거머리, Limnotrachelobdella sinensis의 기생으로 인한 붕어, Carassius auratus 아가미의 조직병리학적 관찰

Histopathological observation of the gill of the crucian carp, Carassius auratus by the leech, Limnotrachelobdella sinensis

  • 박명애 (국립수산과학원 병리연구과) ;
  • 김석렬 (국립수산과학원 병리연구과) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 김정호 (강릉원주대학교 해양자원육성) ;
  • 박정준 (국립수산과학원 병리연구과)
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Seok-Ryel (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Myoung-Sug (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Jeong-Ho (Faculty of Marine Bioscience and Technology Gangneung-Wonju Nat. Univ.) ;
  • Park, Jung-Jun (Pathology Division, National Fisheries Research and Development Institute)
  • 투고 : 2010.11.18
  • 심사 : 2010.11.22
  • 발행 : 2010.12.31

초록

10 개체의 붕어, Carassius auratus 아가미뚜껑의 한쪽면에 1-4개체의 거머리, Limnotrachelobdella sinensis들이 기생하고 있었다. 거머리들의 평균 길이는 약 41.0 mm 정도였고, 폭은 11 mm였다. 이들의몸체는 anterior sucker, neck, trunk, posterior sucker로 구성되어 있었고 각각의 평균 길이는 2.3 mm, 7.2 mm, 23.3 mm, 8.7 mm였다. 몸통의 양 옆에는 11쌍의 lateral vesicle이 존재하였다. Anterior sucker를 SEM으로 관찰한 결과 이들은 반구모양으로 중앙에는 proboscis가 나오는 입이 존재하고 있었다. Proboscis는 식도와 바로 연결되어 있었다. 거머리에 의해서 흡혈되어진 붕어의 아가미를 광학현미경으로 관찰하였을 때 새판융합, 새엽 및 새판 상피세포 과증식, 점액세포 증가 및 울혈이 관찰되었다. 하지만 몇몇 개체들에서 나타나는 새엽 상피세포의 괴사 및 수종변성과 식세포의 침투 등은 거머리의 흡혈 활동 이후 세균 혹은 바이러스에 의한 2차 감염이 원인인 것으로 생각되어진다.

On the inner side of each operculum of the crucian carp, Carassius auratus (n=10), the leech, Limnotrachelobdella sinensis of 1-4 individuals were parasitic. The leeches had approximately 41.0 mm in total length and 11 mm in width. These body was composed with anterior sucker, neck, trunk and posterior sucker and average length was 2.3 mm, 7.2 mm, 23.3 mm and 8.7 mm respectively. To both sides of the trunk lateral vesicle of 11 pair existed. When observed by SEM, anterior sucker was hemisphere shape and the mouth where proboscis comes out existed with the its center. Proboscis was connected the esophagus directly. Under light microscopy, bloodsucking gill of C. auratus showed lamella fusion, hypertrophy the epithelial cell of the filament and lamella, increased mucocytes and congested capillaries. On the other hand, necrotic and hydropic degeneration epithelial cell of the lamella, and infiltration of the macrophages from some individuals were suggested the secondary infection with the bacteria or virus after bloodsucking activity of the leech.

키워드

참고문헌

  1. Bhagwant, S. and Elahee, K.B.: Pathologic gill lesions in two edible laggon fish species,l Mulloidichthys flavolineatus and Mugil cephalus, from the bay of Poudre d'Or, Mauritius. Western Indian Ocean J. Mar. Sci., 1:35-42, 2002.
  2. Burreson, E.M. and Allen, M.D.: Morphology and biology of Mysidobdella borealis (Johansson) comb. n. (Hirudinea: Piscicolidae), from mysids in the western North Atlantic. J. Parasitol., 64:1082-1091, 1978. https://doi.org/10.2307/3279730
  3. Fredric R. Govedich, D.W. Blinn, P.K. and Ronald, W.D.: Phylogenetic relationships of three genera of Erpobdellidae (Hirudinoidea), with a description of a new genus, Motobdella, and species, Motobdella sedonensis. Can. J. Zool., 76(12):2164-2171, 1998.
  4. Gardner, G.R. and Yevich, P.P.: Histological and hematological responses of an estuarine teleost to cadmium. J. Fish. Res. Brd. Can., 27:2185-2196, 1970. https://doi.org/10.1139/f70-248
  5. Govedich, F.R. and Bain, B.A.: All about leeches. http://www.invertebrate.ws, 2005.
  6. Janssen, H.H.: Morphology, egg cocoons, and transmission paths of the Antarctic leech Glyptonotobdella antarctica Sawyer and White, 1969 (Hirudinea: Rhynchobdelliformes: Piscicolidae). Polar Biol., 13:347-354, 1993.
  7. Meyers, T.R. and Hendricks, J.D.: Histopathology. In Fundamentals of aquatic toxicology; Methods and applications, pp. 283-331, Rand, G.M. and Petrocelli, S.R., Hemisphere Publishing Corporation, New York, 1985.
  8. Moller, H. and Anders, K.: Disease and parasites of marine fishes, pp. 179-183, Verlag Moller, Kiel, 1986.
  9. Nagasawa, K., Park, S.-W., Kim, Y.-G. and Kim, H.J.: Limnotrachelobdella sinensis, a leech associated with mortality in a wild population of Japaneses curcian carp Carassius cuvieri in Korea. J. Grad. Sch. Biosp. Sci., 48:49-53, 2009.
  10. Nehili, M., Ilk, C., Mehlhorn, H., Ruhnau, K., Dick, W. and Mjayou, M.: Experiments on the possible role of leeches as vectors of animal and human pathogens: a light and electron microscopy study. Parasitol. Res., 80:277-290, 1994. https://doi.org/10.1007/BF02351867
  11. Ogawa, K., Rusinek, O. and Tanaka, M.: New record of the leech Limnotrachelobdella sinensis infecting freshwater fish from Japanese water. Fish Pathol., 42:85-89, 2007. https://doi.org/10.3147/jsfp.42.85
  12. Park, S.-W. and Kim, Y.-G.: The case report on the leech, Trachelobdella sp. infestation in wild crucian carp (Carassius cuvieri) of Chungnam province in Korea. J. Fish Pathol. 15:117-119, 2002.
  13. Rhee, J.K.: Trachelobdella sinensis Blanchard, 1986 found from Cyprinus carpio nudus in Korea. Korean J. Parasitol., 24:216-217, 1986. https://doi.org/10.3347/kjp.1986.24.2.216
  14. Skidmore, J.F. and Tovell, P.W.A.: Toxic effects of zinc sulphate on the gills of rainbow trout. W. Res. 6:271-230, 1972. https://doi.org/10.1016/0043-1354(72)90004-8
  15. Stachowitsch, M.: The invertebrates; An illustrated glossary, pp. 273-279, Wiley-Liss, New York, 1992.
  16. Takashima, F. and Hibiya, T.: V. Gills. In An atlas of fish histology: normal and pathological features, pp. 66-71, Kodaxnsha Ltd., Tokyo, 1995.
  17. Yang, T.: On the genus Limnotrachelobdella Epshtein, 1968 and a new species from south China sea. Acta Hydrobiol. Sin., 11:268-273, 1987.