• 한국방사선학회논문지
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• 제9권1호
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• pp.55-59
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• 2015

양성자핵반응에 대한 연구는 현재 핵융합로의 재료 개발을 비롯하여 양성자치료 분야 등을 중심으로 활발하게 이루어지고 있다. 본 연구는 100 MeV 양성자 빔을 이용한 $^{27}Al(p,3p+n)^{24}Na$ 반응을 통하여 발생되는 지발 감마선(2754, 1386 keV) 스펙트럼을 고순도 HPGe 검출기를 이용하여 측정하였다. 실험에 사용되어진 양성자 빔은 양성자가속기연구센터(KOMAC)에 설치되어 있는 100 MeV 양성자선형가속기를 사용하였다. 측정된 감마선은 기존에 알려진 결과들과 비교분석하였다. 측정된 감마선의 강도는 고에너지 감마선 검출효율을 결정하는데 매우 중요한 정도를 제공 할 것으로 생각되어 진다.

• 대한한방내과학회지
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• 제25권4호
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• pp.274-288
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• 2004

Objectives : This study was designed to evaluate the effect on cytotoxicity of Bojungikki-tang(BIT) in human lung cancer H460 cells. Methods : BIT-induced cell death was confirmed as apoptosis characterized by chromatin condensation and increase of the $sub-G_1$, DNA content. It was tested whether the water extract of BIT affects the cell cycle regulators such as, p2l/Cipl, p27/Kipl, cyclin $B_1$. Results : The data showed that treatment of BIT decreased the viability of H460 cells in a dose-dependent manner. p2l/Cip1 is gradually decreased by the addition of the cells with BIT extract. Interestingly, p27/Kip1 is not detected for 24 hr after the addition of BIT extract, however, after 24 hr, p27/Kipl markedly increased. In addition, cyclin $B_1$, decreased in a time dependent manner after the addition of the water extract. The activation of caspase -3 protease was further confirmed by degradation of procaspase-8 protease andpoly(ADP-ribose) polymerase(P ARP) by BIT in H460 cells. Moreover, BIT induced the increase of Bak expression. Conclusion : These results suggest that the extract of BIT exerts anticancer effects to induce the death of human lung cancer H460 cells via down regulation of cell cycle regulators such as p2l/Cip1, and cyclin B1 or up regulation of cell cycle regulators such as p27/Kip1. Moerover results suggest that BIT induces an apoptosis in H460 cells via activation of intrinsic caspase cascades.

• 한국식품영양과학회지
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• 제31권2호
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• pp.329-332
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• 2002

본 연구는 사람의 간암세포인 HepG2 세포를 대상으로 강력한 암 예방 효과물질을 함유하고 있을 것으로 추측되는 향버섯 메탄올 추출물의 암세포 성장 저해 효과를 검토하고 또한 암세포 성장 억제 효과의 분자생물학적 기전을 파악하기 위하여 암세포주의 세포주기 조절인자들의 발현을 조사하였다. 향버섯 메탄을 추출물의 HePG2세포에 대한 성장 저해 효과를 MTT assay로 검토한 결과 높은 암세포 성장 저해 효과를 나타내었으며 사람의 정상 간세포인 Chang cell에서는 세포독성이 나타나지 않았다. 또한 향버섯 추출물의 작용으로 HepG2 세포에서 cyclin A와 Dl 단백질의 발현이 억제 되었으며 cyclin Bl 단백질의 발현은 증가하는 경향을 나타내었다. 그리고 암 억제 단백질인 p53의 발현은 전반적으로 증가되었으며, 이와 대조적으로 PCNA 단백질은 감소하는 경향을 나타내었고 세포분열 억제 단백질 p27의 발현은 증가 하는 경향을 나타내었다. 이러한 결과로 볼 때 향버섯 메탄올 추출물은 간암세포의 세포주기 중 Gl기 에서 S기로의 진행을 조절하는 인자인 cyclin A와 cyclin Dl 발현을 억제시키고 p53, p27 단백질을 활성화 시킴과 동시에 PCNA 작용을 억제 함으로써 세포주기 중 Gl/S기 차단을 유도하여 암세포 증식을 억제한 것으로 추정된다.

• 한국축산식품학회지
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• 제41권2호
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• pp.343-352
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• 2021

The objective of this study was to evaluate the effects of Lactobacillus acidophilus ATCC 43121 and L. fermentum MF27 on biochemical indices in the serum, cholesterol metabolism in the liver and mucin expression in the gallbladder in lithogenic diet (LD)-induced C57BL/6J mice to determine the preventive effects of lactobacilli on gallstone formation. By the end of 4 wk of the experimental period, mice fed on a LD with high-fat and high-cholesterol exhibited higher levels of total and low-density lipoprotein cholesterol in the serum compared to mice fed on control diet or LD with L. acidophilus ATCC 43121 (LD+P1; p<0.05). Cholesterol-lowering effects observed in the LD+P1 and LD with L. fermentum MF27 (LD+P2) groups were associated with reduced expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver compared to the LD group (p<0.05). Furthermore, expression of the gel-forming mucin, including MUC5AB and MUC5B, was suppressed in the LD+P1 and LD+P2 groups compared to the LD group (p<0.05). Therefore, steady intake of both L. acidophilus ATCC 43121 and L. fermentum MF27 may have the ability to prevent the formation of cholesterol gallstones in LD-induced C57BL/6J mice.

• 한국응급구조학회지
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• 제27권1호
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• pp.91-100
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• 2023

Purpose: In patients with major trauma, mortality varies by age. This study aimed to identify predictors of death according to age. Methods: Data from the Community-Based Severe Trauma Survey in Korea were analyzed using a retrospective case-control design. Factors associated with death were identified by age using independent-samples t-tests, Welch's test, and χ² tests. Results: There were statistically significant differences in mortality by sex (p=.006), location (p=.029), mechanism of injury (MOI) (p<.001), intention (p<.001), transportation (p<.001), surgery (p<.001), and Injury Severity Score (ISS) (p<.001) in the ≤44 years age group; by location (p<.001), MOI (p=.004), intention (p<.001), transportation (p<.001), surgery (p<.001), and ISS (p<.001) in the 45-54 years age group; by location (p=.040), MOI (p<.001), transportation (p<.001), transfusion (p<.001), surgery (p<.001), and ISS (p<.001) in the 55-64 years age group; by location (p=.015), intention (p<.001), surgery (p<.001), and ISS (p<.001) in the 65-74 years age group; and by location (p=.002), intention (p<.001), transfusion (p=.020), surgery (p<.001), and ISS (p<.001) in the ≥75 years age group. Conclusion: In patients with major trauma, predictors of mortality varied by age.

• 한국환경생태학회지
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• 제31권2호
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• pp.135-151
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• 2017

2015년 10월부터 2016년 4월까지 주 1회 원주천의 조류를 관찰하여 각 수조류의 도래에 미치는 기온의 조건을 알고자 하였다. 원주천에 도래하는 수조류의 개체수는 기온의 변화와 반비례하여 기온이 낮아짐에 따라 개체수는 증가하였고, 최저기온을 보인 16차(27-Jan) 조사에서 최고개체수를 기록하였다가 기온이 상승함에 따라 감소하였다. 원주천 수조류의 전체 개체수는 인근 저수지와 하천의 결빙율에 비례하여 결빙율이 높아질수록 개체수가 증가하였고 낮아질수록 감소하였다. 기온변화에 따른 종별 개체수의 상관성을 알아보기 위하여 1차(15-Oct)에서 27차(18-Apr)에 이르는 월동기 전체의 상관관계(1~27상관)를 비롯하여 최저기온을 기록한 16차를 기준으로 1차(15-Oct)에서 16차(27-Jan)의 월동 전반기의 상관관계(1~16상관), 16차(27-Jan)에서 27차(18-Apr)에 이르는 월동 후반기의 상관관계(16~27상관)를 알아보았다. 대부분 월동 수조류는 기온에 대해 음의 상관성을 보였으나 쇠백로(Egretta garzetta), 민물가마우지(Phalacrocorax carbo) 및 왜가리(Ardea cinerea)는 양의 상관성을 보였다. 흰뺨검둥오리(Anas poecilorhyncha)가 기온의 변화에 대한 가장 높은 상관성(1-16상관, 7D/H3A, (r=-0.960, P<0.000000001)을 보였으며, 전체 월동 수조류는 모든 상관분석(1~27상관(r=-0.942, P<0.01), 1~16상관(r=-0.947, P<0.01), 16~27상관(r=-0.958, P<0.01))에서 4D/LA에 가장 높은 상관성을 보였다. 각각의 수조류와 기온에 대한 상관성은 종마다 기온(AT, HT, LT)과 기간(1D~7D)의 조건에 대해 각각 다르게 나타났다. 대부분의 종이 최고기온보다는 최저기온과의 상관성이 큰 것으로 나타났으며, 기온이 하강하는 월동 전반기에는 4일~7일의 기온에, 월동 후반기에는 1일~3일의 기온에 높은 상관성을 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6429-6433
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• 2012

To explore a possible new treatment for human ovarian cancer, we studied the effects of sodium valproate on the growth of the HO8910 human cell line. HO8910 cells were cultured in vitro and treated with different concentrations of sodium valproate. Cell proliferation, cell cycling, and apoptosis were measured by flow cytometry, cell morphology under a microscope, and expression levels of WWOX and P27 by Western blotting and RT-PCR. Tumor xenografts were established to determine in vivo effects of sodium valproate. Our results showed that cell proliferation was decreased with increasing concentration of sodium valproate, with features of cytoplasmic retraction and floating cells. Moreover, cell cycle analysis revealed a higher apoptosis rate and $G_0/G_1$ phase in the sodium valproate experimental group than in the control group. In addition, protein expression levels of WWOX and P27 were elevated. Importantly, sodium valproate decreased in vivo xenograft tumor burden and up-regulated WWOX and P27 expression in nude mice. In conclusion, sodium valproate might play a role in inhibition and control of ovarian cancer cell line HO8910 by inhibiting cell proliferation, interfering with the cell cycle and promoting apoptosis, so that it may be effective in the clinical treatment of ovarian cancer.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.96-101
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• 1991

Phenotypic changes after plasmid curing experiment suggested that the bacteriocin production phenotype ($Bac^{+}$) might be linked to a chromosomal DNA and the mucin production phenotype ($Muc^{+}$) might be linked to a 62.5 kilobase (kb) plasmid (pMUC62) in Lactobacillus plantarum 27 isolated from meat starter culture. The non-mucoid ($Muc^{-}$) variants were missing pMUC62 but they produced bacteriocin as the wild strain ($Bac^{+}$). There was no difference in antibiotic resistance and sugar fermentation patterns between the wild strain ($Bac^{+}$ $Muc^{+}$) and the nonmucoid ($Bac^{+}$ $Muc^{-}$) variants. Antimicrobial spectrum of bacteriocin produced by both wild strain and $Muc^{-}$ variant of Lb. plantarum 27 included strains of Pediococcus acidilactici (A, M, H), Pediococcus sp. isolated from meat, Lactobacillus sp. isolated from meat, Lb. plantarum NCDO 955 and Staphylococcus aureus 485. Neither of the tested Gram negative bacteria were inhibited by bacteriocin. Antimicrobial activity of crude bacteriocin was retained after autoclaving, DNase or catalase treatment and exposure from pHs 4 to 9 but was lost after treating with several proteolytic enzymes and exposure at pH 10.

• Nonlinear Functional Analysis and Applications
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• 제27권2호
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• pp.271-287
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• 2022

In this article, we shall prove a common fixed point theorem for two weakly compatible self-maps 𝒫 and 𝔔 on a dislocated metric space (M, d^*) satisfying the following ξ-weakly expansive condition: d^*(𝒫c, 𝒫d) ≥ d^* (𝔔c, 𝔔d) + ξ(∧(𝔔c, 𝔔d)), ∀ c, d ∈ M, where $${\wedge}(Qc,\;Qd)=max\{d^*(Qc,\;Qd),\;d^*(Qc,\;\mathcal{P}c),\;d^*(Qd,\;\mathcal{P}d),\;\frac{d^*(Qc,\;\mathcal{P}c){\cdot}d^*(Qd,\;\mathcal{P}d)}{1+d^*(Qc,\;Qd)},\;\frac{d^*(Qc,\;\mathcal{P}c){\cdot}d^*(Qd,\;\mathcal{P}d)}{1+d^*(\mathcal{P}c,\;\mathcal{P}d)}\}$$. Also, we have proved common fixed point theorems for the above mentioned weakly compatible self-maps along with E.A. property and (CLR) property. An illustrative example is also provided to support our results.

• 가정간호학회지
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• 제27권1호
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• pp.92-101
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• 2020

Purpose: This study aimed to identify the influence of social pressure related to appearance and body image dissatisfaction on mental health in adolescents. Methods: Participants included 293 high school students. The data were collected from March 4 to 29, 2019, and were analyzed using t-test, ANOVA, Pearson's correlation coefficient, and multiple linear regression with SPSS ver. 21. Results: Mental health was positively correlated with social pressure related to appearance and body image dissatisfaction. Factors influencing mental health were peer pressure (β=.28, p<.001) and parental pressure (β=.22, p<.001) related to appearance; body image dissatisfaction (β =.11, p=.034); "high" (β=-.18, p=.004) and "moderate" (β=-.22, p<.001) perceived school achievement; and "satisfied" response to school satisfaction (β=-.27, p=.028). These factors explained 32% of the variance in mental health. Conclusion: These results imply the need to develop effective intervention programs that enhance body image satisfaction to prevent poor mental health in adolescents.