• Title/Summary/Keyword: PCV2

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Hemoglobin Concentration and Hematocrit Value of Black Bengal Goats Infected with Fasciola gigantica

  • Howlader, M.M.R.;Huq, M.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.1
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    • pp.118-121
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    • 1997
  • A total of 72 Black Bengal goats of 2.5 to 3.5 and 4.0 to 6.0 years old were used in this study. Equal number of animals were included in Fasciola gigantica infected and non-infected control groups. For each age and treatment groups 18 blood samples were collected in glass vials contained EDTA anticoagulant in summer and winter seasons before the animals were slaughtered at abottoir. Packed cell volume (PCV) was determined using microhematocrit and hemoglobin (Hb) concentration by cyanmethemoglobin methods. The PCV of F. gigantica infected animals were significantly lower than the non-infected animals. The average PCV values obtained were 26.60 and 32.20% for F. gigantica infected and non-infected animals, respectively. The Hb values of infected animals were significantly lower than the non-infected animals. The average Hb values obtained were 9.17 and 10.51 gm% for F. gigantica infected and non-infected goats, respectively. There was no significant effect of age and season on the values of PCV and Hb of infected and non-infected animals.

Classification of Porcine Wasting Diseases Using Sound Analysis

  • Gutierrez, W.M.;Kim, S.;Kim, D.H.;Yeon, S.C.;Chang, H.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.8
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    • pp.1096-1104
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    • 2010
  • This bio-acoustic study was aimed at classifying the different porcine wasting diseases through sound analysis with emphasis given to differences in the acoustic footprints of coughs in porcine circo virus type 2 (PCV2), porcine reproductive and respiratory syndrome (PRRS) virus and Mycoplasma hyopneumoniae (MH) - infected pigs from a normal cough. A total of 36 pigs (Yorkshire${\times}$Landrace${\times}$Duroc) with average weight ranging between 25-30 kg were studied, and blood samples of the suspected infected pigs were collected and subjected to serological analysis to determine PCV2, PRRS and MH. Sounds emitted by coughing pigs were recorded individually for 30 minutes depending on cough attacks by a digital camcorder placed within a meter distance from the animal. Recorded signals were digitalized in a PC using the Cool Edit Program, classified through labeling method, and analyzed by one-way analysis of variance and discriminant analysis. Input features after classification showed that normal cough had the highest pitch level compared to other infectious diseases (p<0.002) but not statistically different from PRRS and MH. PCV2 differed statistically (p<0.002) from the normal cough and PRRS but not from MH. MH had the highest intensity and all coughs differed statistically from each other (p<0.0001). PCV2 was statistically different from others (p<0.0001) in formants 1, 2, 3 and 4. There was no statistical difference in duration between different porcine diseases and the normal cough (p>0.6863). Mechanisms of cough sound creation in the airway could be used to explain these observed acoustic differences and these findings indicated that the existence of acoustically different cough patterns depend on causes or the animals' respiratory system conditions. Conclusively, differences in the status of lungs results in different cough sounds. Finally, this study could be useful in supporting an early detection method based on the on-line cough counter algorithm for the initial diagnosis of sick animals in breeding farms.

Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets - I. Serological result, FA test and RT-PCR - (포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 - I. 혈청학적 결과, 형광항체검사 및 RT-PCR 검사 -)

  • Jin, Wen;Kim, Yeong-Hun;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.177-187
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    • 2009
  • The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. The results obtained were as follows: Antibodies against PCV2 and PEDV were detected at 24, 36, 48, 60 and 72h postinfection. And the antibody titers of alone infections with PEDV were gradually reduced and increased from 60 hpi to 72 hpi. Whereas, the antibody titers of dual infections with PCV2 and PEDV were gradually reduced all the time. PEDV antigens were detected at 24, 36, 48, 60 and 72 hpi, being seen almost exclusively in feces and small intestines from PEDV-infected piglets and PCV2-coinfected piglets. The detection rate of PEDV in feces and jejunum tissues by RT-PCR were 94.9% and 91.1% in dual infections and 87.1% and 83.6% in alone infections with PEDV, respectively. In dual infected piglets, significantly more PEDV antigens were detected in the feces and small intestines tissues at 24 hpi (P<0.05) than in the same feces and tissues of the alone infected piglets. Thereafter, at 72 hpi significantly more PEDV antigens (P<0.05) was detected in the jejunal tissues of the dual infected piglets with than of alone PEDV-infected piglets. The detection rate of PEDV antigen in the duodenum, jejunum and ileum by IFA were 91.3%, 91.3% and 83.3% in dual infected piglets and 75.0%, 83% and 75% in alone infected piglets, respectively. Intense and specific fluorescence signals were more often seen within jejunal villous enterocytes in dual infected piglets than alone infected piglets.

EFFECTS OF XYLAZINE (ALPHA 2-ADRENERGIC AGONIST) ON THE STRESS RESPONSE TO IMMOBILIZATION AND HEAT IN RATS

  • Fayed, A.H.;Zakaria, A.D.;Hedaya, S.A.;El-Ashmawy, I.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.3
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    • pp.397-400
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    • 1994
  • The effect of xylazine administration on plasma cortisol, prolactin, glucose and packed cell volume (PCV) responses to immobilization and heat stress was investigated. Immobilization of rats for 2 hours by ligation of the fore and hind legs strongly caused approximately two-fold increase in plasma cortisol and prolactin levels. Plasma glucose and PCV were not significantly changed. Pretreatment of immobilized rats with xylazine (20 mg/kg body weight i.m.) resulted in approximately 20% reduction in both plasma cortisol and prolactin concentrations. A marked hyperglycemia and increase in the PCV value was observed. On the other hand, rats exposed to acute heat stress ($40^{\circ}C$, and 60% relative humidity) for 2 hours, also developed two fold increase in both plasma cortisol and prolactin concentrations and the pretreatment with xylazine caused a 20% reduction in the levels of both hormones. Plasma glucose level was not significantly changed in heat stressed rats but it was markedly increased after pretreatment with xylazine. PCV was significantly incrcased under heat stress and pretreatment with xylazine induced a pronounced elevation in this value. It was suggested that stimulation of cortisol and prolactin secretion in response to immobilization or heat stress can be partially reduced by an alpha 2-adrenergic agonist.

Investigation of post - weaning atrophic pig diseases in swine breeding complex in Jeonbuk - Iksan (전북 익산 양돈단지 이유 후 위축자돈 질병 조사)

  • Chu, Keum-Suk;Jo, Young-Suk
    • Korean Journal of Veterinary Service
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    • v.30 no.1
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    • pp.85-93
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    • 2007
  • The purpose of this study was to investigate the infection situation of several diseases (post-weaning atrophic pigs) such as porcine reproductive and respiratory syndrome (PRRS) in swine breeding complex in Jeonbuk-Iksan. From February to October in 2006, a total of 28 swine samples (6-10 week old) were collected from 6 farms and examined by polymerase chain reaction(PCR) and clinical signs. In the rate of single infection, pneumonia was top (32.1%), followed by salmonellosis (14.2%)and Glasser's disease (10.7%) and double infection pneumonia/Glasser's disease (17.8%) was detected. PCR was detected of PCV 2 from 28 (100.0%) and PPV 6 (21.4%), PRRS PORF6 10 (35.7%) and POR7 11 (39.2%), but HC and AD was not detected. The results suggest that PCV 2 is complex infection PRRS, PPV and bacterial disease.

An Analysis of Investigation Movies of S/C Vent Area in the Unit 2 Reactor Building Basement floor of Fukushima Daiichi Nuclear Power Plant (후쿠시마 제 1 원자력발전소 2 호기 원자로 건물 지하의 S/C Vent 관 조사영상 분석)

  • Cho, Jai-Wan;Jeong, Kyung-Min
    • Proceedings of the Korea Information Processing Society Conference
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    • 2013.05a
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    • pp.311-314
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    • 2013
  • 본 논문에서는 일본의 (주) 동경전력이 공개한 후쿠시마 제 1 원자력발전소 2 호기 원자로건물 지하에 위치한 S/C (Suppression Chamber) 주변의 Vent 관 조사 동영상들을 분석하였다. S/C 는 donut (환형) 모양의 구조물로 PCV (격납용기) 와 연결된 8 개의 Vent 관을 통해 원자로의 압력을 억제한다. 후쿠시마 사고 원자로의 용융 핵연료를 인출하기 위해서는 원자로 압력용기 및 PCV에 물을 채워서 방사선 선량율을 떨어뜨려야 한다. 물을 채운 후에 누설이 되면 안되기 때문에, PCV 와 S/C 사이에 연결된 Vent 관에 대한 방사능 오염수의 누설지점을 찾는 것이 중요하다. 이를 위한 사전공정으로 (주) 도시바의 4 족보행 로봇 탑재 카메라를 이용하여 8 개 Vent 관 모두를 육안 검사하였다. (주) 동경전력이 공개한 영상을 분석한 결과 고선량 감마선에 의한 Speckle 들이 관측되었다. 본 논문에서는 이러한 Speckle 분포의 특성을 분석하여 S/C 와 PCV 를 연결하는 8개 Vent 관중 방사능 오염물질이 많은 곳을 특정하고자 하였다.

GNSS Antenna PCO/PCV and Position Changes due to the Switch IGS08/igs08.atx to IGS14/igs14.atx

  • Choi, Byung-Kyu;Sohn, Dong-Hyo;Yoon, Ha-Su;Lee, Sang Jeong
    • Journal of Positioning, Navigation, and Timing
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    • v.11 no.2
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    • pp.83-89
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    • 2022
  • For precise GNSS applications, the antenna phase center correction (PCC) is absolutely required. The PCC magnitude can reach the centimeter level with the antenna structure. In the present study, we first investigate the phase center offset (PCO) and phase center variation (PCO) of three different antenna models in two different reference frames, IGS08/igs08.atx and IGS14/igs14.atx. Clear L1 and L2 PCO differences were found between IGS08 and IGS14. In addition, the PCV showed characteristics that is dependent upon the signal direction (azimuth and elevation angle). The remarkable thing is that the changes of a Dorne Margolin choke-ring antenna model (AOAD/MT DOME) was very small in two reference frames. In order to analyze changes in positions according to different reference systems, GNSS data obtained from DAEJ, SUWN, and TSKB stations were processed by the precise point positioning (PPP) method. We suggest that an antenna PCO/PCV can affect the precise GNSS positioning on the order of several millimeters in two different reference frames.

High Ejaculation Frequency Enhances Semen Production in Taiwan Country Chickens

  • Fan, Y.K.;Ju, J.C.;Lee, S.L.;Chen, C.F.;Peh, H.C.;Hsu, J.C.;Lee, Y.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.924-929
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    • 2004
  • The objective of this study were to investigate the effect of ejaculation frequency on semen characteristics and to establish a method for quick assessment of sperm concentration in TCC using packed cell volume (PCV) as the parameter (Trial 1). Eighty senior roosters, averaging 61 wk-old, were used and the sperm concentrations were determined using a hemacytometer. The PCV value was measured in a capillary (0.75 mm in inner diameter) by centrifugation. A simple linear regression analysis suggested that the sperm concentrations were significantly correlated with PCV values (r=0.62, p<0.001). Trial 2 was conducted to determine the optimal ejaculation frequency of TCC roosters in a weekly semen collection program. The male birds were subjected to 1, 2, 3 or 6 ejaculations per week for four consecutive weeks and semen characteristics including ejaculation volume (EV, mL), sperm motility (%), PCV (%), sperm concentration (ESC, $\times$10$^{9}$/mL), weekly sperm production (WSP, $\times$10$^{9}$/wk) and average motile sperm numbers (AMSN, $\times$10$^{9}$/ejac) were determined. Average EV was greater in the group with 3 ejac/wk than with only 1 ejac/wk in weeks 1 and 3 of the collection period. WSP increased with ejaculation frequency during the first 3 weeks of collection (p<0.05). Sperm motility was better in the birds with 6 ejac/wk than in single ejaculation group for the first 2 wk and no significant differences were found for the last 2 wk of study. In contrast, the PCV value showed a trend of reduction for the first 2 wks in the 6 ejac/wk group. Surprisingly, no significant differences were detected in the AMSN among treatment groups. The weekly motile sperm production (WMSP) increased with ejaculation frequency. Based on our observation, PCV values could be used for a quick estimation of sperm concentration and an intensive semen collection program enhanced weekly sperm production in TCC roosters.

The Evolving Epidemiology of Serotype Distribution and Antimicrobial Resistance of Streptococcus pneumoniae Strains Isolated from Adults in Crete, Greece, 2009-2016

  • Maraki, Sofia;Mavromanolaki, Viktoria Eirini;Stafylaki, Dimitra;Hamilos, George;Samonis, George
    • Infection and chemotherapy
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    • v.50 no.4
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    • pp.328-339
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    • 2018
  • Background: Pneumococcal disease is a major cause of morbidity and mortality worldwide, especially in patients with comorbidities and advanced age. This study evaluated trends in epidemiology of adult pneumococcal disease in Crete, Greece, by identifying serotype distribution and antimicrobial resistance of consecutive Streptococcus pneumoniae strains isolated from adults during an 8-year time period (2009-2016) and the indirect effect of the infant pneumococcal higher-valent conjugate vaccines 10-valent pneumococcal conjugate vaccine (PCV10) and 13-valent pneumococcal conjugate vaccine (PCV13). Materials and Methods: Antimicrobial susceptibility was performed by E-test and serotyping by Quellung reaction. Multidrug resistance (MDR) was defined as non-susceptibility to penicillin (PNSP) combined with resistance to ${\geq}2$ non-${\beta}$-lactam antimicrobials. Results: A total of 135 S. pneumoniae strains were isolated from adults during the study period. Twenty-one serotypes were identified with 17F, 15A, 3, 19A, and 11A, being the most common. The coverage rates of PCV10, and PCV13 were 17.8% and 37.8%, respectively. PCV13 serotypes decreased significantly from 68.4% in 2009 to 8.3% in 2016 (P = 0.002). The most important emerging non-PCV13 serotypes were 17F, 15A, and 11A, with 15A being strongly associated with antimicrobial resistance and MDR. Among all study isolates, penicillin-resistant and MDR strains represented 7.4% and 14.1%, respectively. Predominant PNSP serotypes were 19A (21.7%), 11A (17.4%), and 15A (17.4%). Erythromycin, clindamycin, tetracycline, trimethoprim-sulfamethoxazole, and levofloxacin resistant rates were 30.4%, 15.6%, 16.3%, 16.3%, and 1.5%, respectively. Conclusion: Although pneumococcal disease continues to be a health burden in adults in Crete, our study reveals a herd protection effect of the infant pneumococcal higher-valent conjugate vaccination. Surveillance of changes in serotype distribution and antimicrobial resistance among pneumococcal isolates are necessary to guide optimal prevention and treatment strategies.

Effect of Dextrose, NaCl and Temperature on the RBC Sedimentation Rates in Goat (산양적혈구(山羊赤血球) 침강속도(沈降速度)에 미치는 온도(溫度), NaCl 및 Dextrose에 대하여)

  • Yu, Chang Jun
    • Current Research on Agriculture and Life Sciences
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    • v.2
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    • pp.109-114
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    • 1984
  • Eight Korean native black goats were used, $10m{\ell}$ of blood was collected from the Jugular vein into heparinized tubes a week interval. The heparinized blood was centrifuged for separation to blood plasma and corpuscles. The hematocrit, per centage of blood that is red blood cells, was reschuffuled of 10 %, 20 %, 30 %, 40 % and 50 % using blood plasma, 0.9 % NaCl solution and 5.4 % dextrose solution. The sedimentation rates of red blood cell obtained at $7^{\circ}C{\pm}1^{\circ}C$ and $27^{\circ}C{\pm}1^{\circ}C$ are summarized as follows. 1) The sedimentation rates of red blood cell were more increased by lower PCV%, i.e. there was a reverse relationship between the sedimentation rates and PCV% at any condition of these experiments. 2) The RBC were sedimented the most quickly in the NaCl solution and slower in the plasma compare with the dextrose solution at the same PCV%. 3) There was no temperature effect on the sedimentation rates between the two groups of $7^{\circ}C{\pm}1^{\circ}C$ and $27^{\circ}C{\pm}1^{\circ}C$(at PCV 20% and 10%), even though the temperature difference is $20^{\circ}C$.

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