• 한국강구조학회 논문집
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• 제17권3호통권76호
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• pp.347-356
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• 2005

최근 들어 강구조 공장건축은 상당부분 P.E.B.시스템으로 지어지고 있으나 관련기술이 대부분 외국에서 수입된 것으로 전용프로그램(예, MBS, LTI 등)에만 의존하고 있고 국내의 설계지침이 없어서 AISC-ASD에 의해 설계하고 있다. 또한 P.E.B.골조의 구조적 거동을 검토한 연구 및 H형강을 이용한 요소기술 개발이 부족한 실정에 있다 특히, 기둥-보(rafter) 접합부는 Extended type end plate에 의한 접합에만 의존하여 과다설계의 경향이 있으므로. 접합부에 대한 구조적 검토가 필요하다. 따라서 이 연구에서는 P.E.B.시스템 공장건물에서 H형강-보(rafter)의 접합부(Extended/Flush type)의 구조성능을 실험적으로 평가하여 구조적, 경제적으로 우수한 P.E.B. 시스템 골조의 설계를 위한 기초자료를 제공하고자 한다.

• 한국강구조학회 논문집
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• 제19권5호
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• pp.549-558
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• 2007

이 연구는 P.E.B. 골조 시스템 공장 현장에서 가장 많이 사용하고 있는 훅 볼트 형상의 골조 가새의 사용현황을 조사하여 그 문제점을 파악하기 위하여, 다양한 형상의 가새 (예를 들면, 로드 바, 로드 슈, 앵글)에 대한 구조성능실험을 실시한다. 그 실험결과의 분석, 비교를 통해서, 훅 볼트 형 가새의 기술적 한계를 평가하고, P.E.B. 골조에 적합한 인장가새의 기술을 제안한다.

• Journal of Information Processing Systems
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• 제12권1호
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• pp.46-56
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• 2016

Dynamic textures are videos that exhibit a stationary property with respect to time (i.e., they have patterns that repeat themselves over a large number of frames). These patterns can easily be tracked by a linear dynamic system. In this paper, a model that identifies the underlying linear dynamic system using wavelet coefficients, rather than a raw sequence, is proposed. Content based threshold filtering based on Set Partitioning in a Hierarchical Tree (SPIHT) helps to get another representation of the same frames that only have low frequency components. The main idea of this paper is to apply SPIHT based threshold filtering on different bands of wavelet transform so as to have more significant information in fewer parameters for singular value decomposition (SVD). In this case, more flexibility is given for the component selection, as SVD is independently applied to the different bands of frames of a dynamic texture. To minimize the time complexity, the proposed model is implemented on a graphics processing unit (GPU). Test results show that the proposed dynamic system, along with a discrete wavelet and SPIHT, achieve a highly compact model with better visual quality, than the available LDS, Fourier descriptor model, and higher-order SVD (HOSVD).

• 정보보호학회논문지
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• 제21권3호
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• pp.183-188
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• 2011

모바일 응용에서 MPEG 비디오 스트림 등 대용량 데이터의 이용이 증가함에 따라, 전송되는 대용량 데이터의 정보 보호가 중요한 문제로 부각되고 있다. 대용량 데이터의 효과적인 보호를 위한 부분 암호화 방법으로 SECMPEG이 존재한다. 그러나 SECMPEG의 보안 레벨은 B- 나 P-프레임에 비해 상대적으로 용량이 큰 I-프레임을 모두 암호화 하기 때문에, 모바일 응용에 적용하기에는 암호화 오버헤드가 크다는 문제가 있다. 그러므로 MPEG2 표준의 압축 특성을 분석하여 I 프레임에서의 부분 암호화를 효과적으로 적용할 필요가 있다. 본 논문에서는 수신단에서 I-프레임 내의 에러 전파 특성을 이용하는, 슬라이스 레벨에서의 부분 암호화 방법을 제안한다. 실험 결과를 통하여 SECMPEG 보안 레벨 3과 비교하여 제안 방법은 보안 성능의 저하없이 수행 시간을 약 30배 이상 단축함을 확인하였다.

• Food Science and Biotechnology
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• 제18권2호
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• pp.396-401
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• 2009

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.

• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.211-218
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• 2023

A cryptic plasmid (pTH32) was characterized from Tetragenococcus halophilus 32, an isolate from jeotgal, Korean traditional fermented seafood. pTH32 is 3,198 bp in size with G+C content of 35.84%, and contains 4 open reading frames (ORFs). orf1 and orf2 are 456 bp and 273 bp in size, respectively, and their translation products showed 65.16% and 69.35% similarities with RepB family plasmid replication initiators, respectively, suggesting the rolling-circle replication (RCR) mode of pTH32. orf3 and orf4 encodes putative hypothetical protein of 186 and 76 amino acids, respectively. A novel Tetragenococcus-Escherichia coli shuttle vector, pMJ32E (7.3 kb, Em^r), was constructed by ligation of pTH32 with pBluescript II KS(+) and an erythromycin resistance gene (ErmC). pMJ32E successfully replicated in Enterococcus faecalis 29212 and T. halophilus 31 but not in other LAB species. A pepA gene, encoding aminopeptidase A (PepA) from T. halophilus CY54, was successfully expressed in T. halophilus 31 using pMJ32E. The transformant (TF) showed higher PepA activity (49.8 U/mg protein) than T. halophilus 31 cell (control). When T. halophilus 31 TF was subculturd in MRS broth without antibiotic at 48 h intervals, 53.8% of cells retained pMJ32E after 96 h, and only 2.4% of cells retained pMJ32E after 14 days, supporting the RCR mode of pTH32. pMJ32E could be useful for the genetic engineering of Tetragenococcus and Enterococcus species.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.593-598
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• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• Journal of Microbiology
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• 제40권1호
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• pp.43-50
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• 2002

A cluster of genes encoding the pyruvate dehydrogenase complex (PDC) of Streptomyces seoulensis, a Gram-positive bacterium, was cloned and sequenced. The genes of S. seoulensis consist of four open reading frames. The first gene, lpd, which encodes a lipoamide dehydrogenase, is followed by pdhB encoding a dihydrolipoamide acetyltransferase (E2p), pdhR, a regulatory gene, and pdhA encoding a pyruvate dehydrogenase component (Elp). Elp had an unusual homodimeric subunit, which has been known only in Gram-negative bacteria S. seoulensis E2p contains two lipoyl domains like those of humans and Streptomyces faecalis. The pdhR gene appears to be clustered with the structural genes of S. seoulensis PDC. The PdhR-overexpressed S. seoulensis howed growth retardation and the decrease of Elp, indicating that PdhR regulates the function of PDC by repressing the expression of Elp. A strain of Streptomyces licidans overexpressing S. seoulensis PdhR showed a significant decreasein the level of actinorhodin, implying a regulatory role for Streptomyces PDC in antibiotic biosynthesis.

• 미생물학회지
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• 제52권1호
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• pp.110-115
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• 2016

남극 해양세균 Pseudoalteromonas sp. PAMC 21150에서 분리한 소형 플라스미드(small plasmid, pDK4)의 크기는 3,480bp이고 G+C 함량은 41.64%이며, 3개의 open reading frames(ORFs)을 포함하고 있다. 3개의 ORF는 replication initiation protein (RepA), conjugative mobilization protein (Mob), 그리고 기능이 밝혀지지 않은 단백질을 코팅하고 있다. PCR 반응으로 증폭한 pDK4를 Escherichia coli high-copy pUC19 클로닝 벡터에 삽입하여 fusion vector (pDOC153)를 제작하였고, pDOC 153에 chloramphenicol 저항성 유전자를 삽입하여 ampicillin/chloramphenicol 저항성 Pseudoalteromonas - Escherichia coli 셔틀 벡터(shuttle vector; 7,216 bp 크기; pDOC155)를 제작하였다. 북극 해양세균 P. issachenkonii PAMC 22718이 보유한 2개의 유전자(TonB-dependent receptor gene, chi22718_IV, and exochitinase gene, chi22718_III)를 pDOC155에 삽입하여 두 개의 pDOC155 변형체(pDOC158, pDOC165)를 제작하였다. pDOC158 혹은 pDOC165을 이용하여 triparental mating 방법에 의해 플리스미드 미보유 해양세균인 Pseudoalteromonas sp. PAMC 22137를 형질전환하였다. PCR을 이용한 유전자 증폭실험을 통해서, pDOC158와 pDOC165에 삽입된 유전자들은 Pseudoalteromonas sp. PAMC 22137와 E. coli $DH5{\alpha}$ 내에서 안정적으로 유지되는 것을 확인하였다. 위의 결과는 셔틀 벡터 pDOC155는 Pseudoalteromonas spp. 유래 유전자들을 다른 Pseudoalteromonas spp. 세포 안으로 전달할 수 있는 새로운 유전자 전달시스템으로 이용될 수 있음을 보여주었다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.94.2-95
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• 2003

E. intermedium 60-2G possessing a strong ability to solubilize insoluble phosphate, has plant growth-promoting activity, induced systemic resistance activity against scab pathogen in cucumber, and antifungal activity against various phytopathogenic fungi. The phosphate solubilizing activity of 60-2G may be mainly accomplished by production of gluconic acid through a direct extracellular oxidation of glucose by glucose dehydrogenase that required a PQQ cofactor for its activation. A pqq gene cluster conferred Phosphate-solubilizing activity in E. coli DH5${\alpha}$ was cloned and sequenced. The 6,783 bP pqq sequence had six open reading frames (from A to F) and showed 50-95％ homology to pqq genes from other bacteria. The E. coli strain expressing the pqq genes solubilized phosphate from hydroxyapatite after a pH drop to 4.0, which paralleled in time the secretion of gluconic acid. To study the role of PQQ in biocontrol traits of E. intermedium, PQQ mutants of 60-2G were constructed by marker exchangee mutagenesis. The PQQ mutants of E. intermedium were lost activities of solubilizing phosphate, growth inhibition of phytopathogenic fungi, and plant growth promotion. These findings suggest that PQQ plays an important role, possibly activation of certain enzymes, in several beneficial bacterial traits of E. intermedium by as yet an unknown mechanism.