Browse > Article

Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets - I. Serological result, FA test and RT-PCR -  

Jin, Wen (School of Veterinary Medicine, Kangwon National University)
Kim, Yeong-Hun (School of Veterinary Medicine, Kangwon National University)
Han, Jeong-Hee (School of Veterinary Medicine, Kangwon National University)
Publication Information
Korean Journal of Veterinary Service / v.32, no.3, 2009 , pp. 177-187 More about this Journal
Abstract
The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. The results obtained were as follows: Antibodies against PCV2 and PEDV were detected at 24, 36, 48, 60 and 72h postinfection. And the antibody titers of alone infections with PEDV were gradually reduced and increased from 60 hpi to 72 hpi. Whereas, the antibody titers of dual infections with PCV2 and PEDV were gradually reduced all the time. PEDV antigens were detected at 24, 36, 48, 60 and 72 hpi, being seen almost exclusively in feces and small intestines from PEDV-infected piglets and PCV2-coinfected piglets. The detection rate of PEDV in feces and jejunum tissues by RT-PCR were 94.9% and 91.1% in dual infections and 87.1% and 83.6% in alone infections with PEDV, respectively. In dual infected piglets, significantly more PEDV antigens were detected in the feces and small intestines tissues at 24 hpi (P<0.05) than in the same feces and tissues of the alone infected piglets. Thereafter, at 72 hpi significantly more PEDV antigens (P<0.05) was detected in the jejunal tissues of the dual infected piglets with than of alone PEDV-infected piglets. The detection rate of PEDV antigen in the duodenum, jejunum and ileum by IFA were 91.3%, 91.3% and 83.3% in dual infected piglets and 75.0%, 83% and 75% in alone infected piglets, respectively. Intense and specific fluorescence signals were more often seen within jejunal villous enterocytes in dual infected piglets than alone infected piglets.
Keywords
PEDV; PCV2; Dual infected; Piglets;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chasey D, Cartwright SF. 1978. Virus-like particles associated with porcine epidemic diarrhea. Res Vet Sci 25: 255-256   PUBMED   ScienceOn
2 Cheung AK. 2003. Transcriptional analysis of pocine circovirus. J Virol 305: 168-180   DOI   PUBMED   ScienceOn
3 Kim O, Choi B, Kim B, Chae C. 2000. Detection an differentiation of porcine epidemic diarrhea virus and transmissble gastroenteritis virus in clinical samples by multiplex RT-PCR. Vet Rec 146: 637-640   DOI   PUBMED   ScienceOn
4 McNulty M, Dale J, Lukert P. 2000 Circovirida. In: van Regenmortl MHV, Fauquet CM, Bishop DHL. Seventh Report of the International Committee on Taxonomy of Viruses. San Diego : Academic Press: 299-303
5 Mankertz A, Persson F, Mankertz J, Blaess G, Buhk HJ. 1997 Mapping and characterization of the origin of the replication of porcine circovirus. J Virol 71: 2562-2566   PUBMED
6 Pensaert MB, DeBouck P. 1978. A new coronavirus-like particle associated with diarrhea in swine. Arch Virol 58: 243-247   DOI   ScienceOn
7 West KH, Bystrom JM. 1999. Mycocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2. J Vet Diagn Invest 11: 530-532   DOI   PUBMED   ScienceOn
8 Fenaux M, Halbur PG, Gill M, Toth TE, Meng XJ. 2000. Genetic characterization of type 2 pocine circovirus (PCV-2) from pigs with post-weaning multisystemic wasting syndrome in dìfferent geographic regions of North America and development of a differential PCRrestriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38: 2494-2503   PUBMED
9 Allan GM, Ellis JA. 2000. Porcine circoviruses: a review. J Vet Diagn Invest 12(1): 3-14   DOI   PUBMED   ScienceOn
10 Hofmann M, Wyler R. 1988. Propagatìon of the virus of porcine epidemic diarrhea in cell culture. J Clin Microbiol 26: 2235-2239
11 律田知幸. 1999. 隊流行性下痢. 隊病學, 第7版, 近代出版, 東京: 260-266
12 Allan G, Krakowka S. 2002. PCV2 : Ticking time bomb. Pig Progress 18: 14-15
13 Callebaut P, DeBouck P, Pensaert M. 1982. Enzyme-linked immunosorbent assay for the detection of the corona virus-like agent and its antibodies in pigs with porcine epidemic diarrhea. Vet Microbiol 7: 295-306   DOI   ScienceOn
14 Pensaert MB. 1999. Porcine epidemic diarrhea. Disease of swine 8th ed. Iowa State Univ Press, Ames, Iowa, USA : 179-185
15 Carvajal A, Lanza I, Diεgo R, Rubio P, Carrnenes P. 1995. Evaluation of a blocking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhea virus and its antibodies. J Vet Diagn Invest 7: 60-64   DOI   PUBMED   ScienceOn
16 Ellis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Has-sard L, Martin K, McNeilly F. 2000. Coinfection by porcine acquired postweaning muItisystemic wasting syndrome. J Vet Diagn Invest 12: 21-27   DOI   PUBMED   ScienceOn
17 Rosell C, Segales J, Ramos-Vara JA, Folch JM, RodriguezArrioja GM, Duran CO, Balasch M, Plana-Duran J, Domingo M. 2000. Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Vet Rec 146: 40-43   DOI   PUBMED   ScienceOn
18 Hirai T, Nunoya T. 2006. Infectivity of porcine Clrcovlrus and circovirus 2 in primary porcine hepatocyte and kidney cell cultures. J Vet Med Sci 68(2): 179-182   DOI   ScienceOn
19 Jung K, Ha Y, Ha SK, Kim J, Choi C, Park HK, Kim SH, Chae C. 2006. IdentificatÌon of porcine cìrcovirus type 2 in retrospective cases of pigs naturally infected with porcine epidemic diarrhoea virus. Vet J 171(1): 166-168   DOI   ScienceOn
20 Allan GM, McNeilly F, Meehan BM. 1999. Isolation and characterization of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbiol 66: 115-123   PUBMED
21 Pensaert MB, DeBouck P. 1978. A new coronavirus-like particle associated with diarrhea in swine. Arch Virol 58: 243-247   DOI   ScienceOn
22 Allan GM, McNeilly F, Kennedy S, Kennedy S, Clarke EG, Ellis JA, Haines DM, Meehan BM, Adair BM. 1998. Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Invest 10: 3-10   DOI   PUBMED   ScienceOn
23 Hamel AL, Lin LL, Nayar GP, et al. 1998. Nucleotide sequence of porcine circovirus associated wìth postweaning multisystemic wasting syndrome in pigs. J Virol 72(6); 5262-5267   PUBMED
24 Hofmann M, Wyler R. 1989. Quantitation, biological and physicochemical properties of ceII culture-adapted porcine epidemic diarrhea coronavirus (PEDV). Vet Microbiol 20: 131-142   DOI   ScienceOn
25 Krakowka S, Ellis JA, Meehan B, Kennedy S, McNeilIy F, Allan G. 2000. Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet Pathol 37: 254-263   DOI   ScienceOn
26 Timoney JF, Gìllespie JH, Scott FW, Barlough JE. 1988. Hagan and Bruner's microbiology and infectiouts diseases of domestic animals. 8th ed. Comstock Publìshing Associates, Ithaca, USA: 897-898
27 Harding C. 2004. The clinical expression and emergence of porcine circovirus 2. Vet Microbiol 98: Vet Microbiol
28 DeBouck P, Pensaert M. 1980. Experimental infection of pigs with a new porcine enteric coronavirus, CV777. Am J Vet Res 41: 219-223   PUBMED
29 Prager D, Witte KH. 1981. Die serologische Diagnose der Epizootischen virusdiarrhoe (EVD) des Schweines mit Hilfe der indirekten Immunofluores-zeztechnik (llFT). II. Antikorper antwort nach εxperimenteller Infection. Tierarztl Umschau 36: 477-480
30 DeBouck P, Pensaert M, Coussement W. 1981. The pathogenesis of an enteric infection in pigs experimentally induced by the coronavirus-like agent CV777. Vet Microbiol 6: 157-165   DOI   ScienceOn
31 류영수, 박최규, 장정호. 1997. 가축질병진단. 초판, 이공월드, 서울: 87-90