• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.215-220
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• 2008

Recently, there has been a great deal of interest in the applications of plant-based extracts to both cosmetic and medicinal industries. The objective of this study was to investigate the anti-inflammatory and antimicrobial effect of P. rigida extracts by water and ethyl acetate. Anti-inflammatory and anti-microbial effect of P. rigida extracts by water and EtOAc were investigated by using nitrite scavenging ability, nitric oxide production and anti-microbial ability. In the test of nitrite scavenging ability, P. rigida extracts by water and EtOAc showed 88.7% and 99% at 100 ppm concentration, respectively. The cell viability was measured using the MTT assay at 24 hours after P. rigida extracts as shown in over 80%. Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. NO productions in LPS and P. rigida extracts stimulated group were decreased in a concentration and were dependent on time as compared with LPS stimulated. The water extracts showed the highest inhibition at the 100 ppm concentration. In anti-microbial activity test, the water extract with 3.0 mg/disc resulted in the clear zone of 14 mm, and ethyl acetate with that of 15 mm for Staphylococcus aureus. However, P. rigida extracts didn't show any growth inhibitory effect on Esherichia coli. These results indicate that the extracts of P. rigida have anti-inflammatory activities as a cosmeceuticals.

• The Korean Journal of Ecology
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• v.26 no.2
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• pp.59-64
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• 2003

We examined the effects of fertilization [control (C), 200 kg N ha^{-1} + 25 kg P ha^{-1}$ (LNP), and 400 kg N $ha^{-1} + 50 kg P ha^{-1}$ (HNP)] on fine root (＜ 2 mm diameter) dynamics using monthly soil coring method in a 39-year-old Pinus rigida plantation of central Korea. The average fine root biomass (live + dead) (kg $ha^{-1}$ $\pm$ SE) during the first growing season for C, LNP, and HNP was 1301 $\pm$ 54, 1084 $\pm$ 47, and 1328 $\pm$ 22, respectively. The fine root production (kg $ha^{-1}$ $\pm$ SE) was 2394 $\pm$ 128 for C, 2048 $\pm$ 101 for LNP, and 2768 $\pm$ 150 for HNP, respectively. Over the same period, fertilization treatments had impact on N and P concentrations of live fine root. Nitrogen and P inputs (kg $ha^{-1}$ $yr^{-1}$) into the soil through fine root turnover for C, LNP, and HNP were 16.6 and 0.9, 17.2 and 0.9, and 24.1 and 1.6, respectively. There were no significant differences in fine root biomass and production during the first growing season after fertilization. However, fertilization increased fine root N and P concentrations, and in consequence resulted in increased N and P inputs into soil through fine root turnover.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.455-462
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• 2013

The objectives of this study were to compare soil physical and chemical properties and early growth of Pinus densiflora and Larix kaempferi seedlings in harvested Larix kaempferi and Pinus rigida stands. Study stands were located in harvested L. kaempferi (Yeongju) and Pinus rigida (Wonju) plantations. Seedlings of L. kaempferi (1-1) and P. densiflora (1-0) were planted in April, 2008, Yeongju. Also, seedlings of L. kaempferi (1- 1) and P. densiflora (1-1) were planted with the density of 3,000 seedlings/ha in April, 2010, Wonju. We analyzed soil physical and chemical properties and the relative growth rate of seedlings using diameter at root collar and seedling height. Soil organic matter, total nitrogen and available phosphorus concentrations in harvested P. rigida stand were significantly higher than those in harvested L. kaempferi stand. The relative growth rate of P. densiflora seedlings with former stands were not significantly different. The relative growth rate of diameter at root collar of L. kaempferi seedling in harvested P. rigida stands were significantly higher than that in harvested L. kaempferi stand. Higher concentrations of organic matter, total nitrogen and available phosphorus in harvested P. rigida stand was probably positively affect to the early growth of seedlings.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.319-327
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• 1993

The objectives of this study were to isolate ectomycorrhizal fungi associated with roots of Pinus rigida ${\times}$ P. taeda trees planted in Chonbuk and Daejon locations and to identify them, and to observe the morphology of mycorrhizae formed by the isolated fungi. Pinus rigida ${\times}$ P. taeda seedlings formed ectomycorrhizae with Rhizopogon rubescens, Rhizopogon sp. and Suillus pictus isolated from the roots of mature Pinus rigida ${\times}$ P. taeda trees, Pinus rigida ${\times}$ P. taeda seedlings formed ectomycorrhizae with Pisolithus tinetorius too. The root surface of inoculated seedlings was covered with mycelia, and Hartig net were well developed in those roots. The size of vacuole was small, and the protoplasm was planty in ectomycorrhizal cell, compared to nonmycorrhizal cell. But the size of nuclei are not changed even in ectomycorrhizal cell.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.39-50
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• 1979

Twelve Pinus taeda were used as mother trees and one P. rigida plus tree was used as pollen tree for 12 cross combinations. Nine P. densiflora plus trees(4 mother and 7 pollen trees) were used as parents for 11 cross combinations. Those parents and 10 progenies were analyzed for LAP of ${\times}$P. taeda rigida hybrid and P. densiflora and for peroxidase of ${\times}$P. taeda rigida. The analysis, based on the banding patterns, indicate three alleles for LAP-A locus(A1, A2, A3) and two alleles for LAP-B locus (B1, B2) in ${\times}$P. taeda rigida hybrids. Chi-square test on the segregation for progenies did not show significant differences. The results indicated good agreement with monohybrid Mendelian inheritance. Independence test for occurrence frequency of 2 alleles(LAP-A3, LAP-B2) illustrated that there is neither linkage nor repulsion relationship between LAP-A3 and LAP-B2 alleles. Three band at LAP-A locus were always exhibited from all parents and their progenies of P. densiflora. However, the occurrence of two bands at LAP-B locus was variable, one bands assumed as homozygous alleles(B2/B2) and two bands as heterozygous alleles(B1/B2). The segregation ratio for progenies of P. densiflora suggested that LAP-B locus may be controlled by two alleles(B1 and B2). Three Peroxidase loci(Px-A, Px-B, Px-C) assumed to be controlled by allozyme in ${\times}$P. taeda rigida hybrid. The Px-B and Px-C loci could not find out the variations from banding patterns of parents and their progenies, while the Px-A locus showed the variations of occurrence frequency by two bands. The segregation ratio for A1/A2 at LAP-A locus suggest that the peroxidase allozymes of ${\times}$P. taeda rigida hybrid appeare to be monomeric products; that is, Px-A locus may be controlled by two alleles (A1 and A2).

• Journal of the Korean Wood Science and Technology
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• v.36 no.1
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• pp.12-20
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• 2008

Radial and vertical variations of relative crystallinity and crystallite width of cellulose within stems of three softwoods (Pinus densiflora S. et Z., P. koraiensis S. et Z, P. rigida Mill.) grown in Korea were examined by an X-ray diffraction method. The mean of relative crystallinity was 61.7% in P. koraiensis, 60.6% in P. densiflora and 49.4% in P. rigida. The degree of crystallinity in earlywood and latewood increased with the age from pith to about 10~15 years, and then remained almost constant value. The relative crystallinitiy of latewood was slightly higher than that of earlywood. The relative crystallinity in P. densiflora was a little lower at the base of stem, but no significant difference by height was shown in P. koraiensis and P. rigida. The crystallite widths in the stems were 2.8 to 3.0 nm, but were not significantly different in earlywood and latewood by height. In conclusion, the relative crystallinity appeared to be a useful index for separating juvenile wood from adult wood in the softwoods of P. densiflora, P. koraiensis, and P. rigida grown in Korea.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.329-340
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• 1996

The extracts of selected plants and analyzed phenolic compounds were used to study the effects of alleloKDICicals on seed germination and seedling growth. HPLC analysis of the aqueous extracts of seven species identified 15 phenolic compounds including caffeic acid. Among them, protocatechuic acid was detected at 65.87ppm and 6.84ppm, in Erigeron canadensis and Pinus rigida, respectively. And the extract of P. rigida showed the strongest inhibitory effect on seed germination. The extract of P. rigida leaves significantly inhibited germination and radicle growth of Raphanus sativus var. hortensis for. acanthiformis in direct proportion to concentration. However, germination of Cassia mimosoides var. nomame was stimulated by the treated extracts at the same concentrations, but root growth was inhibited at high concentrations. Except chlorogenic acid, eleven of the twelve phenolic compounds inhibited the germination of R. sativus var. hortensis for. acanthiformis. In the case of C. mimosoides var. nomame, some phenolic compounds such as chlorogenic acid, vanillic acid, protocatechuic acid, ferulic acid, gallic acid and ${\rho}-coumaric$ acid stimulated germination, while the others reduced it.

• Ecology and Resilient Infrastructure
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• v.2 no.4
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• pp.291-295
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• 2015

The insect community structures between a plantation of Pinus rigida and a secondary forest of Quercus monglica were compared to find out one of the ecological roles of a forest planted in 1960s in a suburban area of Seoul, Korea. We collected the insect samples biweekly from September to November in 2014 by using 5 pitfall traps in both forests. The results of analyzing the community structure index of insects in both forests of P. rigida and Q. Mongolica showed that the species richness was a little higher and species evenness was a little lower in P. rigida plantation, and species diversity and dominance were similar in the two forests. The analysis results of insect community structure at the two forests did not show any significant difference. We conclude that the plantation of P. rigida over 40-50 years could sufficiently perform an ecological function as an insect habitat.

• The Korean Journal of Mycology
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• v.10 no.3
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• pp.125-129
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• 1982

Of about 70 different mushrooms (potential ectomycorrhizal fungi) collected from forest floor of a 24-year-old Pinus rigida rigida ${\times}$ taeda stand, four species have not been reported in Korea. They are Inocybe fastigiata, Phylloporus bellus, Lactarius glaucescens, and Lactarius subvellereus. The Phylloporus bellus has been incorrectly identified in Korea as Phylloporus rhodoxanthus. Taxonomic descriptions for these four mushrooms are reported in this paper. The collections cited are deposited in the herbarium of Institute of Agricultural science O.R.D.

• Journal of Korean Society of Forest Science
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• v.4 no.1
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• pp.33-38
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• 1965

It has already been noted that some tree species, denoted as tolerant species, endure to behavior shadiness in growth than those species, denoted as intolerant species, since this fact of tolerance must be involved in silvicultural practice, the present author, with pinus rigida, pinus Thunbergii, and Buxus koreana, investigated the relation of degree of shadiness to soil moisture condition. Because tolerant and intolerant trees must be considered from the standpoint of their ability to use any of the factors that are likely to be at low concentration in the dense or open forest. The observed results may be summarized as follows : 1. The ability of tolerance of the species to shadiness is differently concluded by the base with which the analysis was made, i.c. Height, growth, diameter growth, T-R ratio or dry matter increase and so on. Regarding this point root collar increment or dry matter increase were considered more reasonable bases for discussing the tolerability. 2. When total length growth, including both stem and root, is taken as standard for the comparison of the ability of tolerance for each species, higher soil moisture condition and thicker shadeniss lessened the ability of pinus rigida, seedlings. Pinus Thunbergii, showed. However more moisture resistant than pinus rigida. Buxus koreana, seemed to have stronger ability of tolerance than any others. 3. When total dry matter content taken as standard, the results obtained are given in Fig, 3. In pinus rigida, the soil moisture supplemented the lack of light.