• Journal of Mushroom
• /
• v.3 no.1
• /
• pp.40-44
• /
• 2005

The results of examining selection of optimal mycelial growth condition excellent strain for determining the condition of Pleurotus cornucopiae are as follows. 1) Mycelial growth and density of P. cornucopiae were the highest in the medium of MCM(87.8mm/10d) followed by the order of PDA, YM, MEA and Czapek. 2) Optimal temperature for the mycelial growth and density of P. cornucopiae was shown to be $25^{\circ}C$, but the hyphae were dead at $5^{\circ}C$. The mycelial growth and density of KNAC2003 strain was the highest at $25^{\circ}C$(87.8mm/10d) followed by the order of 25, 30, 20, 35, 10 and $5^{\circ}C$. 3) Optimal pH for the mycelial growth and density was revealed to be 6.0(88.3mm/10d), but the mycelial growth and density were shown to be retarded in above or below pH 6.0.

• Journal of Mushroom
• /
• v.3 no.2
• /
• pp.79-84
• /
• 2005

The research for incubation period, mycelial density, day required for primordial formation after inoculation(below DPI), number of valid stipes, individual weight and accumulation amounts of organic selenium for P. cornucopiae by treating 100, 200, 300, 400, 500(${\mu}g/50g$) of $Na_2SeO_3$ is following. Incubation periods of P. cornucopiae are 20~23 days per each low concentration treatment with $Na_2SeO_3$. Compared to the control which took 22 days of incubation period, it is reduced 1 or 2 days. Mycelial density of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $500{\mu}g/50g$ is very compact. DPI of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $400{\mu}g/50g$ was reduced 1 or 2days, but $500{\mu}g/50g$ was increased 1 day. Number of valid stipes of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $400{\mu}g/50g$ is between 19 and 20. It was increased 1 or 2, as compared to 18 of control, but $500{\mu}g/50g$ was reduced to 1. Individual weight of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $400{\mu}g/50g$ was between 129 and 138g/850cc. It was increased 4.9~12.2% as compared to 123g/850cc of the control but $500{\mu}g/50g$ was 122g/50g. Accumulation amount of organic selenium for P. cornucopiae treated with $Na_2SeO_3$ between 100 and $500{\mu}g/50g$ was $2.73{\sim}8.19{\mu}g/g/dry$. It was increased 55~164 times as the concentration increased when compared to $0.05{\mu}g/g/dry$ of the control. In conclusion, incubation period, mycelial density, DPI, number of valid stipes, individual weight and accumulation amounts of organic selenium for P. cornucopiae by treating 100, ${\sim}400{\mu}g$ of $Na_2SeO_3$ was increased, but $500{\mu}g/50g$ was reduced. So more than $500{\mu}g/50g$ concentration treatments are required research.

• Journal of Mushroom
• /
• v.3 no.2
• /
• pp.85-89
• /
• 2005

The research for incubation period, mycelial density, day required for primordial formation after inoculation(below DPI), number of valid stipes, individual weight and accumulation amounts of organic selenium for P. cornucopiae by treating 600, 700, 800, 900, 1000(${\mu}g/50g$) of $Na_2SeO_3$ is following. Incubation periods of P. cornucopiae are 25~30 days per each treatment with $Na_2SeO_3$. Compared to the control which took 22 days of incubation period, it is increased 3 or 8 days for the treatment of $600{\sim}1000{\mu}g/50g$. Mycelial density of P. cornucopiae treated with $Na_2SeO_3$ between 600 and $1000{\mu}g/50g$ is very similar with control. DPI of P. cornucopiae treated with $Na_2SeO_3$ between 600 and $1000{\mu}g/50g$ was increased 3 or 8 days. Number of valid stipes of P. cornucopiae treated with $Na_2SeO_3$ between 600 and $1000{\mu}g/50g$ was between 10 and 16. It was decreased 2 or 8 as compared to 18 of control. Individual weight of P. cornucopiae treated with $Na_2SeO_3$ between 600 and $1000{\mu}g/50g$ was between 94 and 116g/850cc. It was decreased 5.7~23.5% as compared to 123g/850cc of the control. Accumulation amount of organic selenium for P. cornucopiae treated with $Na_2SeO_3$ between 600 and $1000{\mu}g/50g$ was $9.1{\sim}10.8{\mu}g/g/dry$. It was increased 182~216 times as the concentration increased when compared to $0.05{\mu}g/g/dry$ of the control. In conclusion, incubation period, mycelial density, DPI, number of valid stipes, individual weight and accumulation amounts of organic selenium for P. cornucopiae by treating $600{\sim}1000{\mu}g/g$ of $Na_2SeO_3$ was decreased. So that the optimal treatment was less $400{\mu}g/g$ than $600{\sim}1000{\mu}g/g$.

• The Korean Journal of Mycology
• /
• v.14 no.2
• /
• pp.141-148
• /
• 1986

The optimal conditions for high yields of mycelial protoplasts from P. cornucopiae were established. The concentraion of enzyme system containing Novozym 234, ${\beta}-D-glucanase$ and ${\beta}-glucuronidase$ was $5mg\;ml^{-1}$ each. The osmotic stabilizer most effective for protoplast isolation was O.6 M sucrose. The optimal reaction time of mycelium with the lytic mixture was 90 min in a shaking condition at 120 strokes $min^{-1}$. When the myelium of P. cornucopiae was cultured for 4 days on mushroom complete medium at $28^{\circ}C$, the formation of protoplast was effective. When the pH of the digestion mixture with O.6 M sucrose as stabilizer varied between pH 4.0 and 7.0, the production of protoplasts was effective in phosphate buffer (pH 6.2) and Na-maleate buffer (pH 5.0). Generally, phosphate buffer was more effective for protoplast isolation than Na-maleate buffer, but 0.6 M sucrose osmotic stabilizer without adjusting pH was most effective. Using these conditions, protoplasts from P. cornucopiae were obtained at a ratio $1{\times}10^7\;ml^{-1}$.

• Korean Journal of Microbiology
• /
• v.31 no.2
• /
• pp.141-147
• /
• 1993

Plasmid DNAs were detected from the mitochondrial fraction of four strains of whiterot fungus, Pleurotus ostreatus. The size of the plasmids were 10.2 and 7.2 kb in strain NFFA 2, 10.2 kb in NFFA 4001, 11.2 kb in NFFA 4501, and 10.2 and 11.2 kb in KFCC 11635. The two strains,NFFA 2ml and NFFA 2m2, which are mutant derivatives of NFFA 2, did not contain any plasmids. The cleavage by proteinase K indicated that these plasmids have DNA ends associated with proteins. In digestion with proteinase K all the plasm ids remained resistant to lambda exonuclease which hydrolyzes DNA from 5' ends and were sensitive to exonuclease III which hydrolyzes DNA from 3' ends. This suggests that the plasmids are linear double-stranded DNA and the terminal proteins are covalently linked to 5' ends of plasm ids. In order to find relationship between these plasmids, hybridization of plasm ids by each separate plasmid DNA was done. The result indicated that the plasmids can be classified into at least 3 groups. Plasmids of group I were present in all the P ostreatus. More mitochondrial plasmids were detected in P cornucopiae. P ,florida, P pulmonarius, P sajor-caju, and P spodoleucus. The size of plasmids ranged between 7.2 kb and 14 kb. All the species except P cornucopiae contained plasmids of approximately 10 kb which hybridized with the 10.2 kb plasmid (group I) of P ostreatus NFFA 2.

• The Korean Journal of Mycology
• /
• v.16 no.2
• /
• pp.95-100
• /
• 1988

Isozyme band patterns were investigated by isoeletric focusing Esterase and Leucine amino peptidase of Pleurotus spp. in Korea. Esterase patterns of mycelia and fruitbody were distinquished. However, those of primordia, cap and stem were similar. Interspecies differences of Pleurotus ostreatus, P. cornucopiae and P. florida of Esterase zymogram were found. Species identification by electrophoretic zymogram may be a role as an additional taxonomic tool.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.6
• /
• pp.836-841
• /
• 2014

Physiological activities of extracts form Pleurotus cornucopiae were examined. DPPH radical scavenging activity of water extract was 70.22 percent at 4 mg/mL, which was the highest among all extracts. Total polyphenol content of water extract was 26.34 mg/g, which was the highest value for all extraction conditions. Superoxide anion radical scavenging activities ranged from 50.17 to 80.56 percent at 1, 2, and 4 mg/mL, which were higher than the ascorbic acid activity (56.19% at 10 mg/mL) (P<0.05). ACE inhibitory activities were higher in ethanolic extracts than in water extracts. Nitrite-scavenging abilities under acidic conditions (pH 1.2 and pH 3.0) were the most effective among all the extracts. These results will be useful for understanding the physiological activities of Pleurotus cornucopiae.

• Journal of Microbiology
• /
• v.34 no.1
• /
• pp.38-39
• /
• 1996

The internal regions of nuclear small subunit rRNA from 6 plaeurotus species and 5 Pleurotus ostreatus strains were amplified by PCR and sequenced. The DNA sequences of 8 Pleurotus strains (P. ostreatus NFFA2, NFFA4501, NFFA4001, KFFA4001, KFCC11635, P florida, P. florida, P. sajor-cuju, P. pulmonarius, and P. spodoleucus) were idential, but P. cornucopiae differed from them in two bases out of 605 bases. However, p[hylogenetic analysis of the sequences by DNA-distance matrix and UPGMA methods showed that P. ostreatus NFFA2m1 and NFFA2m2, known as mutants of P. ostreatus NFFA2, belonged to anther group of Basidiomycotina, which is close to the genus Auricularia. The difference of the SSU rDNA sequences of P. cornucopiae from other Pleurotus species tested corresponds to the difference of mitochondrial plasmid type present in Pleurotus species as observed by Kim et al. (1993, Korean J. Microbiol. 31, 141-147).

• The Korean Journal of Mycology
• /
• v.15 no.3
• /
• pp.135-141
• /
• 1987

Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopiae protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.

• The Korean Journal of Mycology
• /
• v.15 no.1
• /
• pp.38-41
• /
• 1987

Neohaplonts were obtained to improve mushroom strain from dikaryon strains of Flammulina Velutips, Ganoderma lucidum, Lyophyllum ulmarium, Pleurotus cornucopiae and Pleurotus spodoleucus by protoplast reversion. The noehaplont frequency from reversion colonies of protoplasts was 4.47-47.7%. Four more types of morphological characteristics were detected from neohaplonts of protoplast reversion in L. ulmarium, P. cornucopiae and P. spodoleucus, but one or two types were neohaplonts in F.velutipes and G. lucidum. Growth rate of neohaplonts was slow compared with dikaryon strains.