• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2019년도 하계 학술발표초록집
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• pp.247-248
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• 2019

• 미생물학회지
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• 제46권1호
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• pp.73-79
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• 2010

극지연구소(KOPRI)는 국내외적으로 유일하게 남북극 지역에서 분리한 저온적응성 박테리아 균주를 대상으로 culture collection(약 6,300균주)을 구축하여 운영하고 있다. 보유 중인 프로테아제(protease) 생산 균주들(총 874균주) 중에서 활성이 높은 프로테아제를 생산하는 78개의 균주들을 1차 선발한 후, 1% skim milk가 포함된 0.1${\times}$ ZoBell 고체배지에 접종하고 다양한 온도($5-30^{\circ}C$)에서 배양하면서 세포외분비성 프로테아제의 활성을 비교하였다. 위의 신속하고 직접적인 균주 스크리닝 방법을 통해서, 최종적으로 저온활성 프로테아제를 생산하는 15개의 저온적응성 균주들을 선발하였다. 최종 선발된 균주들은 16S rRNA 유전자의 분석결과 Pseudoalteromonas (13균주)와 Flavobacterium (2균주) 속(genus)으로 분류되었고, $5-15^{\circ}C$ 저온에서도 활성을 나타내는 저온성 프로테아제를 생산하였다. 15개 균주들이 생산하는 각각의 프로테아제는 특이적 화합물에 의한 효소활성 억제 정도에 따라 5개의 그룹(serine protease, aspartic protease, cysteine protease, metalloprotease, 그리고 미분류 프로테아제)으로 분류되었다. 본 실험을 통해서 선발한 남북극 유래 박테리아 균주들은 새로운 저온활성 프로테아제를 발굴하기 위한 유용한 생물자원으로서의 가치를 가지고 있다.

• ALGAE
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• 제34권4호
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• pp.267-275
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• 2019

Three new species of the genus Micractinium were collected from five localities on the South Shetland Islands in maritime Antarctica, and their morphological and molecular characteristics were investigated. The vegetative cells are spherical to ellipsoidal and a single chloroplast is parietal with a pyrenoid. Because of their simple morphology, no conspicuous morphological characters of new species were recognized under light microscopy. However, molecular phylogenetic relationships were inferred from the concatenated small subunit rDNA, and internal transcribed spacer (ITS) sequence data indicated that the Antarctic microalgal strains are strongly allied to the well-supported genus Micractinium, including M. pusillum, the type species of the genus, and three other species in the genus. The secondary structure of ITS2 and compensatory base changes were used to identify and describe six Antarctic Micractinium strains. Based on their morphological and molecular characteristics, we characterized three new species of Micractinium: M. simplicissimum sp. nov., M. singularis sp. nov., and M. variabile sp. nov.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2008년도 한국우주과학회보 제17권2호
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• pp.25.2-25.2
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• 2008

Polar rain is a spatially uniform precipitation of electrons with energies around 100eV that penetrate into the polar cap region where geomagnetic field lines are connected to the Interplanetary Magnetic Fields (IMF). Since their occurrences depend on the IMF sector polarity, they are believed to originate from the field aligned component of the solar wind. However, statistically direct correlation between polar rain and solar wind has not been shown. In this presentation, we examined specifically the IMF strength influence on the polar rain flux variation by classifying of IMF sector polarities. For this study, we employed the polar rain flux data measured by STSAT-1 and compared them with the solar wind parameters obtained from the WIND and ACE satellites. We found the direct mutuality between polar rain flux and IMF strength with correlation coefficient above 0.5. This proportional tendency appears stronger when the northern hemisphere is in the away sector of the IMF, which could be associated with a favorable geometry for magnetic reconnection. Simple particle trajectory simulation clearly shows why polar rain intensity depends on the IMF sector polarity. These results are consistent with the direct entry model of Fairfield et al.(1985), while low correlation coefficient with solar wind density, the similarity between slops of both energy spectra shows that transport process occur without acceleration.

• KSBB Journal
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• 제27권2호
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• pp.109-113
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• 2012

This research was to examine the effects of various cyclodextrins on the solubility and stability of prodigiosin in seawater. Among them, ${\beta}$-cyclodextrin was found to have the best efficiency and formation of the inclusion complex was saturated when prodigiosin and ${\beta}$-cyclodextrin were mixed in a ratio of 1:8 and shaken at $25^{\circ}C$ and pH 8.0 for 6 h. The maximum algicidal activity against Chattonella antiqua using the inclusion complex stored at $4^{\circ}C$ for 5 weeks of culture was obtained, $52.28{\pm}3.41%$, which was about 5.0 fold higher than that of control. Our results suggest that inclusion complexes of prodigiosin and ${\beta}$-cyclodextrin could serve as effective algicidal agents.

• ALGAE
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• 제21권2호
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• pp.169-174
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• 2006

We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.

• Bulletin of the Korean Chemical Society
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• 제27권5호
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• pp.718-724
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• 2006

The heterogeneous association behaviour of various concentration binary mixtures of mono alkyl ethers of ethylene glycol with ethyl alcohol were investigated by dielectric measurement in benzene solutions over the entire concentration range at 25 ${^{\circ}C}$. The values of static dielectric constant $\epsilon_0$ of the mixtures were measured at 1 MHz using a four terminal dielectric liquid test fixture and precision LCR meter. The high frequency limiting dielectric constant $\epsilon_\infty$ values were determined by measurement of refractive index $n_D$ ($\epsilon_\infty\;=\;n_D\;^2$). The measured values of $\epsilon_0$ and $\epsilon_\infty$ were used to evaluate the values of excess dielectric constant $\epsilon^E$, effective Kirkwood correlation factor $g^{eff}$ and corrective correlation factor $g_f$ of the binary polar mixtures to obtain qualitative and quantitative information about the H-bond complex formation. The non-linear behaviour of the observed $\epsilon_0$ values of the polar molecules and their mixtures in benzene solvent confirms the variation in the associated structures with change in polar mixture constituents concentration and also by dilution in non-polar solvents. Appearance of the maximum in $\epsilon^E$ values at different concentration of the polar mixtures suggest the formation of stable adduct complex, which depends on the molecular size of the mono alkyl ethers of ethylene glycol. Further, the observed $\epsilon^E$ < 0 also confirms the heterogeneous H-bond complex formation reduces the effective number of dipoles in these polar binary mixtures. In benzene solutions these polar molecules shows the maximum reduce in effective number of dipoles at 50 percent dilutions. But ethyl alcohol rich binary polar mixtures in benzene solvent show the maximum reduce in effective number of dipoles in benzene rich solutions.

• Reproductive and Developmental Biology
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• 제33권1호
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• pp.29-33
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• 2009

The brilliant cresyl blue (BCB) has been used to select the developmental competent oocytes in pigs, goats and cows. Growing oocytes have a higher level of active glucose-6-phosphate dehydrogenase(G6PDH) compare to mature oocytes and are rarely stained compared to mature oocytes, because G6PDH converts BCB to colorless. First polar body extrusion regard as a guideline of meoisis completion. Selection of polar body extrude oocyte is more developmental competent to blastocyst than unselected. This study was conducted to compare the BCB test to the polar body extrusion on selection of developmental competent porcine oocytes for the production of blastocyst. Cumulus-Oocytes complex were exposed to 26uM BCB stain diluted in NCSU-23 for 90 min. There was no significant difference embryo development to blastocysts between BCB treated and not treated($19.58{\pm}1.99$ vs $18.75{\pm}2.27%$), which means there was no detrimental effect of BCB exposure to oocytes. Normal fertilization is not differed among treatment groups from 70.0 to 78.4% development to blastocyst, beside polyspermy did not. To compare two different selection methods, BCB test and polar body extrusion, evaluate the developmental competent of IVP embryos. BCB+PB+(blue stained and polar body extruded, $20.71{\pm}0.45%$) and BCB-PB+(colorless and polar body extruded, $20.04{\pm}l.29%$) groups are significantly (p<0.05) higher developed than those of BCB+PB-(blue stained and no polar body, $13.24{\pm}0.73%$) and BCB-PB-(colorless and no poladbody, $7.25{\pm}0.77%$). These results showed that selection of polar body extruded oocytes method is more efficient than that of BCB test.

• Journal of Astronomy and Space Sciences
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• 제35권3호
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• pp.185-193
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• 2018

Jang Bogo Station (JBS), the second Korean Antarctic research station, was established in Terra Nova Bay, Antarctica ($74.62^{\circ}S$ $164.22^{\circ}E$) in February 2014 in order to expand the Korea Polar Research Institute (KOPRI) research capabilities. One of the main research areas at JBS is space environmental research. The goal of the research is to better understand the general characteristics of the polar region ionosphere and thermosphere and their responses to solar wind and the magnetosphere. Ground-based observations at JBS for upper atmospheric wind and temperature measurements using the Fabry-Perot Interferometer (FPI) began in March 2014. Ionospheric radar (VIPIR) measurements have been collected since 2015 to monitor the state of the polar ionosphere for electron density height profiles, horizontal density gradients, and ion drifts. To investigate the magnetosphere and geomagnetic field variations, a search-coil magnetometer and vector magnetometer were installed in 2017 and 2018, respectively. Since JBS is positioned in an ideal location for auroral observations, we installed an auroral all-sky imager with a color sensor in January 2018 to study substorms as well as auroras. In addition to these observations, we are also operating a proton auroral imager, airglow imager, global positioning system total electron content (GPS TEC)/scintillation monitor, and neutron monitor in collaboration with other institutes. In this article, we briefly introduce the observational activities performed at JBS and the preliminary results of these observations.

• 한국통신학회논문지
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• 제38C권1호
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• pp.109-117
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• 2013

극 부호(polar codes)는 광범위한 이진 입력 이산 무기억 채널(binary input discrete memoryless channel: BI-DMC)에서 채널 용량에 달성하는 것이 이론적으로 증명된 최초의 채널부호이다. 하지만 유한한 길이를 갖는 극 부호는 연속 제거 리스트(successive-cancellation list: SCL) 복호기에서 오류마루(error floor)가 발생하는 문제점이 있다. 선행 연구에 따르면 이 오류마루 현상은 극 부호에 오류 검출 코드(error detection codes) 중 하나인 CRC(Cyclic Redundancy Check) 부호를 연접했을 때 효과적으로 낮출 수 있는 것으로 알려져 있다. 본 논문에서는 외부 부호(outer codes)를 사용하지 않고 극 부호와 RM(Reed-Muller) 부호의 생성 행렬 연관성을 이용하여 기존 극 부호보다 확장된 최소거리를 갖는 극 부호를 제안한다. 그리고 제안된 극 부호와 CRC 부호를 연접한 극 부호의 성능을 비교한다.