• 동아시아식생활학회지
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• 제7권4호
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• pp.519-526
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• 1997

Propylene oxide와 반응시켜 치환도가 0-0.2645되는 하이드록시프로필화 감자 전분을 제조하여 이화학적 성질들을 측정하였다. 전분의 하이드록시프로필기 함량은 propylene oxide의 농도에 비례하였다. 하이드록시프로필화 감자 전분의 물결합력은 치환도가 증가할수록 증가하였고 pH는 치환도가 증가할수록 감소하였다. 청가와 요오드 흡착 강도는 치환도가 증가할수록 감소하였다. 시차주사열량계에 의한 호화양상에서는 하이드록시프로필화에 의해 전분의 호화온도가 낮아지는 것으로 나타났다. 하이드록시프로필화에 의해 전분의 상대 결정 화도는 낮아졌고, 전분 입자가 파괴되었음이 X-선 회절도와 주사전자현미경으로 각각 관찰되었다. 이것은 고농도의 하이드록시프로필화가 전분의 무정형 부분뿐만 아니라 결정성 영역에까지 영향을 주었음을 의미하였다.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.1093-1098
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• 2004

An open reading frame encoding CadC, consisting of 526 amino acids, was identified from the upstream region of the Vibrio vulnificus cadBA operon. The deduced amino acid sequences of the cadC were 22 to 78% similar to those reported from other Enterobacteriaceae. Functions of cadC gene on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutant, whose cadC gene was inactivated by allelic exchanges. The results demonstrated that the gene product of cadC contributes to acid tolerance of V. vulnificus, and that its contribution is dependent on prior exposure of cells to moderately acidic pH. The cellular level of cadB and cadA transcripts decreased in the cadC mutant, indicating that CadC exerts its effect on acid tolerance of V. vulnificus by enhancing the expression of cadBA in a pH-dependent manner.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1890-1893
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• 2007

A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.

• 한국보건간호학회지
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• 제31권1호
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• pp.58-70
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• 2017

Purpose: This study was conducted to determine if women's depression is influenced by their self esteem, stress, and social support. Methods: This study was a descriptive survey, and data were collected from the November 1 to December 20, 2015 using a self-reported questionnaire. The measurements were SES for self esteem, and CES-D for depression. A total of 165 middle-aged women participated through convenient sampling. Results: There were significant difference in self-esteem according to education level and monthly income. Additionally, there was an inverse correlation between stress and self esteem, as well as a significant strong correlation between self esteem, stress, social support and depression. Finally, multiple regression showed that self-esteem and stress had significant effects on depression, and their explanatory power was ${\Delta}R2(%)=47.9$ (${\Delta}F=66.526$, p<.001), p<.001). Conclusion: This study confirmed that depression was closely related to self-esteem and stress among middle-aged women. Based on these findings, we suggest that the health policies of reducing depression for middle-aged women should focus on reducing stress and reinforcing their self-esteem.

• 한국식생활문화학회지
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• 제18권6호
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• pp.515-526
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• 2003

The object of this study was to investigate the relationship between the eating habits of high(4th, 5th, 6th) grade elementary school students and parental behavior characteristics(affection, rational guidance, overprotect and neglect). The parenting behavior scale and demographic variables, eating habits and food frequency were administered to the subjects. In this investigation, the subjects were 396 boys and 337 girls in Chunju city in Korea, 50.1% of the mothers was in the age from 35 to 39 years and 57.8% of the mother had an occupation. Data were analyzed by using a SPSS PS package. Significant differences and correlation among variables were determined by the frequency, $x^2-test$ and pearson's correlation coefficient. Eating habits was significantly influenced by mother's employment status, but was not affected by the levels of mother's education and income. Parental behavior characteristics was significantly related to the children's snacks habits(p<0.05, p<0.01), but have no connection with dietary habits and the eating out. The survey revealed higher percentage of the children who had taken affectionate, reasonable and positively reinforced child care, answered they enjoy meals and the other groups answered they did not. Also frequency of having soup, green vegetables, fruits, dairy products had positive relationship with affective, reasonable rearing attitude, but negative relationship with neglective rearing attitude. And the survey showed children who were reared in overprotect had chocolate, candy and the like in large quantities.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.521-526
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• 1998

The physicochmical properties of recombinant hepatitis B surface antigen (r-HBsAg), which was expressed in C127 mammalian cell were studied. Using roller bottle culture in DMEM supplemented with fetal bovine serum, 10-15 mg/L of r-HBsAg was produced with about 31% of purification yield. The purity of r-HBsAg by HPLC was 99.8% and electron microscopic examination showed homogeneous spherical particle with 22 nm in diameter, a morphological characteristic of HBsAg. The density of r-HBsAg by CsCI density gradient method was 1.19g/ml and the isoelectric point by Mono $P^{TM}$ HR 5/20 column was 4.6. The analysis of subunit protein pattern using SDS-PAGE followed by scanning densitometry gave 81.3% of S protein and 18.7% of pre-S protein. fluorophore-assisted-carbohydrate-electrophoresis analysis showed the relative amount of carbohydrate to protein was 1.7% and it smajr component was N-acetyl glucosamine, which was about 39% of total carbohydrate. The relative amount of lipid to protein determined by vanillin phosphoric acid method was 32.5% and its major component was phospholipid, which was about 70% of total lipid. The physicochemical properties of C127 mammalian cell-derved r-HBsAg are similar to those of p-HBsAg, suggesting that the r-HBsAg can be used in developing a new preventive vaccine against hepatitis B.

• 한국콘텐츠학회논문지
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• 제15권9호
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• pp.509-526
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• 2015

본 연구에서는 N-스크린 시대의 미국, 영국, 일본의 유력 신문사의 디지털 콘텐츠 유료화 전략을 비교, 분석했다. 분석의 틀로는 디지털 콘텐츠의 유료화 시점, 시장 세분화 여부, 4P 마케팅 믹스 전략이 활용됐다. 해외 주요 신문사들의 디지털 콘텐츠 유료화 전략은 2000년을 전후로 시작된 후, 2000년 초중반 캐즘을 겪었고 2010년 모바일 기기의 확산으로 본격화된 것으로 나타났다. 분석 대상 신문사 대부분은 상품 측면에서 시장을 세분화하는 모습을 보이진 않았다. 4P 전략의 관점에서 상품 전략의 경우, e-paper와 디지털 콘텐츠를 축으로 프리미엄 서비스가 부가되는 방식으로 전개되고 있었다. 가격 전략은 연성 체계와 혼합 체계가 주로 활용됐다. 유통전략은 디지털 콘텐츠에 대한 세분화 여부에 따라 대별됐다. 디지털 콘텐츠의 유통경로는 '디지털'로 묶어 하나의 대상으로 간주하면 단일 요금제, 그렇지 않고 각각의 단말기마다 세분화 한 경우에는 복합 요금제가 선택됐다. 판촉 전략에서는 종이신문과의 번들링 전략이 주로 채택되는 가운데, 시범 이용을 두고 분석 결과가 서구(미국과 영국)와 일본 간 상이하게 나타났다.

• 한국보건간호학회지
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• 제17권1호
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• pp.144-152
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• 2003

The purpose of this study is to identify the prevalence rate of hypertension and diabetes a in the urban communities and to provide the basic data for development of health promot The subjects of this study were 526 people over the age of 20, living in Seoul. Data for this collected from June 19, 2001 to September 25, 2001. The results of this study are as follows: 1. The subject group is comprised of males, $57.4\%$ and females, $42.6\%$. Their ages range from 20 to 89, and the weights from 40 to 94 kilograms. 2. In the systolic blood pressure, $36.1\%$ showed high, $56.5\%$ normal. and $7.4\%$ low. In the diastolic blood pressure, $50.5\%$ showed high, $48.9\%$ normal, and $1.7\%$. low. In the blood glucose, $70.3\%$ showed normal. $27.9\%$ high, and $1.7\%$. low. 3. There was a significant difference in the mean(SD) of the systolic blood pressure by age(p=.017) and weight(p=.005). Another significant difference was found in the mean (SD) of the diastolic blood pressure by age(p=.006) and weight(p=.007). There was a significant difference in the mean(SD) of the blood glucose by sex and age(p=.001). 4. There were significant correlations between the blood pressure and the blood glucose and the sex, age and weight. 5. The multiple regression analysis showed that the age and weight explained $9.9\%$ of the systolic blood pressure, that the weight and age explained $7.1\%$ of the diastolic blood pressure, and that the age and systolic blood pressure explained $7.0\%$ of blood glucose. The results were useful in developing health promotion programs. This study suggests that a further study be needed.

• 한국치위생학회지
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• 제7권3호
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• pp.259-271
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• 2007

AIDS is increasing by geometric progression in the world with more seriousness and HIV/AIDS is still spreading. Thus, the study enforced a self-administered survey with 526 dental hygiennics students to carry out an upright recognition and education on AIDS by grasping knowledge and attitude of dental hygienics students who will work at dental hospitals or clinics after graduating in AIDS. As the result, the study got the following conclusion. 1. 51.1% of the students got knowledge on AIDS and the majority out of them got its knowledge through school(35.2%) or mass media(10.8%). 2. The average score of dental hygienics students' knowledge in AIDS was 87.67: The first-grade students(86.00), the second-grade students(88.88) and the third-grade students(88.37) (p=0.000). 3. They had an accurate recognition about AIDS' causes, its infection ways and interceptive methods of its infection way in knowledge of AIDS(p=0.000). 4. In their attitudes for AIDS, the average score was $57.43{\pm}$: The first-grade students(56.19), the second-grade students(57.70) and the third-grade students(58.70)(p=0.001). 5. In their attitudes for AIDS, the students($4.09{\pm}0.82$ scores) responded that they would prepare for AIDS' infection of their families(p=0.004) while, on the other hand, the students($4.06{\pm}0.82$ scores) responded that they wanted to avoid PWA's dental treatment(p=0.001). Therefore, it is judged that vocational education as a specialized worker as well as AIDS education should be realized since it was appeared that they had not enough thought as a mature professional worker. 6. Dental hygienics students who would work at dental hospitals or clinics after graduating were in the environment infected in all diseases and so most of the students($4.23{\pm}0.74$) responded that AIDS education should be included in curriculum.

• Journal of Periodontal and Implant Science
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• 제23권3호
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• pp.526-534
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• 1993

The purpose of this study was to evaluate the changes of the tooth mobility and maximal bite force over 4 weeks following initial therapy on the periodontal disease. Tooth mobility and maximal bite force due to change of viscoelastic property of periodontium were influenced by inflammation of periodontal tissue. 10 patients with the chronic adult periodontitis participated in this study. Each tooth was divided into anterior areas, premolar areas and molar areas. Tooth mobility was tested using Periotest(Siemens Co. Germany) and maximal bite force was evaluated with MPM-3000(Nihon kohden Co. Japan). Tooth mobility and maximal bite force were recorded at the initial examination, 1, 2, 3 and 4 weeks following initial therapy. All data were analyzed statistically. The obtained results were as follows ; 1. The changes of the tooth mobility following initial therapy were generally decreased in maxilla, showing the significant decrease at 1 and 4 weeks on premolar areas (p<0. 05). 2. The changes of the tooth mobility following initial therapy were generally decreased in mandible, however this changes were not statistically significant. 3. The changes of the maximal bite force following initial therapy in maxilla were significantly increased at 3 and 4 weeks on anterior areas, at 4 weeks on premolar areas (p<0. 05). These were decreased at 1 week on molar areas, but generally increasing with time. 4. The changes of the maximal bite force following initial therapy in mandible were significantly increased at 3 and 4 weeks on anterior areas (p<0. 05, p<0. 01). These were decreased at 1 week on premolar but molar areas, and generally increasing with time. 5. As tooth mobility increased, maximal bite force decreased with significance (p<0. 01), and they had high negative correlation on anterior areas but low negative correlation on premolar and molar areas.