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Cloning and Characterization of 6-Phosphogluconolactonase Gene in Silkworm Bombyx mori  

Yang, HuaJun (Institute of Life Sciences, Jiangsu University)
Chen, KePing (Institute of Life Sciences, Jiangsu University)
Yao, Qin (Institute of Life Sciences, Jiangsu University)
Guo, ZhongJian (Institute of Life Sciences, Jiangsu University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.14, no.2, 2007 , pp. 69-74 More about this Journal
Abstract
As the genome of B. mori is available in GenBank and the EST database of B. mori is expanding, identification of novel genes of B. mori was conceivable by datamining techniques and bioinformatics tools. In this study, we used the in silico cloning method to get the 6-Phosphogluconolactonase (6PGL) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and prokaryotic expression. The 6PGL cDNA comtains a 702 bp ORF. The deduced protein has 233 amino acid residues, with the predicted molecular weight of 25946. 72 Da, isoelectric point of 5.41, and contains conserved NagB domains. This gene has been registered in GenBank under the accession number EF198104.
Keywords
6PGL; Bombyx mori; Bioinformatics; RT-PCR; Prokaryotic expression;
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