• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.5
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• pp.697-704
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• 2022

This study was investigated to evaluate the level of acute TAN (total ammonia nitrogen) and NO₂^--N concentrations at pH levels of 6.0, 7.0 and 8.0 for 96 h in juvenile marbled eel Anguilla marmorata (total length 209.0±22.02 mm and body weight 13.0±5.01 g). The result of the present study showed that the survival rate of juvenile eel at TAN concentrations 0, 100, 200, 300, 400, and 500 ppm at pH 6.0, pH 7.0, and pH 8.0 were 100, 100, 96.7, 74.4, 31.1, and 0%; 100, 82.2, 61.1, 36.7, 0, and 0%; and 98.9, 55.6, 8.9, 0, 0, and 0%, respectively. In addition, the survival rate of juvenile eel at NO₂^--N concentrations 0, 100, 200, 300, 400, and 500 ppm at pH 6.0, pH 7.0, and pH 8.0 were 100, 43.3, 21.7, 0, 0, and 0%; 100, 76.7, 65.0, 43.3, 21.7, and 13.3%; and 100, 100, 100, 88.3, 78.3; and 58.3% respectively. The 96h-LC₅₀ at pH 6.0, 7.0, and 8.0 were 332, 235, and 167 mg/L for TAN, and 188, 296, and 711 mg/L for NO₂^--N, respectively. The acute toxicity of TAN to juvenile eel increased exponentially with increase in pH, whereas the acute toxicity of NO₂^--N to juvenile ell increased with low levels of pH and lengthening of exposure time to NO₂^--N.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.10
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• pp.1440-1444
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• 2000

Two feeding trials were conducted to evaluate the effects of inclusion levels of dietary vitamin and trace mineral (VTM) premixes on growth and nutrient digestibility in growing pigs. A total of 112 pigs ($24.82{\pm}3.22kg$) were employed for 49 days (exp. 1), and 168 pigs ($21.64{\pm}1.41kg$) for 40 days (exp. 2) in completely randomized block designs. Treatments were: 1) 100%, 2) 150%, 3) 200% and 4) 250% NRC (1998) requirement of VTM in exp. 1, and the ratio of vitamins to trace minerals at 1) 100:100%, 2) 100:150%, 3) 150:100% and 4) 150:150% of NRC (1998) requirement in exp. 2. Basal diets for feeding trials were formulated to contain 3,310 kcal ME/kg and 18% crude protein, and contained 0.25% chromic oxide as an indigestible marker for digestibility trials. Increasing dietary VTM premix in growing pigs had linear and quadratic effects (p<0.05) on ADG, and feed conversion ratio was also improved (p<0.05) as VTM premix was increased by 150-250% of NRC (1998) requirements in exp. 1. Adding vitamin to trace mineral premixes at 150% NRC (1998) over the control improved (p<0.05) ADG and feed efficiency in growing pigs, but performances were not improved by vitamin nor by trace mineral premixes alone (p>0.15) (exp. 2). There were no differences (p>0.05) in the digestibilities of energy, crude protein and fat among dietary treatments. However, increasing dietary VTM premix in growing pigs had a linear effect (p<0.05) on the digestibilities of calcium and phosphorus. The 200 or 250% fed group showed improved (p<0.05) calcium digestibility, and 250% fed group also showed improved (p<0.05) phosphorus digestibility as compared to 100% or 150% fed group (exp. 1). The digestibilities of Ca and P were higher (p<0.05) in 150% addition of vitamins than in 150% addition of trace minerals in the diet (exp. 2).

• Environmental Analysis Health and Toxicology
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• v.6 no.1_2
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• pp.39-57
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• 1991

The effect of red ginseng ethanol extract on the immunotoxicity of diethylstilbestrol (DES) was studied in ICR mice. ICR male mice were divided into S groups (10 mice/group), and red ginseng ethanol extract (50, 100 and 200 mg/kg body wt., respectively) and DES (1 mg/kg body wt.) were injected intraperitoneally (i.p.) to ICR mice once a day for 2 weeks. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune response were evaluated by humoral immunity, cell-mediated immunity, non-specific immunity, and circulating leukocyte counts. The results of this study were summarized as followings: 1. The DES-treated control group as compared with normal group showed the tendency to decrease body weight rate and relative liver weight, decreased both humoral and cellular immune responses, phagocyte activity, and circulating leukocyte counts, but increased the natural killer (NK) cell activity. 2. Compared with the DES-treated control group, DES plus red ginseng ethanol extract-treated groups significantly decreased the body weight rate (P<0.01). Relative liver weight was significantly decreased in DES plus red ginseng ethanol extract (50mg/kg)-treated group (P<0.01), but significantly increased in DES plus red ginseng ethanol extract (100mg/kg)-treated group (P<0.01). Relative spleen and thymus weights were significantly enhanced in DES plus red ginseng ethanol extract (100 mg/kg)-treated group (P<0.01), but significantly decreased in DES plus red ginseng ethanol extract (200 mg/kg)-treated group (P<0.01). 3. Both humoral and cellular immune responses were significantly decreased in DES plus red ginseng ethanol extract-treated groups rather than in the DES-treated control group (P<0.01). Especially, it weakened the decrease in DES plus red ginseng ethanol extract (100 mg/kg)-treated group. 4. Phagocyte activity and circulating leukocyte counts were significantly decreased in DES plus red ginseng ethanol extract-treated groups rather than in the DES-treated control group (P<0.01). Especially, it weakened the decrease in DES plus red ginseng ethanol extract (100 mg/kg)-treated group. NK cell activity was significantly enhanced in DES plus red ginseng ethanol extract (100 mg/kg)-treated group (P<0.01), but significantly decreased in DES plus red ginseng ethanol extract (50 and 200 mg/kg)-treated groups (P<0.01).

• Journal of Environmental Science International
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• v.5 no.5
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• pp.611-617
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• 1996

The effects of nutritional sources on growth of Pseudomonas sp. P2 were investigated in medium containing biphentyl as a carbon source. To determine characterization of Pseudomonas sp. P2, the incubation time was determined to 100 h of the log phase in the growth curve. The optimal compositions for the growth of Pseudomonas sp. P2 degrading polychlorinated biphenyls (PCBs) were 1000 mg/L $NH_4NO_3$, 1000mg/L KH_2PO_4$, 100mg/L MgSO_4$.$7H_2O$, 30mg/L $CaCl_2$.$2H_2O$, 200mg/L NaCl, and 10mg/L $FeSO_4$.$7H_2O$. Pseudomonas sp. P2 showed the degradability of 59.3%, 57.6%, 51.4%, and 48.7% at 500mg/L, 1000mg/L, 1500mg/L, and 2000mg/L of the PCBs within insulating oil after 100 h incubation under the optimum conditions, respectively.

• Food Science of Animal Resources
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• v.28 no.1
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• pp.9-13
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• 2008

The objective of this study was to evaluate the effect of different ratios of horse meat and pork (horse meat: pork=0:100, 50:50, 100:0) on the quality and sensory characteristics of press hams. Press ham containing 100% horse meat had a higher moisture content (%) and lower fat content (%) than the other hams (p<0.05). The redness (hunter a value) and yellowness (hunter b value) were significantly higher in press ham containing 100% horse meat, whereas the lightness (hunter L value) was significantly lower than other hams (p<0.05). With regard to textural properties, press ham containing 100% horse meat had significantly higher springiness, but there were no significant differences among the ham products in regard to hardness, cohesiveness, gumminess and chewiness (p>0.05). The results of sensory tests showed that press ham containing 100% horse meat had significantly lower color and acceptability scores compared to other hams (p<0.05). With regard to mineral contents, Na, Fe, Mn, and Zn were significantly higher in press ham containing 100% horse meat (p<0.05).

• Development and Reproduction
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• v.15 no.3
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• pp.223-230
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• 2011

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants derived from incomplete combustion of carbons and crude oil. In this study, we investigated the effects of benzo[a]pyrene (B[a]P), a representative PAHs on in vitro sex steroid hormone production and germinal vesicle breakdown (GVBD) using isolated oocytes of longchin goby (Chasmichthys dolichognathus) and chameleon goby (Tridentiger trigonocephalus). Oocytes in diameters of 0.8-0.9 (end vitellogenic stage) and 0.9-1.0 mm (germinal vesicle migratory stage) from longchin goby and 0.5 mm (fully vitellogenic stage) from chameleon goby were used. In GVBD assay, B[a]P at 10 nM stimulated GVBD in the oocytes of 0.8-0.9 mm from longchin goby. B[a]P at 1 nM stimulated GVBD in the oocytes with diameter 0.5 mm from chameleon goby. In steroid production from oocytes of longchin goby, B[a]P at 100 nM decreased testosterone (T) production, B[a]P at 1,000 nM increased estraiol-17 (J (E2) production and 10 and 100 nM increased $17,20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) production in the oocytes with diameter 0.8-0.9 mm. B[a]P at 1,000 nM increased E2 production, 100 and 1,000 nM increased $17{\alpha}20{\beta}P$ production in the oocytes with diameter 0.9-1.0 mm. In steroid production of oocytes from chameleon goby, B[a]P at 1,000 nM increased $E_2$ production. B[a]P at 10 nM increased $17{\alpha}20{\beta}P$ production. In the ratio of $E_2$ to T ($E_2$/T), B[a]P at 100 and 1,000 nM increased $E_2$/T in the oocytes of longchin goby. B[a]P at 100 nM also increased $E_2$/T in the oocytes of chameleon goby. Taken together, these results suggest that B[a]P have not only weak estrogenic effects but progestogenic effects on oocyte maturation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.73-73
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• 2018

최근 매실나무를 기주로 한 겨우살이의 인공재배에 성공함에 따라, 식품 소재로 이용하기 위해 일반성분을 분석하였다. 경기도 남양주시에서 매실나무를 기주로 한 인공재배 겨우살이를 채취하여 동결건조한 후 수분, 조단백질, 조지방, 회분, 탄수화물, 베타카로틴, 비타민 B1, 비타민 B2, 비타민 B3, 비타민C, Ca, Fe, K, Na 그리고 P의 함량을 분석하였다. 조단백질, 조지방, 회분, 베타카로틴, Ca, Fe, K, Na 그리고 P은 AOAC 방법을 이용하여 분석하였고, 탄수화물은 탄수화물(%) = 100 - (조단백질% + 조지방% + 회분% + 수분%) 계산식을 활용하여 산출하였다. 수분, 비타민 B1, 비타민 B2, 비타민 B3, 비타민 C는 식품공전에 제시된 방법에 따라 분석하였다. 동결건조한 매실나무겨우살이의 일반성분은 탄수화물 58.90%, 단백질 22.36%, 회분 11.11%, 지방 5.37%, 수분 2.26% 순으로 함유하였다. 비타민의 함량은 비타민 C 4.78mg/100g, 나이아신 1.87mg/100g, 비타민 A 1.42mg/100g, 비타민 B2 0.73mg/100g 그리고 비타민 B1 0.30 mg/100g의 순서로 많이 함유하였다. 무기질은 K 3141.54mg/100g, Ca 977.77mg/100g, P 309.91 mg/100g, Na 20.13mg/100g 그리고 Fe 8,24mg/100g의 순서로 많이 함유하였다.

• Journal of dental hygiene science
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• v.7 no.1
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• pp.13-20
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• 2007

This study is aimed at providing basic data for measures to prevent dental hygienists who are highly exposed to hepatitis-B virus due to their characteristic working conditions from being infected of it. In order to determine their perception and the extent of their exposure to the risk of infection with hepatitis-B virus, a survey was conducted with questionnaires distributed to 354 dental hygienists who are working at dental offices in Seoul. Gyeongi and Jeolla provincial areas. From the survey, following conclusions could be drawn: 1. Sixty three point seven to 100 percent of them are found to have been vaccinated against hepatitis-B virus as is generally expected from their working environment. 2. Their general characters are found to be statistically significant in terms of vaccinating point of time among those who have been vaccinated. Most respondents are either vaccinated one year or 5 years ago. The completion of 3 requested vaccinations lies in the level of 64.3%~100% depending on the general characters. By age, the best result comes from the age group of 25~29, followed by the groups older than 30 and 20-24 in order (p < 0.05). Formation of antibody belongs to the level of 45%~100% generally. But statistically significant of them is the extremely unhealthy state with 100%, followed by normal, generally healthy, very healthy and not healthy in sequence (p < 0.05). 3. In terms of family's clinical history, it is known that the older (p < 0.01), the more unhealthy (p < 0.01) and the married (p < 0.01), the more possibility of having anamnesis of hepatitis-B virus infection. 4. The level of knowledge about Hepatitis-B virus is shown to be in the extent of 4.39~5.01 out of maximum 8 points in general terms. 5. It is revealed that there are high chances for dental hygienists to get spattered with body matters of patients like blood or sputum on their faces in general characters (87.%~100%). 6. A high rate (75%~100%) of respondents has experienced being shot by needles. The older(p < 0.05) and the longer(p < 0.05) their career, the higher the rate becomes.

• Journal of the Korean Magnetic Resonance Society
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• v.20 no.3
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• pp.95-101
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• 2016

Apolipoprotein B-100 (Apo-B100) is a major component of low density lipoprotein (LDL). Apo B-100 protein has 4,536 amino acid sequence and these amino acids are classified into peptide groups A to G with subsequent 20 amino acids (P1-P302). The peptide groups were act as immunoglobulin (Ig) antigens which oxidized via malondialdehyde (MDA). The mimetic peptide P1 (EEEMLENVSLVCPKDAT RFK) out of D-group peptides carrying the highest value of IgG antigens were selected for structural studies that may provide antigen specificity. Circular Dichroism (CD) spectra were measured for peptide secondary structure in the range of 190-250 nm. Experimental results show that P1 exhibit partial of ${\beta}-sheet$ and random coil structure. Homonuclear (COSY, TOCSY, NOESY) 2D-NMR experiments were carried out for NMR signal assignments and structure determination for P1. On the basis of these completely assigned NMR spectra and distance data, distance geometry (DG) and Molecular dynamics (MD) were carried out to determine the structures of P1. The proposed structure was selected by comparisons between experimental NOE spectra and back calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P1 obtained upon superposition of all atoms was in the range $0.33{\AA}$. The solution state P1 has mixed structure of ${\beta}-sheet$ (Glu[1] to Cys[12]) and random coil (Pro[13] to Lys[20]). These NMR results are well consistent with secondary structure from experimental results of circular dichroism. Structural studies based on NMR may contribute to the studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

• Journal of Plant Biology
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• v.26 no.1
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• pp.17-32
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• 1983

The aboveground production, nutrient distribution and nutrient cyclings were compared between two Phragmites communis communities growing in the different salt contents of soil in a coastal salt marsh. Inorganic nutrient contents of soil for plant growth were greater at the low salt stand than at the high salt stand except for sodium(Na). Maximum aboveground biomass of the plant at the low and the high salt stands were 2,533 and 1,719 g dw/$m^2$, respectively, in August. Seasonal changes of nutrient content of biomass in dry weight decreased with growth except for Na. Nutrient contents in biomass per unit land area increased continuously as biomass increases, although the amount of potassium(K) reached the maximum content in July and thereafter decreased. Vertical distributions of total nitrogen(T-N) and phosphorus(P) increased with plant height, but Na showed the reverse trend. That of K was similar to the patterns for T-N and P in the leaves, and to the pattern of Na in the stems. The Na was greatly accumulated in underground biomass but transported scarcely to aboveground. At the low and the high salt stands, the ratios of the inorganic nutrients contained in the plant were 100 : 66 for T-N, 100 : 61 for P, 100 : 62 for K and 100 : 97 for Na. the ratios of the amounts of nutrients retrieved to soil were 100 : 242 for T-N, 100 : 408 for P, 100 : 127 for K and 100 : 269 for Na, respectively. Turnover times of the T-N, P, K and Na in the communities were 56, 1, 15 and 174 years at the low salt stand, and 75, 2, 24 and 323 years at the high salt stand, respectively. In nutrient cyclings, all of the nutrients retrieving to soil were less than uptake by plant. Among the nutrient, especially P is expected to be exhausted from soil, sooner or later, because of the harvest by men.