• Korean Journal of Veterinary Service
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• v.25 no.4
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• pp.357-370
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• 2002

This experiment was performed to investigate apoptosis during undergoing patho-genesis of Salmonella gallinarum(SG)-infected chicks. 16 days old, 49 chicks was infected with SG (10$\^$6/-10$\^$8/ CFU/㎖) experimentally, they were autopsied to remove liver, spleen, intestine and lung at 1, 6, 12hr, 1, 2, 4 and 7 day post infection(PI) respectively, for H-E and TUNEL staining. Grossly, white foci in the liver and enlarged spleen were seen on 4 day PI and coppery bronze liver, dark-red discolored intestine, green-yellowish discolored and enlarged spleen was observed on 7 day PI. Histopathologically, multi focal necrosis in the liver, follicle hyperplasia in the spleen and inflammatory cells infiltration in the intestine were shown from 2 day PI and more severely observed on 4 day and 7 day PI. In TUNEL analysis, apoptotic cells reached a maximum at 6hr PI in the liver and intestine and at 12hr PI in the spleen, and then decreased the levels of controls by 7 day PI.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.6
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• pp.1176-1181
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• 2007

In this paper, we consider one of robust control system, fuzzy PI+fuzzy D controller dealing with noise, load, changed parameters of plant. We apply PI+D controller with a design for output of differential function and, we plan fuzzy controller with input for PID parameter of PI+D controller so We design control system meet with the change of environment with robust in relation to change of parameter. Fuzzy control is possessed of easy 4 rules and membership function and We design fuzzy PI+fuzzy D controller. Plant of this paper make a choice of 3 phase induction motor.

• Speech Sciences
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• v.8 no.3
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• pp.201-208
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• 2001

The purpose of the study was to examine a method most pertinent to measure subglottic air pressure. Subglottic air pressure and loudness analyses were performed on vowels /a/, /i/ and consonant /p/ in 12 normal subjects using. Aerophone II voice function. The experimental contexts were, therefore, /i:pi:pi:/ and /a:pa:pa:/. The subjects produced the intervocalic /p/ in 4 different situations: 1) /i:pi:pi:/ with voiceless /p/, 2) /i:pi:pi:/ with voiced /p/, 3) /a:pa:pa:/ with voiceless /p/, and 4) /a:pa:pa:/ with voiced /p/. A t-test and a correlation analysis revealed the following results. First, when we measured subglottic air pressure by /i:pi:pi:/, voiceless /p/ was significantly different from voiced /p/. Second, when we measured subglottic air pressure by /a:pa:pa:/, voiceless /p/ was significantly different from voiced /p/. Therefore, it was concluded that voiceless /p/ produced more accurate subglottic air pressure and clinicians needed to have patients produce accurate /p/ when measuring subglottic air pressure using Aerophone II.

• BMB Reports
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• v.29 no.6
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• pp.555-563
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• 1996

A membrane-bound phosphatidylinositol 4-kinase (PI 4-kinase) was separated in a sucrose gradient and solubilized with 1% Triton X-100 from mouse brain. The enzyme was purified 2,952-fold by various chromatographic techniques including DEAE-cellulose, PI-Sepharose and Sephacryl S-200 gel filtration. The molecular weight of PI 4-kinase was approximately 76 kDa by gel filtration and 70.8 kDa by SDS-polyacrylamide gel electrophoresis. The purified enzyme exhibited specific activity of 11.2 nmol/min/mg protein and pi value of 4.7. Kinetic analysis of the PI 4-kinase indicated apparent $K_m$, values of 190 ${\mu}M$ and 120 ${\mu}M$ for phosphatidylinositol and ATP, respectively. The maximal activity of this purified enzyme was observed at pH 7.4 at an incubation temperature of $37^{\circ}C$. The enzyme activity was significantly activated by $Mg^{2+}$, $Mn^{2+}$ and $Fe^{2+}$, and inhibited severely by $Ca^{2+}$. PI 4-kinase was proved to be pure in its immunoblot test by polyclonal antibody prepared from immunized rabbit sera. By this test, we were able to detect the existence of the same type of PI 4-kinase from other mouse organ tissues, such as liver, heart, kidney and spleen. Furthermore, similar immunoblot analysis with the same antisera recognized the different epitopes of PI 4-kinase proteins from various organs of rabbit, chinese hamster and rat.

• Magazine of the Korean Society of Agricultural Engineers
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• v.16 no.4
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• pp.3572-3584
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• 1974

본 논문은 Thrower의 양곡성능에 관한 예측 방정식을 차원해석법을 적용하여 유도하는데 있다. Thrower의 능률에 큰 영향을 미친다고 생각되는 인자들간의 관계식들을 Buckingham의 $\pi$이론에 의하여 구하여 졌으며 본연구에 사용한 Thrower는 현재 농촌에서 사용되고 있는 자동탈곡기의 Thrower와 유사한 형으로 제작된 것이며 실험에 사용된 시료는 벼의 경우는 통일벼와 미네히까리 품종이고 보리 품종으로서는 나맥이며, Thrower의 Impeller 직경, 속도 및 투입량을 변화시켜 가면서 각 시료에 대하여 양곡높이를 측정하여 그 예측방정식을 유도하였다. 본 논문에서 얻은 결과는 다음과 같다. 1. Thrower의 양곡성능에 지대한 영향을 미친다고 생각되는 주요 인자들을 Thrower의 양정, impeller의 직경과 속도, 투입량, 곡립의 밀도, 중력가속도등이라 간주하여 Buckingham의 $\pi$이론을 적용하여 이들에 관한 $\pi$항들을 결정하였으며 그 결과는 다음과 같다. {{{{ pi 1= { D} over {H } , pi 2= { { DN}^{ { 1} over {2 } } } over { { g}^{ { 1} over { 2} } }, pi 3= { Q} over { { g}^{ { 1} over {2 } } { rho D}^{ { 2} over {6 } } }}}}} 2. 실험에 의하여 구해진 $\pi$항문의 관계식을 합성하여 Thrower의 양곡성능에 관한 시료별 예측방정식을 구하였으며 이 식들은 아래와 같다. 통일 : $\pi$1=0.1223($\pi$2)-1.1813$\times$($\pi$3)-0.1864 미네히까리 : $\pi$1=0.1185($\pi$2)-0.9663$\times$($\pi$3)-0.1575 나맥 : $\pi$1=0.1599($\pi$2)-1.2350$\times$($\pi$3)-0.1382 3. 현재 농촌에서 사용되고 있는 자동탈곡기체 부착된 Thrower의 회전수, Impeller의 직경 및 투입량은 각각 1,100rpm, 30cm 및 150gr/sec 정도로서 이와같은 수준의 변량들을 기초로 하여 본 논문에서 유도한 예측방정식을 적용하여 Thrower의 양정을 산출한 결과, 통일벼의 경우는 2.6m정도였고 미네히까리 품종과 나맥의 양정은 각각 2.4m, 2.9m 정도였다. 이와같은 사실로부터 1m내외의 양정만으로 되어있는 자동탈곡기 Thrower의 경우 제작비절감, 소요동력 감소를 기하고 아울러 과립의 손상을 줄이기 위해서는 설계 또는 작동조건의 변경을 통해 Thrower의 회전수를 현재 수준이하로 줄일 필요가 있다고 판단되었다.

• Korean Journal of Materials Research
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• v.5 no.8
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• pp.1040-1044
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• 1995

Magnetic garnet films of (YSmLuCa)$_3$(FeGe)$\_$5/O$\_$12/ have been grown by the liquid phase eqitaxy method on the substrate of non-magnetic garnet Gd$_3$Ga$\_$5/O$\_$12/. The variation of Sm ion concentration were varied 0.3, 0.4, 0.6, mole/formula unit respectively. The magnetic properties of the samples for the bubble magnetic materials, such as, line width ΔH of ferromagnetic resonance (FMR), magnetic saturation induction 4$\pi$Ms, wall mobility u$\_$w/ uniaxial magnetic anisotropy energy Ku, were measured and discussed the relations between these properties. The line width ΔH decreases with increasing 4$\pi$Ms, and with decreasing Sm concentration. The anisotropy energy Ku increases not only with increasing Sm ion concentration, but also increasing 4$\pi$Ms. The value of wall mobility u$\_$w/ increase with increasing 4$\pi$Ms and decreases with increasing Sm concentration. We define a physical constant Eι from the fact that the product of 4$\pi$Ms and ΔH is constant with dimension of energy density. The Eι is dependent only on Sm concentration.density. The Eι is dependent only on Sm concentration.

• BMB Reports
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• v.47 no.7
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• pp.361-368
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• 2014

Lipid components in biological membranes are essential for maintaining cellular function. Phosphoinositides, the phosphorylated derivatives of phosphatidylinositol (PI), regulate many critical cell processes involving membrane signaling, trafficking, and reorganization. Multiple metabolic pathways including phosphoinositide kinases and phosphatases and phospholipases tightly control spatio-temporal concentration of membrane phosphoinositides. Metabolizing enzymes responsible for PI 4,5-bisphosphate (PI(4,5)P2) production or degradation play a regulatory role in Toll-like receptor (TLR) signaling and trafficking. These enzymes include PI 4-phosphate 5-kinase, phosphatase and tensin homolog, PI 3-kinase, and phospholipase C. PI(4,5)P2 mediates the interaction with target cytosolic proteins to induce their membrane translocation, regulate vesicular trafficking, and serve as a precursor for other signaling lipids. TLR activation is important for the innate immune response and is implicated in diverse pathophysiological disorders. TLR signaling is controlled by specific interactions with distinct signaling and sorting adaptors. Importantly, TLR signaling machinery is differentially formed depending on a specific membrane compartment during signaling cascades. Although detailed mechanisms remain to be fully clarified, phosphoinositide metabolism is promising for a better understanding of such spatio-temporal regulation of TLR signaling and trafficking.

• Polymer(Korea)
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• v.39 no.1
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• pp.88-98
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• 2015

4-Amino-N-hexadecylbenzamide-graphene sheets (AHB-GSs), used in the preparation of the polyimide (PI) nanocomposite films, were synthesized by mixing a dispersion of graphite oxide with a solution of the ammonium salt of AHB. The atomic force microscope image of functionalized-GS on mica and a profile plot revealed the average thickness of AHB-GS to be ~3.21 nm. PI films were synthesized by reacting 4,4'-biphthalic anhydride and bis(4-aminophenyl) sulfide. PI nanocomposite films containing various contents of AHB-GS over the range of 0-10 wt% were synthesized using the solution intercalation method. The PI nanocomposite films under different thermal imidization temperatures, 250 and $350^{\circ}C$, were examined. The graphenes, for the most part, were well dispersed in the polymer matrix despite some agglomeration. However, micrometer-scale particles were not detected. The average thickness of the particles was <10 nm, as revealed from the transmission electron microscope images. Only a small amount of AHB-GS was required to improve the gas barrier, and electrical conductivity. In contrast, the glass transition and initial decomposition temperatures of the PI hybrid films continued to decrease with increasing content of AHB-GS up to 10 wt%. In general, the properties of the PI hybrid films heat treated at $350^{\circ}C$ were better than those of films heat treated at $250^{\circ}C$.

• Journal of Life Science
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• v.31 no.1
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• pp.1-9
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• 2021

Brown seaweeds have been shown to decrease blood glucose levels and improve insulin sensitivity previously. In this study, we investigated the effect of fucoidan, a complex polysaccharide derived from brown seaweeds, on glucose uptake to improve insulin resistance, and examined its mechanism of action in 3T3-L1 adipocytes. We observed that fucoidan significantly increased glucose uptake and it was related to an increased expression of plasma membrane-glucose transporter 4 (PM-GLUT4) in 3T3-L1 adipocytes. Fucoidan treatment increased the activation of phosphatidylinositol-3-kinase (PI3K) and the phosphorylation of insulin receptor substrate 1 (IRS1tyr) compared with that of the control cells. Fucoidan also promoted the phosphorylation of Akt and protein kinase C (PKC)-λ/ζ compared to that of the control cells. Moreover, fucoidan significantly upregulated acetyl-CoA-carboxylase (ACC) and adenosine monophosphate - activated protein kinase (AMPK) phosphorylation. As a result, translocation of GLUT4 was significantly enhanced in 3T3-L1 adipocytes, which significantly promoted glucose uptake via the PI3K/AMPK pathways. The elevation of glucose uptake by fucoidan was blocked by inhibitor of PI3K and inhibitor of AMPK in 3T3-L1 adipocytes. These findings indicate that fucoidan might ameliorate glucose uptake through GLUT4 translocation to the plasma membrane by activating the PI3K/Akt and AMPK pathways in 3T3-L1 adipocytes. Fucoidan is thought to be of high material value to diabetes treatments and functional foods.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1446-1451
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• 2013

The quality characteristics of pork patty prepared with chitosan containing preservatives during storage at $4^{\circ}C$ were investigated to improve its functional property and shelf life using food preservatives of natural origin (PI; 0.1% chitosan, 2.5% citric acid, 100 ppm grapefruit seed extract, 0.2% adipic acid, 0.1% Cinnamomum extract, D.W. 97.09% and PII; 0.1% chitosan, 2.5% citric acid, 100 ppm grapefruit seed extract, 0.2% adipic acid, 0.1% Lysimachia christinae Hance extract, D.W. 97.09%). The sensory qualities (taste, appearance, texture and overall acceptability) of patties preserved with PI (1, 3 and 5%) and PII (1, 3 and 5%) were also investigated. The taste and overall acceptability of patties prepared with 1% PI and 1% PII were higher than those of the control. The DPPH radical scavenging activities of the control, 1% PI and 1% PII treated patties were 13.54%, 21.78% and 14.07%, respectively. The total aerobes and coliform count of patties preserved with 1% PI were $10^4$ CFU/g and $10^2$ CFU/g, respectively, after 10 days storage at $4^{\circ}C$. Cooking loss from 1% PI and 1% PII treated patties was lower than that of from the control. Water holding capacity was the highest for 1% PI (65.84%), followed by 1% PII and then the control. The shelf life of patties prepared with 1% PI and 1% PII was extended by about 5 days relative to the control at $4^{\circ}C$.