• The Korean Journal of Food And Nutrition
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• v.6 no.2
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• pp.67-72
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• 1993

Cultural conditions and carbon sources affecting the productivity of cellulolytic enzymes of Pleurotus ostreatus 201 were examined in synthetic media. The optimum cultural temperature and initial pH for the production of enzymes were $25^{\circ}C$ and 5.5 in avicelase, and 3$0^{\circ}C$ and 5.0 In CMCase, and 3$0^{\circ}C$ and 6.5 in B-glucosidase. Among carbon sources, cellulose powder was the best for the production of avicelase, and Na-CMC for CMCase, and cellobiose for $\beta$-glucosidase. The optimum concentration of cellulose powder was 1.0% (w/v), and glucose depressed the production of enzymes remarkably.

• Korean Journal of Microbiology
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• v.24 no.3
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• pp.323-328
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• 1986

To clarify the effects of several carbohydrates on the biodegradation of lignin by Pleurotus ostreatus. The strain was cultured on the media formulated with lignin and carbohydrates such as cellulose, xylan, collobiose, glucose and xylose, which was added individually. The culture mixtures grown 36 days were filtered and then estimated the degree of lignin biodegradation. It was found that the growth of P. ostreatus was stimulated and the depoly-merization was also increased by the addition of carbohydrates. When the carbohydrates were not added, polymerization was apparent in stead of depolymerization. In the case of glucose as an added carbohydrate, the content of lignin by the nitrosolignin method was greatly (about 7.4 times) decreased than control which contains lignin as a carbon source. The peak of lignin at 280nm in UV spectra was decreased about 27% after 27 days of culture. As results, it was assumed that lignin biodegradation was correlated to the carbohydrates and especially glucose was very significant role in lignin degradation.

• Journal of Practical Agriculture & Fisheries Research
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• v.12 no.1
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• pp.21-27
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• 2010

The objective of this study was to determine the effects of "Green-one" organic nutrient on mycelial growth and fruiting of P. ostreatus. The dilution concentrations of "Green-one" was treated as follows. There was control, 100, 200, 400 concentrations. That treatments were treated with step of each mycelial growth step. The best of growth steps was mycelial scratching step. At that time, DPI(Day required for primordial formation after inoculation) was shortened for 1 day. Valid germination stipe are 15 pieces, 3 pieces more than control. Stipe length and stipe diameter was long each 4mm, 3mm more than control. Pileus size is shortened than control. Yields per one bottle(g/850cc) was 146g increased 6.5% than control 137g/850cc.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.85-89
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• 2009

For the purpose of the methodological development to convert the food wastes into resources, we have attempted to culture the mushroom hypha, Pleurotus ostreatus. The food-wastes were mixed with distilled water, and the mixture was autoclaved to produce fluid, which was centrifugated and used as the growth media. Concentrations of the food wastes extracts were prepared with 10, 20, 30, 40, and 50%(W/V), and the initial pH were set variously with 4, 5, 6, and 7. These were cultured for 9 days at the temperature of $25^{\circ}C$ and the rotation rate of 120 rpm. The result is that the fluid form of the mushroom hypha have been grown best at the concentration of 30% and the optimal pH was 5 and 6.

• Korean Journal of Microbiology
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• v.14 no.3
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• pp.99-99
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• 1976

Some properties of xylanase produced by Pleurotus ostreatus during its gorwth in a rice straw medium were investigated. The results are summarized as follows : 1) The optimum pH of xylanase was 5.0 and the stable pH ranged from 4.5 to 6.0. 2) The optimum temperature for the xylanse was around $50^{\circ}C$ and the xylanse activity was completely lost in 10 minutes at $70^{\circ}C$ 3) The activity of xylanse was inhibited by managanous ion, but was increased by other metalic ions. Especially K, Mg and Ca ions considerably increased the activity.

• Korean Journal of Microbiology
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• v.14 no.3
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• pp.93-104
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• 1976

Some properties of xylanase produced by Pleurotus ostreatus during its gorwth in a rice straw medium were investigated. The results are summarized as follows : 1) The optimum pH of xylanase was 5.0 and the stable pH ranged from 4.5 to 6.0. 2) The optimum temperature for the xylanse was around $50^{\circ}C$ and the xylanse activity was completely lost in 10 minutes at $70^{\circ}C$ 3) The activity of xylanse was inhibited by managanous ion, but was increased by other metalic ions. Especially K, Mg and Ca ions considerably increased the activity.

• Korean Journal of Microbiology
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• v.38 no.1
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• pp.54-56
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• 2002

Induction of laccase isozymes under acidic stresses were determined in Trametes versicolor, Pleurotus ostreatus and Ganoderma lucidum isolated in Korea, and in Lentinus squarrosulrs isolated in Thai. When cultures of these fungi were transferred to acidic liquid media (pH 3.0-4.0), the activities of secreted extralcellular laccases were increased 60% and 400% in T. versicolor and G. lucidum respectively. However, there was no such induction in L. squarrosulus or P. ostreatus. In L. squarrosulus, different laccase isozymes in the electrophoretic mobilities were induced under acidic conditions.

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1271-1283
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• 2007

A fibrinolytic protease (PoFE) was purified from the cultured mycelia of the edible oyster mushroom Pleurotus ostreatus, using a combination of various chromatographies. The purification protocol resulted in an 876-fold purification of the enzyme, with a final yield of 6.5%. The apparent molecular mass of the purified enzyme was estimated to be 32 kDa by SDS-PAGE, fibrin-zymography, and size exclusion using FPLC. The optimal reaction pH value and temperature were pH 6.5 and $35^{\circ}C$, respectively. PoFE effectively hydrolyzed fibrinogen, preferentially digesting the $A{\alpha}$-chain and the $B{\beta}$-chain over the ${\gamma}$-chain. Enzyme activity was enhanced by the addition of $Ca^{2+},\;Zn^{2+},\;and\;Mg^{2+}$ ions. Furthermore, PoFE activity was potently inhibited by EDTA, and it was found to exhibit a higher specificity for the chromogenic substrate S-2586 for chymotrypsin, indicating that the enzyme is a chymotrypsin-like metalloprotease. The first 19 amino acid residues of the N-terminal sequence were ALRKGGAAALNIYSVGFTS, which is extremely similar to the metalloprotease purified from the fruiting body of P. ostreatus. In addition, we cloned the PoFE protein, encoding gene, and its nucleotide sequence was determined. The cDNA of cloned PoFE is 867 nucleotides long and consists of an open reading frame encoding 288 amino acid residues. Its cDNA showed a high degree of homology with PoMEP from P. ostreatus fruiting body. The mycelia of P. ostreatus may thus represent a potential source of new therapeutic agents to treat thrombosis.

• Journal of Mushroom
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• v.10 no.2
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• pp.74-82
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• 2012

Sixty strains of Pleurotus ostreatus, white-rot fungi, were screened for production ability of their lignocellulolytic enzymes to selectively wood degradation. That results were shown that all of screened strains were produced lignocellulolytic enzymes on 2nd screening liquid culture medium. However, cellulase activity of selected six strains of P. ostreatus was low in avicel-yeast-peptone liquid culture medium. In the case of xylan degrading enzyme, No. 6 and No. 38 strains produced a xylanase(above 1.0U/ml) and a 1,4-${\beta}$-xylosidase (above 0.15 U/ml). Examination of the ligninolytic enzyme profiles of selected thirteen strains of the P. ostreatus, in the presence of Remazol Brilliant Blue R(RBBR), were observed that laccase(Lac) activity were earlier reached maximum level(0.8-2.0 U/ml) and then Mn-dependent peroxidase (MnP) were reached maximum level(0.5-1.5 U/ml) in glucose-yeast-peptone(GYP) medium. On the other hand, activity of lignin peroxidase(LiP) was not detected in this medium. I selected the No. 42 strain of P. ostreatus produced high levels of Mn-dependent peroxidase and laccase based on the screening method.

• Journal of Mushroom
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• v.14 no.4
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• pp.174-178
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• 2016

Simple sequence repeats (SSR), also referred to "microsatellites" consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from 'Heuktari' and 'Miso', which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of 'Heuktari' and 'Miso' were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the 'Heuktari' and 'Miso' genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in 'Heuktari' and 'Miso' were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.