• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2015년도 춘계학술대회
• /
• pp.943-946
• /
• 2015

탈수초화를 위해 쥐의 배아(임신 16일)의 슈반세포와 뉴런 세포가 척수신경절로 부터 in vitro 시스템에서 배양되었다. 항 유사분열제로 첨가된 분리 정제된 척수신경절 세포와 분리 정제된 슈반세포가 공동배양 배양되었고 배양되었고 수초화 과정이 구축되었다. 이렇게 형성된 공동 배양에 M. leprae-specific phenolic glycolipid-1 (PGL-1)을 처리하고 myelin basic protein (MBP)의 항체를 이용하여 탈수초화가 형성되었음을 확인하였다.

• 대한수학회보
• /
• 제52권6호
• /
• pp.2025-2034
• /
• 2015

In this paper, we characterize some PGL(2, q) by their orders and maximum element orders. We also prove that PSL(2, p) with $p{\geqslant}3$ a prime can be determined by their orders and maximum element orders. Moreover, we show that, in general, if $q=p^n$ with p a prime and n > 1, PGL(2, q) can not be uniquely determined by their orders and maximum element orders. Several known results are generalized.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제14권2호
• /
• pp.69-74
• /
• 2007

As the genome of B. mori is available in GenBank and the EST database of B. mori is expanding, identification of novel genes of B. mori was conceivable by datamining techniques and bioinformatics tools. In this study, we used the in silico cloning method to get the 6-Phosphogluconolactonase (6PGL) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and prokaryotic expression. The 6PGL cDNA comtains a 702 bp ORF. The deduced protein has 233 amino acid residues, with the predicted molecular weight of 25946. 72 Da, isoelectric point of 5.41, and contains conserved NagB domains. This gene has been registered in GenBank under the accession number EF198104.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2004년도 춘계학술대회 논문요약집
• /
• pp.218-218
• /
• 2004

자동화된 조립 생산분야에서 복잡하거나 정교한 작업을 수행하기 위해 Workcell 안에서 여러개의 디바이스들을 제어할 수 있는 지능적인 제어기를 개발할 필요성이 증대되고 있다. 새롭게 만들어지는 생산 장비의 각 디바이스에서 센서들과 엑츄에이터들을 동시성(Parallelism)과 실시간성(Real timeness)을 고려하여 어떻게 작업 지시를 프로그래밍하는가 하는 문제를 해결하여야 한다. 이를 위해 지금까지 대부분의 장비에서 사용하는 작업 교시 방법들은 특정한 동작을 나타내는 의미 명령어로 구성된 작업을 기술하거나, 사용자 인터페이스를 통해 장비업체가 일방적으로 정한 작업 지시를 입력하는 형태로서 개발되었다.(중략)

• 미생물학회지
• /
• 제46권1호
• /
• pp.96-103
• /
• 2010

단백질의 N-글리코실화는 대표적인 번역 후 변형 중의 하나로 진핵생물 뿐 아니라 원핵생물에서도 발견된다. N-글리코실화는 단백질 상의 N-글리코실 서열인 N-x-S/T 위치에 지질과 연결된 올리고당(lipid-linked oligosaccharide, LLO)으로부터 올리고당 전이효소(oligosaccharyltransferase, OTase) 활성에 의해 글리칸(glycan)이 전달되어 당단백질의 합성이 이루어진다. 본 연구에서는 OTase의 세포내 활성을 측정하기 위하여 5/6-carboxyltetramethylrhodamine (TAMRA)이 도입된 형광펩타이드 TAMRA-DA$\underline{N}$Y$\underline{T}$K-$NH_2$를 이용하였다. OTase활성 측정은 단일 서브유닛으로 효소의 활성을 갖는 운동핵 편모충류인 Leishmania major Stt3p와 병원성 미생물인 Campylobacter jejuni PglB를 진핵생물과 원핵생물의 모델 효소로 각각 사용하여 Saccharomyces cerevisiae와 C. jejuni 유래 LLO와 형광 펩타이드를 반응시켜 당-펩타이드를 합성하였다. 합성된 당-펩타이드를 미반응한 형광펩타이드와 분리 및 당-펩타이드의 정량 분석을 위하여 Tricine SDS-PAGE, ConA 렉틴 컬럼 및 fluorospectrophotometer, HPLC를 사용하였으며, 당-펩타이드 분석을 통해 각 방법의 장단점을 비교하였다. 비교 분석 결과 Tricine SDS-PAGE를 이용한 형광 이미지 분석과, 렉틴 컬럼을 통해 분리된 당-펩타이드의 fluorospectrophotometer 정량법에 비해, HPLC를 이용한 방법이 OTase에 의해 생성된 당-펩타이드를 분석하는데 더 정확하고 정량적인 값을 제시하는 것으로 확인되었다.

• Journal of Pharmaceutical Investigation
• /
• 제31권4호
• /
• pp.217-224
• /
• 2001

To develop a novel transdermal delivery system of loxoprofen (LP), a potent antiinflammatory and analgesic agent, the effects of various vehicles and penetration enhancers on the skin permeation of LP from solution formulations were investigated. The permeation rate of LP through excised guinea pig skin was measured using a side-by-side permeation system at $32^{\circ}C$. The solubilities of LP in various vehicles were determined by the equilibrium solubility method, and partition coefficients $(P_c)$ were determined. The solubility of LP increased in the rank order of water & isopropyl myristate (IPM) & glyceryl dicaprylate/dicaprate & propylene glycol dicaprylate/caprate & propylene glycol laurate (PGL) & polyethylene glycol 400 & diethylene glycol monoethyl ether (DGME) & ethanol. n-Octanol-water $P_c$ value was 15.5. Among pure vehicles tested, IPM and PGL showed highest fluxes of $89.9{\pm}5.0$ and $45.4{\pm}0.3\;{\mu}g/cm^2/hr$ from saturated solutions, respectively. However, it was not possible to demonstrate any correlation between the solubility of LP and its permeation rate, indicating the change in the barrier property of the skin and/or carrier mechanisms by vehicles tested. The addition of DGME to IPM or PGL markedly increased the solubility of LP, but the permeation rate did not decrease when the concentration of DGME in the cosolvent was increased upto 40%. The addition of linoleic acid (3%) in the cosolvent slightly increased the permeation rate, but others such as lauroyl sarcosine, fatty alcohols and fatty acids tested did not show enhancing effect. In conclusion, the DGME-IPM or DGME-PGL cosolvent system proved to be a good vehicle to enhance the skin permeation of LP.

• Rapid Communication in Photoscience
• /
• 제4권2호
• /
• pp.48-49
• /
• 2015

For myelination, Schwann cells and neuron cells from dorsal root ganglion (DRG) of rat embryos (E16) were cultured in vitro system. The purified DRG cells with anti-mitotic agents and purified Schwann cells were cocultured and then accomplished myelination processing. Treatment of M. leprae-specific phenolic glycolipid-1 (PGL-1) into this coculture system was performed and then accomplished demyelination. Therefore, we identified demyelination processing using antibody of myelin basic protein (MBP).

• Genomics & Informatics
• /
• 제4권4호
• /
• pp.182-187
• /
• 2006

6-Phosphogluconolactonase (6PGL) is one of the key enzymes in the ubiquitous pathways of central carbon metabolism, but bacterial 6PGL had been long known as a missing enzyme even after complete bacterial genome sequence information became available. Although recent experimental characterization suggests that there are two types of 6PGLs (DevB and YbhE), their phylogenetic distribution is severely biased. Here we present that proteins in COG group previously described as 3-oarboxymuconate cyclase (COG2706) are actually the YbhE-type 6PGLs, which are widely distributed in Proteobacteria and Fimicutes. This case exemplifies how erroneous functional description of a member in the reference database commonly used in transitive genome annotation cause systematic problem in the prediction of genes even with universal cellular functions.

• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2015년도 추계학술대회
• /
• pp.830-833
• /
• 2015

쥐의 배아(배아 16일)의 척수신경절에서 슈반세포와 뉴런 세포가 실험실 상에서 분리되었고 정제되었다. 정제된 척수신경절 세포는 유사분열 억제제를 첨가하여 배양되었고 분리 정제된 슈반세포와 공동배양 배양시켰다. 이 결과로 마침내 수초화 과정이 구축되었다. 이 수초화는 M. leprae-specific phenolic glycolipid-1 (PGL-1) 성분으로 처리하였고 그 결과 neurofilament의 단클론 항체를 이용하여 수초화와 탈수초화를 비교 및 분석하였다.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2001년도 ICCAS
• /
• pp.121.5-121
• /
• 2001

For the last decade, remarkable progress in the coating weight uniformity of hot dip galvanized product has been made to overcome the tightening quality constraints and produce cost-effective galvanized products. This progress results from research and development works for more efficient air knife, more accurate model of coating process, more precise measurement of coating weight and more efficient control logics. The activities for an efficient mathematical model to predict coating weight and several control logics which has been implemented on the No.1 CGL, No. 2 CGL, and PGL at KwangYang Steel Works are reviewed in this article.