• 제목/요약/키워드: PRINCE

검색결과 737건 처리시간 0.025초

Characterization of a Potential Probiotic Lactiplantibacillus plantarum LRCC5310 by Comparative Genomic Analysis and its Vitamin B6 Production Ability

  • Yunjeong Lee;Nattira Jaikwang;Seong keun Kim;Jiseon Jeong;Ampaitip Sukhoom;Jong-Hwa Kim;Wonyong Kim
    • Journal of Microbiology and Biotechnology
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    • 제33권5호
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    • pp.644-655
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    • 2023
  • Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain's probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B6 biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917T , L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5'-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B6 supplementation.

Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces

  • Ngamlak Foongsawat;Sirinthorn Sunthornthummas;Kwannan Nantavisai;Komwit Surachat;Achariya Rangsiruji;Siriruk Sarawaneeyaruk;Kedvadee Insian;Sirapan Sukontasing;Nuttika Suwannasai;Onanong Pringsulaka
    • 한국축산식품학회지
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    • 제43권4호
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    • pp.685-702
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    • 2023
  • Lactic acid bacteria (LAB) are commonly used as probiotics; however, not all LAB strains have the same beneficial effects. To successfully use LAB as probiotics in canines, LAB species should originate from the canine intestinal tract as they display host specificity. The objective of this study was to investigate the phenotypic and genomic traits of potential probiotic LAB isolated from canine fecal samples. Twenty LAB samples were evaluated for their potential probiotic characteristics including resistance to low pH, bile salts, hydrophobicity, auto-aggregation, co-aggregation, adhesion to epithelia or mucosa, and production of inhibitory compounds. Additionally, we evaluated their safety and other beneficial effects on canine health, such as DPPH free radical scavenging, and β-galactosidase. Four strains demonstrated potential probiotic characteristics and were selected: Enterococcus hirae Pom4, Limosilactobacillus fermentum Pom5, Pediococcus pentosaceus Chi8, and Ligilactobacillus animalis FB2. Safety evaluations showed that all strains lacked hemolytic activity, could not produce biogenic amines, and did not carry any pathogenic genes. In addition, L. fermentum Pom5 and P. pentosaceus Chi8 displayed susceptibility to all antibiotics and concordant with the absence of antibiotic resistance genes. Based on their phenotypic and genomic characteristics, L. fermentum Pom5 and P. pentosaceus Chi8 were identified as potential probiotic candidates for canines.

Effect of visco-Pasternak foundation on thermo-mechanical bending response of anisotropic thick laminated composite plates

  • Fatima Bounouara;Mohamed Sadoun;Mahmoud Mohamed Selim Saleh;Abdelbaki Chikh;Abdelmoumen Anis Bousahla;Abdelhakim Kaci;Fouad Bourada;Abdeldjebbar Tounsi;Abdelouahed Tounsi
    • Steel and Composite Structures
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    • 제47권6호
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    • pp.693-707
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    • 2023
  • This article investigates the static thermo-mechanical response of anisotropic thick laminated composite plates on Visco-Pasternak foundations under various thermal load conditions (linear, non-linear, and uniform) along the transverse direction (thickness) of the plate, while keeping the mechanical load constant. The governing equations, which represent the thermo-mechanical behavior of the composite plate, are derived from the principle of virtual displacements. Using Navier's type solution, these equations are solved for the composite plate with simply supported condition. The Visco-Pasternak foundation type is included by considering the impact of the damping on the classical foundation model, which is modeled by Winkler's linear modulus and Pasternak's shear modulus. The excellent accuracy of the present solution is confirmed by comparing the results with those available in the literature. The study investigates the impact of geometric ratios, thermal expansion coefficient ratio, damping coefficient and foundation parameters on the thermo-mechanical flexural response of the composite plate. Overall, this article provides insights into the behavior of composite plates on visco-Pasternak foundations and may be useful for designing and analyzing composite structures in practical applications.

Mechanical and thermal stability investigation of functionally graded plates resting on visco-Pasternak foundation

  • Samira Hassiba Tagrara;Mohamed Mehdi hamri;Mahmoud Mohamed Selim Saleh;Mofareh Hassan Ghazwani;Abdelbaki Chikh;Abdelmoumen Anis Bousahla;Abdelhakim Kaci;Fouad Bourada;Abdelouahed Tounsi
    • Steel and Composite Structures
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    • 제46권6호
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    • pp.839-856
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    • 2023
  • This work presents a simple four-unknown refined integral plate theory for mechanical and thermal buckling behaviors of functionally graded (FG) plates resting on Visco-Pasternak foundations. The proposed refined high order shear deformation theory has a new displacement field which includes indeterminate integral variables and contains only four unknowns in which any shear correction factor not used, with even less than the conventional theory of first shear strain (FSDT). Governing equations are deduced from the principle of minimum total potential energy and a Navier type analytical solution is adopted for simply supported FG plates. The Visco-Pasternak foundations is considered by adding the impact of damping to the usual foundation model which characterized by the linear Winkler's modulus and Pasternak's foundation modulus. The accuracy of the present model is demonstrated by comparing the computed results with those available in the literature. Some numerical results are presented to show the impact of material index, elastic foundation type, and damping coefficient of the foundation, on the mechanical and thermal buckling behaviors of FG plates.

Effect of perlite powder on properties of structural lightweight concrete with perlite aggregate

  • Yan, Gongxing;Al-Mulali, Mohammed Zuhear;Madadi, Amirhossein;Albaijan, Ibrahim;Ali, H. Elhosiny;Algarni, H.;Le, Binh Nguyen;Assilzadeh, Hamid
    • Structural Engineering and Mechanics
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    • 제84권3호
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    • pp.393-411
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    • 2022
  • A high-performance reactive powder concrete (RPC) has been readied alongside river sand, with 1.25 mm particle size when under the condition of 80C steam curing. As a heat and sound insulation, expanded perlite aggregate (EPA) provides economic advantages in building. Concrete containing EPA is examined in terms of cement types (CEM II 32.5R and CEM I 42.5R), doses (0, 2%, 4% and 6%) as well as replacement rates in this research study. The compressive and density of concrete were used in the testing. At the end of the 28-day period, destructive and nondestructive tests were performed on cube specimens of 150 mm150 mm150 mm. The concrete density is not decreased with the addition of more perlite (from 45 to 60 percent), since the enlarged perlite has a very low barrier to crushing. To get a homogenous and fluid concrete mix, longer mixing times for all the mix components are necessary due to the higher amount of perlite. As a result, it is not suggested to use greater volumes of this aggregate in RPC. In the presence of de-icing salt, the lightweight RPC exhibits excellent freeze-thaw resistance (mass is less than 0.2 kg/m2). The addition of perlite strengthens the aggregate-matrix contact, but there is no apparent ITZ. An increased compressive strength was seen in concretes containing expanded perlite powder and steel fibers with good performance.

Application of computer methods for the effects of nanoparticles on the frequency of the concrete beams experimentally and numerically

  • Chencheng Song;Junfeng Shi;Ibrahim Albaijan;H. Elhosiny Ali;Amir Behshad
    • Steel and Composite Structures
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    • 제48권1호
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    • pp.19-25
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    • 2023
  • Due to high application of concrete structures in construction industry, however, the quality improvement is essential. One of the new ways for this purpose is adding the nanoparticles to the concrete. In this work, vibration analysis of concrete beams reinforced by graphene oxide (GO) nanoparticles based on mathematical model has been investigated. For the accuracy of the presented model, the experimental study is done for comparing the compressive strength. Since the nanoparticles can not be solved in water without any specific process, at the first, GO nanoparticles should be dispersed in water by using shaker, magnetic striker, ultrasonic devices and finally mechanical mixer. For modelling of the strucuture, sinusoidal shear deformation beam theory (SSDBT) is utilized. Mori-Tanak model model is utilized for obtaining the effective properties of the beam including agglomeration influences. Utilizing the energy method and Hamilton's principal, the motion equations are calculated. The frequency of the concrete beam is obtanied by analytical method. Three samples with 0.02% GO nanoparticles are built and its compressive strength is compared which shows a good accuracy with maximum 1.29% difference with mathematical model and other papers. The aim of this work from the theoretical study is investigating the effects of nanoparticles volume percentage and agglomeration, length and thickness of the beam on the frequency of the structure. The results show that the with enhancing the GO nanoparticles, the frequency is increased. For example, with enhancing the volume percent of GO nanoparticles from zero to 0.08%, the compressive strength is increased 48.91%. and 46.83%, respectively for two cases of with and without agglomeration.

Machine learning techniques for reinforced concrete's tensile strength assessment under different wetting and drying cycles

  • Ibrahim Albaijan;Danial Fakhri;Adil Hussein Mohammed;Arsalan Mahmoodzadeh;Hawkar Hashim Ibrahim;Khaled Mohamed Elhadi;Shima Rashidi
    • Steel and Composite Structures
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    • 제49권3호
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    • pp.337-348
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    • 2023
  • Successive wetting and drying cycles of concrete due to weather changes can endanger the safety of engineering structures over time. Considering wetting and drying cycles in concrete tests can lead to a more correct and reliable design of engineering structures. This study aims to provide a model that can be used to estimate the resistance properties of concrete under different wetting and drying cycles. Complex sample preparation methods, the necessity for highly accurate and sensitive instruments, early sample failure, and brittle samples all contribute to the difficulty of measuring the strength of concrete in the laboratory. To address these problems, in this study, the potential ability of six machine learning techniques, including ANN, SVM, RF, KNN, XGBoost, and NB, to predict the concrete's tensile strength was investigated by applying 240 datasets obtained using the Brazilian test (80% for training and 20% for test). In conducting the test, the effect of additives such as glass and polypropylene, as well as the effect of wetting and drying cycles on the tensile strength of concrete, was investigated. Finally, the statistical analysis results revealed that the XGBoost model was the most robust one with R2 = 0.9155, mean absolute error (MAE) = 0.1080 Mpa, and variance accounted for (VAF) = 91.54% to predict the concrete tensile strength. This work's significance is that it allows civil engineers to accurately estimate the tensile strength of different types of concrete. In this way, the high time and cost required for the laboratory tests can be eliminated.

Resistance of Polystyrene-Impregnated Glued Laminated Lumbers after Exposure to Subterranean Termites in a Field

  • Dede HERMAWAN;Mahdi MUBAROK;Imam Busyra ABDILLAH;Yusuf Sudo HADI;Cossey YOSI;Aujchariya CHOTIKHUN;Rohmah PARI;Gustan PARI
    • Journal of the Korean Wood Science and Technology
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    • 제52권1호
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    • pp.70-86
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    • 2024
  • Termites are a serious threat to wood-based products in Indonesia. This study investigated the termite resistant property of glulam made from polystyrene-modified wood. Three tropical fast-growing wood species, namely mangium (Acacia mangium), manii (Maesopsis eminii), and rubberwood (Hevea brasiliensis), were prepared for flat-sawn laminae. After getting air-dried condition, the laminae were impregnated with polystyrene using potassium peroxydisulphate as a catalyst followed by polymerization at 80℃. Polystyrene-impregnated and control glued-laminated lumbers (glulams) were manufactured, and solid wood was provided. Three wood species and three wood products with six replicates were exposed in a field in Bogor, Indonesia, for four months, and before the tests, their density and moisture content were measured. At the end of the field tests, the weight loss and protection levels of each test sample were determined. A completely randomized factorial design was used for data analysis. The weight percentage gains for mangium, manii, and rubberwood were 22.30%, 18.22%, and 10.44%, respectively. The results showed that manii belonged to low-density wood, whereas the other two woods were medium-density wood, and the moisture content was the ambient moisture content, typical of the Bogor area. Regarding weight loss and protection level, mangium was the most durable against subterranean termite attacks, followed by rubberwood and manii. Among the wood products, the polystyrene-impregnated glulam presented the highest durability, followed by the control glulam and solid wood. Therefore, mangium and rubberwood polystyrene-impregnated glulams are recommended for future product development.

Rain Attenuation Prediction at Different Time Percentages for Ku, K, and Ka Bands Satellite Communication Systems over Nigeria

  • Orji Prince Orji;Obiegbuna Dominic Chukwuebuka;Okoro Eucharia Chidinma;Ugonabo Obiageli Josephine;Okezuonu Patrick Chinedu;Iyida Evaristus Uzochukwu;Ugwu Chukwuebuka Jude;Menteso Firew Meka;Ikechukwu Ugochukwu Chiemeka
    • Journal of Astronomy and Space Sciences
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    • 제41권1호
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    • pp.25-33
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    • 2024
  • This paper evaluates the influence of rainfall on propagated signal at different time exceedance percentages of an average year, over the climate zones of the country. Specifically, it demonstrates critical and non critical signal fade or signal outage time exceedance (0.001% to 1%) for Ku, K, and Ka-band systems in an average year. The study was carried out using meteorological data made available by the Nigerian Meteorological Agency (NiMet) over a period of 10 years (2009-2018). The four climate zones in the country were represented by five (5) locations; Maidugiri (warm desert climate), Sokoto (tropical dry climate), Port Harcourt (tropical monsoon climate), Abuja and Enugu (tropical savanna climate). The parameters were simulated into the International Telecommunications Union Recommended (ITU-R) models for rain attenuation over the tropics and results presented using MatLab and Origin Lab. Results of Ku band propagations showed that only locations in the tropical savanna and tropical monsoon climates experienced total signal outage for time percentage exceedance equal to or below 0.01% for both horizontal and vertical polarizations. At K band propagations, the five locations showed to have experienced signal outage at time exceedance equal to and below 0.01%, almost same was recorded for the Ka-band propagation. It was also observed that horizontal and vertical polarization of signal had slightly different rain attenuation values for the studied bands at the five locations, with horizontal polarization having higher values than vertical polarization.

Static analysis of nonlinear FG-CNT reinforced nano-composite beam resting on Winkler/Pasternak foundation

  • Mostefa Sekkak;Rachid Zerrouki;Mohamed Zidour;Abdelouahed Tounsi;Mohamed Bourada;Mahmoud M Selim;Hosam A. Saad
    • Advances in nano research
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    • 제16권5호
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    • pp.509-519
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    • 2024
  • In this study, the static analysis of carbon nanotube-reinforced composites (CNTRC) beams resting on a Winkler-Pasternak elastic foundation is presented. The developed theories account for higher-order variation of transverse shear strain through the depth of the beam and satisfy the stress-free boundary conditions on the top and bottom surfaces of the beam. To study the effect of carbon nanotubes distribution in functionally graded (FG-CNT), we introduce in the equation of CNT volume fraction a new exponent equation. The SWCNTs are assumed to be aligned and distributed in the polymeric matrix with different patterns of reinforcement. The rule of mixture is used to describe the material properties of the CNTRC beams. The governing equations were derived by employing Hamilton's principle. The models presented in this work are numerically provided to verify the accuracy of the present theory. The analytical solutions are presented, and the obtained results are compared with the existing solutions to verify the validity of the developed theories. Many parameters are investigated, such as the Pasternak shear modulus parameter, the Winkler modulus parameter, the volume fraction, and the order of the exponent in the volume fraction equation. New results obtained from bending and stresses are presented and discussed in detail. From the obtained results, it became clear the influence of the exponential CNTs distribution and Winkler-Pasternak model improved the mechanical properties of the CNTRC beams.