• 한국암석학회:학술대회논문집
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• 한국암석학회 2004년도 춘계학술대회
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• pp.32-34
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• 2004

• 한국과학교육학회지
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• 제43권3호
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• pp.209-223
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• 2023

이 연구는 초·중등 학생의 극지 소양 함양을 위해 개발된 극지 소양 교육 프로그램을 적용한 효과를 평가하고, 기후 변화 대응 교육을 위한 시사점을 도출하기 위해 수행되었다. Polar-ICE 단체가 정립한 7가지 극지 소양 원리를 중심으로 개발한 모듈식 프로그램 중 과학개념을 강조한 프로그램과 인문학과 사회학적 쟁점을 강조한 프로그램 등 두 과정에 적합한 세부 프로그램을 선정해서 구조화했다. 이들 두 가지 프로그램은 서울 소재 대학 과학교육과에서 주최한 토요 과학교실에서 초등학생과 중학생 26명에게 약 69시간에 걸쳐 적용되었다. 26명의 학생을 세 집단으로 나누어 두 집단에게는 각각 극지 소양을 위한 과학 교육 프로그램과 인문 사회학적 쟁점 교육 프로그램을 실시했고, 나머지 한 집단은 극지 소양과 관련 없는 일반 과학 교육을 적용하여 대조군이 되었다. 프로그램을 적용하기 전후에 세 집단의 학생들은 모두 동일하게 극지 소양 검사와 극지에 대한 연상 단어와 장면을 진술하는 설문에 응답했다. 검사 결과는 비모수검정 방법인 Wilcoxon의 대응쌍 부호순위로 나타내어 프로그램 적용 전후의 향상도를 비교했다. 연구 결과 인지적 측면에서 실험군과 대조군 모두 지식 측면에서 프로그램 적용 후 향상이 나타났으나 실험군이 대조군 보다 향상의 정도가 더 컸으며, 명시적으로 다룬 내용이나 소재에서 특히 분명한 차이를 나타냈다. 정의적 측면에서 프로그램 전후의 차이는 그다지 큰 차이를 나타내지 않았지만, 인문 사회학적 쟁점을 다루었던 집단은 통계적으로 유의미한 향상을 보였다. 극지 심상의 변화는 실험 집단인 두 집단이 대조 집단에 비해 단조로운 이미지에서 좀 더 다양한 이미지로 분산되는 경향이 보였다. 이상의 연구 결과를 바탕으로 극지 소양 원리 과학 교육 프로그램의 효과를 높이기 위해 유의할 점, 과학적 사고를 함양하고 지구계 교육에 도움이 되는 소재로 활용할 필요성, 극지에 대한 태도를 향상하기 위한 방안, 기후 위기 대응을 준비하는 학교 교육 과정과의 연계 등의 시사점을 제안했다.

• 한국산업보건학회지
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• 제6권2호
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• pp.209-221
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• 1996

The purpose of this study was to find a suitable cosolvent to $CS_2$ so that desorption efficiency can be improved for both polar and non-polar organic solvent mixtures collected on an activated charcoal tube. Cosolvents added to $CS_2$ include: DMF(N,N-dimethylformamide): $CS_2$ (v/v 1:99), DMF:$CS_2$(v/v 3:97), BC (butyl carbitol, 2-(2-butoxy ethoxy) ethanol):$CS_2$(v/v 1:99), and BC:$CS_2$(v/v 3:97)). The results obtained were as follows : 1. Comparing the desorption efficiency of $CS_2$ with those of $CS_2$ with 1, 3, 5 % DMF and 1, 3 % BC cosolvents for two different groups of charcoal tubes each containing 8 different polar and non-polar organic solvents with 3 different concentration levels, the desorption efficiencies of the cosolvent-added $CS_2$ increased significantly for all polar organic solvents regardless of concentration levels tested. For non-polar organic solvents, no noticeable improvement was detected except xylene and trichloroethylene. The desorption efficiency of xylene increased significantly while that of trichloroethylene increased significantly at the lowest concentration level tested. 2. Either 5 % DMF or 3 % BC was the most suitable cosolvent because the desorption efficiency for non-polar organic solvent mixtures was similar or slightly improved compared with that of $CS_2$, while those of for polar organic solvent mixtures were above 75 % except for cyclohexanone. 3. The smallest variations in desorption efficiency represented by the ratio calculated from the maximum to minimum desorption efficiency for all concentration levels tested were found when 3 % BC was used as a cosolvent. The above results indicate that the desorption efficiency of $CS_2$ particularly for polar organic solvent mixtures collected on a charcoal tube can be significantly improved by the use of cosolvents such as 5 % DMF or 3 % BC. A caution, however, is in order for selecting a cosolvent whenever the cosolvent itself is being used in the workplace or the impurities contained in the cosolvent may interfere with the analytical results. In addition, to improve desorption efficiencies for such organic solvents as cyclohexanone or ketones, it is recommended to use suitable collection and desorption media other than the traditional method of charcoal tube collection/$CS_2$ desorption.

• 한국산업보건학회지
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• 제5권1호
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• pp.104-118
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• 1995

This study was performed to evaluate factors affecting desorption of organic solvents collected on charcoal tube and to find out the optimum condition. Desorption efficiency for polar analytes was improved when several polar desorption solvents such as methanol, dimethylformamide(DMF), 2-(2-butoxyethoxy)ethanol were added to carbon disulfide($CS_2$). The best improvement was achieved when 10% dimethylformamide(DMF) in $CS_2$ was used as desorption solvent. During storage of polar analytes, recovery was greatly reduced. Especially, the recovery of cyclohexanone was decreased to 18.1 % after a month storage at $34^{\circ}C$. After two weeks storage, recovery of polar analytes was sharply decreased. Water adsorbed on charcoal interfered the recovery of polar analytes but didn't interfere that one of nonpolar solvent, toluene. When 10% DMF in $CS_2$ was used as desorption solvent, the effect of water on recovery was decreased, comparing with Desorption efficiency increased when analyte loading increased, and usage of 10% DMF in $CS_2$ decreased the loading effect. Increasing volume of desorption solvent was not effective to improve desorption efficiency of analytes when 10% DMF was used. Continuous shaking and sonication is not helpful to increase the desorption efficiency of analytes except cyclohexanone using 10% DMF. When silica gel used as adsorbent, methanol was better desorbent than dimethylsulfoxide. Analytes adsorbed on silica gel showed high recovery in low concentration and less affected by humidity. On the basis of this study, the following conclusions have been drawn. To improve the recovery of polar organic materials in air samples, it is necessary to analyze samples as soon as possible after they were collected. Otherwise, samples must be stored at low temperature. Using two components of desorption solvents, such as 10% DMF in $CS_2$, the effects of loading and humidity decreased for polar analytes such as methyl ethyl ketone and methyl isobutyl ketone. When work place has high humidity with low concentration of polar organic solvents, silica gel can be used as adsorbent, because it produces quantitative recovery for polar analytes at this condition. But it should be noted that high humidity makes breakthrough easy in silica gel samples.

• KSBB Journal
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• 제30권6호
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• pp.345-349
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• 2015

Antifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris icebinding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from $37^{\circ}C$ to $15^{\circ}C$ and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity ($1.53^{\circ}C$) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs.

• Ocean and Polar Research
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• 제36권4호
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• pp.319-326
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• 2014

Mineral dust in the polar snow plays an important role both in the climate system of the Earth and in global biogeochemical cycles. Analysis of the concentration and the particle size distribution of mineral dust has been carried out in the snow from the Antarctic surface snow and the Greenland snowpit. Among the various particle size determination techniques, a Multisizer 3 Coulter Counter in a class 100 clean bench counted all particles between 1.1 and $30.0{\mu}m$ with a $50{\mu}m$ aperture tube. The aperture tube size, the concentration of electrolytes and the accuracy of the particle size distribution were determined in this study. The number concentrations from the Antarctic surface snow were 81,843 particles $mL^{-1}$, but those from the Greenland snowpit were 10,666 particles $mL^{-1}$. In the volume distribution, the distributions of mineral dust in both the Antarctic surface snow and the Greenland snowpit showed lognormal distribution in the size interval 1.1 to $6.0{\mu}m$ with the mode, 3.562 and $3.836{\mu}m$, respectively. The analysis technique using a coulter counter for mineral dust could be used for reconstructing paleoclimates from polar ice cores.

• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1472-1482
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• 2017

Bacterial cytochrome P450 (CYP) steroid hydroxylases are effectively useful in the pharmaceutical industry for introducing hydroxyl groups to a wide range of steroids. We found a putative CYP steroid hydroxylase (BaCYP106A2) from the bacterium Bacillus sp. PAMC 23377 isolated from Kara Sea of the Arctic Ocean, showing 94% sequence similarity with BmCYP106A2 (Bacillus megaterium ATCC 13368). In this study, soluble BaCYP106A2 was overexpressed to evaluate its substrate-binding activity. The substrate affinity ($K_d$ value) to 4-androstenedione was $387{\pm}37{\mu}M$. Moreover, the crystal structure of BaCYP106A2 was determined at $2.7{\AA}$ resolution. Structural analysis suggested that the ${\alpha}8-{\alpha}9$ loop region of BaCYP106A2 is intrinsically mobile and might be important for initial ligand binding. The hydroxyl activity of BaCYP106A2 was identified using in vitro enzyme assays. Its activity was confirmed with two kinds of steroid substrates, 4-androstenedione and nandrolone, using chromatography and mass spectrometry methods. The main products were mono-hydroxylated compounds with high conversion yields. This is the second study on the structure of CYP106A steroid hydroxylases, and should contribute new insight into the interactions of bacterial CYP106A with steroid substrates, providing baseline data for studying the CYP106A steroid hydroxylase from the structural and enzymatic perspectives.

• 마이크로전자및패키징학회지
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• 제21권4호
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• pp.45-49
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• 2014

본 연구에서는 낮은 비용과 간단한 공정의 장점을 가지고 있는 저온수열합성법을 이용하여 r-plane (1-102) sapphire 기판 위에 non-polar a-plane ZnO 박막을 성장하였다. 일반적으로 nanorod 형태의 ZnO를 성장시키는 특성을 보이는 Hexamethylenetetramine (HMT)와 2D layer 형태의 ZnO를 성장특성을 보이는 것으로 알려진 sodium citrate, 두 가지 전구체를 동시에 첨가하여 성장 하였을 때 몰 농도의 변화에 따른 ZnO 성장 특성을 비교해 보았다. ZnO 구조체의 형태와 특성 변화에 대하여 field emission scanning electron microscope (FE-SEM), high resolution X-ray diffraction(HRXRD)을 이용하여 분석을 진행하였다. 결과적으로, 두 가지의 용액의 특정 몰 농도일 때 r-plane (1-102) sapphire 기판 위에서 non polar a-plane (11-20) ZnO 구조체가 성장 될 수 있음을 확인 하였다. 이는 첨가제 조건에 의하여 c축 성장을 억제시키고, 측면 성장을 촉진시키는 반응에 의한 것으로 생각된다.

• ALGAE
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• 제31권1호
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• pp.61-72
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• 2016

Arctic microalgae thrive and support primary production in extremely cold environment. Three Arctic green microalgal strains collected from freshwater near Dasan Station in Ny-Alesund, Svalbard, Arctic, were analyzed to evaluate the optimal growth conditions and contents of fatty acids. The optimal growth temperature for KNF0022, KNF0024, and KNF0032 was between 4 and 8℃. Among the three microalgal strains, KNF0032 showed the maximal cell number of 1.6 × 107 cells mL^-1 at 4℃. The contents of fatty acids in microalgae biomass of KNF0022, KNF0024, and KNF0032 cultured for 75 days were 37.34, 73.25, and 144.35 mg g^-1 dry cell weight, respectively. The common fatty acid methyl esters (FAMEs) analyzed from Arctic green microalgae consisted of palmitic acid methyl ester (C16:0), 5,8,11-heptadecatrienoic acid methyl ester (C17:3), oleic acid methyl ester (C18:1), linoleic acid methyl ester (C18:2), and α-linolenic acid methyl ester (C18:3). KNF0022 had high levels of heptadecanoic acid methyl ester (26.58%) and heptadecatrienoic acid methyl ester (22.17% of the total FAMEs). In KNF0024 and KNF0032, more than 72.09% of the total FAMEs consisted of mono- and polyunsaturated fatty acids. Oleic acid methyl ester from KNF0032 was detected at a high level of 20.13% of the FAMEs. Arctic freshwater microalgae are able to increase the levels of polyunsaturated fatty acids under a wide range of growth temperatures and can also be used to produce valuable industrial materials.

• 대한전자공학회논문지SP
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• 제47권1호
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• pp.44-52
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• 2010

본 논문에서는 기존 평행좌표를 이용하는 얼굴영상 대신 극 좌표계 변환을 이용한 얼굴 인식 방법을 제안한다. 이 방법은 얼굴의 중심부분의 한 점을 극으로 삼아 이 점을 기준으로 360도의 각 방향으로 일정 길이만큼 얼굴 영상을 샘플링하여 새로운 얼굴 영상을 제작하고 이를 바탕으로 기존의 특징 추출 방법들을 이용하여 얼굴 인식의 성능을 높인다. 극 좌표계의 특성상 극에 가까운 부분은 세밀하게 묘사되고 극에서 멀리 떨어질수록 영상의 정확도가 떨어진다. 일반적으로 얼굴 영상은 얼굴의 중심부에 가까운 영역에 눈, 코, 입 등의 주요 부위가 밀집되어 있다. 따라서 이러한 극 좌표계를 얼굴영상에 적용한다면 같은 화소를 이용하는 기존 평행좌표를 사용할 때보다 눈, 코, 입 등 주요 부위를 보다 세밀하게 표현할 수 있다는 장점을 갖는다. 제안된 방법을 Yale데이터와 FRGC데이터에 적용한 후 기존의 특징 추출 방법인 LDA와 NLDA를 이용하여 얼굴인식을 수행한 결과 평행좌표에 기반한 원 영상을 그대로 사용했을 때 보다 인식률이 향상됨을 확인할 수 있었다.