• Korean Journal of Pharmacognosy
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• v.45 no.1
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• pp.28-34
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• 2014

Rhei Rhizoma (RR) and Moutan cortex (MC) have been reported to have anti-inflammatory effects. However, little is known about the effects of RR and MC on endothelial inflammation and insulin resistance (IR). This study aims to investigate whether the water extracts of RR and MC could exert protection against palmitic acid (PA)-induced inflammation and IR in human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated for 6 h with RR or MC, and then exposed to PA for 24 h. The levels of interleukin-6 (IL-6) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) were determined by enzyme-linked immunosorbant assay kits. Western blot analysis was performed for activation of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and insulin receptor substrate-1 (IRS-1). In HUVECs stimulated with PA, both RR and MC significantly inhibited the production of TNF-${\alpha}$ and IL-6 and the activation of NF-${\kappa}B$. At the same concentrations, the inhibitory effects of RR were more potent than those of MC. PA reduced insulin-induced phosphorylation of IRS-1, which was reversed by RR and MC. The results suggest that RR and MC are effective in inhibiting PA-associated endothelial inflammation and ameliorating IR by beneficial regulation of NF-${\kappa}B$ and IRS-1 activation.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.07a
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• pp.188-194
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• 2002

버섯의 감압건조시험을 할 수 있는 요인시험장치를 제작하여 시험한 결과를 요약하면 다음과 같다. 가. 댐퍼조절방식 감압건조장치로 건조시험한 결과, 표고버섯은 건조온도 5$0^{\circ}C$에서는 나타나 건조실 압력에 따른 건감률의 변화는 크게 나타나지 않았으나, 건조온도 6$0^{\circ}C$에서 건조실 내부압력이 990hPa일 때 4.57%/hr로 1,013hPa에 비해 높게 나타났으며, 색차면에서는 온도 5$0^{\circ}C$일 때 건조실 내부 압력이 낮아질수록 변화가 적은 것으로 나타났고, 수축률은 압력별로 거의 변화가 없었으나, 건조온도 6$0^{\circ}C$, 압력 990hPa에서 다른 조건에 비해 양호한 것으로 판단되었으나 건조실 내부에 공기분배가 일정하지 않고, 건조실 내부압력 강하성능이 떨어지는 문제점이 발생하여 솔레노이드를 이용한 압력조절방식으로 보완 제작하여 건조시험을 실시하였다. 나. 솔레노이드방식 감압건조장치로 건조시험 한 결과, 표고버섯은 열풍온도 5$0^{\circ}C$, 건조실 내부압력 940-980hPa에서 건감률 2.69%w.b./hr, 갓의 복원률 90.1%로 가장 높았고, 색차 및 수축률이 가장 낮은 것으로 나타났으며, 건조실 내부압력을 900-940hPa로 고정하고 열풍온도를 40, 50, 6$0^{\circ}C$로 표고버섯을 시험한 결과 건감률은 6$0^{\circ}C$일 때 가장 높았으나, 색차, 수축률, 복원률을 고려할 경우 열풍온도 5$0^{\circ}C$가 가장 적합한 것으로 판단된다. 다 솔레노이드방식 감압건조장치로 건조한 표고버섯으로 품위에 대한 유의성 검증결과, 표고버섯의 경우 온도별로는 색택과 복원률, 건조실 내부 압력별로는 수축률, 복원률에서 유의차가 있는 것으로 나타났다. 라. 본 연구에서 구명된 감압건조특성을 기초로 하여 배치식 감압건조기를 설계 제작에 활용하고자 한다.

• Journal of the Korea Institute of Building Construction
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• v.15 no.4
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• pp.383-389
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• 2015

The objective of this study is to develop mixture proportioning approach of crack controlled lightweight foamed concrete without using high-pressure steam curing processes, as an alternative to autoclaved lightweight concrete blocks (class 0.6 specified in KS). To control thermal cracks owing to hydration heat of cementitious materials, 30% ground granulated blast-furnace slag (GGBS) was used as a partial replacement of ordinary portland cement (OPC). Furthermore, polyvinyl alcohol (PVA) and polyamid (PA) fibers were added to improve the crack resistance of foamed concrete. The use of 30% GGBS reduced the peak value of hydration production rate measured from isothermal tests by 28% and the peak temperature of foamed concrete measured from semi-adiabatic hydration tests by 9%. Considering the compressive strength development, internal void structure, and flexural strength of the lightweight foamed concrete, the optimum addition amount of PVA or PA fibers could be recommended to be $0.6kg/m^3$, although PA fiber slightly preferred to PVA fiber in enhancing the flexural strength of foamed concrete.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.251-251
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• 2004

Plasminogen activators (PA)는 다수의 세포 형태에서 분비되는 것으로 알려진 serine protease이다. PA는 섬유소 용해, 배란, 유선 퇴화, 착상 및 수정 등 다양한 생리적인 과정에 관여한다. 본 연구는 난포액이 돼지 난자의 체외성숙에 미치는 영향을 검토하기 위하여, 다양한 조건하에서의 돼지 난자의 성숙과 난구세포-난자 복합체(Cumulus-Oocyte complexes: COCs) 또는 conditioned medium 내의 PA 활성을 검토하였다. 직경 2∼6m 난포로부터 COCs를 회수하여 일부는 난구세포를 제거하였다. (중략)

• Review of KIISC
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• v.20 no.6
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• pp.62-72
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• 2010

정보통신 기술 및 전자상거래의 발전으로 지급결제 서비스는 성장을 거듭하고 있으나, 그 중심에 있는 전자지급결제의 위험 역시 계속해서 발견되고 있다. 지급결제카드산업의 정보보호를 위해 설립된 PCI SSC 에서는 지급결제 어플리케이션 개발에 대한 보안 표준인 PCI PA-DSS를 통해서 소프트웨어 벤더가 안전한 개발을 수행하도록 요구하고 있으나, 준수 대상인 기업 관점에서 참조 가능한 정보가 절대적으로 부족한 상황이다. 본 논문에서는 관련 보안 표준과의 연계 및 소프트웨어 개발 생명 주기를 통한 개발 절차 관리 방안을 통해, 소프트웨어 벤더가 PCI PA-DSS를 효과적으로 준수할 수 있는 방안을 제안한다.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.15-20
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• 2006

EDTA is known to have bacteriocidal effect on Vibrio vulnificus, pathogen of septicemia by osmotic shock in seafoods. Attempts were made to elucidate the bacteriocidal effect of phytic acid (PA) as a substitute for EDTA against V. vulnificus and its inhibition effect on the septicemia, which induces liver damage of the mice by the pathogen. Viable cells of V. vulnificus with the initial titre of $1.7{\times}10^6$ c.f.u. $ml^{-1}$ decreased by 90.6% after 1 min and 99.6% after 5 min in distilled water. The titre decreased by 65.9% and 94.5% in 2 mM solution of $Mg^{2+}$. In 0.1 mM solution of PA, the rate of decrease in titre was 97.4% after 1 min of incubation and 99.8% after 5 min, compared to 95.7% and 99.8% in 0.1 mM solution of EDTA. The bacteriocidal effect of PA solution at a concentration of 1 mM was marked: the rate of decrease in titre was 99.9% after 1 min. In relation to the bacteriocidal effect, PA was evaluated as a potential therapeutic agent for V. vulnificus septicemia in mouse. When the survival periods of mice were investigated by PA and EDTA treatment after the pathogen injection, the group of mice which infected by a low concentration of the strain survived longer than that inoculated at high concentration; also, the ratio of survival was 1.3 times higher in PA than in EDTA, showing that the fatal rate depended on the inoculation concentration. Although survival periods of mice induced with liver damage by carbon tetrachloride and then inoculated with the strain showed a similar trend, the fatal rate of mice was 2 times faster than those inoculated with only pathogen into normal liver, These results indicate that the infection by V. vulnificus was more fatal to those with liver disease. Also, symptoms of hemorrhage and inflammation on the mice with induced liver damage were reduced in case there was phytic acid treatment at each concentration.

• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.97-104
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• 2008

Plasminogen activators (PAs) are serine protease that cleave plasminogen to form the active protease plasmin. PA/plasmin system playa role in mammalian fertilization and motility and acrosome reaction of sperm. The present study was undertaken to identify PAs in porcine gametes and investigate a possible role of plasminogen in in vitro fertilization in the pig. When boar spermatozoa were preincubated in a fertilization medium (mTBM) for 0, 2, 4 or 6 h, the activity of tPA-PAI ($110{\sim}117\;kDa$), tPA ($62{\sim}70\;kDa$), and uPA ($34{\sim}38\;kDa$) was observed in the sperm incubation medium and sperm sample. PA activities in the sperm incubation medium significantly (p<0.05) increased according to increasing incubation times, while PA activities in sperm significantly (p<0.05) decreased at the same times. In addition, the rate of acrosome reaction in spermatozoa increased by increasing culture times. When oocytes were separated from porcine cumulus-oocytes complexes at 0, 22 or 44 h of maturation culture, no PA activities were observed in cumulus free-oocyte just after aspiration from follicles. However, the activity of tPA-PAI ($108{\sim}113\;kDa$) and tPA ($75{\sim}83\;kDa$) was observed at 22 h of in vitro culture and significantly (p<0.05) increased as the duration of the culture increased. On the other hand, when porcine oocytes were activated by sperm penetration or calcium ionophore, plasminogen significantly (p<0.05) increased ZP dissolution time (sec) in activated oocytes by sperm penetration. These results suggest that supplementation of plasminogen to fertilization medium may playa positive role in the improvement of in vitro fertilization ability in the pig.

• Korean Journal of Agricultural Science
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• v.42 no.4
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• pp.299-304
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• 2015

This study was conducted to investigate growth characteristics to develop the technique to select resistant cultivar by wet injury at an early stage through the automatic irrigation maintaining 30, 20, 10 kPa respectively using native variety, Chunpoong, Yunpoong, Gumpoong and Sunun. The aerial growth was decreased at 10 kPa compared to 30 kPa. In addition, the survival rate was decreased by 66.6%, 62.3%, 33.8% at 30, 20, 10 kPa, respectively. The survival rate of Chunpoong and Gumpoong were higher than others at 10 kPa. While root growth characteristics such as root length, root weight, number of lateral root and side root were tended to decrease, root diam was no significant or increased. And the more humid condition is, the more the incidence rate of rusty root and rough skin were tended to increase. The epidermal thickness of Chunpoong and Gumpoong was increased but the figures of native variety, Yunpoong and Sunun were decreased at 10 kPa compared to 30 kPa. But, the tissue stiffness of root was decreased at 10 kPa compared to 30 kPa.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.148-148
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• 2013

스퍼터 이온펌프(Sputter Ion Pump)는 주로 화학흡착으로 동작하며 기계적 진동이 없고, 기름 등의 오염 물질을 배출하지 않으며, 수명이 길어 초고청정 진공이 요구되는 표면실험장치, 표면분석계, 입자가속기 등에서 널리 사용 되고 있다. 일정한 지름을 갖는 다수의 원통 양극과 그 양단에 두개의 음극판을 배치시킨 후, 양극과 음극 사이에 수 kV의 전압을 걸고 원통의 축방향으로 자장을 인가하면 페닝 방전이 발생한다. 냉음극에서 방출된 전자는 양극으로 비행하면서 가스를 이온화한다. 이온분자는 가스흡수성 게터재료로 된 음극에 충돌하여 스퍼터링을 일으키며 게터막를 주변에 증착시킨다. 이온 및 중성 가스는 게터 고체막 속에 주입 포획되는 형태로 배기된다. 스퍼터 이온펌프는 $10^{-5}$ Pa 부근에서 최대 배기속도를 가지며, 압력이 낮아질 수록, 특히 $10^{-10}$ Pa영역 이하에서는 그 배기속도가 급격히 저하되며, $10^{-10}$ Pa영역에서는 배기능력을 거의 상실한다. 따라서 스퍼터 이온펌프 단독으로 진공시스템을 배기할 때 도달압력은 $10^{-9}$ Pa 영역에 머무르게 되며, $10^{-10}$ Pa 이하의 극고진공을 얻기 위해서는, $10^{-8}$ Pa 이하의 압력에서 배기 속도가 압력과 무관한 흡착펌프(getter pump)와 이온펌프를 조합하여 사용한다. 본 실험에서는 $600^{\circ}C$ 이상의 온도로 진공로에서 탈개스시킨 진공용기를 배기속도 450, 60, 30, 20, 5, 3 l/s의 6종류의 이온펌프와 배기속도 400 l/s, 100 l/s의 non-evaporable getter (NEG) 펌프를 조합시켜 배기하여 그 배기 특성을 비교하였다. 도달 압력은 이온펌프의 배기속도가 클수록 낮아지는 경향을 보여주었다. 450 l/s 이온펌프와 400 l/s NEG를 조합하여 배기시킬 때 도달 압력은 ~$2{\times}10^{-10}$ Pa을 기록하여 가장 낮았으며, 3 l/s 이온펌프와 400 l/s NEG를 조합하였을 때는 $ 2{\sim}3{\times}10^{-8}$ Pa을 기록하였다. 450 l/s 이온펌프와 400 l/s NEG를 조합한 경우 잔류가스의 대부분이 수소였으나, 3 l/s 이온펌프와 400 l/s NEG의 조합한 경우에는 메탄의 잔류량이 수소 보다 많았다. 이 결과는 메탄을 배기하지 못하는 NEG의 배기 특성을 보완하기 위해서는 일정 배기속도 이상의 이온 펌프가 필요함을 보여준다.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.218-225
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• 2022

High levels of proinflammatory cytokines have been observed in obese pregnancies. Obesity during pregnancy may increase the risk of various pregnancyrelated complications, with pathogenesis resulting from excessive inflammation. Palmitic acid (PA) is a saturated fatty acid that circulates in high levels in obese women. In our previous study, we found that PA inhibited the proliferation of trophoblasts developing into the placenta, induced apoptosis, and regulated the number of cleaved halves derived from transfer RNAs (tRNAs). However, it is not known how the expression of tRNA-derived stress-induced RNAs (tiRNAs) changes in response to PA treatment at concentrations that induce inflammation in human trophoblasts. We selected concentrations that did not affect cell viability after dose-dependent treatment of HTR8/SVneo cells, a human trophoblast cell line. PA (200 μM) did not affect the expression of apoptotic proteins in HTR8/SVneo cells. PA significantly increased the expression of inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α. In addition, 200 μM PA significantly increased the expression of tiRNAs compared to 800 μM PA treatment. These results suggest that PA impairs placental development during early pregnancy by inducing an inflammatory response in human trophoblasts. In addition, this study provides a basis for further research on the association between PA-induced inflammation and tiRNA generation.