• Title/Summary/Keyword: PDC

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Growth Characteristics of a Pyruvate Decarboxylase Mutant Strain of Zymomonas mobilis (Pyruvate decarboxylase 돌연변이 Zymomonas mobilis 균주의 생장 특성 연구)

  • Xun, Zhao;Peter L., Rogers;Kwon, Eilhann E.;Jeong, Sang Chul;Jeon, Young Jae
    • Journal of Life Science
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    • v.25 no.11
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    • pp.1290-1297
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    • 2015
  • Studies of the inactivation of a gene encoding pyruvate decarboxylase, pdc, in an ethanol-producing bacterium, Zymomonas mobilis, identified a mutant strain with 50% reduced PDC activity. To evaluate the possibility of a carbon-flux shift from an ethanol pathway toward higher value fermentation products, including pyruvate, succinate, and lactate, fermentation studies were carried out. Despite attempts to silence pdc expression in the wild-type strain ZM4 using cat-inserted pdc and pdc-deleted homologs by electroporation, the strain isolated showed partial gene activation. Fermentation experiments with the PDC mutant strain showed that the reduced expression level of PDC activity resulted in decreased rates of substrate uptake and ethanol production, together with increased pyruvate accumulation of 2.5 g l-1 , although lactate and succinate concentrations were not significantly enhanced in these modified strains. Despite numerous attempts, no strains were isolated in which complete pdc inactivation occurred. This result indicates that the ethanol fermentation pathway of this bacterium is totally dependent on the activity of the PDC enzyme. To ensure a redox balance of intracellular NAD and NADH levels, other enzymes, such as lactate dehydrogenase for lactate, and enzymes involved in the production of succinic acid, such as pyruvate dehydrogenase (PDH) and malic enzymes, may be needed for their increased end-product production.

Study on Influences and Elimination of Test Temperature on PDC Characteristic Spectroscopy of Oil-Paper Insulation System

  • Liu, Xiao;Liao, Ruijin;Lv, Yandong;Liu, Jiefeng;Gao, Jun;Hao, Jian
    • Journal of Electrical Engineering and Technology
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    • v.10 no.3
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    • pp.1107-1113
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    • 2015
  • Test temperature is an important factor affecting the measurement results of dielectric response of field power transformers. In order to better apply the polarization and depolarization current (PDC) to the condition monitoring of oil-paper insulation system in power transformers, the influences and elimination method of test temperature on PDC characteristic spectroscopy (PDC-CS) were investigated. Firstly, the experimental winding sample was measured by PDC method at different test temperatures, then the PDC-CS was obtained from the measurement results and its changing rules were discussed, which show that the PDC-CS appears a horizontal mobility with the rise of temperature. Based on the rules, the “time temperature shift technique” was introduced to eliminate the influence of test temperature. It is shown that the PDC-CS at different test temperatures can be converted to the same reference temperature coincident with each other.

Robust Trajectory Tracking Control of a Mobile Robot Combining PDC and Integral Sliding Mode Control (PDC와 적분 슬라이딩 모드 제어를 결합한 이동 로봇의 강인 궤도 추적 제어)

  • Park, Min-soo;Park, Seung-kyu;Ahn, Ho-kyun;Kwak, Gun-pyong;Yoon, Tae-sung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.7
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    • pp.1694-1704
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    • 2015
  • In this paper, a robust trajectory tracking control method of a wheeled mobile robot is newly proposed combining the PDC and the ISMC. The PDC is a relatively simple and easy control method for nonlinear system compared to the other non-linear control methods. And the ISMC can have robust and stable control characteristics against model uncertainties and disturbances from the initial time by placing the states on the sliding plane with desired nominal dynamics. Therefore, the proposed PDC+ISMC trajectory tracking control method shows robust trajectory tracking performance in spite of external disturbance. The tracking performance of the proposed method is verified through simulations. Even though the disturbance increases, the proposed method keeps the performance of the PDC method when there is no disturbance. However, the PDC trajectory tracking control method has increasing tracking error unlike the proposed method when the disturbance increases.

Removal of Styrene Using Different Types of Non-Thermal Plasma Reactors (저온플라즈마 반응기의 형태에 따른 스타이렌 분해 특성에 관한 연구)

  • Park, Jeong-Uk;Choi, Kum-Chan;Kim, Hyun-Ha;Ogata, Atsushi;Futamura, Shigeru
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.2
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    • pp.215-223
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    • 2005
  • Non-thermal plasma decomposition of gas-phase styrene was investigated in this study using three different types of plasma reactors; dielectric-barrier discharge (DBD) reactor, surface discharge (SD) reactor and plasma-driven catalyst (PDC) reactor packed with 2.0 wt% $Ag/TiO_2$ catalysts. The main parameters used for the comparative assessment of the plasma reactors include the decomposition efficiency, carbon balance, byproduct distribution, COx ($CO+CO_2$) selectivity and COx yield. The SD and the DBD reactors showed better conversion efficiency of styrene than that of the PDC reactor due to their larger capability in ozone formation. On the other hand, the PDC reactor showed better carbon balance, the yield and the selectivity of COx. The required specific input energies to achieve 100% carbon balance from the decomposition of 100 ppmv styrene using the plasma alone reactors and the PDC reactor were 420 J/L and 110 J/L, respectively. The major decomposition products in gas-phase were CO, $CO_2$ and HCOOH regardless of the types of plasma reactors. In the case of SD and DBD reactors, the $CO_2$ selectivity ranged in $39.5{\sim}60%$. The $CO_2$ selectivity in the PDC reactor was in range of $68.5{\sim}75.5%$.

Comparison of Growth Characteristics and Ginsenosides Content of 6-Year-Old Ginseng (Panax ginseng C. A. Meyer) by Drainage Class in Paddy Field (논토양에서 배수등급별 6년근 인삼의 생육특성 및 진세노사이드 함량 비교)

  • Lee, Sung-Woo;Park, Jin-Myeon;Kim, Geum-Soog;Park, Kee-Choon;Jang, In-Bok;Lee, Seung-Ho;Kang, Seung-Won;Cha, Seon-Woo
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.3
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    • pp.177-183
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    • 2012
  • To develop the practical cultivation for paddy field, we investigated the properties of paddy soil, growth characteristics and ginsenoside content of 6-year-old ginseng, Cheonpung variety between poor drainage class (PDC) and imperfect drainage class (IDC). Groundwater level in PDC showed monthly small changes of 20~30 cm, while IDC showed monthly great changes of 28~71 cm depending on rainfall. Soil moisture content in PDC and IDC was 17.2%, 22.5%, respectively. Air temperature in IDC was lower than $0.3^{\circ}C$, while soil temperature was higher than $0.8^{\circ}C$ compare to PDC, respectively. Main soil color of PDC was grayish olive, while IDC was brownish olive. PDC showed yellowish mottles only at underground of 20~40 cm, while IDC showed that at underground of 30~90 cm. IDC showed lower pH, EC, potassium, calcium and magnesium content, but higher organic matter, phosphate, and iron content than that of PDC, respectively. All of EC, organic matter, potassium, calcium, and magnesium content were decreased, but iron content was increased at the subsoil layers of PDC. All of EC, organic matter, phosphorus, and potassium content were decreased, but calcium and magnesium content were increased at the subsoil layers of IDC. Root yield in IDC was more increased by 33% than that of PDC. The moisture content and rusty ratio of ginseng root in IDC were lower than that of PDC. Ginsenoside content in IDC was higher than that of PDC because the ratio of lateral and fine root showing relatively high content of ginsenoside was higher in IDC than that of PDC.

Automated Analysis for PDC-R Technique by Multiple Filtering (다중필터링에 의한 PDC-R 기법의 자동화 해석)

  • Joh, Sung-Ho;Rahman, Norinah Abd;Hassanul, Raja
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.3C
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    • pp.141-148
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    • 2010
  • Electrical noises like self potential, burst noises and 60-Hz electrical noises are one of the causes to reduce reliability of electrical resistivity survey. Even the PDC-R (Pseudo DC resisitivity) technique, recently developed, is suffering from the problem of low reliability due to electrical noises. That is, both DC-based and AC-based resistivity technique is subject to reliability problem due to electrical noises embedded in urban geotechnical sites. In this research, a new technique to enhance reliability of the PDC-R technique by minimizing influence of electrical noises was proposed. In addition, an automated procedure was also proposed to facilitate data analysis and interpretation of PDC-R measurements. The proposed technique is composed of two steps: 1. to extract information only related with the input current by means of multiple-filter technique, and 2. to undertake a task to sort out signal information only to show stable and reliable characteristics. This automated procedure was verified by a synthetic harmonic wave including DC shift, burst random noises and 60-Hz electrical noises. Also the procedure was applied to site investigation at urban areas for proving its feasibility and accuracy.

Development of an Wireless Aeronautical Data Link Application System, PDC, for the Korean CNS/ATM (CNS/ATM 구축을 위한 항공 이동 데이터 링크 응용 시스템의 구축에 관한 연구)

  • Kim, Yong-Joong;Park, Hyo-Dal
    • Journal of Advanced Navigation Technology
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    • v.3 no.2
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    • pp.185-195
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    • 1999
  • 항공이동통신분야에서는 아날로그형 음성통신의 한계점을 극복하고, 운용자의 업무 부담을 경감할 목적으로 항공이동데이터통신망(VDL)을 이용한 데이터 통신이 항공운용통신(AOC) 분야에서 발전되었으며, 이를 항공교통서비스(ATS) 분야에 적용하기 위한 연구가 국제민간항공기구(ICAO)의 CNS/ATM 구축 권고에 의하여 미국, 유럽 등 항공 선진국에서 진행되고 있다. 본 논문은 항공교통서비스 분야에 VDL을 이용한 데이터 링크 서비스를 제공하기 위하여 디지털형의 출발전비행허가(PDC) 시스템을 개발한 내용의 결과이다. 개발된 PDC 시스템은 하나의 PC급 단일 시스템으로 항공교통관제소, VDL 통신망, 관제사 등이 원격으로 연동되어 실시간 및 동시에 PDC 정보를 처리하여야 한다. 이를 위하여 제안된 기법은 하나의 프로세서에 여러 개의 쓰레드를 갖는 멀티 쓰레드 방식이 도입되어 설계되었다. 멀티 쓰레드 방식이 사용된 이유는 시스템의 간소화, 효율적인 내부 자원의 이용, 데이터 처리에 있어서 우수한 성능을 발휘하기 때문이다. 본 논문은 김포공항 PDC 시스템의 구조 및 설계 내용을 기술하고, 실제 운용 결과에 대한 데이터를 분석하였다. 김포공항 PDC 시스템의 운용결과를 이용한 분석한 결과, 운용초기단계로 모든 항공기에 시스템이 구축되지 않아 매일 평균 25대의 항공기가 운용하여 평균 10%의 이용률을 나타내며, 최대 34대의 항공기가 이용하고 있다. 메시지 송/수신에 걸리는 시간을 분석한 결과, 항공기의 PDC 요청부터 MAS의 수신에 소요되는 시간은 최소 14초부터 최대 983초(16분 23초)까지로써, 평균 122초(2분 2초)의 시간을 나타내었다. 또한 신뢰성을 확인하기 위하여 가용도를 계산한 결과 99.7%로써 상당히 신뢰할 수 있는 수준이었다. 또한, 다중 처리를 위하여 사용된 방식인 멀티 쓰레드 방식이 적절히 동작함을 확인할 수 있었다. 앞으로, 전체 연동 시스템이 자동화로 구축될 경우, PDC의 요청부터 발송 및 확인까지 상당히 짧은 시간 내에 이루어져 관제업무 능률을 향상시킬 수 있을 것이다.

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L-trans-pyrrolidine-2,4-dicarboxylate (PDC) induces Excitotoxic and Oxidative Neuronal Death in Cultured Cortical Neurons

  • Choi, Seung-Joon;Hwang, Shin-Ae;Kim, Do-Kyung;Kim, Jong-Keun
    • International Journal of Oral Biology
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    • v.34 no.2
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    • pp.97-103
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    • 2009
  • L-trans-pyrrolidine-2,4-dicarboxylate (PDC) is a potent inhibitor of glutamate transporters. In our current study, we investigated whether the neuronal death induced by PDC involves mechanisms other than excitotoxicity in mixed mouse cortical cultures. Cortical cultures at 13-14 days in vitro were used and cell death was assessed by measuring the lactate dehydrogenase efflux into bathing media. Glutamate and PDC both induced neuronal death in a concentration-dependent manner but the neurotoxic effects of glutamate were found to be more potent than those of PDC. Treatment with 10, 100 and 200 ${\mu}$M PDC equally potentiated 50 ${\mu}$M glutamate-induced neuronal death. The neuronal death induced by 75 ${\mu}$M glutamate was almost abolished by treatment with the NMDA antagonists, MK-801 and AP-5, but was unaffected by NBQX (an AMPA antagonist), trolox (antioxidant), BDNF or ZVAD-FMK (a pan-caspase inhibitor). However, the neuronal death induced by 200 ${\mu}$M PDC was partially but significantly attenuated by single treatments with MK-801, AP-5, trolox, BDNF or ZVAD-FMK but not NBQX. Combined treatments with MK-801 plus trolox, MK-801 plus ZVAD-FMK or MK-801 plus BDNF almost abolished neuronal death, whereas combined treatments with trolox plus ZVADFMK, trolox plus BDNF or ZVAD-FMK plus BDNF did not enhance the inhibitory action of any single treatment with these drugs. These results demonstrate that the neuronal death induced by PDC involves not only in the excitotoxicity induced by the accumulation of glutamate but also the oxidative stress induced by free radical generation. This suggests that apoptotic neuronal death plays a role in PDCinduced oxidative neuronal injury.

Rock cutting behavior of worn specially-shaped PDC cutter in crystalline rock

  • Liu, Weiji;Yang, Feilong;Zhu, Xiaohua;Zhang, Yipeng;Gong, Shuchun
    • Geomechanics and Engineering
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    • v.31 no.3
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    • pp.249-263
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    • 2022
  • The specially-shaped Polycrystalline Diamond Compact (PDC) cutter is widely used in drill bit design due to its advantages of high rock cutting efficiency, strong impact resistance and long service life in hard and abrasive formation drilling. A detailed understanding of rock cutting behavior of worn specially-shaped PDC cutter is essential to improve the drilling efficiency and decrease the drilling costs. In this paper, the theoretical models of two new principles (loading performance (LP) and cutting performance (CP)) are derived for evaluating the cutting process of worn specially-shaped cutter, the theoretical models consider the factors, such as cutter geometry, aggressiveness, stress state, working life, and rock cutting efficiency. Besides, the numerical model of heterogeneous granite is developed using finite element method combined with Voronoi tessellation, the LP and CP of 12 kinds of worn specially-shaped PDC (SPDC) cutters are analyzed. The results found that the mechanical specific energy (MSE) of worn cutters first increase and then decrease with increasing the cutting depth, and the MSE increase with the increase of back rake angle except for Conical cutter and Wedge-shaped cutter. From the perspective of CP, the worn PDC cutters are more suitable for the smaller cutting depths, and the back rake angle has little effect on the CP of the specially-shaped worn PDC cutters. Conical cutter, Saddle-shaped cutter and Ellipse-shaped cutter have the highest CP value, while Rhombus-shaped cutter, Convex cutter and Wedge-shaped cutter have the lowest value in selecting cutters. This research leads to an enhanced understanding of rock-breaking mechanisms of worn SPDC cutters, and provides the basis to select of specially-shaped PDC cutters for the specific target formation.