• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.515-519
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• 2004

Listeria spp. were isolated from a total of 402 naturally contaminated domestic ready-to-eat (RTE) vegetable samples by the conventional Food and Drug Administration protocol and confinned by API-Listeria kit. Also, the susceptibility to 12 antibiotics, polymerase chain reaction (PCR) assay for virulence gene of pathogenic Listeria monocytogenes isolates, and in vitro virulence assay using myeloma and hybridoma cells from murine and human sources were tested. Among the samples, 17 samples (4.2％) were found to be contaminated with Listeria species. Among the 17 strains of Listeria spp. isolates, only 2 strains (11.8％) of L. monocytogenes and 15 strains (88.2％) of L. innocua were identified. Antibiotic susceptibility test showed that the Listeria spp. isolates were very susceptible to the antibiotics tested, except for nalidixic acid. Among 17 strains of Listeria spp., PCR analysis showed that 2 strains of L. monocytogenes isolates proved to have a virulence hly gene, but none of L. innocua had the hly gene. Also, hybridoma Ped-2E9 cells assay showed that only L. monocytogenes isolates killed approximately 95-99％ hybridoma cells after 6 h, but L. innocua isolates had about 0-5％ lethal effect. These results indicate that PCR assay with hly primer or hybridoma Ped-2E9 cells assay could be used as a good monitoring tool or in vitro virulence test for L. monocytogenes.

• Journal of the Korean Applied Science and Technology
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• v.13 no.3
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• pp.83-94
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• 1996

EMA-co-DAMA were synthesized from 2-diethylaminoethyl metacrylate and ethylhexyl metacrylate in acrylmonomer. To facilitate water emulsification, acrylic copolymer was cationized by acetic acid to produce acetated acrylic copolymer. The structures of the synthesized copolymer and acetated copolymers were confirmed by IR, NMR, and molecular weight was measure by GPC, and C.H.N elemental analysis. Acetated acrylic copolymers were perfectly emulsified in water and showed increased emulsion stability. Polymer dispersion for cement modifier[(PDCM-PED) water proof agent of cement for concrete in building construction] was prepared by blending of the guaternized acrylic copolymer syndisized above, sodium silicate, sodium gluconate and oleic acid emulsion. The result with prepared polymer dispersion of cement modifier was examined, and it was found that excellent waterprooffing effect ; Water permeability ratio is 0.50 under the water pressure of $100g/cm^2$ and 0.60 under $3kg/cm^2$, and water absorption ratio is $0.42{\sim}0.50$ and $1.0{\sim}1.02$ compressive strength ratio at mixed of water/PDCM-PED is 50 times.

• Journal of Korean Neurosurgical Society
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• v.55 no.1
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• pp.32-35
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• 2014

The pipeline embolization device (PED) is a new endovascular device for treatment of complex, fusiform and wide-neck intracranial aneurysms. The main mechanism of this stent is to divert the flow in the parent artery with reduction of inflow in the aneurysm leading to thrombosis. We treated a 40-year-old woman who had left facial pain and orbit discomfort. Angiography showed a giant fusiform aneurysm located in the cavernous segment of the left internal carotid artery. A PED was successfully deployed across the aneurysm. The procedure and post-procedural course were uneventful. After 3 months, angiography showed complete obliteration of the aneurysm with good patency of the branching vessels originating from the deployed segment. The patient's symptoms improved completely without complications.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.9A
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• pp.714-721
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• 2005

In this parer, we analyze the preamble model in the OFDMA/TDD(OFDM-FDMA/Time Division Duplexing). Besides, under AWGN, ITU-R M.1225 Ped-B and Veh-A channel environments, we analyze capabilities of symbol timing & carrier frequency offset and performance of cell searching capabilities applied to OFDM/TDD system through computer simulation. The performance using Detection Probability, False Alarm Probability, Missing Probability, Mean Acquisition Time and MSE(Mean Square Error) is analyzed. Especially, in the case of symbol timing offset estimation, the preamble structure and its algorithm with enhanced performance are proposed and then compared with existing ones.

• KSBB Journal
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• v.26 no.1
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• pp.62-68
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• 2011

To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-${\beta}$ activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.5
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• pp.25-30
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• 2012

It is important to detect dangerous objects which are intentionally abandoned in public places. Nowadays visual surveillance system is required to enhance the performance in two ways : high resolution and wireless linking ability. In this study the design of visual surveillance system is newly proposed to detect abandoned objects for social security purpose based on wireless high resolution image transmission technology. Also, to enhance PED, PAT performance, the tracking algorithm is included in the previous visual surveillance software scheme. By implementing proposed design scheme on the real wireless high resolution image transmission system, the effectiveness of the overall system is shown with the transmission performance of 4.0 Gbps speed.

• Korean Journal of Food Science and Technology
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• v.27 no.2
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• pp.216-220
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• 1995

In order to investigate the possible use of vegetable kimchi stuff as natural preservatives for kimchi, the methanol extracts of 15 kinds of vegetable kimchi stuff were solvent-fractionated and fractions were tested for antimicrobial activities against Leuconostoc mesenteroids, Pediococcus cerevisiae, Lactobacillus plantarum and Saccharomyces cerevisiae. The neutral fractions of the extracts of garlic and leek showed strong antimicrobial activities. The extract of leek showed particularly strong antimicrobial activities against Ped. cerevisiae and L. plantarum that were known to be main microorganism of fermentation and acidification of kimchi. The results suggest the possible use of the leek extract as natural preservatives for kimchi.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.403-411
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• 2005

The relationship between plasmid (~9.5 kb) and pediocin PA-1 in P. acidilactici K10 was confirmed by plasmid curing. The pediocin operon of P. acidilactici K10 was amplified by PCR (polymerase chain reaction), and the nucleotide sequence was analyzed. The sequence of the pediocin operon of P. acidilactici K10 was similar to those of P. acidilactici strains producing pediocin PA-1/ AcH. For the expression of pediocin PA-1 in E. coli, a pQEPED (pQE-30 Xa::mature pedA) was constructed. His-tagged recombinant pediocin PA-1 (-6.5 kDa) was translated by cell-free in vitro transcription and translation using pQEPED as a DNA template. Theresult of slot blotting assay showed that transcription of recombinant pedA in E. coli M15 was induced by the addition of isopropyl-$\beta$-D-thiogalactopyranoside (IPTG) at the final concentration of 1 mM. Although the recombinant pediocin PA-1 inhibited the growth of E. coli, it was expressed in the host strain and purified by nickel-nitrilotriacetic acid (Ni-NTA) metal-affinity chromatography under denaturing condition. This is the first report for the production and one-step purification of biologically active recombinant pediocin PA-1 in E. coli.

• Bulletin of the Korean Chemical Society
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• v.6 no.2
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• pp.68-73
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• 1985

Normal modes of solids HF, HCl and polyethylene having the exciting spectrometric phenomena have been evaluated by taking the lowest temperature phase of these species in the solid. The solids HF and HCl have the same space group as C$_{2}{\nu}$, and polyethylene has a space group with D$_{2h}$. The normal modes were obtained by the valence force field with modified force constants and a quantitative description of the normal mode is adjusted by the potential energy distribution (PED). From the PED, the most fittable force constants are also obtained. We have intended to calculate the normal modes by using the smallest size of the model and the simple computational process. To remove the edge effects being occurred in constructing the single cluster model, different from the boundary condition being generally used up to now, the idea of pseudolattice method being successfully applied to MO calculations of solid was extended to normal mode analysis in order to give the same environment for all moecules in a chosen cluster. By using the above valence force field and boundary condition, we obtain the assigned frequencies and compare those results with the results obtained by others.

• Korean Journal of Veterinary Research
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• v.61 no.3
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• pp.23.1-23.8
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• 2021

Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were successfully propagated in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene revealed that all 3 isolates belong to genogroup 2a, which is closely related to other prevalent Asian strains. Amino acid sequence comparisons revealed 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013-2015, suggesting that field PED viruses (PEDVs) evolve continuously. Experiments in animals demonstrated that antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log₂) of neutralizing antibody against the homologous strain, and showed a relatively lower level of neutralizing antibody against the field isolates. This finding would be helpful in choosing a PEDV strain for vaccine development.