Pilot-scale Production of the Antifreeze Protein from Antarctic Bacterium Flavobacterium frigoris PS1 by Recombinant Escherichia coli with a Cold Shock Induction System

Antifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris icebinding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from $37^{\circ}C$ to $15^{\circ}C$ and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity ($1.53^{\circ}C$) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs.

저온 유도 시스템을 가진 재조합 대장균을 이용한 남극 세균 Flavobacterium frigoris PS1 유래 결빙방지단백질의 Pilot-scale 생산