• 한국작물학회지
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• 제60권2호
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• pp.239-247
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• 2015

This experiment was conducted to obtain the have higher contents of pharmaceutical constituents as well as higher yield from colchicine induced diploid and tetraploid extracts of Platycodon grandiflorum. In order to determine the biological activity, this study was focused to evaluate the cytotoxicity, antimicrobial on the bronthus disease bacteria, antioxidant enzyme activity of diploid and tetraploid extracts in P. grandiflorum. The activities of antioxidant enzyme according to different solvent extracts were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The cytotoxicity of methanol extracts of P. grandiflorum showed significant differences between tetraploid and diploid. That is, the cytotoxic effect against human cancer cell was higher in tetraploid than in diploid. At all extracts concentration, tetraploid samples showed high toxicity and the $IC_{50}$ (concentration causing 50% cell death) value showed the highest on HCT-116 cell ($105.91{\mu}g/mL$), and exhibited significant activity against the Hep 3B cell ($140.67{\mu}g/mL$), SNU-1066 cell ($154.01{\mu}g/mL$), Hela cell ($158.37{\mu}g/mL$), SNU-601 cell ($182.67{\mu}g/mL$), Calu-6 cell ($190.42{\mu}g/mL$), MCF-7 cell ($510.19{\mu}g/mL$). Antimicrobial activities of diploid P. grandiflorum were relatively low compared to tetraploid P. grandiflorum on most of the bacterial strains. In tetraploid P. grandiflorum, K. pneumoniae showed the clear zone formation (18~19 mm) of growth inhibition, followed by the clear zone formation of 13~15 mm on C. diphtheria and S. pyogenes. The antimicrobial activities in diploid P. grandiflorum were the highest on K. pneumonia (14~15 mm), and showed the clear zone formation of 11~12 mm on C. diphtheria and 12~13 mm on S. pyogenes. The antimicrobial activity is thought to look different depending on the bacterial strains and the polyploidy of P. grandiflorum. The root extract of P. grandiflorum had the highest (97.2%) SOD enzyme activity in ethyl acetate partition layer of tetraploid while water partition layer of diploid showed the lowest (48.6%) SOD enzyme activity. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all partition layers except butyl alcohol. The activities of APX and POD showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all fraction solvents except water layer. These results indicate that the tetraploid P. grandiflorum can be used as a source for developing cytotoxic agent and antimicrobials which can act against bronchus diseases bacterial strains.

• 한국식품영양학회지
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• 제26권4호
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• pp.759-765
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• 2013

본 연구는 여러 가지 기능성과 생리활성을 가지고 있는 도라지를 활용하고자 도라지 분말을 0%, 1%, 3%, 5% 첨가하여 도라지 쿠키를 제조한 후 그 품질 특성을 측정하였다. 도라지 분말의 첨가량이 증가할수록 쿠키의 수분 함량과 pH가 낮아졌다. 쿠키의 색도는 도라지 분말의 첨가량이 많아질수록 명도(L)는 감소했으며, 적색도(a)와 황색도(b)는 증가하였다(p<0.001, p<0.01). 쿠키의 퍼짐성 지수는 도라지 분말의 첨가량이 많아질수록 높아졌으나, 도라지 분말의 첨가량과 쿠키의 퍼짐성 간에는 통계적으로 유의한 차이를 나타내지 않았다. 도라지 쿠키의 경도는 첨가량의 증가와 함께 낮아지는 것으로 나타났다. 도라지 쿠키의 관능적 특성은 1% 첨가군이 색과 맛, 조직감, 전체적인 기호도가 가장 높았으며, 풍미는 무첨가군이 가장 높았고, 도라지 분말 첨가량이 증가할수록 낮았다(p<0.001).

• 한국자원식물학회지
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• 제35권6호
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• pp.713-719
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• 2022

Platycodon grandiflorum and Glycyrrhiza uralensis contain several bioactive compounds, such as saponin, oleanolic acid, and flavone. P. grandiflorum and G. uralensis have traditionally been used to treat disorders related to blood pressure, diabetes, and counteracting poison, and they have antinociceptive and antiinflammatory properties. However, the validity of complex saponin's vasodilatory effect has not been scientifically investigated. Therefore, this study explores the vasodilatory effect of complex saponin extracted from P. grandiflorum and G. uralensis mixture extract on rabbit carotid arteries. To this end, arterial rings with intact or damaged endothelium were used in an organ bath experiment and contracted by endothelin. Complex saponins, the major active constituents of P. grandiflorum and G. uralensis mixture extract, exhibited a moderate vasodilatory effect on the rabbit's basilar arteries. Therefore, treatment with complex saponin extracted from P. grandiflorum and G. uralensis mixture extract may selectively accelerate cerebral blood flow through basilar arterial dilation. Overall, the findings suggest that the extracted complex saponins can serve as vasodilator sources.

• 한국자원식물학회지
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• 제26권3호
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• pp.389-396
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• 2013

The antioxidant enzyme and DPPH radical scavenging activity with variations in drying methods of diploid and tetraploid in Platycodon grandiflorum were determined. Antioxidant enzyme activities were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), and ascorbate peroxidase (APX). The roots of Platycodon grandiflorum were freeze-dried, indoor-dried, hot-air dried, and microwave dried. The root extract of P. grandiflorum have shown the highest SOD enzyme activity of 92% in tetraploid of freeze-dried and indoor-dried while diploid of microwave dried showed the lowest SOD enzyme activity of 47.5%. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all drying methods. The APX activity showed relatively higher values in the root extract of freeze-dried both the diploid and tetraploid, but the difference in comparison with other extracts was not significant. The POX activities according to drying methods of diploid and tetraploid in P. grandiflorum showed relatively high values in freeze-dried and indoor-dried compared with other drying methods, and the POX activity between the diploid and tetraploid was not significant difference in each drying method. The DPPH radical scavenging activity with variation in drying methods of diploid and tetraploid in P. grandiflorum was the highest in the freeze-dried, and was higher in tetraploid than diploid in all the concentrations. In conclusion, the root of P. grandiflorum had the potent biological activities in both diploid and tetraploid. In particular, the tetraploid root of P. grandiflorum showing high antioxidant enzyme activity could be good materials for development of source of functional healthy food.

• Weed & Turfgrass Science
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• 제7권1호
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• pp.72-75
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• 2018

Balloon flower (Platycodon grandiflorum) is one of minor crops in Korea. Only four ACCase inhibiting herbicides are registered for P. grandiflorum and the farmers usually use general but unregistered herbicides which can cause critical phytotoxicity. So, this study was conducted to investigate effects of alachlor and pendimethalin on germination and early growth of P. grandiflorum. To evaluate the effect of alachlor and pendimethalin, two herbicides were treated to the seeds in petri-dish and soil. In the petri-dish, alachlor completely inhibited the germination as well as pendimethalin inhibited the germination slightly but not significantly. In case of soil application, alachlor inhibited germination and plant height of P. grandiflorum significantly as well as pendimethalin inhibited plant height and root length significantly. We showed the possibility of phytotoxicity of alachlor and pendimethalin to P. grandiflorum and strongly suggest that two herbicides should not be used to the cultivation of P. grandiflorum. Unregistered herbicides should not be used for weed management and additional researches for screening of safe herbicide to minor crops should be conducted as soon as possible.

• 한국약용작물학회지
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• 제23권5호
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• pp.363-369
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• 2015

Background : Platycodon grandiflorum radix used as a therapeutic agent for lung and respiratory diseases in traditional Korean medicine. The saponin and sugar content in P. grandiflorum root is dependent on the drying temperature and method. In this study, we investigated saponins and antioxidant contents in dried P. grandiflorum roots either not cut or cut to different lengths. Methods and Results : P. grandiflorum roots were either cut to 0.5, 1.0 or 2.0 cm in length or left uncut. They were dried at $45^{\circ}C$ and the effect of antioxidant substances, antioxidant ability, and saponin content were investigated. P. grandiflorum roots rapidly dried out when cut to short lengths. The saponin contents (platycodin D, polygalacin D, and deapioplatycodin D) was highest in the uncut P. grandiflorum roots. When the cut lengths were long, the levels of polyphenolic compound increased. However, 2,2'-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher in the samples cut to shorter lengths. Conclusions : These results suggested that the saponin and antioxidant content of P. grandiflorum roots could be maximized by controlling the drying rate of the roots via adjustments to the root cutting length.

• 한국약용작물학회지
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• 제24권1호
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• pp.55-61
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• 2016

Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.

• 한국자원식물학회지
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• 제29권3호
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• pp.331-338
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• 2016

The present study aimed to compare and investigate the morphological characteristics and yield components according to ploidy level of diploid and tetraploid Platycodon grandiflorum under vinyl-greenhouse and open field conditions. Plant height of diploid and tetraploid P. grandiflorum was 51.3 ㎝, 54.0 ㎝, respectively. The results revealed that the plants grown in the vinyl-greenhouse showed significantly higher growth compared to those grown in the open field. Regardless of the growing place, diploid and tetraploid of P. grandiflorum showed the rapid elongation of internodes after 4 and 3 internodes respectively and elongation tends to be decreased as entering the flower-bud differentiation period. The starting day of flowering in vinyl-greenhouse cultivation was found to be faster than that of the open field cultivation by 2∼3 days and tended to be delayed by about 5∼6 days in tetraploid P. grandiflorum compared to diploid. Fresh weight of roots from the vinyl-greenhouse cultivation showed a high quantity as 34.2g and 49.4g in diploid and tetraploid P. grandiflorum, respectively and especially tetraploid P. grandiflorum was found to be increased by approximately 44.4% compared to other plots.

• Food Science and Biotechnology
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• 제14권6호
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• pp.841-846
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• 2005

Using high-fat-fed C57BL/6 mice, therapeutic effects of oral administration of aqueous extracts of Platycodon grandiflorum and Acanthopanax senticosus on obesity were evaluated by comparing body and liver weights, feed intake, adipose tissue mass, blood lipid profile, and triglyceride and total cholesterol levels in liver and feces. Obesity was successfully induced in high-fat diet group. P. grandiflorum and A. senticosus were effective in reducing food intake, serum lipid levels, adipose tissue accumulation, and weight. P. grandiflorum extract reduced triglyceride and cholesterol levels in liver by excluding them in feces. A. senticosus extract increased triglyceride level in liver but decreased in feces, and decreased total cholesterol in liver and feces, indicating active ingredient of A. senticosus exert antiobesity effect through mechanism different from that of P. grandiflorum extract. These results suggest aqueous extracts of P. grandiflorum and A. senticosus have synergistic effect for prevention of hyperglyceridemia and obesity.

• 대한본초학회지
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• 제28권2호
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• pp.25-31
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• 2013

Objectives : The purpose of this study was to characterize the effect of the Ethanolic extracts of Platycodon grandiflorum and its permented production the learning and memory impairments induced by scopolamine. Methods : The cognition-enhancing effect of Platycodon grandiflorum and its permented production were investigated using a passive avoidance test, the Morris water maze test and Y-maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine (1 mg/kg, i.p.). Results : The results showed that the Permented Platycodon grandiflorum extract-treated group (100 mg/kg, p.o.) and the tacrine-treated group (10 mg/kg, p.o.) significantly ameliorated scopolamine-induced amnesia based on the Passive avoidance Y-maze test and Water maze test. And these results are same manner in DPPH radical scavenger effect and Acetylcholineseterase inhibition effect. Conclusions : These results suggest that 80% Ethanol extract of fermented P.grandiflorum showed significant anti-amnestic and cognitive-enhancing activities related to the memory processes, and these activities were parallel to treatment duration and dependent of the learning models.