• Title/Summary/Keyword: PAM4

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Feeding specificity and photosynthetic activity of Korean sacoglossan mollusks

  • Klochkova, Tatyana A.;Han, Jong-Won;Kim, Ju-Hyoung;Kim, Kwang-Young;Kim, Gwang-Hoon
    • ALGAE
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    • v.25 no.4
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    • pp.217-227
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    • 2010
  • During feeding on algal cytoplasm, some sacoglossans are known to keep the chloroplasts photosynthetically active for days to months in their digestive cells. Korean sacoglossan mollusks containing functional chloroplasts were screened using an in vivo chlorophyll fluorescence measuring system (pulse amplitude modulation, PAM). We collected six sacoglossans feeding on siphonous and siphonocladous green algae (Elysia atroviridis, E. nigrocapitata, E. ornata, Ercolania boodleae, Placida dendritica, Stiliger sp.) and one feeding on ceramiaceaen algae (Stiliger berghi) and performed feeding experiments using 37 algal species. Three species of Elysia showed strong photosynthetic activity for months. However, P. dendritica maintained functional chloroplasts only for several hours after feeding. E. boodleae, S. berghi, and Stiliger sp. showed no photosynthetic activity in any circumstances. Among all species, E. nigrocapitata was capable to tolerate the longest period of starvation for over 4 months. Four 'solar powered' sacoglossans bonded avidly to their specific algal food. Each species attached to and consumed only one algal species when several algae were given together. While they occasionally consumed other algae after prolonged starvation, they always reverted to their specific algae when available.

Performance Improvement of 3D HDTV Transmission with Block LDPC Codes (블록 LDPC 부호를 사용한 3D HDTV 전송을 위한 성능개선 방안에 관한 연구)

  • Kim, Minki;Kim, Dongho
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2012.07a
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    • pp.134-137
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    • 2012
  • 최근 ATSC방식의 디지털 방송에서 HD급의 3D 방송 서비스에 대한 관심이 증가 되고 있는 추세이다. HD급의 3D 방송 서비스(이하 3D HDTV)를 제공하기 위해 [2]에서는 ATSC 전송 시스템을 확장하고 BCH 부호와 IRA 타입의 LDPC를 사용하는 수정된 ATSC 전송 시스템을 제안하였다. 본 논문에서는 BCH와 IRA 타입의 LDPC 부호 대신 Reed Solomon 부호와 Block LDPC 부호를 사용하고 16QAM 변조를 사용하여 전송 용량을 증대할 수 있다는 사실을 확인하였다. AWGN 채널에서 모의 실험한 결과 BCH부호와 LDPC부호 및 4PAM을 적용한 수정된 ATSC 전송시스템의 TOV는 약 7dB로 19.33Mbps로 전송되지만[2] 본 논문에서 제안한 RS(207, 187)과 부호율 3/4인 Block LDPC 및 16QAM의 TOV는 약 5.4dB로 29Mbps로 전송되어 기존시스템에 비해 약1.5배의 전송 용량 증대가 가능한 것으로 확인되었다. 이로서 6MHz의 한정된 대역에서 HD급의 3D 방송서비스 실현이 한층 더 가까워지게 될 것으로 예상된다.

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Antidotal and Neuroprotective Efficacies of a Prophylactic Patch against Sarin and Soman Poisonings in Guinea Pigs (신경작용제 사린 및 소만 중독에 대한 기니픽에서의 예방패치의 해독 및 뇌보호 효능)

  • Song, Youngjo
    • Journal of the Korea Institute of Military Science and Technology
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    • v.24 no.1
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    • pp.144-150
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    • 2021
  • This study was designed to evaluate the prophylactic efficacy of a combinational patch system containing physostigmine and procyclidine against sarin and soman using guinea pig. The median lethal dose values of two nerve agents were calculated by a probit analysis of deaths occurring within 24 h. In this study, the values of median lethal dose of sarin and soman were determined to be 33.0 and 26.7 ㎍/kg in guinea-pigs, respectively. The guinea pigs treated with a prophylactic patch(4×5 ㎠) for 24 h were 100 % protected against a challenge of 1.5 LD50. The combinational KMARK-1(atropine and 2-PAM) and prophylactic patch were more effective than a single KMARK-1, a combination of pyridostigmine and KMARK-1 significantly. Epileptiform seizures in the guinea pigs treated with the combinational antidotes led to neuropathological changes, in comparison with intact feature of brain of the animal treated with the patch.

Characterization and Gene Co-expression Network Analysis of a Salt Tolerance-related Gene, BrSSR, in Brassica rapa (배추에서 염 저항성 관련 유전자, BrSSR의 기능 검정 및 발현 네트워크 분석)

  • Yu, Jae-Gyeong;Lee, Gi-Ho;Park, Ji-Hyun;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.845-852
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    • 2014
  • Among various abiotic stress factors, soil salinity decreases the photosynthetic rate, growth, and yield of plants. Recently, many genes have been reported to enhance salt tolerance. The objective of this study was to characterize the Brassica rapa Salt Stress Resistance (BrSSR) gene, of which the function was unclear, although the full-length sequence was known. To characterize the role of BrSSR, a B. rapa Chinese cabbage inbred line ('CT001') was transformed with pSL94 vector containing the full length BrSSR cDNA. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of BrSSR in the transgenic line was 2.59-fold higher than that in the wild type. Analysis of phenotypic characteristics showed that plants overexpressing BrSSR were resistant to salinity stress and showed normal growth. Microarray analysis of BrSSR over-expressing plants confirmed that BrSSR was strongly associated with ERD15 (AT2G41430), a gene encoding a protein containing a PAM2 motif (AT4G14270), and GABA-T (AT3G22200), all of which have been associated with salt tolerance, in the co-expression network of genes related to salt stress. The results of this study indicate that BrSSR plays an important role in plant growth and tolerance to salinity.

Research for the Analytical Method of Various Pesticides in Raw Milk by Gas Chromatography-Mass Spectrometry (GC/MSD를 사용한 원유 내 잔류농약의 분석법 연구)

  • Oh, N.S.;Shin, Y.K.;Baick, S.C.
    • Food Science of Animal Resources
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    • v.29 no.4
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    • pp.482-486
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    • 2009
  • The aim of this study was to optimize a simple, fast, and economical analysis procedure for the determination of 16 different pesticides in raw milk via GC/MSD. Analyses were performed via gas chromatography with electron impact mass spectrometric detection in the selected ion monitoring mode (GC/MSD-SIM) using Pentachloronitrobenzene as the internal standard. The modified sample preparation methodology was based on the Pesticide Analytical Manual (PAM) of the FDA concerning fat extraction, ACN-ether partitioning, and clean-up of the Sep-Pak florisil cartridge. The modified methodology for the determination of the 16 pesticides was validated. The range of LOQs of the 16 pesticides was likely three times lower than their Maximum Residence Levels (MRLs). The recoveries of most of the pesticides were acceptable at the fortification levels of 0.5 and 1.0 ${\mu}g/mL$ and their RSD (%) level was less than 20%. None of the 16 pesticides were detected in the selected raw milk samples.

Influencing Factors of Continuous Use Intention of Mobile IPTV Service (모바일 IPTV 서비스의 지속적인 이용의도에 대한 영향요인)

  • Kim, Hyoseok;Kim, Sanghoon
    • Journal of Information Technology Services
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    • v.16 no.4
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    • pp.99-120
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    • 2017
  • As smartphones and tablets are drastically spreading, mobile IPTV service becomes very important emerging issue in addition to existing DMB broadcasting, and the consumption of personalized multimedia services is increasing in the mobile Internet environment. While the previous research focuses on influencing factors of the initial acceptance intention of the mobile IPTV Service, this study intends to find the factors that affect continuous use intention of the mobile IPTV service which has become one of core services in the mobile environment. The research model and the hypotheses of this study could be derived on the basis of four referent theories (that is, mobile service quality model (MOBISQUAL), post acceptance model (PAM), expectation confirmation theory (ECT) and extended technology acceptance model (ETAM)). In order to empirically test the hypotheses, the field survey was conducted for 278 respondents. Twelve ones of fifteen hypotheses were found to be statistically significantly adopted from the results of structural equation model analyses using Smart PLS 3.0. The theoretical contribution of this study is to empirically investigate the factors that affect the continuous use intention of mobile IPTV service. And the practical contribution is to provide the implementation guidelines of managing the service quality to enhance the competitive power of mobile IPTV business.

Similarity Analysis of Exports Value Added by Country and Implication for Korea's Global Value Added Chains

  • Cho, Jung-Hwan
    • Journal of Korea Trade
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    • v.23 no.4
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    • pp.103-114
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    • 2019
  • Purpose - This paper investigates the structure of exports across countries in terms of value added. Exports value added is examined under two categories, domestic and overseas. Using a statistical classification method by distance based on these two value added categories, this paper estimates the similarity of exports value added across countries including Korea. Design/methodology - The model of study is to employ a generalized distance function and then derive the Manhattan and Euclidean distances. The paper also performs cluster analysis using the Partitioning Around Medoids (PAM) and hierarchical methods to classify the 44 sample countries considered in this study. Findings - Our main findings are as follows. The 44 countries can be classified under 5 groups by their domestic and overseas value added in exports. Korea has a sandwich global value chains (GVCs) position between Japan, China, and Taiwan in the East Asian region. Originality/value - Existing papers point out the double counting problem of trade statistics as the intermediate goods trade across borders increases. This paper addresses the double counting problem by using the World Input-Output Table. The paper shows the need to explore the similarity of value added in exports structure across countries and investigate the GVCs position and role of each country.

Electroabsorption modulator-integrated distributed Bragg reflector laser diode for C-band WDM-based networks

  • Oh-Kee Kwon;Chul-Wook Lee;Ki-Soo Kim
    • ETRI Journal
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    • v.45 no.1
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    • pp.163-170
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    • 2023
  • We report an electroabsorption modulator (EAM)-integrated distributed Bragg reflector laser diode (DBR-LD) capable of supporting a high data rate and a wide wavelength tuning. The DBR-LD contains two tuning elements, plasma and heater tunings, both of which are implemented in the DBR section, which have blue-shift and red-shift in the Bragg wavelength through a current injection, respectively. The light created from the DBR-LD is intensity-modulated through the EAM voltage, which is integrated monolithically with the DBRLD using a butt-joint coupling method. The fabricated chip shows a threshold current of approximately 8 mA, tuning range of greater than 30 nm, and static extinction ratio of higher than 20 dB while maintaining a side mode suppression ratio of greater than 40 dB under a window of 1550 nm. To evaluate its modulation properties, the chip was bonded onto a mount including a radiofrequency line and a load resistor showing clear eye openings at data rates of 25 Gb/s nonreturn-to-zero and 50 Gb/s pulse amplitude modulation 4-level, respectively.

An Assessment of Primary Productivity Determined by Stable Isotopes and Diving-PAM in the Pyropia Sea Farms of the Manho (Jindo-Haenam) Region on the Southwestern Coast of the Korean Peninsula (안정동위원소 및 Diving-PAM을 이용한 남서해안 만호해역 (진도-해남) 김 양식장에서의 일차 생산력)

  • Kim, Jeong Bae;Lee, Won-Chan;Kim, Hyung Chul;Hong, Sokjin
    • Korean Journal of Environmental Biology
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    • v.34 no.1
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    • pp.18-29
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    • 2016
  • The effects of water temperature, salinity, water column nutrient contents, and phytoplankton primary productivity on pigment composition and concentration, as well as primary productivity of Pyropia yezoensis Ueda purple lavers were studied at the primary cultivation areas in the Manho (Jindo-Haenam) region on the southwestern coast of Korea in March 2014. The water temperature was $9.1{\sim}9.6^{\circ}C$, salinity was 32.5~33.1, and transparency was 0.7~1.5 m. The shallow euphotic depth resulted from the high turbidity. Water column dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), and silicate concentrations were $3.59{\sim}5.73{\mu}M$, $0.16{\sim}0.41{\mu}M$, and $12.41{\sim}13.94{\mu}M$, respectively. Chlorophyll a (Chl a) concentration was $0.51{\sim}1.25{\mu}g\;L^{-1}$. Nanoplankton ($0.7{\sim}20{\mu}m$ size class) accounted for 58% of the total Chl a concentration. Fucoxanthin was the dominant photosynthetic pigment at all sites. Microplankton ($20{\sim}200{\mu}m$ size class) accounted for 64% of the total fucoxanthin concentration. The primary productivity of phytoplankton was $57.72{\pm}4.67(51.05{\sim}66.71)mg\;C\;m^{-2}d^{-1}$. The nanoplankton ($0.7{\sim}20{\mu}m$ size class) accounted for 77% of the total phytoplankton primary productivity. The calculated phytoplankton primary productivity was $11,337kg\;C\;d^{-1}$. The primary productivity of Pyropia blades was $1,926{\pm}192(1,102{\sim}2,597)mg\;C\:m^{-2}d^{-1}$, i.e., calculated as $39,295kg\;C\;d^{-1}$. The total primary productivity of phytoplankton and Pyropia blades was $50,632kg\;C\;d^{-1}$. The primary productivity of Pyropia blades was 3.5 times greater than that of phytoplankton in the Manho region on the southwestern coast of Korea.

NLRC4 Inflammasome-Mediated Regulation of Eosinophilic Functions

  • Ilgin Akkaya;Ece Oylumlu;Irem Ozel;Goksu Uzel;Lubeyne Durmus;Ceren Ciraci
    • IMMUNE NETWORK
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    • v.21 no.6
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    • pp.42.1-42.20
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    • 2021
  • Eosinophils play critical roles in the maintenance of homeostasis in innate and adaptive immunity. Although primarily known for their roles in parasitic infections and the development of Th2 cell responses, eosinophils also play complex roles in other immune responses ranging from anti-inflammation to defense against viral and bacterial infections. However, the contributions of pattern recognition receptors in general, and NOD-like receptors (NLRs) in particular, to eosinophil involvement in these immune responses remain relatively underappreciated. Our in vivo studies demonstrated that NLRC4 deficient mice had a decreased number of eosinophils and impaired Th2 responses after induction of an allergic airway disease model. Our in vitro data, utilizing human eosinophilic EoL-1 cells, suggested that TLR2 induction markedly induced pro-inflammatory responses and inflammasome forming NLRC4 and NLRP3. Moreover, activation by their specific ligands resulted in caspase-1 cleavage and mature IL-1β secretion. Interestingly, Th2 responses such as secretion of IL-5 and IL-13 decreased after transfection of EoL-1 cells with short interfering RNAs targeting human NLRC4. Specific induction of NLRC4 with PAM3CSK4 and flagellin upregulated the expression of IL-5 receptor and expression of Fc epsilon receptors (FcεR1α, FcεR2). Strikingly, activation of the NLRC4 inflammasome also promoted expression of the costimulatory receptor CD80 as well as expression of immunoregulatory receptors PD-L1 and Siglec-8. Concomitant with NLRC4 upregulation, we found an increase in expression and activation of matrix metalloproteinase (MMP)-9, but not MMP-2. Collectively, our results present new potential roles of NLRC4 in mediating a variety of eosinopilic functions.