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Sequence Analysis and Functional Expression of the Structural and ]Regulatory Genes for Pyruvate Dehydrogenase

Hwan Youn (Laboratory of Biophysics, School of Biolgical Sciences, and Research Center for Molecular Microbiology, Seoul National University)
Jangyul Kwak (Department of Microbiology, Ohio State University)

Publication Information

Journal of Microbiology / v.40, no.1, 2002 , pp. 43-50 More about this Journal

Abstract

A cluster of genes encoding the pyruvate dehydrogenase complex (PDC) of Streptomyces seoulensis, a Gram-positive bacterium, was cloned and sequenced. The genes of S. seoulensis consist of four open reading frames. The first gene, lpd, which encodes a lipoamide dehydrogenase, is followed by pdhB encoding a dihydrolipoamide acetyltransferase (E2p), pdhR, a regulatory gene, and pdhA encoding a pyruvate dehydrogenase component (Elp). Elp had an unusual homodimeric subunit, which has been known only in Gram-negative bacteria S. seoulensis E2p contains two lipoyl domains like those of humans and Streptomyces faecalis. The pdhR gene appears to be clustered with the structural genes of S. seoulensis PDC. The PdhR-overexpressed S. seoulensis howed growth retardation and the decrease of Elp, indicating that PdhR regulates the function of PDC by repressing the expression of Elp. A strain of Streptomyces licidans overexpressing S. seoulensis PdhR showed a significant decreasein the level of actinorhodin, implying a regulatory role for Streptomyces PDC in antibiotic biosynthesis.

Keywords

pyruvate dehydrogenase; dihydrolipoamide acetyltransferase; lipoamide dehydrogenase; PdhR; actinorhodin.;

Citations & Related Records

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