• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1890-1893
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• 2007

A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.

• Journal of Korean Public Health Nursing
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• v.31 no.1
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• pp.58-70
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• 2017

Purpose: This study was conducted to determine if women's depression is influenced by their self esteem, stress, and social support. Methods: This study was a descriptive survey, and data were collected from the November 1 to December 20, 2015 using a self-reported questionnaire. The measurements were SES for self esteem, and CES-D for depression. A total of 165 middle-aged women participated through convenient sampling. Results: There were significant difference in self-esteem according to education level and monthly income. Additionally, there was an inverse correlation between stress and self esteem, as well as a significant strong correlation between self esteem, stress, social support and depression. Finally, multiple regression showed that self-esteem and stress had significant effects on depression, and their explanatory power was ${\Delta}R2(%)=47.9$ (${\Delta}F=66.526$, p<.001), p<.001). Conclusion: This study confirmed that depression was closely related to self-esteem and stress among middle-aged women. Based on these findings, we suggest that the health policies of reducing depression for middle-aged women should focus on reducing stress and reinforcing their self-esteem.

• Journal of the Korean Society of Food Culture
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• v.18 no.6
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• pp.515-526
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• 2003

The object of this study was to investigate the relationship between the eating habits of high(4th, 5th, 6th) grade elementary school students and parental behavior characteristics(affection, rational guidance, overprotect and neglect). The parenting behavior scale and demographic variables, eating habits and food frequency were administered to the subjects. In this investigation, the subjects were 396 boys and 337 girls in Chunju city in Korea, 50.1% of the mothers was in the age from 35 to 39 years and 57.8% of the mother had an occupation. Data were analyzed by using a SPSS PS package. Significant differences and correlation among variables were determined by the frequency, $x^2-test$ and pearson's correlation coefficient. Eating habits was significantly influenced by mother's employment status, but was not affected by the levels of mother's education and income. Parental behavior characteristics was significantly related to the children's snacks habits(p<0.05, p<0.01), but have no connection with dietary habits and the eating out. The survey revealed higher percentage of the children who had taken affectionate, reasonable and positively reinforced child care, answered they enjoy meals and the other groups answered they did not. Also frequency of having soup, green vegetables, fruits, dairy products had positive relationship with affective, reasonable rearing attitude, but negative relationship with neglective rearing attitude. And the survey showed children who were reared in overprotect had chocolate, candy and the like in large quantities.

• Archives of Pharmacal Research
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• v.21 no.5
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• pp.521-526
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• 1998

The physicochmical properties of recombinant hepatitis B surface antigen (r-HBsAg), which was expressed in C127 mammalian cell were studied. Using roller bottle culture in DMEM supplemented with fetal bovine serum, 10-15 mg/L of r-HBsAg was produced with about 31% of purification yield. The purity of r-HBsAg by HPLC was 99.8% and electron microscopic examination showed homogeneous spherical particle with 22 nm in diameter, a morphological characteristic of HBsAg. The density of r-HBsAg by CsCI density gradient method was 1.19g/ml and the isoelectric point by Mono $P^{TM}$ HR 5/20 column was 4.6. The analysis of subunit protein pattern using SDS-PAGE followed by scanning densitometry gave 81.3% of S protein and 18.7% of pre-S protein. fluorophore-assisted-carbohydrate-electrophoresis analysis showed the relative amount of carbohydrate to protein was 1.7% and it smajr component was N-acetyl glucosamine, which was about 39% of total carbohydrate. The relative amount of lipid to protein determined by vanillin phosphoric acid method was 32.5% and its major component was phospholipid, which was about 70% of total lipid. The physicochemical properties of C127 mammalian cell-derved r-HBsAg are similar to those of p-HBsAg, suggesting that the r-HBsAg can be used in developing a new preventive vaccine against hepatitis B.

• The Journal of the Korea Contents Association
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• v.15 no.9
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• pp.509-526
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• 2015

The study investigated the N-Screen strategies of paid digital contents of foreign newspapers belonging to advanced companies: USA, United Kingdom, and Japan. This research employed the time to launch paid digital content business, whether or not to segment market, and 4p marketing mix strategies for analyzing the strategies. The results show that the strategies, starting around the year 2000, had experience chasm, and then revived since the year 2010, when the penetration rate of mobile devices proliferated. Most newspapers still supply contents under the assumption that they paly in a mass market. The items of paid digital contents are mainly composed of e-paper and digital contents. Regarding the price strategy, soft paywall and combination paywall are largely used. Distribution strategy is subject to whether or not to discriminate the path of digital contents. As for promotion strategy, bundling strategy with newspaper is mostly utilized. The difference between western countries and Japan was found in term of the free trial service.

• Journal of Korean Public Health Nursing
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• v.17 no.1
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• pp.144-152
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• 2003

The purpose of this study is to identify the prevalence rate of hypertension and diabetes a in the urban communities and to provide the basic data for development of health promot The subjects of this study were 526 people over the age of 20, living in Seoul. Data for this collected from June 19, 2001 to September 25, 2001. The results of this study are as follows: 1. The subject group is comprised of males, $57.4\%$ and females, $42.6\%$. Their ages range from 20 to 89, and the weights from 40 to 94 kilograms. 2. In the systolic blood pressure, $36.1\%$ showed high, $56.5\%$ normal. and $7.4\%$ low. In the diastolic blood pressure, $50.5\%$ showed high, $48.9\%$ normal, and $1.7\%$. low. In the blood glucose, $70.3\%$ showed normal. $27.9\%$ high, and $1.7\%$. low. 3. There was a significant difference in the mean(SD) of the systolic blood pressure by age(p=.017) and weight(p=.005). Another significant difference was found in the mean (SD) of the diastolic blood pressure by age(p=.006) and weight(p=.007). There was a significant difference in the mean(SD) of the blood glucose by sex and age(p=.001). 4. There were significant correlations between the blood pressure and the blood glucose and the sex, age and weight. 5. The multiple regression analysis showed that the age and weight explained $9.9\%$ of the systolic blood pressure, that the weight and age explained $7.1\%$ of the diastolic blood pressure, and that the age and systolic blood pressure explained $7.0\%$ of blood glucose. The results were useful in developing health promotion programs. This study suggests that a further study be needed.

• Journal of Korean society of Dental Hygiene
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• v.7 no.3
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• pp.259-271
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• 2007

AIDS is increasing by geometric progression in the world with more seriousness and HIV/AIDS is still spreading. Thus, the study enforced a self-administered survey with 526 dental hygiennics students to carry out an upright recognition and education on AIDS by grasping knowledge and attitude of dental hygienics students who will work at dental hospitals or clinics after graduating in AIDS. As the result, the study got the following conclusion. 1. 51.1% of the students got knowledge on AIDS and the majority out of them got its knowledge through school(35.2%) or mass media(10.8%). 2. The average score of dental hygienics students' knowledge in AIDS was 87.67: The first-grade students(86.00), the second-grade students(88.88) and the third-grade students(88.37) (p=0.000). 3. They had an accurate recognition about AIDS' causes, its infection ways and interceptive methods of its infection way in knowledge of AIDS(p=0.000). 4. In their attitudes for AIDS, the average score was $57.43{\pm}$: The first-grade students(56.19), the second-grade students(57.70) and the third-grade students(58.70)(p=0.001). 5. In their attitudes for AIDS, the students($4.09{\pm}0.82$ scores) responded that they would prepare for AIDS' infection of their families(p=0.004) while, on the other hand, the students($4.06{\pm}0.82$ scores) responded that they wanted to avoid PWA's dental treatment(p=0.001). Therefore, it is judged that vocational education as a specialized worker as well as AIDS education should be realized since it was appeared that they had not enough thought as a mature professional worker. 6. Dental hygienics students who would work at dental hospitals or clinics after graduating were in the environment infected in all diseases and so most of the students($4.23{\pm}0.74$) responded that AIDS education should be included in curriculum.

• Journal of Periodontal and Implant Science
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• v.23 no.3
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• pp.526-534
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• 1993

The purpose of this study was to evaluate the changes of the tooth mobility and maximal bite force over 4 weeks following initial therapy on the periodontal disease. Tooth mobility and maximal bite force due to change of viscoelastic property of periodontium were influenced by inflammation of periodontal tissue. 10 patients with the chronic adult periodontitis participated in this study. Each tooth was divided into anterior areas, premolar areas and molar areas. Tooth mobility was tested using Periotest(Siemens Co. Germany) and maximal bite force was evaluated with MPM-3000(Nihon kohden Co. Japan). Tooth mobility and maximal bite force were recorded at the initial examination, 1, 2, 3 and 4 weeks following initial therapy. All data were analyzed statistically. The obtained results were as follows ; 1. The changes of the tooth mobility following initial therapy were generally decreased in maxilla, showing the significant decrease at 1 and 4 weeks on premolar areas (p<0. 05). 2. The changes of the tooth mobility following initial therapy were generally decreased in mandible, however this changes were not statistically significant. 3. The changes of the maximal bite force following initial therapy in maxilla were significantly increased at 3 and 4 weeks on anterior areas, at 4 weeks on premolar areas (p<0. 05). These were decreased at 1 week on molar areas, but generally increasing with time. 4. The changes of the maximal bite force following initial therapy in mandible were significantly increased at 3 and 4 weeks on anterior areas (p<0. 05, p<0. 01). These were decreased at 1 week on premolar but molar areas, and generally increasing with time. 5. As tooth mobility increased, maximal bite force decreased with significance (p<0. 01), and they had high negative correlation on anterior areas but low negative correlation on premolar and molar areas.

• Journal of The Korean Society of Clinical Toxicology
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• v.20 no.2
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• pp.75-81
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• 2022

Purpose: This study sought to compare the characteristics of patients with pathogen-positive and negative cultures, and to investigate factors predicting pathogen-positive culture results in patients of acute poisoning with suspected aspiration. Methods: Consecutive patients with acute poisoning admitted to an intensive care unit between January 2016 and December 2018 were retrospectively studied. Respiratory specimens were collected from the enrolled patients at the time of the suspected aspiration. We compared the characteristics of patients with pathogen-positive and negative culture results and analyzed the causative pathogens. Results: Among the 526 patients, 325 showed no clinical features that could be attributed to aspiration, and 201 patients had clinical features suggestive of aspiration. Of these, 113 patients had pathogen-positive culture, 61 were negative, and the specimens of 27 patients contained poor-quality sputum. In univariate analysis, patients with a positive culture showed a longer time to culture from ingestion (p=0.01), faster heart rate (p=0.01), and higher partial pressure of arterial oxygen to the fraction of inspired oxygen (PaO₂/FiO₂) (p=0.02) than patients with negative culture. Multivariate analysis demonstrated that PaO₂/FiO₂ (adjusted odd ratio, 1.005; 95% confidence interval [CI], 1.002-1.008; p=0.005) was a significant risk factor for pathogen-positive culture. The area under the receiver operating characteristic curve of PaO₂/FiO₂ was 0.591 (95% CI, 0.510-0.669, p=0.05). Gram-negative pathogens (GNPs) were predominant and at least one GNP was observed in 84 (73.3%) patients among those with pathogen positive culture. Conclusion: We failed to find any clinical factors associated with positive culture results. Antibiotics that cover GNPs could be considered when deciding the initial antibiotic regimen at the time of suspected aspiration.

• Journal of Hospice and Palliative Care
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• v.16 no.3
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• pp.175-182
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• 2013

Purpose: Spirituality is an important domain and is related with physical and psychological symptoms in terminal cancer patient. The aim of this study is to examine how patients' spirituality is associated with their physical and psychological symptoms as it has been explored by few studies. Methods: In this cross sectional study, 50 patients in the palliative ward of a tertiary hospital were interviewed. Spiritual well-being, depression, anxiety and pain is measured by Functional Assessment of Chronic-Illness Therapy-Spirituality (FACIT-Sp), hospital anxiety and depression scale (HADS) and the Korean version of the Brief Pain Inventory (BPI-K). The correlations between patients' spiritual well-being and anxiety, depression and pain were analysed. The association between spiritual well-being and age, gender, palliative performance scale (PPS), religion, mean pain intensity, anxiety, depression were assessed by univariate and multivariate regression analyses. Results: Spiritual well-being was negatively correlated with the mean pain intensity (r=-0.283, P<0.05), anxiety (r=-0.613, P<0.05) and depression (r=-0.526, P<0.05). In multivariate regression analysis, spiritual well-being showed negative association with anxiety (OR=-1.03, 95% CI=-1.657~-0.403, P=0.002) and positive association with the existence of religion (OR=9.193, 95% CI=4.158~14.229, P<0.001). Conclusion: In this study, patients' anxiety and existence of religion were significantly associated with spiritual well-being after adjusting age, gender, PPS, mean pain intensity, depression. Prospective studies are warranted.