same compressor and pulse tube refrigerator for neon and helium as working gas. From experimental results, a PTR with neon show the improved cooling performance than a PTR with helium. Cooling performance and operating characteristics of a PTR were discussed and compared for two different working gas.

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.199-205
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• 2004

The nucleotide sequence of 8.6-kb EcoRI fragment containing sucrose phosphorylase gene isolated from Bifidobacterium longum SJ32 was determined. It was found that the fragment contained five open reading frames including the gene cluster for sucrose utilization such as a sucrose phosphorylase (ScrP), a sucrose transporter (ScrT), and a GalR-LacI-type transcriptional regulator (ScrR) identified by amino acid homology. Each gene showed over 94% amino acid homology among various B. longum strains. Genomic organization of the gene cluster is the same as those of other strains of B. longum but different from that of B. lactis. In spite of high homology of each gene among B. longum strains, the difference of flanking sequences of the gene cluster between strains SJ32 and NCC2705 insinuates the horizontal transfer of scrPTR between B. longum strains. The increase of sucrose phosphorylase activity in heterologous E. coli system by the co-expression of scrT with scrP against the single expression of scrP was measured. It seems to be the result of sucrose uptake increment by scrT in the host and is an indirect evidence that scrT is the gene for sucrose transport. The existence of multiple sucrose uptake systems in B. longum is supposed from the findings of several genes besides scrPTR involved in sucrose uptake in the genome of B. longum NCC2705.

• The Plant Pathology Journal
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• v.33 no.2
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• pp.133-139
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• 2017

Tan spot, caused by the fungus Pyrenophora tritici-repentis, is economically important foliar disease in Latvia, Lithuania, and Romania; however, race structure from Baltic States and Romania is not known. In this study, we performed genotypic and phenotypic race characterization of a large collection of P. tritici-repentis isolates from these countries to determine race structure and utilize this information for better disease management and breeding wheat for tan spot resistance. We characterized 231 single spore isolates from Latvia (n = 15), Lithuania (n = 107), and Romania (n = 109) for Ptr ToxA and Ptr ToxB genes using two genes specific primers. A subset (139) of 231 isolates were further characterized for their race structure by inoculating them individually on tan spot wheat differentials set. Majority (83%) of the 231 isolates amplified Ptr ToxA gene suggesting prevalence of race 1 and 2. Further, phenotypic characterization of 139 isolates also showed wide prevalence of races 1 (68%), 2 (8%), 3 (11%), and 4 (5%) were also identified from Baltic States as well as Romania. Eighteen of the isolates (13%) did not seem to be of any of the eight known races as they lacked Ptr ToxA gene but they behaved like either race 1 or race 2, suggesting possibility of novel toxins in these isolates as their virulence tools.

• Asian Journal of Atmospheric Environment
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• v.11 no.4
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• pp.300-312
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• 2017

To improve the understanding of secondary organic aerosol (SOA) formation from the photo-oxidation of anthropogenic and biogenic precursors at the regional background station on Baengnyeong Island, Korea, gas phase and aerosol chemistries were investigated using the Proton Transfer Reaction Time of Flight Mass Spectrometer (PTR-ToF-MS) and the Aerodyne High Resolution Time of Flight Aerosol Mass Spectrometer (HR-ToF-AMS), respectively. HR-ToF-AMS measured fine particles ($PM_1$; diameter of particle matter less than $1{\mu}m$) at a 6-minute time resolution from February to November 2012, while PTR-ToF-MS was deployed during an intensive period from September 21 to 29, 2012. The one-minute time-resolution and high mass resolution (up to $4000m{\Delta}m^{-1}$) data from the PTR-ToF-MS provided the basis for calculations of the concentrations of anthropogenic and biogenic volatile organic compounds (BVOCs) including oxygenated VOCs (OVOCs). The dominant BVOCs from the site are isoprene (0.23 ppb), dimethyl sulphide (DMS, 0.20 ppb), and monoterpenes (0.38 ppb). Toluene (0.45 ppb) and benzene (0.32 ppb) accounted for the majority of anthropogenic VOCs (AVOCs). OVOCs including acetone (3.98 ppb), acetaldehyde (2.67 ppb), acetic acid (1.68 ppb), and formic acid (2.24 ppb) were measured. The OVOCs comprise approximately 75% of total measured VOCs, suggesting the occurrence of strong oxidation processes and/or long-range transported at the site. A strong photochemical aging and oxidation of the atmospheric pollutants were also observed in aerosol measured by HR-ToF-AMS, whereby a high $f_{44}:f_{43}$ value is shown for organic aerosols (OAs); however, relatively low $f_{44}:f_{43}$ values were observed when high concentrations of BVOCs and AVOCs were available, providing evidence of the formation of SOA from VOC precursors at the site. Overall, the results of this study revealed several different SOA formation mechanisms, and new particle formation and particle growth events were identified using the powerful tools scanning mobility particle sizer (SMPS), PTR-ToF-MS, and HR-ToF-AMS.

• Journal of the Korea Safety Management & Science
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• v.12 no.1
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• pp.199-205
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• 2010

The research proposes the three-factor random measurement models for estimating the precision about operator, part, tool, and various measurement environments. The combined model with crossed and nested factors is developed to analyze the approximate F test by degrees of freedom given by Satterthwaite and point estimation of precisions from expected mean square. The model developed in this paper can be extended to the three useful models according to the type of nested designs. The study also provides the three-step procedures to evaluate the measurement precisions using three indexes such as SNR(Signal-To-Noise Ratio), R&R TR(Reproducibility&Repeatability-To-Total Precision Ratio), and PTR(Precision-To-Tolerance Ratio), The procedures include the identification of resolution, the improvement of R&R reduction, and the evaluation of precision effect.

• Journal of the Korea Safety Management & Science
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• v.11 no.3
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• pp.209-216
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• 2009

A development in method of evaluating the measurement precisions using approximate F tests and variance components from expected mean square (EMS) is investigated. The research proposes three-factor mixed measurement models with the fixed and random factors. Unrestricted and unconstrained design work was rarely studied, while restricted and constrained designs have been significantly discussed. The unrestricted and unconstrained designs assume to be an independence of interaction. The proposed evaluation method about the measurement precisions can be extended to four-factor random measurement model or mixed measurement model. The study also presents the three evaluation indexes of precisions such as R&RTR (Reproducibility & Repeatability-To-Total Precision Ratio), PTR (Precision-To-Tolerance Ratio), and SNR (Signal-To-Noise Ratio). Numerical examples are proposed to evaluate the approximate F tests with Satterthwaite degrees of freedom and three indexes using the measurement precisions from EMS.

• Progress in Superconductivity and Cryogenics
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• v.12 no.4
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• pp.36-40
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• 2010

In this paper, a noble method for rapid cooldown of pulse tube refrigerator (PTR) was proposed and experimentally investigated. An orifice pulse tube refrigerator generates refrigeration effect by expansion PV work at the cold-end, and its amount is affected by the orifice valve opening. There exists the optimum valve opening for maximum cooling capacity and it varies as cold-end temperature. It is verified from simulation results using isothermal model that the optimum valve opening increases as the cold-end temperature increases. In the experiments, a single stage orifice pulse tube refrigerator is fabricated and tested. The fabricated PTR shows 97.5 K of no-load temperature and 10 W at 110 K of cooling capacity with the fixed orifice valve opening. From experiments, the initial cooldown curve with four cases of valve opening control scenario are obtained. And it is experimentally verified that the initial cooldown time can be reduced through the control of orifice valve opening.

• Progress in Superconductivity and Cryogenics
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• v.14 no.4
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• pp.50-53
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• 2012

This paper describes experimental study on GM-type pulse tube refrigerator (PTR). In a PTR, the pulse tube is only filled with working gas and there exists secondary flow due to a large temperature difference between cold-end and warm-end. The stability of secondary flow is affected by orientation of cold-head and thus, the cooling performance is deteriorated by gas mixing due to secondary flow. In this study, a single stage GM-type pulse tube refrigerator is fabricated and tested. The cooing performance of the fabricated PTR is measured as varying cold-head orientation angle and the results are used as reference data. Then, we divided interior space of pulse tube into three segments, and fixed the various size of screen mesh at interface of each segment to suppress the performance degradation due to secondary flow. For various configuration of pulse tube, no-load test and heat load test are carried out with the fixed experimental condition of charging pressure, operating frequency and orifice valve turns. From experimental results, the fine screen mesh shows the effective suppression of performance degradation for the large orientation angle, but the use of screen mesh cause the loss of cooling capacity rather than the case of no insertion into pulse tube. It should be compromised whether the use of screen mesh in consideration of the installation limitation of a GM-type pulse tube refrigerator.

• Journal of the Korean Society of Urban Environment
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• v.18 no.4
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• pp.503-509
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• 2018

In this study, a Proton-Transfer Reaction-Time-of-Flight Mass spectrometer (PTR-TOF-MS) was used for the continuous monitoring of Volatile Organic Compounds (VOCs) emitted from semiconductor workplace such as photolithography (PHOTO), flat panel display (FPD), organic light emitting diode (OLED), etching (WET) process. The averaged VOCs mixing ratio in the such workplace, PHOTO was 6.5 ppm, FPH was 6.4 ppm, WET was 2.0 ppm and OLED was 1.3 ppm, respectively. The abundance of VOCs in the workplace were methyl ethyl ketone (MEK) with 2.8 ppm (69%) and acetaldehyde with 0.5 ppm (13.2%). Depending on the semiconductor process characteristics, various VOCs have been observed in the workplace. The VOCs mixing ratio are lower than the workplace regulation standard (TWA), it is necessary to continuously monitor and effectively manage these VOCs.

• Proceedings of the Korean Nuclear Society Conference
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• 2005.05a
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• pp.645-646
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• 2005