• Journal of Embryo Transfer
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• v.10 no.3
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• pp.203-208
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• 1995

This study was carried out to efficiently use the ultrarapid freezing method in the cryopreservation of mouse ova. For this, the effects of dehydration method, oval vigour and $0^{\circ}C$ controlling method on post-thawing viability were investigated. Fresh mouse ova were dehydrated in mPBS with 3.5M DMSO and /or 0.25M sucrose, and directly immersed in L$N_2$ for ultrarapidly freezing. The frozen ova were thawed at 37$^{\circ}C$, rehydrated in mPBS with 0.25M sucrose, and then repeatedly washed in HAM's Fl0 before evaluating the morphological normality of frozen-thawed ova. The results obtained showed that there was difference between treatments in a experiment. 1) The post-thawing viability of ova dehydrated in multi-step (48.4$\pm$13.8%) was higher than that of ova in two-step (40.9$\pm$14.0%). 2) The post-thawing viability of fertilized ova (87$\pm$14.0%) was significantly(p<0.0l) higher than that of unfertilized ova (5.4$\pm$5.4%). 3) The post-thawing viability of ova dehydrated and rehydrated using a cooling machine (95.8$\pm$4.2%) was significantly(p<0.05) higher than that on ice(84.1$\pm$9.9). In conclusion, in order to efficiently cryopreserve ova in vitro with ultrarapidly freezing method, highly viable embryos should be selected, heavy osmotic shock to the dehydrating ova should be avoided, and embryos in high osmotic condition were dehydrated and rehydrated in a constantly low temperature.

• Journal of Korean Society for Atmospheric Environment
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• v.33 no.4
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• pp.342-350
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• 2017

We report mass concentration and size fraction of TPM, $PM_{10}$ and $PM_{2.5}$ according to Korea standard test method (ES 01301.1 and ES 01317.1) and ISO 23210 methods. Particulate matters were sampled in large stationary emission sources such as a coal power plant and B-C oil refinery. The Korea standard test method PM mass concentrations showed 3~3.5 times larger than the cascade impactor method. On the other hand, the size fraction results showed less than 5% difference (i.e. $PM_{2.5}/PM_{10}$) between two methods. Moreover, the correlation coefficient ($r^2$) is 0.84 between TPM results of the Korea standard test method and CleanSYS. These results suggested not only improvement of current test criteria in terms of technical and theoretical aspects. Further, additional measurements are required in various large stationary sources to compare current field data.

• Clinical and Experimental Pediatrics
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• v.52 no.3
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• pp.351-355
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• 2009

Purpose : This study compared bone ages measured by the Greulich-Pyle (GP) and Tanner-Whitehouse 3 (TW3) methods and investigated the differences in predicted adult heights measured by Bayley-Pinneau (BP) and TW3 methods. Methods : Bone ages were assessed from left-wrist radiographs by two investigators, one for each GP and TW3 methods in 85 normal children, 30 precocious puberty girls, and 30 constitutional growth delay boys. The differences between the measured predicted adult heights using the BP and TW3 methods were compared in each group. Results : The bone age measured by the TW3 method was less than that by the GP method in normal children. The predicted adult heights measured by the two methods showed no significant difference in normal boys, while the predicted adult height measured by the TW3 method was higher than that by the BP method for normal girls ($156.4{\pm}4.7$ cm vs. $158.9{\pm}3.8$ cm, P<0.01) and for precocious puberty girls ($156.3{\pm}4.0$ cm vs. $159.3{\pm}4.2$ cm, P<0.01). In contrast, the predicted adult height was higher from the BP method than from the TW3 method in constitutional growth delay boys ($173.3{\pm}4.4$ cm vs. $169.7{\pm}3.2$ cm, P<0.01). Conclusion : There were significant differences in predicted adult heights between the BP and TW3 method in normal girls, precocious puberty girls, or constitutional growth delay boys. In precocious puberty and constitutional growth delay, the BP method might be preferred to predict adult height, but further studies on final adult height are needed.

• Korean Journal of Clinical Laboratory Science
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• v.40 no.2
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• pp.113-117
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• 2008

It designed a study to examine the efficiency of DNA and RNA extraction from archival formalin-fixed, paraffin-embedded tissues using an non-heating and heating method. Archival paraffin blocks of liver, kidney, colon were randomly selected. Each paraffin block was prepared in 20 microtubes. For each paraffin blocks were tested non-heating DNA extraction to 10 microtubes and heating protocol under pH 7.0 and $100^{\circ}C$ to 10 microtubes. Evaluation of the results of DNA extraction was carried out by measuring concentration by UV spectrophotometry and then PCR amplification. DNA extraction content that non-heating method was liver $5{\pm}0.7{\mu}g/mL$, kidney $2{\pm}0.3{\mu}g/mL$, colon $6{\pm}0.4{\mu}g/mL$ and heating method was liver $12{\pm}0.6{\mu}g/mL$, kidney $7{\pm}0.5{\mu}g/mL$, colon $10.{\pm}0.3{\mu}g/mL$. Successful RNA extraction was observed, by ${\beta}$-actin amplification, in 46.7% sections for samples treated by the heating method versus 30.0% using non-heating DNA extraction. The extracted nucleic acid showed better values for samples heated at $100^{\circ}C$. Therefore heating extraction of nucleic acid is reliable, quick and efficiency.

• Journal of Pharmaceutical Investigation
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• v.30 no.2
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• pp.99-105
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• 2000

This study compared the permeability of $[^3H]taurine,\;[^3H]phenylalanine,\;and\;[^3H]oxytocin$ through the blood-brain barrier (BBB) in mice and rats with common carotid artery perfusion (CCAP) method that modified internal carotid artery perfusion (ICAP) method. External carotid artery (ECA) was cannulated with coagulating pterygopalatine artery (PPA) in ICAP method, while CCA was cannulated without coagulating PPA in CCAP method. Also, for evaluation of BBB permeability of drugs in mice and rats, we used intravenous injection technique. The results of CCAP method in mice at a perfusion flow-rate of 2 ml/min, the brian volume of distribution $(V_D)$ of $[^{14}C]sucrose,\;[^3H]taurine,\;[^3H]phenylalanine,\;and\;[^3H]oxytocin$ were similar to the result of ICAP method in rats at perfusion flow rate of 4 ml/min. The area under the plasma concentration-time curve and brain uptake of $[^3H]taurine$ by intravenous injection technique, were $65.5{\pm}9.7%ID^*min/ml\;and\;0.515{\pm}0.093%ID/g$, respectively, in mice, and the corresponding values were $8.00{\pm}0.03%ID^*min/ml\;and\;0.052{\pm}0.003%ID/g$ in rats. But the BBB permeability surface-area product of $[^3H]taurine$ was similar between mice and rats. In conclusion, the CCAP method in mice was simple, fast and comparable to ICAP method in rats for drug permeability through the BBB.

• Journal of Korean Public Health Nursing
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• v.16 no.2
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• pp.445-453
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• 2002

Purpose: This study has been designed to evaluate occupational health services on workers with hypertension Method: Study participants were 108 workers having a diagnosed hypertension. The instrument used for this study was the questionnaire developed by Cho(1998). Analysis of the data was done with SPSS Win 10.0 for descriptive statistics, Correlation coefficient. Results: 1. The mean score of process domain was $3.77\pm.43$ The score of process domains were usefulness of health management$(3.98\pm.49)$, education or counselling$(3.87\pm.53)$, follow up in medical examination$(3.79\pm.58)$, usefulness of providing materials$(3.53\pm.62)$, perception of medical examination $(3.37\pm.80)$ 2. The mean score of outcome domain was $3.47 \pm.44$ The score of outcome domains were obstructiveness of social life$(3.90\pm.78)$, habits of living $(3.41\pm.76)$, blood pressure change $(3.25\pm.81)$, perception of seriousness$(3.02\pm.88)$. 3. Blood pressure change was significant correlated with education or counselling(r=.31) and usefulness of providing materials(r=.28).

• Journal of the Korea Convergence Society
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• v.3 no.1
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• pp.27-34
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• 2012

After Fukushima Nuclear Accident on Mar11, 2011, to grasp the thought of university students in Korea on radiation and medical radiation and seek for the right mass communication on the radiation safety of the people and the proper teaching method on radiation, 790 questionnaires from the universities which had a four-year department of radiological science in 5 provinces(Gyeonggi, Gangwon, Chungcheong, Jeolla, and Gyeongsang-do) all over the country were collected and analyzed. The questionnaire was composed of 36 items, and it was analyzed that one of the important causes that made them feel that radiation was dangerous was 'even if they were exposed to the small quantity of radiation, they could have trouble later.' ($3.28{\pm}1.05$). In the item of the control of radiation, there were the respondents who answered that the government should take action rather than an individual($3.87{\pm}0.89$). In the item of 'Fukushima Nuclear Accident made me think that 'We should not keep nuclear power generation', the result was $2.79{\pm}0.95$. In the item of 'My thought on the medical radiation has negatively changed since Fukushima Nuclear Accident', the students who hadn't taken the course related with radiation showed a negative thought on the medical radiation, such as, there was $2.64{\pm}1.02$ as a whole, $2.31{\pm}1.00$ in department of radiological science, $2.94{\pm}0.95$ in department of dental hygiene, and $3.13{\pm}0.82$ in other departments. And also, those who thought that the education of radiation was necessary were 82.28%, and T.V or Internet was thought as the most effective teaching method.

• The Korean Journal of Physiology
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• v.16 no.1
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• pp.69-76
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• 1982

The effect of parathyroid hormone on calcium and phosphate metabolism have been widely investigated, however less attention has been paid to the effect on urinary excretion. This study was performed for the purpose determining urinary excretion of Na, K, Ca, and $Po_4$, of 18 thyroparathyroidectomized (TPTX) rabbits, which were TPTX previously 7 to 10 days compared with the same normal ones. After TPTX 0.2 mg/day of synthyroid was donated to the rabbits. The concentration of electrolytes in the serum and urine was determined by the following method; Na and K were determined by means of flame photometry, Ca was by EDTA titration $method^{19)}$, and $Po_4$ by Fiske and Subba-Raw $method^{20)}$. The results as follows. The concentrations of electrolytes in the serum were 1) In the normal control rabbits (N = 25) (data, $Mean{\pm}S.E.$) $Na\;131.72{\pm}1.33\;mEq/L$, $K\;3.59{\pm}0.28\;mEq/L$, $Ca\;12.58{\pm}0.29\;mg%$, $Po_4\;4.50{\pm}\;0.45mg%$. 2) In the TPTX rabbits(N= 18) $Na\;140.6l{\pm}2.56\;mEq/L$, $K\;3.38{\pm}0.36\;mEq/L$, $Ca\;l2.18{\pm}0.45\;mg%$, $Po_4\;3.92{\pm}\;0.35\;mg%$. There was no significant change between the normal and TPTX rabbits. The concentration of elelctrolytes in the urine were variously changed. 3) In the normal rabbits. $Na\;8.40{\pm}1.09\;mEq/L$, $K\;81.59{\pm}10.19\;mEq/L$, $Ca\;16.02{\pm}3.12\;mg%$, $Po_4\;13.16{\pm}2.89mg%$. 4) In the TPTX rabbits, $Na\;14.57{\pm}3.39\;mEq/L$ slight ncreased, $K\;116.06{\pm}12.77\;mEq/L$ significant increased (P<0.05), $Ca\;18.90{\pm}5.44\;mg%$ no significant increased, $Po_4\;43.38{\pm}8.67\;mg%$ significant increased (p<0.01). The effect of TPTX was assumed that it affected upon increasing tubular secretion of $K^+$ and inhibition of the tubular reabsorption of $Po_4$.

• Particle and aerosol research
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• v.5 no.2
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• pp.53-62
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• 2009

In this study, the continuous monitoring of the concentration of PM-10 atmospheric particulate matter using beta ray attenuation method was compared with gravimetric method from September, 2006 to August, 2007. On the effects of the PM-10 concentration and mass difference by relative humidity and precipitation were considered. The result showed that the measurement error between beta ray method and gravimetric method are within -3~6% in average, which means PM-10 concentration data with beta ray method are relatively comparable. The current study also shows that the high PM-10 concentration events are mainly due to haze, Asian dust, and high relative humidity and the PM-10 mass concentration is closely related with relative humidity and precipitation events. Based on daily mean data, the PM-10 increases as relative humidity increases up to 70~80%, then decreases over 80% due to the precipitation. However, the distinct measurement discrepancy was not shown between beta ray method and gravimetric method based on current results. Consequently, this study shows that the collocated measurement in different instrument is essential in order to quantify the accuracy of PM-10. Furthermore, the more comprehensive and spatially distributed comparison is needed and this is remained for future study.

• Journal of Nutrition and Health
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• v.27 no.8
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• pp.794-804
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• 1994

This study was conducted to investigate the relationship between lactational capacity and intakes of energy and energy-yielding nutrients. Food consumption, intakes of carbohydrate, protein, fat and energy and quantity and proximate composition of milk of 11 lactating Korean mothers were determined at 1, 2 and 3 months postpartum longitudinally. Food consumption was estimated using a 24-hour recall method ; intakes of energy and energy-yielding nutrients were calculated according to the Food Composition Table. Daily milk production was estimated using a 72-hour test-weighing method ; protein, fat, lactose and energy concentrations were analyzed. Average intakes of energy and protein were 1974$\pm$386㎉/day and 67.0$\pm$12.3㎉/day, these were 73% and 74% of the Korean Recommended Allowances, respectively. Average milk yield was 720.1$\pm$123.3ml/day containing energy 59.6$\pm$9.5㎉/dl, protein 1.1$\pm$0.1g/dl, fat 2.7$\pm$0.8g/dl, and lactose 6.3$\pm$0.3g/dl. No relationship existed between the intakes of carbohydrate, protein, fat and energy and the quantity and proximate composition of the milk. However, the intakes of energy, carbohydrate and vegetable protein were inversely related to the concentrations of energy and lactose in the milk. This result indicates that lactational capacity may be affected by the other factors excluding intakes of energy and energy-yielding nutrients and a high intake of energy may not guarantee optimal lactational capacity.