Abstract
The research for incubation period, mycelial density, day required for primordial formation after inoculation(below DPI), number of valid stipes, individual weight and accumulation amounts of organic selenium for P. cornucopiae by treating 100, 200, 300, 400, 500(${\mu}g/50g$) of $Na_2SeO_3$ is following. Incubation periods of P. cornucopiae are 20~23 days per each low concentration treatment with $Na_2SeO_3$. Compared to the control which took 22 days of incubation period, it is reduced 1 or 2 days. Mycelial density of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $500{\mu}g/50g$ is very compact. DPI of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $400{\mu}g/50g$ was reduced 1 or 2days, but $500{\mu}g/50g$ was increased 1 day. Number of valid stipes of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $400{\mu}g/50g$ is between 19 and 20. It was increased 1 or 2, as compared to 18 of control, but $500{\mu}g/50g$ was reduced to 1. Individual weight of P. cornucopiae treated with $Na_2SeO_3$ between 100 and $400{\mu}g/50g$ was between 129 and 138g/850cc. It was increased 4.9~12.2% as compared to 123g/850cc of the control but $500{\mu}g/50g$ was 122g/50g. Accumulation amount of organic selenium for P. cornucopiae treated with $Na_2SeO_3$ between 100 and $500{\mu}g/50g$ was $2.73{\sim}8.19{\mu}g/g/dry$. It was increased 55~164 times as the concentration increased when compared to $0.05{\mu}g/g/dry$ of the control. In conclusion, incubation period, mycelial density, DPI, number of valid stipes, individual weight and accumulation amounts of organic selenium for P. cornucopiae by treating 100, ${\sim}400{\mu}g$ of $Na_2SeO_3$ was increased, but $500{\mu}g/50g$ was reduced. So more than $500{\mu}g/50g$ concentration treatments are required research.
100, 200, 300, 400, $500{\mu}g/50g$ 무기셀레늄($Na_2SeO_3$)의 배지처리에 의한 노랑느타리버섯 균사배양기간, 균사밀도, 초발이소요일수, 유효경수, 개체중, 유기셀레늄 전이량을 조사한 결과는 다음과 같다. 저농도 $Na_2SeO_3$의 처리에 의한 노랑느타리버섯 균사배양기간은 대조구 22일에 비하여 $100{\sim}400{\mu}g/50g$처리는 1~2일 단축되었고, $500{\mu}g/50g$처리는 1일 길어졌다. $Na_2SeO_3$의 처리에 의한 노랑느타리버섯 균사밀도는 $100{\sim}500{\mu}g/50g$처리는 아주 치밀하였다. $Na_2SeO_3$의 처리에 의한 노랑느타리버섯 초발이소요일수는 $100{\sim}400{\mu}g/50g$처리는 3~4일로 대조구 5일에 비해 1~2일 단축되었다. $500{\mu}g/50g$처리는 1일 길어졌다. $Na_2SeO_3$의 처리에 의한 노랑느타리버섯 유효경수는 $100{\sim}400{\mu}g/50g$처리는 19~20개로 대조구 18개에 비해 1~2개 증가되었다. $500{\mu}g/50g$처리는 17개로 대조구 18개에 비해 1개 적어졌다. $Na_2SeO_3$의 처리에 의한 노랑느타리버섯 수량(개체중)은 $100{\sim}400{\mu}g/50g$처리는 129~138g/850cc로 대조구 123g/850cc에 비해 4.9~12.2%까지 증수되었다. $500{\mu}g/50g$처리는 122g/850cc로 대조구 123g/850cc에 비해 수량이 감소되었다. $Na_2SeO_3$의 처리에 의한 노랑느타리버섯 축적된 유기셀레늄 량은 $100{\sim}500{\mu}g/50g$처리까지 $2.73{\sim}8.19{\mu}g/g/dry$로 농도가 높을수록 대조구 $0.05{\mu}g/g/dry$에 비해 55~164배까지 축적되었다. 결론적으로 노랑느타리버섯 균사배양기간, 균사밀도, 초발이소요일수, 유효경수, 개체중, 유기셀레늄 전이량은 $100{\sim}400{\mu}g/50g$까지는 촉진되었고 $500{\mu}g/50g$에서 억제되는 경향이 나타남에 따라 보다 고농도 처리시 그의 영향을 조사할 필요성을 시사한다.
(School of Biological Sciences and Technology and Biotechnology Research Institute, Chonnam National University)
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