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Effects of Artemisia argyi flavonoids on growth performance and immune function in broilers challenged with lipopolysaccharide

  • Yang, Shuo;Zhang, Jing;Jiang, Yang;Xu, Yuan Qing;Jin, Xiao;Yan, Su Mei;Shi, Bin Lin
    • Animal Bioscience
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    • v.34 no.7
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    • pp.1169-1180
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    • 2021
  • Objective: This research aimed to study the effects of Artemisia argyi flavonoids (AAF) supplemented in diets on the growth performance and immune function of broiler chickens challenged with lipopolysaccharide (LPS). Methods: A total of one hundred and ninety-two 1-d-old broiler chicks were assigned into 4 treatment groups, which were, respectively, fed a basal diet (control), fed a diet with 750 mg/kg AAF, fed a basal diet, and challenged with LPS, fed a diet with 750 mg/kg AAF, and challenged with LPS. Each treatment had six pens with 8 chicks per pen. On days 14, 16, 18, 20 (stress phase I) and 28, 30, 32, 34 (stress phase II), broilers were injected with LPS (500 ㎍/kg body weight) or an equivalent amount of saline. Results: The results demonstrated that dietary AAF significantly improved the body weight (d 21) and alleviated the decrease of average daily gain in broilers challenged with LPS on d 21 and d 35 (p<0.05). Dietary AAF increased bursa fabricius index, and dramatically attenuated the elevation of spleen index caused by LPS on d 35 (p<0.05). Furthermore, serum interleukin-6 (IL-6) concentration decreased with AAF supplementation on d 21 (p<0.05). Diet treatment and LPS challenge exhibited a significant interaction for the concentration of IL-1β (d 21) and IL-6 (d 35) in serum (p<0.05). Additionally, AAF supplementation mitigated the increase of IL-1β, IL-6 in liver and spleen induced by LPS on d 21 and 35 (p<0.05). This study also showed that AAF supplementation significantly reduced the expression of IL-1β (d 21) and nuclear transcription factor kappa-B p65 (d 21 and 35) in liver (p<0.05), and dietary AAF and LPS treatment exhibited significant interaction for the gene expression of IL-6 (d 21), toll like receptor 4 (d 35) and myeloid differentiation factor 88 (d 35) in spleen (p<0.05). Conclusion: In conclusion, AAF could be used as a potential natural immunomodulator to improve growth performance and alleviate immune stress in broilers challenged with LPS.

The Effect of Applying Various Tools to the Stiffness and Muscle Tone of Hamstring Muscles (다양한 도구의 적용이 뒤넙다리근의 뻣뻣함과 근 긴장도에 미치는 영향)

  • Hwang, Sunghyun;Kim, Taeho
    • Journal of The Korean Society of Integrative Medicine
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    • v.8 no.4
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    • pp.223-230
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    • 2020
  • Purpose : The purpose of this study was to examine the effects of tools (i., extracorporeal shock wave therapy, massage gun, and foam roller) on range of motion, muscle tone and pain threshold among patients with hamstring stiffness. Methods : Fourteen participants with hamstrings stiffness were recruited. Interventions were performed 6 times, and each session was for 30 seconds using the three tools. The range of motion, muscle tone, and pain threshold were measured. The order of the use of the three tools was randomly determined. The foam roller was made to move from the bottom of the hip crease to the upper part of the back of the hamstring. Additionally, velocity 5 vibration stimulation was performed on the hamstring using a massage gun. Moreover, vibration stimulation was performed on the hamstring with extracorporeal shock wave therapy 5 minutes, 5 Hz, and 1,500 strokes. The flexibility of the posterior thigh muscle was based on maintaining the knee and hip joints in a 90 ° bend in the supine position. The joint angle of the knee was measured, when the knee was actively extended, at the maximum point where the posterior thigh muscle was stretched. The elasticity of the posterior thigh muscle was measured while the subject was prone and in a relaxed state without any force. Measurements were made at the muscle abdominal area of the semitendinosus muscle of the posterior femur, and the area to be measured was marked with a pen. The measurement of the tenderness threshold of the posterior femur was measured using a tenderness meter(Commander Algometer, J-Tech, USA). The force value at the point at which the pressure sensation change to pain was measured after applying vertical pressure to the posterior femur muscle, which was the halfway point between the ischial tuberosity and the popliteal surface of the subject lying on their stomach. Results : The extracorporeal shock wave therapy increased stiffness and, muscle tone, and caused changes in the pain threshold, whereas the other two tools had no effect on these indices. Conclusion : Extracorporeal shock wave therapy has important effects on range of motion and muscle stiffness and can be used in warmup protocols.

A Study about Gaya Architecture with House-Shaped Earthenware (집모양토기를 통한 가야 건축 연구)

  • Han, Wook
    • Korean Journal of Heritage: History & Science
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    • v.54 no.1
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    • pp.32-51
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    • 2021
  • Architectural evidence of Gaya is insignificant than any other area. In this situation, house-shaped earthenware is particularly helpful in the study of Gaya architecture. However, house-shaped earthenware is not a building, it has just the shape of buildings. So, we have to find out architectural evidence from that. In this paper, I tried to estimate the architectural characteristics of Gaya through house-shaped earthenware in detail. I also consulted historical records, archeological results, and other scholars' papers. The results are as follows. First, house-shaped earthenware can be classified into three types according to its shape, and each type has distinct architectural structural characteristics. Second, house-shaped earthenware generally has a gambrel roof, and the characteristic of architectural design with the gable side of the gambrel roof as a front is the biggest feature of Gaya architecture. Third, various types of buildings existed according to their functions in Gaya architecture, and house-shaped earthenware was showed buildings of ancient storage, pens, and residences, which are symbols of the richness among various types of buildings.

Monitoring and Distribution of Mosquitoes in the Downtown Areas and Migratory Bird Habitats in the Jeonbuk Region, 2021 (2021년 전북지역 도심과 철새서식처의 모기 감시와 분포)

  • Park, Ho;Kwon, Pil-Seung
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.2
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    • pp.142-148
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    • 2022
  • This survey was performed to monitor the spread of specific mosquito-borne pathogens at Jeonbuk. The frequency of occurrence of mosquito borne pathogens including Japanese encephalitis virus, West Nile virus, Zika virus, and yellow fever virus was assessed by collecting mosquitoes twice a month from March to December 2021 from various areas in Jeonbuk. A total of 15,975 mosquitoes from 15 species and 7 genera were collected. The highest number of 9,116 mosquitoes (trap index: TI, 506.4) were collected in the Wanju cattle pen, followed by the habitat for migratory birds and the downtown area in Jeonju. In the Gunsan habitat for migratory birds, 3,217 mosquitoes (TI, 178.7) were collected in the reed fields, 356 (TI, 19.7) in the men's toilets, and 1,948 (TI, 108.2) in the women's toilets. In Jeonju, 677 mosquitoes (TI, 37.6) were collected in the Deokjin park, 358 (TI, 19.8) in the Deokjin-gu office, and 303 (TI, 16.8) at the Jeonbuk National University. The largest population of mosquitoes was collected in the men's toilets in Gunsan and the Deokjin Park in downtown Jeonju. The results of the RT-PCR confirmation to determine the pathogen infection of the collected mosquitoes were all negative. These results provide a basis for tackling integrated mosquito-borne diseases in the Jeonbuk region.

Effect of Perch Material Type on Performance, Blood Characteristics, and Feather Score of Layer Pullets on Floor during Growth Period (산란계 평사 사육시 육성기 홰 재질에 따른 생산성, 혈액성상, 깃털 손상도에 미치는 영향)

  • Kim, Hee-Jin;Son, Jiseon;Hong, Eui-Chul;Kang, Hwan Ku
    • Korean Journal of Organic Agriculture
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    • v.29 no.4
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    • pp.589-600
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    • 2021
  • The present study investigated the effect of perch material type (wood and steel) on performance, blood characteristics, and feather pecking. A total of 1,700, one-day-old female Hy-Line brown chicks were evenly assigned to two treatments (wood and steel). The body weight and uniformity of birds were investigated over a 10-week experimental period. Blood characteristics and feather pecking was conducted at week 10. The wood perch treatment (WP) and steel perch treatment (SP) did not differ in their body weight, uniformity, and serum biochemical profile (except inorganic phosphorus) of pullets. Inorganic phosphorus on serum in WP significantly higher than that in SP (P<0.05). Blood corpuscle composition (leukocyte, erythrocyte, and Heterophil / Lymphocyte ratio) of pullets did not show any significant differences among treatments at week 10. Serum corticosterone and feather score that is stress and welfare indices were not affected by the type of perch material. In conclusion, our results suggest that performance, blood characteristics, and feather pecking of pullets were not affected by the perch material type. The results of this study can serve as basic data for investigating the effects of perch type on layer pullets.

Effect of feeding a diet comprised of various corn silages inclusion with peanut vine or wheat straw on performance, digestion, serum parameters and meat nutrients in finishing beef cattle

  • Zhang, Hongrui;Zhang, Liyang;Xue, Xiao;Zhang, Xiaoxia;Wang, Hongyi;Gao, Tengyun;Phillips, Clive
    • Animal Bioscience
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    • v.35 no.1
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    • pp.29-38
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    • 2022
  • Objective: The objective of this study was to compare the feeding value, meat nutrients and associative effects of a diet comprised of various corn silages inclusion with peanut vine or wheat straw in finishing beef cattle. Methods: One hundred and eighty Simmental crossbred beef steers were blocked and assigned to the follow treatments: i) whole plant corn silage-based diet (control, WPCS), ii) mixed forages-based diet (replacing a portion of corn silage with wheat straw, WPCSW), iii) corn stalklage-based diet (CS), and iv) sweet corn stalklage-based diet (SCS). Each group consisted of 5 repeated pens with 9 steers/pen. The diets were formulated to be isonitrogenous and isoenergetic with same forage to concentrate ratio. Experimental diets were fed for 90 d. Results: The effective ruminal degradability of dry matter and crude protein were highest for WPCS diet (p<0.05), for neutral detergent fiber was highest in SCS diet (p<0.05). The average daily gain was greater for cattle offered the WPCS diet, intermediate with WPCSW and SCS and lowest with CS (p<0.001). The concentration of non-esterified fatty acid in serum was higher for steers fed with CS and SCS diets than those offered WPCS and WPCSW steers (p<0.001). The treatments did not affect the general nutritional contents and amino acids composition of Longissimus dorsi of steers (p>0.05). Conclusion: The corn silage-based diet exhibited the highest feeding value. The sweet corn stalklage and wheat straw as an alternative to corn silage offered to beef cattle had limited influence on feeding value and meat nutrients. However, the value of a corn stalklage-based diet was relatively poor. To sum up, when the high quality forage resources, such as corn silage, are in short supply, or the growth rate of beef cattle decreases in the later finishing period, the sweet stalklage and wheat straw could be used as a cheaper alternative in feedlot cattle diet without sharp reducing economic benefits.

Effects of Dietary Exogenous Hydrophilic Emulsifier Supplementation on Growth Performance and Carcass Traits in Broilers

  • Choi, Hyo Sim;Hong, Jin Su;Lee, Geon Il;Kim, Yoo Yong
    • Korean Journal of Poultry Science
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    • v.49 no.2
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    • pp.61-67
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    • 2022
  • The effects of dietary exogenous hydrophilic emulsifiers on the growth, nutrient digestibility, and carcass characteristics of broilers were evaluated. A total of 200 one-day-old broilers (Ross 308) were allotted to one of four treatment groups in a randomized complete block design in five replicates with 10 birds per pen during a 5-week growth experimental period. Birds were fed a corn-soybean meal-based diet with or without the addition of 0.025, 0.050, or 0.075% exogenous hydrophilic emulsifiers. The diets contained 3,025 and 3,075 metabolizable energy/kg for Phases 1 and 2, respectively. For each phase and the overall experimental period, body weight gain (linear, P<0.05) and feed conversion ratio (linear, P<0.05) improved in proportion to the dietary exogenous hydrophilic emulsifier level, while the average daily feed intake was not affected by dietary treatment. Improvement in growth performance by dietary treatments was observed during the last two weeks rather than the first three weeks of the growth phase. In carcass traits, abdominal fat content increased as dietary exogenous hydrophilic emulsifier level increased (linear, P<0.05), whereas dietary emulsifier level did not affect the relative weight of the liver, breast, and leg muscles. In conclusion, addition of dietary exogenous hydrophilic emulsifiers from 0 to 0.075% in broiler diets improved the growth rate and feed efficiency of broilers without any deleterious effects on nutrient digestibility, although a corn-soybean meal-based diet had less energy content (3,025 and 3,075 metabolizable energy/kg) for 0-3 weeks and 3-5 weeks, respectively.

Detecting of Proximal Caries in Primary Molars using Pen-type QLF Device (펜-타입 QLF 장비의 임상적 유구치 인접면 우식 탐지 성능)

  • Cho, Hyejin;Kim, Hyuntae;Song, Ji-Soo;Shin, Teo Jeon;Kim, Jung-Wook;Jang, Ki-Taeg;Kim, Young-Jae
    • Journal of the korean academy of Pediatric Dentistry
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    • v.48 no.4
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    • pp.405-413
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    • 2021
  • The purpose of this in vivo study was to assess the clinical screening performance of a quantitative light-induced fluorescence (QLF) device in detecting proximal caries in primary molars. Fluorescence loss, red autofluorescence and a simplified QLF score for proximal caries (QS-proximal) were evaluated for their validity in detecting proximal caries in primary molars compared to bitewing radiography. Three hundred and forty-four primary molar surfaces were included in the study. Carious lesions were scored according to lesion severity assessed by visual-tactile and radiographic examinations. The QLF images were analyzed for two quantitative parameters, fluorescence loss and red autofluorescence, as well as for QS-proximal. For both quantitative parameters and QS-proximal, the sensitivity, specificity and area under receiver operating curve (AUROC) were calculated as a function of the radiographic scoring index at enamel and dentin caries levels. Both quantitative parameters showed fair AUROC values for detecting dentine level caries (△F = 0.794, △R = 0.750). QS-proximal showed higher AUROC values (0.757 - 0.769) than that of visual-tactile scores (0.653) in detecting dentine level caries. The QLF device showed fair screening performance in detecting proximal caries in primary molars compared to bitewing radiography.

Effects of light intensity on growth performance, blood components, carcass characteristics, and welfare of broilers

  • Hee-Jin, Kim;Jiseon, Son;Hyun-Soo, Kim;Eui-Chul, Hong;Ji-Hyuk, Kim
    • Journal of Animal Science and Technology
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    • v.64 no.5
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    • pp.985-996
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    • 2022
  • This study investigated the effects of light intensity on growth performance, blood components, eye condition, and carcass characteristics of broiler chickens. Three hundred and fifty-two 1-day-old male chicks were assigned to one of four treatments (four repetitions per treatment, 22 birds per repetition) and reared in a floor pen for 5 weeks. From the second week, chicks were reared under four different levels of light intensity (5, 20, 35, and 50 lx) and the lighting duration was maintained at 18-hours light : 6-hours dark (18L : 6D). The feed intake and body weight were measured weekly. At 35 days of age, 12 birds per treatment were randomly selected for blood sampling, eye measurement, and carcass analysis. There were no significant differences in body weight gain, feed intake, and feed conversion ratio among treatments. Triglyceride levels in the serum were significantly higher in the 5 lx treatment, and creatinine was significantly lower in the 5 lx treatment (p < 0.05). The heterophil : lymphocyte ratios decreased significantly as light intensity increased (p < 0.05); however, other blood cell compositions were not affected by light intensity. Interleukin-6 content was significantly higher in the 5 lx treatment than in other treatments (p < 0.05), but the content of tumor necrosis factor-α was not significantly different among treatments. Serum corticosterone concentration was significantly higher at 5 lx than at 20, 35, and 50 lx (p < 0.05). The corneal diameter was the highest in 5 lx treatment (p < 0.05), and tended to increase as the light intensity decreased. Other eye parameters were not significantly different among treatments, but displayed a tendency to increase as the light intensity decreased. Carcass yield and part yields were not affected by light intensity. Meat quality parameters (pH, color, cooking loss, and water-holding capacity) did not show significant difference among the treatments. The results indicate that a light intensity of 5 lx may increase physiological stress or have a negative effect on broiler welfare, even if the performance and carcass characteristics are not affected. Therefore, a light intensity of 20 lx or above is recommended considering both the growth performance and welfare of broilers.

Blend of essential oils can reduce diarrheal disorders and improve liver antioxidant status in weaning piglets

  • Maiara Ananda, Grando;Vanessa, Costa;Jansller Luiz, Genova;Paulo Evaristo, Rupolo;Liliana Bury, de Azevedo;Leandro Batista, Costa;Silvana Teixeira, Carvalho;Thiago Pereira, Ribeiro;Daniel Pigatto, Monteiro;Paulo Levi, de Oliveira Carvalho
    • Animal Bioscience
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    • v.36 no.1
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    • pp.119-131
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    • 2023
  • Objective: This study was to assess the effects of different doses of an essential oil blend (EOB) on growth performance, diarrhea occurrence (DO), hematological and blood biochemical profile, intestinal morphometry, morphology and microbiology, relative weight and length of organs, digestive content pH, and liver antioxidant status in weaning piglets. Methods: A total of 135 barrows (7.09±0.29 kg body weight) were allotted randomly in a randomized complete block design based on body weight with nine replications and three animals per pen. Dietary treatments were a negative control (NC): basal diet; positive control (PC): NC plus 125 mg performance-enhancing antibiotic (enramycin 8%)/kg diet; NC plus 100 mg EOB/kg diet (EO100); NC plus 200 mg EOB/kg diet (EO200); and NC plus 400 mg EOB/kg diet (EO400). Diarrhea occurrence was monitored daily, and performance at the end of each phase. Results: Gain to feed ratio was greater (p<0.05) in starter II pigs fed EO400 and EO200 than in those fed EO100. Pigs fed EO400 had lower (p<0.05) DO than those fed NC and EO100 in the total period. Pre-starter II pigs fed NC had (p<0.05) lower serum total protein and plasma protein than pigs fed PC. Pigs fed EO100 showed smaller (p<0.05) mean corpuscular volume (MCV) than pigs fed EO400. Starter II pigs fed EO400 had (p<0.05) greater MCV and lower mean corpuscular hemoglobin and erythrocytes than those fed EO100. There was a greater concentration (p<0.05) of band cells for PC, similar to EO400 and EO200. Performance-enhancing antibiotic and EOB to diets increased (p<0.05) liver superoxide dismutase activity. Conclusion: Adding 200 and 400 mg EOB/kg diet decreased DO and was advantageous to hematological and blood biochemical profile and liver antioxidant status without being detrimental to growth performance and gastrointestinal health in nursery pigs.