• The Plant Pathology Journal
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• 제24권2호
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• pp.171-177
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• 2008

In this study, we investigated the induced defense response and protective effects against Stemphylium vesicarium by application of benzothiadiazole ($Bion^{(R)}$) and di-potassium phosphate salt $(K_2HPO_4)$ to onion. Onion leaves were sprayed with $Bion^{(R)}$ and $K_2HPO_4$, then inoculated 2 days later with a virulent strain of S. vesicarium under greenhouse conditions. Disease severity and activities of peroxidase (PO), polyphenoloxidase, phenylalanine ammonia-lyase (PAL) and phenol contents were evaluated in the treated leaf tissues. Reduction in the disease severity was observed in plants treated with $Bion^{(R)}$ and $K_2HPO_4$. Onion plants treated with $Bion^{(R)}$ and $K_2HPO_4$ and inoculated with the pathogen showed significantly higher PAL activity, PO activity, and phenol contents than inoculated water-treated plants 2 days after the treatment. In conclusion, the results of this study provide evidence that application of simple non-toxic chemical solutions as di-potassium phosphate and $Bion^{(R)}$ can control Stemphylium leaf blight of onion.

• 동의신경정신과학회지
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• 제19권2호
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• pp.65-75
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• 2008

Objective : This experiment was designed to investigate the effect of the KBHT and PMCMT extract on protecting microglia and inhibiting acetylcholinesterase and oxidants. Method : The effects of the KBHT and PMCMT extract on cell death of BV2 microglial cell line treated by ${\gamma}$ ; expression of NO, ROS in BV2 microglial cell line treated by lipopolysaccharide(LPS) ; AChE activity in PC-12 cell treated by NGF were investigated, respectively. Result : The KBHT and PMCMT extract significantly increased cell viability in BV2 microglial cell line treated with ${\gamma}$. The KBHT and PMCMT extract suppressed the NO and ROS production in BV2 microglial cell line treated by LPS. The KBHT and PMCMT extract groups also showed inhibition of AChE activity in PC-12 cell line. Conclusion : According to the above result, it is suggested that the KBHT and PMCMT extract might be usefully applied for prevention and treatment of Alzheimer' s disease.

• Nutrition Research and Practice
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• 제9권1호
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• pp.71-78
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• 2015

BACKGROUND/OBJECTIVES: The purposes of this study were to compare total energy expenditure (including PAL and RMR) of Korean farmers between the farming season and off farming season and to assess the accuracy of estimated energy requirement (EER) prediction equation reported in KDRIs. SUBJECTS/METHODS: Subjects were 72 Korean farmers (males 23, females 49) aged 30-64 years. Total energy expenditure was calculated by multiplying measured RMR by PAL. EER was calculated by using the prediction equation suggested in KDRIs 2010. RESULTS: The physical activity level (PAL) was significantly higher (P < 0.05) in the farming season (male $1.77{\pm}0.22$, female $1.69{\pm}0.24$) than the off farming season (male $1.53{\pm}0.32$, female $1.52{\pm}0.19$). But resting metabolic rate was significantly higher (P < 0.05) in the off farming season (male $1,890{\pm}233kcal/day$, female $1,446{\pm}140kcal/day$) compared to the farming season (male $1,727{\pm}163kcal/day$, female $1,356{\pm}164kcal/day$). TEE ($2,304{\pm}497kcal/day$) of females was significantly higher in the farming season than that ($2,183{\pm}389kcal/day$) of the off farming season, but in males, there was no significant difference between two seasons in TEE. On the other hand, EER of male and female ($2,825{\pm}354kcal/day$ and $2,115{\pm}293kcal/day$) of the farming season was significantly higher (P < 0.05) than those ($2,562{\pm}339kcal/day$ and $1,994{\pm}224kcal/day$) of the off farming season. CONCLUSIONS: This study indicates that there is a significant difference in PAL and TEE of farmers between farming and off farming seasons. And EER prediction equation proposed by KDRI 2010 underestimated TEE, thus EER prediction equation for farmers should be reviewed.

• Journal of Electrochemical Science and Technology
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• 제7권4호
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• pp.298-305
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• 2016

The development of non-precious metal based electrocatalysts is highly desired for the oxygen reduction reaction (ORR) as alternates to noble metal based ORR electrocatalysts. Herein, we report mononulcear copper(II) complex $[CuLbpy]ClO_4$ (L=4-[(2-hydroxyphenylimino)methyl]benzoic acid) containing poly(allylamine.HCl) polymer (PAlACuLbpy) as an electrocatalyst for oxygen reduction reaction (ORR). PAlACuLbpy was mixed with poly(acrylic acid) and tetraethylortho silicate to prepare a composite and then deposited on the screen printed electrode surface. The modified electrode (PAlACuLbpy/PCE) is highly stable and showed a quasi-reversible redox behavior with $E_{1/2}=-0.2V$ vs. Ag/AgCl(3 M KCl) in 0.1 M phosphate buffer at pH 7 under argon atmosphere. PAlACuLbpy/PCE exhibited a remarkable ORR activity with an onset potential of -0.1 V vs Ag/AgCl in 0.1 M PB (pH 7) in the presence of oxygen. The kinetics for ORR was studied by rotating disk voltammetry in neutral aqueous medium and the results indicated that the number of electrons involving in the ORR is four and the conversion products are water and hydrogen peroxide.

• Natural Product Sciences
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• 제3권1호
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• pp.1-7
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• 1997

Eucalyptus is a rich source of biologically active compounds. Among these, phloroglucinol compounds such as sideroxylonals, macrocarpals, euglobals, and robustadials are unique to Eucalyptus species. Sideroxylonal A is a very potent attachment-inhibitor. Macrocarpals show very strong antibacterial activity against gram positive bacteria. Macrocarpals also show HIV-RTase inhibitory activity. Euglobals are potent inhibitors of Epstein-Barr virus activation and are developed as skin and antitumor agents. They also show granulation inhibitory activity. In this review we aim to remove the existing confusion in literature on macrocarpals and discuss the biological activities and structure-activity relationships of phloroglucinol compounds.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.233-237
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• 2000

As part of a continuing investigation to identify free radical scavengers from the fruit bodies of basidiomycetes, we isolated two p-terphenyl compounds, designated as PAl and PA2l, from cethanolic extract of the fruit body of Pasillus panuoides. The methanolic extract was processed by ethyl acetate extraction and silica gel column chromatography to yield two active fraction. PAl was obtained from one of the fractions through Sephadex LH-20 and silica gel column chromatographies and reverse-phase HPLC. The other fraction was purified by Sephadex LH-20 and reverse-phase column chromatographies to produce PA2. The compounds PA1 and PA2 were identified as leucomentin-4 and leucomentin-2, respectively, on the basis of various spectroscopic analyses. These compounds exhibited strong inhibitory activities against lipid peroxidation in rat liver microsomes with IC_{50}$ values of 0.10 and $0.06{\;}\mu\textrm{g}ml$, respectively.

• Archives of Pharmacal Research
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• 제19권3호
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• pp.219-222
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• 1996

Ephedra olistachya cultures have been known to accumulate $rho-coumaroylamino$ acids by elicitor treatment. Based on their chemical structures, the biosynthetic pathway of$rho-coumaroylamino$acids was postulated and phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (4-CH) and p-coumaroyl CoA: D-Ala p-coumaroyltransferase ($rho-CT$) were supposed to be involved in the pathway. The time course inductions of these enzymes were investigated after treatment of yeast extract, yeast-derived mannan glycopeptide and D-Ala. They were detectable at only 4 hours and reached to their maximum level at 9 hours after onset of elicitor treatment. The activities of PAL and 4-CH were almost disappeared within 24 hours, however, that of $rho-CT$was remained up to 48 hours irrespective of the kind of elicitors. $rho-CT$ showed substrate specificity to D-Ala at crude enzyme extract level.

• The Korean Journal of Physiology
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• 제5권1호
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• pp.19-22
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• 1971

The effect based upon changes of body weight and effect of X-ray irradiation on tryptophan pyrrolase (TPO) activity in liver homogenate of albino rats was studied. 1. The average TPO activity of control group of 33 rats weighing $95{\sim}172g$\;was\;2.372{\pm}0.165{\mu}$ moles kynurenine/g protein/hr. 2. Correlation between body weight of 33 rats and its TPO activity was not showed practically(r=0.011). 3. TPO activity of whole-body X-ray irradiated rats (700r) was increased about double at 3hours and increased for a period of 4 days after irradiation, but after 6 days it was decreased gradually. This result is that whole-body X-ray irradiation showed dual effect on TPO activity in rat liver.

• The Plant Pathology Journal
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• 제34권5호
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• pp.403-411
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• 2018

This study takes strawberry-fruits as the test material and discusses the effect of Burkholderia contaminans B-1 on preventing postharvest diseases and inducing resistance-related substances in strawberry-fruits. Soaking and wound inoculating is performed to analyze the inhibitory effects of different treatment solutions on the gray mold of postharvest strawberry-fruits. The count of antagonistic bacteria colonies in the wound is found, and the dynamic growth of antagonistic bacteria and the pathogenic fungus is observed by electron microscopy. The results indicated that, either by soaking/wound-inoculating, the fermentation and suspension of antagonistic bacteria significantly reduced the incidence of postharvest diseases of strawberry-fruits. With wound inoculation, the inhibition rate of antagonist fermentation and suspension ($1{\times}10^{10}cfu/ml$) respectively reached 77.4% and 66.7%. It also led to a significant increase in the activity of resistance-related enzymes, i.e., phenylalanine ammonia lyase (PAL), 4-coumarate coenzyme A ligase (4CL), cinnamate-4-hydroxylase (C4H) and chalcone isomerase (CHI). On 1 d and 2 d post-treatment, the activity of 4CL was respectively 3.78 and 6.1 times of the control, and on 5 d, the activity of PAL was increased by 4.47 times the control. The treatment of antagonistic bacteria delayed the peaking of cinnamyl-alcohol dehydrogenase (CAD) activity and promoted the accumulation of lignin and total phenols. The antagonistic bacteria could be well colonized in the wounds. On 4-5 d post-inoculation, the count of colonies was $10^8$ times of that upon inoculation. Electronmicroscopy indicated that the antagonistic bacteria delayed the germination of pathogenic spores in the wounds, and inhibited further elongations of the mycelia.

• 한국산학기술학회논문지
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• 제9권6호
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• pp.1869-1873
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• 2008

본 연구는 가속도 센서를 이용하여 정신지체유아와 일반유아의 신체활동량을 비교 분석하는데 그 목적이 있었다. 연구대상은 정신지체유아 3명과 일반유아 3명을 대상으로 신체활동량을 분석하였다. 신체활동량의 계측은 횡축 가속도 최고치, 종축 가속도 최고치, 평균 열발산, 분당 보행 수, 피부 전도도이다. 정신지체유아를 교육하는 기관에서의 놀이활동 시간을 측정한 결과 횡축으로 움직였을 때($2.94{\pm}0.60$)와 종축으로 움직였을 때($4.97{\pm}0.65$) 일반유아들(횡측 종측 각각 $4.50{\pm}0.95$, $6.05{\pm}0.87$)보다 덜 움직이는 결과를 얻었다. 이는 신체적 일반 특성에서 운동능력이 부족함을 나타낸 것이다. 또한 평균 열발산과 분당보행수, 피부전도도가 일반 유아들보다 적게 나타났다. 이런 결과를 바탕으로 정신지체유아들의 교육에 있어서 정신지체유아들이 좀 더 흥미를 갖고 활동을 많이 그리고 빨리하도록 하는 교육 프로그램의 개발과 도입이 필요하다는 결론을 얻었다.