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Postoperative Echocardiographic Hemodynamic Comparison between Recently Available Bileaflet Mechanical Valves (수종의 기계판막치환후 초음파심음향도를 이용한 판막간의 혈류역학적 비교)

  • Kang Joon Kyu;Hong Joon Hwa;Kim Hyung Tai;Park In Duk;Lee Cheol Joo
    • Journal of Chest Surgery
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    • v.38 no.7 s.252
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    • pp.496-500
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    • 2005
  • There was no difference between the bileaflet mechanical valves on the midterm and longterm clinical outcome. We reviewed the hemodynamic comparison between recently available mechanical valves by Doppler Echocardiography. Material and Method: We retrospectively reviewed 396 postoperative hemodynamic datas (EOA, MDPG, and MSPG) by doppler echocardiography in 345 patients. Mechanical valves from 5 venders (Sorin Bicarbon, SJM, ATS, On-X, and Edward MIRA) were compared. There were 232 valves in mitral position, 162 in aortic, and 2 in tricuspid. Result: There were 178 men (mean age; $50.6\pm13.9$ years old) and 167 women $(52.6\pm,4.6)$. MDPG/EOA of 27 mm in mitral position was Sorin; $4.2\pm1.5 mmHg/3.0\pm0.9cm^2,\;SJM;\;2.3\pm1.2/3.5\pm0.6$. In 29mm, Sorin, SJM, ATS, On-X, MIRA revealed $3.4\pm1.2/3.1\pm0.6,\;3.3\pm1.1/2.7\pm0.4,\;3.8\pm0.8/3.2\pm0.6,\;4.0\pm3.0/3.1\pm0.9,\;2.9\pm0.9/3.0\pm0.8$ In 31mm, Sorin, SJM, ATS, MIRA revealed $3.9\pm1.9/2.9\pm0.6,\;3.5\pm1.2/3.0\pm0.6,\;3.4\pm0.8/2.8\pm0.2,\;3.7\pm1.5/2.7\pm0.7$. In 33mm, Sorin, SJM, MIRA revealed $4.4\pm0.9/2.5\pm0.4,\;3.4\pm1.5/3.3\pm0.5,\;4.7\pm2.4\3.0\pm0.3$. MSPG/EOA of 19mm aortic position was Sorin, SJM, ATS, On-X, MIRA $18.0 mmHg/1.2cm^2,\;25.6\pm8.7/1.1\pm0.3,\;25.9\pm12.6/1.2\pm0.3,\;23.0/1.3,\;27.9\pm7.1/1.2\pm0.1$ in that order. In 21mm, SJM, ATS, On-X, MIRA revealed $18.3\pm6.7/1.5\pm0.5,\;13.7\pm2.1/1.7\pm0.3,\;17.0/1.4,\;17.1\pm5.5/1.8\pm0.5$. In 23mm Sorin, SJM, ATS, On-X, MIRA revealed $14.0\pm4.6/1.7\pm0.6,\;12.8\pm3.2/2.0\pm0.2,\;16.8\pm12.2/2.1\pm0.9,\;14.0/1.5,\;15.0\pm5.5/1,8\pm0.5$. In 25mm, SJM and MIRA revealed $14.0\pm5.1/1.8\pm1.0,\;11.0/2.3$. There was no statistically significant difference in these values between the venders given the same position and size. 2 redo valve replacements were performed, 1 due to severe hemolysis in ATS and 1 due to leaflet immobilization in SJM. Conclusion: Postoperative hemodynamic comparison by doppler echocardiography shows no statistically significant difference between recently available mechanical valves in this country.

Clinical Review of Prosthetic Heart Valve Replacement (인공심장판막치환술의 임상적 고찰)

  • 장기경;윤후식
    • Journal of Chest Surgery
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    • v.29 no.9
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    • pp.977-982
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    • 1996
  • Cardiac valve implantation was performed in 107 patients from September, 1988 to May, 1995. There were 3) men and 74 women, whose ages ranged from 19 to 75 years(mean 42.6$\pm$11.7). Mitral valve was implanted in 61 patients, double(mitral & aortic) valve were Implanted in 28 patients and aortic valve was implanted in 18 patients. Follow up was 100% complete, with 345.6 patient-years and a mean fo low up of 41 months(from 1.5 to 84 months). The total mortality was 14.9%(16 patients). The early mortality was 5.6%(6 patients) and the late mortality was 9.3%(10 patients). The overall actuarial survival was 92.6 $\pm$ 2 6% at 2 years, 88.6$\pm$3.8% at 6 years. The probability of freedom from valve failure, thromboembolism and bacterial endocarditis were 388.6 $\pm$ 3.8, 88.3 $\pm$3.9, 89.5 $\pm$3.7 at 6 years, respectively.

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Studies on Electrocardiogram of the Normal Korean Native Goat II. Waveforms and Amplitudes of the Unipolar Precordial Chest Leads (정상적인 한국 흑염소의 심전도에 관한 연구 II. 담부단극유도의 파형과 전위)

  • 최인혁;김기주;윤여백;서석열;김남수
    • Journal of Veterinary Clinics
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    • v.14 no.2
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    • pp.338-348
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    • 1997
  • The electrocardiographic (ECG) parameters on unipolar precordial chest leads in the normal Korean native goat of 343 heads as to CV$_{6}$LU, CV$_{6}$LL CV$_{6}$RU, CV$_{6}$RL and V10 have been measured with a 3-channel Elertrocardiograph and computed, analysed. All wave types as positive, negatives biphasic and flatting in the P and T waves appeared in all leads but any special wave type was not shown more than 60%. Average amplitudes with the highest frequent rate in P wave were 81.4$\pm$32.0 $\mu $V (52.3%), 59.6$\pm $ 27.5 $\mu $V (50.5 %) of Positive type in leads CV$_{6}$LU and CV$_{6}$LL, and -5fl.5$\pm $22.6 $\mu $V (44.0%) of negative type in leads VIO, and 51.3% and 44% of flatting type in leads CV$_{6}$RU and CV$_{6}$RL, but flatting type of clinic form appeared frequent rate between 53% and 77% in all leads. In T wave, average amplitudes with the highest frequent rate were 265.0$\pm $97.1 $\mu $V (54.0%) and 212.2$\pm $90.7 $\mu $V (57.0%) of positive type in leads CV$_{6}$LL and CV$_{6}$RL, and -252.8$\pm $90.7 $\mu $V (56.6%) of negative in lead V10, but amplitudes of T waves that positive and negative type appeared similarity frequent ra蛇 ware 208.7$\pm $99.7 $\mu $V (42.1%), -159.0$\pm $81.6 (43.8%) in lead CV$_{6}$LU and 153.2$\pm $139.0 $\mu $V (47.3%), -130.0$\pm $81.4 (43.2%) in lead CV$_{6}$RU, Amplitudes of QRS complex wave forms showed the highest frequent rate were 218.2$\pm $96.4 $\mu $V (47.8%), 308.3$\pm $135.2 $\mu $V (46.8 %), 232.8$\pm $126.5 $\mu $V (58.5%) and 225.3$\pm $89.6 $\mu $V (54.9%) of R type in the leeds CV$_{6}$LU, CV$_{6}$LL, CV$_{6}$RU and CV$_{6}$RL, and were -92.5$\pm $79.1 $\mu $V,479.0 $\pm $116.6 $\mu $V (33.2%) and 212.1 $\pm $86.7 $\mu $V (32.8%) of QR and 05 type in the lead V10. These results in the V10, CV$_{6}$RU, CV$_{6}$RL, CV$_{6}$LU, CV$_{6}$LL. may be not starve to purpose of unipolar precordial chest leads.

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Evaluation of the Neural Fiber Tractography Associated with Aging in the Normal Corpus Callosum Using the Diffusion Tensor Imaging (DTI) (확산텐서영상(Diffusion Tensor Imaging)을 이용한 정상 뇌량에서의 연령대별 신경섬유로의 변화)

  • Im, In-Chul;Goo, Eun-Hoe;Lee, Jae-Seung
    • Journal of the Korean Society of Radiology
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    • v.5 no.4
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    • pp.189-194
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    • 2011
  • This study used magnetic resonance diffusion tensor imaging (DTI) to quantitatively analyze the neural fiber tractography according to the age of normal corpus callosum and to evaluate of usefulness. The research was intended for the applicants of 60 persons that was in a good state of health with not brain or other disease. The test parameters were TR: 6650 ms, TE: 66 ms, FA: $90^{\circ}$, NEX: 2, thickness: 2 mm, no gap, FOV: 220 mm, b-value: $800s/mm^2$, sense factor: 2, acquisition matrix size: $2{\times}2{\times}2mm^3$, and the test time was 3 minutes 46 seconds. The evaluation method was constructed the color-cored FA map include to the skull vertex from the skull base in scan range. We set up the five ROI of corpus callosum of genu, anterior-mid body, posterior-mid body, isthmus, and splenium, tracking, respectively, and to quantitatively measured the length of neural fiber. As a result, the length of neural fiber, for the corpus callosum of genu was 20's: $61.8{\pm}6.8$, 30's: $63.9{\pm}3.8$, 40's: $65.5{\pm}6.4$, 50's: $57.8{\pm}6.0$, 60's: $58.9{\pm}4.5$, more than 70's: $54.1{\pm}8.1mm$, for the anterior-mid body was 20's: $54.8{\pm}8.8$, 30's: $58.5{\pm}7.9$, 40's: $54.8{\pm}7.8$, 50's: $56.1{\pm}10.2$, 60's: $48.5{\pm}6.2$, more than 70's: $48.6{\pm}8.3mm$, for the posterior-mid body was 20's: $72.7{\pm}9.1$, 30's: $61.6{\pm}9.1$, 40's: $60.9{\pm}10.5$, 50's: $61.4{\pm}11.7$, 60's: $54.9{\pm}10.0$, more than 70's: $53.1{\pm}10.5mm$, for the isthmus was 20's: $71.5{\pm}17.4$, 30's: $74.1{\pm}14.9$, 40's: $73.6{\pm}14.2$, 50's: $66.3{\pm}12.9$, 60's: $56.5{\pm}11.2$, more than 70's: $56.8{\pm}11.3mm$, and for the splenium was 20's: $82.6{\pm}6.8$, 30's: $86.9{\pm}6.4$, 40's: $83.1{\pm}7.1$, 50's: $81.5{\pm}7.4$, 60's: $78.6{\pm}6.0$, more than 70's: $80.55{\pm}8.6mm$. The length of neural fiber for normal corpus callosum were statistically significant in the genu(P=0.001), posterior-mid body(P=0.009), and istumus(P=0.012) of corpus callosum. In order of age, the length of neural fiber increased from 30s to 40s, as one grows older tended to decrease. For this reason, the nerve cells of brain could be confirmed through the neural fiber tractography to progress actively in middle age.

Distribution of T and B lymphocytes in peripheral blood and lymphoid tissues of bovine (소의 순환혈액 및 림프조직내 T 및 B 림프구 분포)

  • Yoon, Chang-yong;Kim, Tae-Joong;Chai, Hyo-seok;Kim, Jong-Myeog;Song, Hee-jong
    • Korean Journal of Veterinary Research
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    • v.31 no.1
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    • pp.71-75
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    • 1991
  • This study was undertaken to identify the distribution of T and B lymphocytes in bovine peripheral blood and various lymphoid tissues by the method of ABPC using RABTS, $BLT_1$ and $_6E_{12}$ as primary antibodies. RABTS, $BLT_1$ and $_6E_{12}$ positive cells in PB-MNCs were 70.9${\pm}5.5%$, $59.0{\pm}8.7%$ and $23.0{\pm}8.7%$, respectively. $BLT_1$ and $_6E_{12}$ positive cells in nylon wool nonadherent cells of PB-MNCs were $91.6{\pm}1.0%$ and $9.6{\pm}0.8%$, respectively. In the lymphoid tissues such as inguinal lymph node, mesenteric lymph node, spleen and thymus, positive cells of RABTS were $76.3{\pm}3.4%$, $74.2{\pm}8.2%$, $73.6{\pm}5.5%$ and $95.6{\pm}2.8%$, those of $BLT_1$ were $56.4{\pm}6.2%$, $55.6{\pm}7.7%$, $48.6{\pm}5.1%$ and $23.0{\pm}4.8%$ and those of $_6E_{12}$ were $45.3{\pm}7.4%$, $42.3{\pm}5.8%$, $48.5{\pm}6.2%$ and $5.6{\pm}2.1%$, in order. These results are indicating that nylon wool column method is effective for separation of bovine ocytes.

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Effect of Exercise and Diet Control Program on Nitrogen Balance in Obese Children (단기간의 운동 및 식이 조절이 비만아동의 질소 평형에 미치는 영향)

  • 박소앙;이성숙;최인선;오승호
    • Journal of Nutrition and Health
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    • v.34 no.6
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    • pp.637-644
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    • 2001
  • The purpose of this study was to examine the effect of exercise and diet control program on nitrogen balance in obese children. The subjects of this study were five obese boys aged from 8 to 12 participating in the control period(C) for 3 days, exercise without energy deficit period(EEN) for one week, and exercise with energy deficit period(EED) far another one week, consecutively. The intensity of the exercise was 60-70% of HRmax and the energy was restricted at 493 kcal/day. Daily mean total energy intake was 2,152 $\pm$ 138kca1, 1,861 $\pm$ 138kca1, and 1,368 $\pm$ 87kca1 for the period C, EEN and EED, respectively. The body weight for EEN and EED decreased compared with the period C by average 1.2 $\pm$ 0.2kg and 1.8 $\pm$ 0.3kg, respectively. Of the body weight loss, the fat mass decreased bur 1.0 $\pm$ 0.3kg and 1.5 $\pm$ 0.5kg and the lean body mass by 0.2 $\pm$ 0.4kg and 0.3 $\pm$ 0.4kg, respectively. Of the daily mean nitrogen consumption was 9.1 $\pm$ 0.7g, 9.1 $\pm$ 0.8g and 7.1 $\pm$ 0.5g, the daily mean nitrogen excretion from feces was 1.3 $\pm$ 0.2g, 0.9 $\pm$ 0.1g and 0.7 $\pm$ 0.1g, and from urine was 6.7 $\pm$ 0.6g, 6.6 $\pm$ 0.5g and 6.7 $\pm$ 0.6g, for the period C, EEN and EED, respectively. Daily mean nitrogen balance excluding nitrogen excretion firm feces and urine indicated + 1.1 $\pm$ 1.0, +1.6 $\pm$ 0.6 and - 0.3 $\pm$ 0.9 for the period C, EEN and EED, respectively. Despite the negative indication during EED, there was no statistical signifiranre. The daily mean creatinine excretion was 0.5 $\pm$ 0.0g, 0.7 $\pm$ 0.1g and 0.6 $\pm$ 0.0g and the creatinine-height index(CHl) was 85.1 $\pm$ 6.7%, 83.8 $\pm$ 6.6% and 81.0 $\pm$ 6.5%, for the period C, EEN and EED, respectively, indicating little significant difference among the periods. Taking above results into consideration, it seems the exercise program conducted for this study did not substantially affect the nitrogen balance of the obese children, and may be used far the body weight control. Howeever, the adequacy of the energy restriction and amount of exercise applied to this study need to be further investigated as the nitrogen balance indicated negative and increasing tendency of the creatinine excretion showed during the EED compared with period C, despite the statistical insignificance.

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Studies on Taste Components of Traditional Kochujang (전통 고추장의 맛성분)

  • Shin, Dong-Hwa;Kim, Dong-Han;Choi, Ung;Lim, Dae-Kwan;Lim, Mi-Sun
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.152-156
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    • 1996
  • Taste components of traditional kochujang from 55 households were investigated. The major free sugars in traditional kochujang (fermented hot pepper-soybean paste) were glucose $(8.21{\pm}5.62%)$ and maltose $(6.95{\pm}7.27%)$ and the minors were fructose $(1.88{\pm}1.27%)$ and sucrose $(1.05{\pm}1.21%)$. Succinic $(901.83{\pm}826.23\;mg%)$, citric $(484.16{\pm}242.89\;mg%)$ and lactic $(381.63{\pm}367.88\;mg%)$ acids in traditional kochujang were found in large amounts and acetic, oxalic, and formic acids in smaller amounts. The traditional kochujang contained large amounts of proline $(10.66{\pm}6.27\;mg%)$, glutamic acid $(9.27{\pm}10.97\;mg%)$, aspartic acid $(9.14{\pm}5.84\;mg%)$, lysine $(6.19{\pm}6.66\;mg%)$, and serine $(5.72{\pm}3.79\;mg%)$, and the total free amino acid content was 64.35mg%. Among the nucleotides and their related compounds in traditional kochujang, CMP $(42.90{\pm}28.16\;mg%)$ were the most abundant major compounds and hypoxanthine $(6.86{\pm}3.45\;mg%)$, IMP $(5.59{\pm}5.84\;mg%)$, inosine $(4.58{\pm}6.91\;mg%)$ and GMP$(3.36{\pm}3.93\;mg%)$ were found in smaller amounts, and AMP and UMP were also found in minor.

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A Study of the Insulin and the C-Peptide Responses to Oral Glucose Load in Nondiabetic and Diabetic Subjects (정상인(正常人) 및 당뇨병환자(糖尿病患者)에서의 경구당부하시(經口糖負荷時) 혈중(血中) Insulin과 C-Peptide의 변동(變動))

  • Lee, Myung-Chul;Choi, Sung-Jae;Kim, Eung-Jin;Min, Hun-Ki;Koh, Chang-Soon
    • The Korean Journal of Nuclear Medicine
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    • v.11 no.1
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    • pp.17-32
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    • 1977
  • The present study was undertaken to evaluate the significance of the insulin and the C-peptide rseponse to oral glucose loads in normal and diabetic subjects and to establish the effects of the obesity. In this study, the authors have measured plasma insulin and C-peptide by means of radioimmunoassay in 10 nonobese normal, 5 obese normal, 13 nonobese moderate diabetic patients, 9 obese moderate diabetic patients and 9 severe diabetic patients. The results obtained were as follows; 1. In 10 nonobese normal subjects, the plasma insulin level at fasting state and at 30, 60, 90, and 120 min after oral glucose loads were $15.7{\pm}3.4,\;48.3{\pm}9.8,\;40.4{\pm}6.7,\;37.4{\pm}6.5\;and\;26.0{\pm}4.2uU/ml(Mean{\pm}S.E.)$ and C-peptide were $1.9{\pm}0.3,\;3.9{\pm}0.6,\;6.3{\pm}0.6,\;5.7{\pm}0.5\;and\;4.0{\pm}0.5ng/ml$. The change of C-peptide was found to go almost parallel with that of insulin and the insulin value reaches to the highest level at 30 min whereas C-peptide reaches to its peak at 60min. 2. The plasma insulin level in 5 obese normal subjects were $38.9{\pm}12.3,\;59.5{\pm}12.3,\;59.2{\pm}17.1,\;56.1{\pm}20.0\;and\;48.4{\pm}17.2uU/ml$ and the C-peptide were $5.5{\pm}0.4,\;6.8{\pm}0.5,\;7.9{\pm}0.8,\;7.9{\pm}0.8\;and\;7.8{\pm}2.0ng/ml$. The insulin response appeared to be greater than nonobese normal subjects. 3. In 13 nonobese moderate diabetic patients, the plasma insulin levels were $27.1{\pm}4.9,\;44.1{\pm}6.0,\;37.3{\pm}6.6,\;35.5{\pm}8.1\;and\;34.7{\pm}10.7uU/ml$ and the C-peptide levels were $2.7{\pm}0.4,\;4.9{\pm}0.7,\;6.5{\pm}0.5,\;7.0{\pm}0.3\;and\;6.7{\pm}1.0ng/ml$. There was little significance compared to nonobese normal groups but delayed pattern is noted. 4. In 9 obese moderated diabetic patients, the plasma insulin levels were $22.1{\pm}7.9,\;80.0{\pm}19.3,\;108.0{\pm}27.0,\;62.0{\pm}17.6\;and\;55.5{\pm}10.1uU/ml$ and the C-peptide levels were $5.2{\pm}0.4,\;8.0{\pm}1.0,\;10.4{\pm}1.6,\;10.4{\pm}1.7\;and\;10.1{\pm}1.0ng/ml$ and its response was also greater than that of nonobese moderate diabetic patients. 5. The plasma insulin concentrations in 9 severe diabetic subjects were $8.0{\pm}3.8,\;12.1{\pm}3.5,\;16.8{\pm}4.6,\;19.6{\pm}5.2\;and\;15.0{\pm}5.0uU/ml$ and the C-peptide levels were $1.6{\pm}0.3,\;2.4{\pm}0.4,\;4.1{\pm}0.6,\;4.0{\pm}0.8\;and\;4.5{\pm}0.7ng/ml$ and the insulin and C-peptide responses were markedly reduced in severe diabetic groups. 6. There were-significant differences between each groups of patients on the magnitude of total insulin or C-peptide areas, the insulinogenic index and the C-peptide index.

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A Survey on Total Sugar, Sodium and Artificial Sweetener Contents of Light Meals from the School Zone in Gwangju (광주지역 학교주변 길거리 음식 중 당, 나트륨, 인공감미료 함량 실태조사)

  • Yang, Yong-Shik;Kim, Jong-Pil;Gang, Gyung-Lee;Seo, Kye-Won;Cho, Bae-Sick;Hong, Sam-Jae;Choi, Gye-Sun;Kim, Eun-Sun;Park, Jong-Tae
    • Journal of Food Hygiene and Safety
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    • v.24 no.4
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    • pp.385-390
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    • 2009
  • This survey was conducted to monitor the total sugar, sodium, and artificial sweetener contents of light meals from the school zone in Gwangju, from November, 2008 to April, 2009. A total of 100 samples were tested. HPLC/ELSD was used for the determination of total sugar contents and AAS for sodium contents. Total sugar amounts were the sum of both mono-and disaccharide according to nutritional information standard defined by the Korea Food and Drug Administration. The results were as follows by the form of total sugar content(sodium content): $5.7\;{\pm}\;1.9%$($4.6\;{\pm}\;1.6\;mg/g$) for Tteokbokki, $7.6\;{\pm}\;5.1%$($5.4\;{\pm}\;2.7\;mg/g$) for Chicken(skewed), $15.4\;{\pm}\;3.2%$($3.0\;{\pm}\;0.9\;mg/g$) for Bun(fish-shaped), $0.6\;{\pm}\;0.3%$($3.7\;{\pm}\;1.0\;mg/g$) for Sundae, $0.9\;{\pm}\;0.3%$($7.4\;{\pm}\;1.4\;mg/g$) for Oden(with broth), $20.5\;{\pm}\;6.2%$($2.9\;{\pm}\;0.6\;mg/g$) for Waffle, $6.8\;{\pm}\;2.2%$($4.7\;{\pm}\;1.0\;mg/g$) for Hotdog, $14.2\;{\pm}\;2.8%$($3.1\;{\pm}\;1.6\;mg/g$) for Hotteok, $6.6\;{\pm}\;2.1%$($3.9\;{\pm}\;0.6\;mg/g$) for Toast, $10.1\;{\pm}\;3.7%$($2.3\;{\pm}\;0.7\;mg/g$) for Fry(sweet potato), $1.6\;{\pm}\;0.7%$($4.0\;{\pm}\;0.7\;mg/g$) for Fry(etc), and $9.3\;{\pm}\;2.4%$($4.0\;{\pm}\;0.6\;mg/g$) for Doughnut. In addition, sodium saccharin, one of the artificial sweeteners, was tested. Among 60 samples from February to April, 2009, 11 samples had sodium saccharin(6.7~101.0 mg/kg).

Selection of superior breeding colonies of Apis mellifera based on performance-testing (능력검정에 의한 우량 종봉 선발에 관한 연구)

  • Son, Young-Ik;Cha, Yong-Ho;Choi, Kwang-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.19
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    • pp.17-22
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    • 2001
  • The study was conducted to establish the method of performance test of honeybee queens under Korean environment. Colony performances of Apis mellifera were tested based on wintering ability, colony population and honey yield in Sangju, Korea from November, 1999 to October, 2001. The results of performance-testing on the honeybee colonies are summarized as follows: The colony weights measured before and after wintering in 1999~2000 season were averaged $22.6{\pm}2.6kg$ and $20.1{\pm}2.6kg$, respectively, showing the decrease of $2.5{\pm}0.7kg$. During 2000~2001 winter season, the colony weight before wintering was $22.6{\pm}2.3kg$ and was decreased by $2.3{\pm}0.6kg$. The number of combs well covered with bees was $13.1{\pm}4.6$ as measured on the 22nd of May. 2000 and $16.0{\pm}3.8$ on the 21st of May. 2001, respectively. The rate of supering colonies were 50% and 98.5% in 2000 and 2001, respectively. The amount of honey production was $12.7{\pm}8.6kg$ per colony in 2000, and $14.2{\pm}7.6kg$ per colony with unselected queens and $23.5{\pm}4.9kg$ per colony with selected-tested queens in 2001, which were harvested two times during main nectar flow season of black locust, Robinia peseudoacasia.

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