• Journal of the Korean Applied Science and Technology
• /
• v.33 no.4
• /
• pp.686-692
• /
• 2016

This study was done to investigate the antidiabetic effect of ethanol extract from Platycodon grandiflorum root in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose 45mg/kg.b.w. dissolved in citrate buffer(pH4.5). The ethanol extract of Platycodon grandiflorum root was orally administrated once a day for 7 days. The contents of serum glucose, triglyceride(TG) and total cholesterol were significantly decreased(p<0.05) in Platycodon grandiflorumt root treated group compared to the those of STZ-control group. Also the contents of hepatic glycogen and HDL-cholesterol, the activities of glucose-phosphate dehydrogenase(G-6-PDH) and glucokinase(GK) were significamtly increased (p<0.05). These results indicated that ethanol extract of Platycodon grandiflorum root would have antidiabetic effect in STZ-induced diabetic rats.

• Nutritional Sciences
• /
• v.3 no.1
• /
• pp.25-30
• /
• 2000

To study the effect of prosomillet (Panicum milaceum) on lipid metabolism, male Sprague-Dawley rats weighing 190$\pm$8g were fed six experimental diets for four weeks. The six diets based on AIN-76 composition consisted of one cholesterol-free(normal) and five 1%(w/w) cholesterol diets, i.e. control, two diets containing additional 0.3 and 0.6%(w/w) methanol extracts of prosomillet and another two diets containing 15 and 30% (w/w) prosomillet powder. There was no difference in weight gains between the groups but relative liver weights increased under the cholestrol diets. Plasma levels of total cholesterol and triglyceride(TG) decreased by 23-27% and by 37-52%, respectively, in the four prosomillet diet groups compared to those of the normal and control groups. Whereas in the liver, only TG levels decreased in the prosomillet diet groups. Fecal excretions of bile acid and cholesterol significantly with methanol extracts of prosomillet. There was a significant increase in the activity of hepatic microsomal cholesterol 7$\alpha$-hydroxylase when feeding 1% cholesterol but prosomillet in the diet, either as in the form of powder or methanol extract, appeared to have only slight additional effects, namely increases in enzyme activity. The activity of liver cytosolic glucose-6-phophate dehydrogenase (G6PDH) tended to be reduced with high cholesterol diets and dropped markedly by 15% using additional prosomillet powder. Those of the liver cytoxolic malic enzyme had a similar tendency to those of G6PDH. The results indicate that certain active components in prosmillet other than fiber have the potential to exert hypolipidemic effects via regulating cholesterol excretions and lipogenesis.

• Journal of the Korean Applied Science and Technology
• /
• v.36 no.3
• /
• pp.898-904
• /
• 2019

This study was carried to investigate the antidiabetic effect of ethanol extract of Astragali Radix(A.R) in Streptozotocin(STZ) induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45mg/kg dissolved in citrate buffer. The ethanol extract of A. R was orally administrated once a day for 7 days at a dose of 1,000mg/kg. The contents of serum glucose, triglyceride(TG), total cholesterol were significantly decreased in A.R treated group compared to the those of STZ-control group. The content of hepatic glycogen and activities of glucokinase(GK) and glucose-6-phosphate dehydrogenase(G-6-PDH) were significantly increased, and activity of glucose-6-phoshatase(G-6-Pase) was significantly decreased in A.R treated group compared to the those of STZ-control group, These results indicated that ethanol extract of A.R would have antidiabetic effect in STZ-induced diabetic rats.

• Korean Journal of Exercise Nutrition
• /
• v.24 no.2
• /
• pp.1-5
• /
• 2020

[Purpose] Lactate has several beneficial roles as an energy resource and in metabolism. However, studies on the effects of oral administration of lactate on fat metabolism and glycogen synthesis are limited. Therefore, the purpose of the present study was to investigate how oral administration of lactate affects fat metabolism and glycogen synthesis factors at specific times (0, 30, 60, 120 min) after intake. [Methods] Male Sprague Dawley (SD) rats (n = 24) were divided into four groups as follows: the control group (0 min) was sacrificed immediately after oral lactate administration; the test groups were administered lactate (2 g/kg) and sacrificed after 30, 60, and 120 min. Skeletal muscle and liver mRNA expression of GLUT4, FAT/CD36, PDH, CS, PC and GYS2 was assessed using reverse transcription-polymerase chain reaction. [Results] GLUT4 and FAT/CD36 expression was significantly increased in skeletal muscle 120 min after lactate administration. PDH expression in skeletal muscle was altered at 30 and 120 min after lactate consumption, but was not significantly different compared to the control. CS, PC and GYS2 expression in liver was increased 60 min after lactate administration. [Conclusion] Our results indicate that exogenous lactate administration increases GLUT4 and FAT/CD36 expression in the muscle as well as glycogen synthase factors (PC, GYS2) in the liver after 60 min. Therefore, lactate supplementation may increase fat utilization as well as induce positive effects on glycogen synthesis in athletes.

• Journal of Nutrition and Health
• /
• v.17 no.4
• /
• pp.290-296
• /
• 1984

In order to evaluate the effect of fish oil on lipogenesis, activities of glucose-6-phosphate dehydrogenase ( G6PDH ) and malic enzyme (ME) were measured in liver of rats fed mackered oil(MO) or eel oil (EO) for 10 to 14 days, at the various levels of 0 to 10% (w/w ). In addition to two kinds of fish oil, soybean oil (SO), lard (L), and beef tallow (BT) were fed to the different groups of rats. When fish oil was below 10%(w/w ), soybean oil, lard, or beef tallow was mixed with fish oil to maintain constant 10% (w/w) fat level. Three days of feeding MO brought a marked decrease$({\sim}{50}%)$ both in G6PDH and ME activity, the former of which maintained during 13 days of feeding. L group had highest levels of both enzymes. G6PDH activity of MO was lower than SO, but ME activity was not different between MO and SO. G6PDH activity was decreased with increasing content of fish oil (MO, EO), starting at the 2%(w/w) level of fish oil, when L or BT was used as filler oil. But ME activity was significantly reduced when fish oil content was at least 5%(w/w). Difference between the effects shown by two kinds of fish oil and animal species were also found. The present study suggests that fish oil can suppress hepatic lipogenesis by reducing activities of lipogenic enzymes with the same or higher degree than vegetable oil can exert.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.5
• /
• pp.542-547
• /
• 2008

The anti-diabetic effects of water extracts (WE) from medicinal herbs on hepatic glucose-regulating enzymes, such as glucokinase (GCK), pyruvate dehydrogenase (PDH), acetyl-CoA carboxylase (ACC) and ${\alpha}$-glucosidase, were studied using the cytosol fraction in liver and mitochondia fraction in heart of a type II diabetic animal (GK rat, Goto-Kakizaki). The free radical scavenging activity of water extracts by DPPH method was also tested. We found that free radical scavenging activity was strong in Corni fructu (CF), Mokdan Bark (MDB), Chenhwabon (CHB) and Sanyack (SY), while that of Backbocreng (BBR), Shuckgihwang (SGH) and Taecsa (TS) was lower. For GCK activity in cytosol of liver, CF and CHB had a more effective activity than other extracts. PDH activity in mitochondria fraction of heart was higher in all of the extracts, expect for the TS extract, than in the control. ACC activity in cytosol fraction of liver was significantly higher in the CF, CHB, SGH, TS and SY extracts than in the control. CF, BBR and MDB led to a decrease in the ${\alpha}$-glucosidase activity. Therefore, these results suggest that all of the extracts may be used as functional material in the development as anti-diabetic functional food and medicine.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.11a
• /
• pp.220-220
• /
• 2005

• Journal of the Korean Applied Science and Technology
• /
• v.29 no.3
• /
• pp.412-420
• /
• 2012

This study was carried out to investigate the antidiabetic effect of Lonicerae japonica Thunb(LJ) in the streptozotocin(STZ)-induced diabetic rats. The effective fractions were prepared as a form of organic solvents of hexane, chloroform, ethylacetate, butanol, water fractions prepared from the ethanol extract of LJ. The content of serum glucose and the activities of glucose-6-phosphatase(G-6-Pase) in the hexane and water fractions treated rats were significantly decreased compared to those of the STZ control group. Whereas the activity of hepatic glucose-6-phosphate dehydrogenase(G-6-PDH) was significantly increased in the hexane and water fractions treated rats. In conclusion, these results indicated that the hexane and water fractions of LJ were effective for the antidiabetes in the STZ-induced diabetic rats.

• Journal of Veterinary Clinics
• /
• v.23 no.2
• /
• pp.202-206
• /
• 2006

A 7-year-old, spayed female, Yorkshire terrier dog with polyuria/polydipsia (PU/PD), interdigital edema, pruritus and abdominal enlargement was referred to the Veterinary Medical Teaching Hospital of the present university. Pituitary-dependent hyperadrenocortisim (PDH) was diagnosed by clinical signs, physical examination, laboratory examination (complete blood count, serum chemistry, urinalysis, ACTH stimulation test and high dose dexamethasone suppression test) and diagnostic imaging (radiography, ultrasonography and computed tomography). Clinical signs were improved after trilostane treatment, and maintenance therapy with trilostane still continued after successful induction therapy. Trilostane can be used as an alternative to mitotane therapy in hyperadrenocortisim in dogs.

• Environmental Analysis Health and Toxicology
• /
• v.18 no.4
• /
• pp.305-310
• /
• 2003

A rapid, inexpensive enzymatic method is proposed for indirect water quality testing in terms of heavy metal toxicity. The activity of glucose-6-phosphate dehydrogenase was applied for heavy metal toxicity test as an effective criterion in water quality. The toxicity of Pb (lead) and Cd (cadmium) for water flea, Moina macrocopa, were evaluated for 2-8 days with variables of mobilization ability. And the reproduction impairment of Moina macrocopa were investigated as the parameter of chronic toxicity test for Pb and Cd. As a result, the EC$_{50}$ for immobilization of Moina macrocopa were Pb and Cd were 1.6749 and 0.4683, respectively. The values of reproductive impairment to Moina macrocopa for Pb and Cd were 9.5938 and 8.3264 in EC$_{50}$ A significant alteration of G6PDH (Glucose-6-phosphate dehydrogenase) activity of Moina macrocopa was observed when Cd and Pb were treated in media. The results obtained indicate that G6PDH activity of Moina macrocopa can be used as an indicative parameter in aquatic toxicity tests for heavy metals.als.