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http://dx.doi.org/10.3746/jkfn.2002.31.2.329

Effect of Sarcodon aspratus Extract on Expression of Cell Cycle-Associated Proteins in HepG2 Cells  

배준태 (영남대학교 식품영양학과)
장종선 (영남대학교 식품영양학과)
이갑랑 (영남대학교 식품영양학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.2, 2002 , pp. 329-332 More about this Journal
Abstract
We investigated the effect of Sarcodon aspratus extract on expression of cell cycle regulators. Methanol extract of Sarcodon aspratus showed a growth suppression on HepG2. As shown by western blot analysis, the expressions of cyclin A and Dl known as cell cycle regulators were decreased after treatment of Sarcodon aspratus extract. On the other hand, the expression of cyclin Bl was increased in the presence of Sarcodon aspratus extract. Furthermore, the expression of p53, a tumor supressor gene, and p27, a cell cycle dependent protein kinase inhibitor, were increased, whereas the expression of PCNA was decreased. In conclusion, our study suggests that growth inhibitory effect of Sardodon aspratus methanol extract on HepG2 is induced by cell cycle arrest in the Gl phase caused by decrease in cyclin A, Dl expressions and increases in p53, p27 expression.
Keywords
Sarcodon aspratus; cell growth; cell cycle-associated proteins;
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