• KSBB Journal
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• v.28 no.1
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• pp.54-59
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• 2013

Three isolates, E. faecium P1, P2 and P3, from intestinal drugs of three phamaceutical companies, four clinical vancomycin resistant isolates, E. faecium V1, V2, V3 and E. faecalis V4, and three isolates, E. faecalis DW01, DW07 and DW14, from infant feces were tested for the presence of virulence genes, ace, agg, esp, efaA, gelE, sprE, vanA and vanB as well as fsrABC, regulatory genes of gelE and sprE, cylMBA, cytolysin activation genes and cpd, cob and ccf, pheromone genes by PCR and for their phenotype activities such as protease, biofilm formation, cell clumping and hemolysis. The genes encoding cell surface adherence proteins, ace, agg, esp and efaA, were predominantly amplified from the vancomycin resistant strain V4 and the fecal isolates DW01, DW07 and DW14. Both protease and biofilm formation activity were detected only from E. faecalis V4 from which the PCR products of gelE and spreE as well as fsrABC were amplified. The pheromone genes were amplified from the V4, DW01, DW07 and DW14 strains and these strains showed clumping activity. Biofilm formation was observed from the strains DW01, DW07 and DW14, all of which produced PCR products of pheromone, and V4 as well. Whole cytolysin regulator genes were amplified from DW01, DW07 and DW14 and ${\beta}$-hemolysis activity was detected from these strains. Any virulence genes or activities except the pheomone gene ccf were not detected from the pharmaceutical isolates, E. faecium P1, P2 and P3.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.2
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• pp.768-777
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• 2013

The purpose of this study is to be conducive to work improvement through analyzing turnover intention and organizational commitment of EMT within medical institution. The subjects of this study were randomly extracted 188 EMT. The survey period was conducted from May 3, 2011 to May 19 (May 3rd-4th in G Province, May 14th in B Metropolitan City, May 18th-19th in D Metropolitan City). An analytical method was made with SPSS WIN program(Version 12.0). As a result of research, the turnover intention was high in women(p<.001), in under 30 years old(about 4.9 times, p<.001), in the unmarried(about 4.3 times, p<.001), and in junior college graduate(about 4 times, p<.001). Also, the turnover intention was high in income with under 1,500,000 won(about 3.3 times, p<.001), in working career with under 5 years(about 3.2 times, p<.001), in feeling the legal work limit(about 1.8 times, p<.05), and in group with low job satisfaction(p<.001). In correlation between organizational commitment and job variable, the variables in age, marriage, academic background, turnover intention, income, career, and legal work limit except gender and job satisfaction showed positive correlation with organizational commitment(p<.01). As for the factors affected on the organizational commitment, the turnover intention(p<.01), the legal work limit(p<.01), and the job satisfaction(p<.001) had significant effect on organizational commitment.

• Electrical & Electronic Materials
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• v.7 no.4
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• pp.312-316
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• 1994

In this study, ln$_{1-x}$ Ga$_{x}$P alloy crystal which has different compositions were grown by the temperature gradient solution(TGS) method, and the properties of deep levels were measured in the temperature range of 9OK-450K. We find the four deep levels of E$_{1}$, E$_{2}$(248meV), E$_{3}$(386meV) and E$_{4}$(618meV) in GaP, which has composition of Ga in In$_{1-x}$ Ga$_{x}$P is one, and the trap densities of E$_{3}$ and E4 levels were 7.5*10$^{14}$ cm$^{-3}$ and 9*10$^{14}$ cm$^{-3}$ , respectively. A broad deep level spectra was revealed in In$_{1-x}$ Ga$_{x}$P whose composition of Ga, x, were 0.56 and 0.83, and the activation energy and trap densities were about 430meV and 6*10$^{14}$ cm$^{-3}$ , respectively.ectively.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.6
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• pp.365-369
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• 2010

In this study, we examined whether melatonin promotes apoptotic cell death via p53 in prostate LNCaP cells. Melatonin treatment significantly curtailed the growth of LNCaP cells in a dose- and time-dependent manner. Melatonin treatment (0 to 3 mM) induced the fragmentation of poly(ADP-ribose) polymerase (PARP) and activation of caspase-3, caspase-8, and caspase-9. Moreover, melatonin markedly activated Bax expression and decreased Bcl-2 expression in dose increments. To investigate p53 and p21 expression, LNCaP cells were treated with 0 to 3 mM melatonin. Melatonin increased the expressions of p53, p21, and p27. Treatment with mitogen-activated protein kinase (MAPK) inhibitors, PD98059 (ERK inhibitor), SP600125 (JNK inhibitor) and SB202190 (p38 inhibitor), confirmed that the melatonin-induced apoptosis was p21-dependent, but ERK-independent. With the co-treatment of PD98059 and melatonin, the expression of p-p53, p21, and MDM2 did not decrease. These effects were opposite to the expression of p-p53, p21, and MDM2 observed with SP600125 and SB202190 treatments. Together, these results suggest that p53-dependent induction of JNK/p38 MAPK directly participates in apoptosis induced by melatonin.

• Journal of Gastric Cancer
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• v.6 no.4
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• pp.227-236
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• 2006

Purpose: Methylation of gene regulatory elements plays an important role in gene inactivation without genetic alteration. Gastric cancer is one of the tumors that exhibit a high frequency of CpG island hypermethylation. The purpose of this study was to investigate the occurrence of CpG island hypermethylation in gastric carcinoma in relation to H. pylori infection, CIMP and clincopathologic variables. Materials and Methods: We investigated the promoter methylation Status of six genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin) and CIMP in 36 gastric carcinoma tissues as well as in nontumor tissues. CIMP status was investigated by examining the methylation status of MINT 1, 2, 12, 25 and 31. The methylation status of the promoter was examined by methylation-specific PCR (MSP) and H. pylori infection was examined by histological diagnosis after staining with Warthin-Starry silver. Results: Among the 36 gastric carcinoma tissues, DNA hypermethylation was detected in the following frequencies: 14 (38.9%) for p14, 13 (36.1%) for p16, 8 (22.2%) for MGMT, 10 (27.8%) for COX-2, 21 (58.3%) for E-cadherin, and 6 (16.7%) for hMLH1. The frequencies for MINT1 and MINT25 hypermethylation were significantly higher in tumor tissues than in nontumor tissues. 16 (44.4%) of the 36 gastric carcinoma tissues were positive for the CIMP CIMP-H tumors were associated with older patients and larger tumor size than CIMP-L tumors. We found a significant association between the presence of the CIMP and hypermethylation of p16. Hypermethylation of p16 and MINT2 were significantly different when compared by age. MINT1 gene methylation was significantly associated with H. pylori infection (P=0.004). Conclusion: Our results suggest that aberrant hypermethylation of multiple tumor related genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT1, 2, 12, 25, 31) occurs frequently in gastric carcinoma tissues. The hypermethylation of MINT1 was significantly higher in the tumor tissues and was associated with H. pylori infection.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.41 no.1
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• pp.9-14
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• 2004

In this paper, temperature dependence of effective ionization coefficient in Si is formulated as a single polynomial function of temperature, which allows analytical expressions for breakdown voltage of Si $p^+n$ junction as a function of temperature. The analytical breakdown voltages agree well with the simulation as well as the experimental ones reported within $3\%$ in error for the doping concentrations in the range of $10^{14}cm^{-3}{\~} 10^{17}cm^{-3}$ at 100K, 300K and 500K.

• Journal of Convergence for Information Technology
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• v.9 no.5
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• pp.190-200
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• 2019

This study aimed to investigate the associations between subjective oral health status and OHIP-14 among Chinese residents. A survey was conducted on the Chinese residing in Busan. As a result of analyses on the collected data with SPSS 25.0 the OHIP-14 was significantly different depending on the subjective oral health status including mastication discomfort experience, toothache experience, oral temperature sensitive response experience, gingival bleeding and disease experience, dry mouth experience and halitosis experience. The major variables were all positively correlated. Among the factors of subjective oral health status affecting the oral health-related quality of life, the influences of mastication (p<0.001) and halitosis (p<0.05) were significant. There is a need for a medical support system that can provide practically oral health policies and support Korean language for foreigners.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.06a
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• pp.225-225
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• 2004

원전 1차 냉각계통내 화학첨가제인 amine 또는 과산화수소를 사용하면서 $^{14}N$(n, P)$^{14}C$와 $^{17}O$(n, $\alpha$)$^{14}C$의 핵반응으로 생성된 $^{14}C$는 냉각수내에서 방사성 폐기물로 존재하게 된다. 이들 방사성 폐기물은 pH에 따라 다르지만 수용액상에서는 대부분 $CO_2$, $H_2CO_3$, ${HCO_3}^-$ 및 ${CO_3}^{2-}$로 존재하고, 나머지 약 20% 정도는 유기성 탄소로는 메탄이 존재하는 것으로 알려져 있다. 폐이온 교환수지 내에 존재하는 $^{14}C$는 시간이 경과함에 따라서 방향족 화합물로 이온교환이 발생할 수가 있다.(중략)

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.4
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• pp.567-578
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• 2018

In this study, to investigate the effect of the stress reduction of group piles by dynamic loading, a dynamic p-y curve was established and the dynamic p-multiplier was calculated. Dynamic numerical analysis was performed by input sinusoidal waves to the bottom of the pile - ground system for $2{\times}2$ group pile, single pile and $5{\times}5$ group pile, single pile in dry sandy soil, and the pile spacing was changed to 2.5 and 5.0 times of the pile diameter. By establishing and comparing the dynamic p-y curves of the single pile and group piles, the dynamic group pile effect of the piles according to the pile center spacing and row position of the group pile piles is analyzed. $5{\times}5$ showed symmetry of the dynamic P-multiplier value around the pile origin coordinate. The dynamic p-multiplier value at the single pile, $5{\times}5$ pile (pile spacing: 2.5D) is 0.26 ~ 0.30 at the pile number 3, pile number 23, 0.14 pile number 13, and 0.14 ~ 0.38 at the pile number 5, pile number 18. These values differed from the static p-multiplier, especially due to the different loading conditions. The dynamic p-multiplier ($P_{dm}$) estimation through various types of input dynamic loads is expected to be used for dynamic design and analysis of group pile-ground systems of civil foundation structures.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.295-302
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• 2019

This study was conducted to investigate the effects of adolescent health behavior on obesity using an online health behavior survey. The subjects of this study were 12,090 middle school students and 14,248 high school students among 26,338 Korean youths that responded to an online survey of youth health behaviors in 2018 (14th). There were significant differences in lifestyle, mental factors, exercise habits, and sitting habits as health behavior factors. The risk of obesity was higher in smokers (p<0.001), drinkers (p<0.001), those who ate breakfast less than four times a week (p<0.001), those who consumed fruit less than four times a week (p<0.001) and those who ate fast food less than two times a week (p<0.001). The obesity rate was higher in people with high-stress (p<0.05). Exercise habit as a factor was higher in the obesity rate than in physical activity by three times as much (p<0.001), whereas students categorized as muscular (p<0.01) had one to two times more physical activity (p<0.001). Students who had less than six hours of learning purpose were found to have higher obesity rates than those with more than six hours of learning purpose (p<0.001). In conclusion, the obesity rate did not increase with prolonged sitting habits, but did increase with longer sitting time except for those who studied purpose folly. Therefore, it is necessary to have a set time for internet use, as well as to educate schools about proper lifestyle, and to promote healthy exercise habits.