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Histopathological observation of the gill of the crucian carp, Carassius auratus by the leech, Limnotrachelobdella sinensis (거머리, Limnotrachelobdella sinensis의 기생으로 인한 붕어, Carassius auratus 아가미의 조직병리학적 관찰)

  • Park, Myoung-Ae;Kim, Seok-Ryel;Kim, Myoung-Sug;Kim, Jeong-Ho;Park, Jung-Jun
    • Journal of fish pathology
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    • v.23 no.3
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    • pp.399-407
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    • 2010
  • On the inner side of each operculum of the crucian carp, Carassius auratus (n=10), the leech, Limnotrachelobdella sinensis of 1-4 individuals were parasitic. The leeches had approximately 41.0 mm in total length and 11 mm in width. These body was composed with anterior sucker, neck, trunk and posterior sucker and average length was 2.3 mm, 7.2 mm, 23.3 mm and 8.7 mm respectively. To both sides of the trunk lateral vesicle of 11 pair existed. When observed by SEM, anterior sucker was hemisphere shape and the mouth where proboscis comes out existed with the its center. Proboscis was connected the esophagus directly. Under light microscopy, bloodsucking gill of C. auratus showed lamella fusion, hypertrophy the epithelial cell of the filament and lamella, increased mucocytes and congested capillaries. On the other hand, necrotic and hydropic degeneration epithelial cell of the lamella, and infiltration of the macrophages from some individuals were suggested the secondary infection with the bacteria or virus after bloodsucking activity of the leech.

Use of Microsatellite Markers Derived from Genomic and Expressed Sequence Tag (EST) Data to Identify Commercial Watermelon Cultivars (수박 시판 품종의 식별을 위한 Genomic과 Expressed Sequence Tag (EST)에서 유래된 Microsatellite Marker의 이용)

  • Kwon, Yong-Sham;Hong, Jee-Hwa;Kim, Du-Hyun;Kim, Do-Hoon
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.737-750
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    • 2015
  • This study was carried out to construct a DNA profile database for 102 watermelon cultivars through the comparison of polymorphism level and genetic relatedness using genomic microsatellite (gMS) and expressed sequence tag (EST)-microsatellite (eMS) markers. Sixteen gMS and 10 eMS primers showed hyper-variability and were able to represent the genetic variation within 102 watermelon cultivars. With gMS markers, an average of 3.63 alleles per marker were detected with a polymorphism information content (PIC) value of 0.479, whereas with eMS markers, the average number of alleles per marker was 2.50 and the PIC value was 0.425, indicating that eMS detects a lower polymorphism level compared to gMS. Cluster analysis and Jaccard's genetic distance coefficients using the unweighted pair group method with arithmetic average (UPGMA) based on the gMS, eMS, and combined data sets showed that 102 commercial watermelon cultivars could be categorized into 6 to 8 major groups corresponding to phenotypic traits. Moreover, this method was sufficient to identify 78 out of 102 cultivars. Correlation analysis with Mantel tests for those clusters using 3 data sets showed high correlation ($r{\geq}0.80$). Therefore, the microsatellite markers used in this study may serve as a useful tool for germplasm evaluation, genetic purity assessment, and fingerprinting of watermelon cultivars.

IDENTIFICATION OF PORPHYROMONAS ENDODONTALIS USING POLYMERASE CHAIN REACTION(RCR) (중합효소연쇄반응(Polymerase Chain Reaction)을 이용한 Porphyromonas endodontalis의 동정에 대한 연구)

  • Lee, Sang-Yup;Yoon, Soo-Han
    • Restorative Dentistry and Endodontics
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    • v.23 no.1
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    • pp.328-338
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    • 1998
  • Porphyromonas endodontalis, an anaerobic Gram negative cocobacillus which was known to be associated with the infected root canals and periapical lesions, is very difficult to culture and to detect by the traditional method in that it requires much time to induce the specific black pigmentation, and it is very sensitive to oxygen and the antibiotics added in the culture medium. In this study, the nucleotide sequences of the 'probe h' (0.73kb), one of the specific DNA probes top. endodontalis (ATCC 35406) which had been developed by our department, was determined and then a pair of primers for PCR amplification was fabricated to identify P. endodontalis. The plasmids containing 'probe h' were purified by $Wizard^{TM}$ Midipreps DNA Purification System (Promega Corp.), and the nucleotide sequences of the 'probe h' were determined by the dideoxy chain termination method using TaqTrack Sequencing System (Promega Corp.) and detected by fluorescent labelling method. The sense/antisense PCR primers were designed with computer software (Lasergene, DNASTAR Ind. PCR was done with a programmable GeneAmp PCR System 2400 (Perkin Elmer-Cetus Co.). Each sample containing the whole genomic DNA of P. endodontalis and other black-pigmented Bacteroides was itailly denatured at $94^{\circ}C$ for 5 min and then subjected to 30 cycles, each of them consisting of 60s at $94^{\circ}C$, 60s at $60^{\circ}C$, and 90s. at $72^{\circ}C$. The amplified DNA was resolved electrophoretically in a 1.0 % agarose gel in 1X TAE buffer, stained with EtBr, and photographed on a UV transilluminator. The results were as follows : 1. The nucleotide sequences of 'probe h' (743 base pairs) were obtained by dideoxy chain termination method, and from that results the specific primers to P. endodontalis (ATCC 35406), 'Primer H1/ Primer H2', were designed. 2. It has been found that 'Primer H1/H2' could detect P. endodontalis (ATCC 35406) using PCR. 3. The PCR system with this primers may be a powerful technique to amplify the specific sequences of 'probe h' of P. endodontalis (ATCC 35406) that produce distinct identification of it from other black-pigmented Bacteroides, and this could help us to determine the nature of periapical disease.

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Supporting RSVP for IP Multicast over ATM Networks with MARS Architecture based on MCS (MCS 기반 MARS를 사용하는 ATM 망에서의 IP 멀티캐스트를 위한 RSVP 지원 방안)

  • Choe, Jeong-Hyeon;Lee, Mi-Jeong
    • Journal of KIISE:Computer Systems and Theory
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    • v.26 no.7
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    • pp.813-826
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    • 1999
  • 실시간 멀티미디어 응용의 등장으로 멀티캐스트와 QoS(Quality of Service) 지원이 필수적인 망 서비스로 부각되고 있다. 이에, ATM 기반의 인터넷에서 IP 멀티캐스트의 효율적인 처리를 위하여 MARS(Multicast Address Resolution Server)가 제안되었고, 기존의 최선 서비스 기반의 인터넷에서 QoS(Quality of Service)를 지원하기 위하여 RSVP(Resource Reservation Protocol)가 제안되었다. 본 논문에서는 ATM 망에서 QoS가 지원되는 IP 멀티캐스트 서비스를 제공하기 위하여 MARS 구조에서 RSVP를 지원하는 두 가지 방안을 제안하고, 시뮬레이션을 통하여 그 성능을 분석하였다. 제안하는 두 가지 방법은 각각 'RSVP 전 홉 노드 방식'과 'MARS 서버 방식'이라 명명하였다. RSVP 전 홉 노드 방식은 송신원으로부터 ATM 망으로 진입하는 노드와 수신원을 향하여 ATM 망을 진출하는 노드 간에 각각 일대일 양방향 VC를 설정하여 멀티캐스트 그룹에 속하는 수신원들이 보내는 자원 예약 메시지를 ATM 망에서 전송하는 방안이다. MARS 서버 방식은 ATM 망을 진출하는 노드와 MARS 서버간에 MARS 제어 메시지 교환을 위해 존재하는 ATM VC를 사용하여 RSVP의 자원 예약 메시지를 전송하고, MARS 서버가 RSVP 자원 예약 메시지를 처리하도록 그 기능을 확장함으로써 ATM 망에서 필요로 하는 제어 VC 수를 절약할 수 있는 방안이다. 시뮬레이션을 통하여, MARS 서버 방식은 ATM 제어 VC의 수를 절약할 수 있을 뿐 아니라 경우에 따라 RSVP 자원 예약 메시지 전달 지연을 줄일 수도 있음을 볼 수 있었다. 그러나, MARS 클러스터 내에 동시에 존재하는 RSVP 흐름이 많을 때에는 MARS 서버 방식의 경우 MARS 서버에서의 병목 현상으로 인해 성능이 저하될 수 있다.Abstract Emerging real time multimedia applications require multicast service with a QoS(Quality of Service) support. An overlay service architecture MARS(Multicast Address Resolution Server) is proposed to support IP multicast over an ATM network, and a resource reservation protocol RSVP is proposed to provide QoS support in the Internet which is originally based upon best effort service only. In this paper, we propose two schemes to support IP multicast service with QoS support over ATM networks: 'RSVP Previous Hop Node(RPHN) scheme' and 'MARS server based scheme'. In RPHN scheme, the RSVP reservation messages are transported via one-to-one ATM control VC from the egress nodes to the ingress nodes of the the multicast flow set up between each pair of nodes. The RSVP message processing occurs at the ingress nodes of the multicast flow. Whereas, in the MARS server based scheme, the RSVP reservation messages are transported via the MARS control VCs between the egress nodes and the MARS server. The RSVP message processing burden is imposed at MARS server in this scheme. For MARS server based scheme, no additional ATM VC is required for RSVP reservation message transmission, while the processing burden at the MARS server is high. Simulation results show that the MARS server based scheme, may accomplish RSVP reservation message delivery with smaller delay as well as saving of the number of ATM VCs. When the number of simultaneous RSVP flows in the MARS cluster is large, however, MARS based scheme may suffer performance degradation since MARS server becomes a performance bottleneck.

A CDMA Network-based Wireless System for Measuring Lap Time on a Ski Slope (CDMA 망에 기반한 스키장 슬로프의 무선 구간 기록 측정 시스템)

  • Lee, Hyung-Bong;Park, Lae-Jeong;Moon, Jung-Ho;Chung, Tae-Yun
    • The KIPS Transactions:PartD
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    • v.16D no.1
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    • pp.133-138
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    • 2009
  • This paper introduces a pilot CDMA network-based wireless lap time measurement system set up on a ski slope of Yongpyong Ski Resort. The wireless lap time measurement system is one output of U-Sports Project of Gangwon Province, which is intendended for promoting local strategic business and preparation for hosting 2018 Winter Olympic Games at Pyeongchang. A pair of laser sensors is installed at the entry and exit points of a section requiring lap time measurement on a ski slope. Each laser sensor is connected to a sensor node via wire so that the sensor node can detect the time when a skier enters or exits the section. Also each sensor node is connected to a CDMA network via a modem and receives a standard time from a NTP server. Each node executes the NTP algorithm to synchronize its local time to the received server time. As a result of the time synchronization, the sensor nodes maintain its local time within a resolution of at least 10 miliseconds and transmit the time of detection to a central control center. While the wireless lap time measurement system introduced in the paper does not need expensive measurement equipment, the system allows the central control center to provide lap time records in a more convenient manner compared to conventional manual lap time measuremnt methods.

A Comparative Experiment on Dimensional Reduction Methods Applicable for Dissimilarity-Based Classifications (비유사도-기반 분류를 위한 차원 축소방법의 비교 실험)

  • Kim, Sang-Woon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.3
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    • pp.59-66
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    • 2016
  • This paper presents an empirical evaluation on dimensionality reduction strategies by which dissimilarity-based classifications (DBC) can be implemented efficiently. In DBC, classification is not based on feature measurements of individual objects (a set of attributes), but rather on a suitable dissimilarity measure among the individual objects (pair-wise object comparisons). One problem of DBC is the high dimensionality of the dissimilarity space when a lots of objects are treated. To address this issue, two kinds of solutions have been proposed in the literature: prototype selection (PS)-based methods and dimension reduction (DR)-based methods. In this paper, instead of utilizing the PS-based or DR-based methods, a way of performing DBC in Eigen spaces (ES) is considered and empirically compared. In ES-based DBC, classifications are performed as follows: first, a set of principal eigenvectors is extracted from the training data set using a principal component analysis; second, an Eigen space is expanded using a subset of the extracted and selected Eigen vectors; third, after measuring distances among the projected objects in the Eigen space using $l_p$-norms as the dissimilarity, classification is performed. The experimental results, which are obtained using the nearest neighbor rule with artificial and real-life benchmark data sets, demonstrate that when the dimensionality of the Eigen spaces has been selected appropriately, compared to the PS-based and DR-based methods, the performance of the ES-based DBC can be improved in terms of the classification accuracy.

Partial versus Radical Nephrectomy for T1-T2 Renal Cell Carcinoma in Patients with Chronic Kidney Disease Stage III: a Multiinstitutional Analysis of Kidney Function and Survival Rate

  • Chung, Jae-Seung;Son, Nak Hoon;Lee, Sang Eun;Hong, Sung Kyu;Jeong, Chang Wook;Kwak, Cheol;Kim, Hyeon Hoe;Hong, Sung Hoo;Kim, Yong June;Kang, Seok Ho;Chung, Jinsoo;Kwon, Tae Gyun;Hwang, Eu Chang;Byun, Seok-Soo
    • Journal of Korean Medical Science
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    • v.33 no.43
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    • pp.277.1-277.10
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    • 2018
  • Background: To examine survival rates and renal function after partial nephrectomy (PN) and radical nephrectomy (RN) in patients with chronic kidney disease (CKD). Methods: We studied 4,332 patients who underwent PN or RN for pathological T1a-T2N0M0 renal cell carcinoma from 1988 to 2014. Patients were divided into two subgroups of CKD stage I-II and stage III. Kidney function, and survival outcomes were compared between groups. Results: We included 1,756 patients with CKD I-II and 276 patients with CKD III in the final pair-matched analysis. Kidney function was significantly better preserved in the PN than in the RN group among all patients. However, the beneficial effect of PN on kidney function gradually disappeared over time in CKD III patients. The 5-year overall survival (OS) rates after PN and RN differed in patients with CKD I-II disease (99.4% vs. 96.5%, respectively, P = 0.015). The 5-year OS rates after surgery were not affected by mode of nephrectomy in CKD III patients (97.8% vs. 93.5%, P = 0.103). The 5-year cancer-specific survival rates did not differ between treatment groups in all CKD stage. Cox hazard analysis showed that the operative method was a significant factor for OS in CKD I-II patients (hazard ratio [HR], 0.320; confidence interval [CI], 0.122-0.840; P = 0.021). However, PN was not beneficial in terms of OS in CKD III patients (HR, 0.395; CI, 0.086-1.172; P = 0.117). Conclusion: PN is associated with a higher OS rate and better kidney function in patients with preoperative CKD stage I and II, but not in those with CKD stage III.

A Study on the Enhancement of DEM Resolution by Radar Interferometry (레이더 간섭기법을 이용한 수치고도모델 해상도 향상에 관한 연구)

  • Kim Chang-Oh;Kim Sang-Wan;Lee Dong-Cheon;Lee Yong-Wook;Kim Jeong Woo
    • Korean Journal of Remote Sensing
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    • v.21 no.4
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    • pp.287-302
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    • 2005
  • Digital Elevation Models (DEMs) were generated by ERS-l/2 and JERS-1 SAR interferometry in Daejon area, Korea. The quality of the DEM's was evaluated by the Ground Control Points (GCPs) in city area where GCPs were determined by GPS surveys, while in the mountain area with no GCPs, a 1:25,000 digital map was used. In order to minimize errors due to the inaccurate satellite orbit information and the phase unwrapping procedure, a Differential InSAR (DInSAR) was implemented in addition to the traditional InSAR analysis for DEM generation. In addition, DEMs from GTOPO30, SRTM-3, and 1:25,000 digital map were used for assessment the resolution of the DEM generated from DInSAR. 5-6 meters of elevation errors were found in the flat area regardless of the usage and the resolution of DEM, as a result of InSAR analyzing with a pair of ERS tandem and 6 pairs of JERS-1 interferograms. In the mountain area, however, DInSAR with DEMs from SRTM-3 and the digital map was found to be very effective to reduce errors due to phase unwrapping procedure. Also errors due to low signal-to-noise ratio of radar images and atmospheric effect were attenuated in the DEMs generated from the stacking of 6 pairs of JERS-1. SAR interferometry with multiple pairs of SAR interferogram with low resolution DEM can be effectively used to enhance the resolution of DEM in terms of data processing time and cost.

Characterization of Physical Processes and Secondary Particle Generation in Radiation Dose Enhancement for Megavoltage X-rays (MV X선의 방사선 선량 증강 현상에서 물리적 특성과 이차입자의 발생)

  • Hwang, Chulhwan;Kim, JungHoon
    • Journal of the Korean Society of Radiology
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    • v.13 no.5
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    • pp.791-799
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    • 2019
  • We evaluated the physical properties that occur to dose enhancement and changes from secondary particle production resulting from the interaction between enhancement material. Geant4 was used to perform a Monte Carlo simulation, and the medical internal radiation dose (MIRD) head phantom were employed. X-rays of 4, 6, 10, 15, 18, and 25 MV were used. Aurum (Au) and gadolinium (Gd) were applied within the tumor volume at 10, 20, and 30 mg/g, and an experiment using soft tissue exclusively was concomitantly performed for comparison. Also, particle fluence and initial kinetic energy of secondary particle of interaction were measured to calculate equivalent doses using the radiation weight factor. The properties of physical interaction by the radiation enhancement material showed the great increased in photoelectric effect as compared to the compton scattering and pair production, occurred with the highest, in aurum and gadolinium it is shown in common. The photonuclear effect frequency increased as the energy increased, thereby increasing secondary particle production, including alpha particles, protons, and neutrons. During dose enhancement using aurum, a maximum 424.25-fold increase in the equivalent dose due to neutrons was observed. This study was Monte Carlo simulation corresponds to the physical process of energy transmission in dose enhancement. Its results may be used as a basis for future in vivo and in vitro experiments aiming to improve effects of dose enhancement.

Velocity Distribution Measurements in Mach 2.0 Supersonic Nozzle using Two-Color PIV Method (Two Color PIV 기법을 이용한 마하 2.0 초음속 노즐의 속도분포 측정)

  • 안규복;임성규;윤영빈
    • Journal of the Korean Society of Propulsion Engineers
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    • v.4 no.4
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    • pp.18-25
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    • 2000
  • A two-color particle image velocimetry (PIV) has been developed for measuring two dimensional velocity flowfields and applied to a Mach 2.0 supersonic nozzle. This technique is similar to a single-color PIV technique except that two different color laser beams are used to solve the directional ambiguity problem. A green-color laser sheet (532 nm: 2nd harmonic beam of YAG laser) and a red-color laser sheet (619 nm: output beam from YAG pumped Dye laser using Rhodamine 640) are employed to illuminate the seeded particles. A high resolution (3060${\times}$2036) digital color CCD camera is used to record the particle positions. This system eliminates the photographic-film processing time and subsequent digitization time as well as the complexities associated with conventional image shifting techniques for solving directional ambiguity problem. The two-color PIV also has the advantage that velocity distributions in high speed flowfields can be measured simply and accurately by varying the time interval between two different laser beams due to its high signal-to-noise ratio and thereby less requirement of panicle pair numbers for a velocity vector in one interrogation spot. The velocity distribution in the Mach 2.0 supersonic nozzle has been measured and the over-expanded shock cell structure can be predicted by the strain rate field. These results are compared and analyzed with the schlieren photograph for the velocity distributions and shock location.

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