• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.6C
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• pp.588-592
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• 2009

In this paper, we propose a novel method to improve the performance of the global soft decision which is based on the second-order conditional maximum a posteriori (CMAP). Conventional global soft decision scheme has an disadvantage in that the speech absence probability adjusted by a fixed-parameter was sensitive to the various noise environments. In proposed approach using the second-order CMAP, speech absence probability value is more flexible which exploit not only the current observation but also the speech activity decisions in the previous two frames. Experimental results show that the proposed improved global soft decision method based on second-order conditional MAP yields better results compared to the conventional global soft decision technique with the performance criteria of the ITU-T P. 862 perceptual evaluation of speech quality (PESQ).

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.72.2-73
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• 2003

We found a soybean (Glycine max) cultivar 561 that was strongly resistant to a virulent bacterial strain of a Pseudomonas spp. Further identification revealed that the Pseudomonas spp. was a strain of Pseudomonas aeruginosa. Furthermore we identified specific genes involved in the resistance of soybean 561 and analyzed the pattern of gene expression against the Pseudomonas infection using differential-display reverse transcription PCR (DDRT-PCR). More than 126 cDNA fragments representing mRNAs were induced within 48 hours of bacteria inoculation. Among them, 28 cDNA fragments were cloned and sequenced. Twelve differentially displayed clones with open reading frames had unknown functions. Sixteen selected cDNA clones were homologous to known genes in the other organisms. Some of the identified cDNAs were pathogenesis-related genes (PR genes) and PR-like genes. These cDNAs included a putative calmodulin-binding protein, an endo-1,3-1,4-b-D-glucanase, a b-1,3-endoglucanase, a b-1,3-exoglucanase, a phytochelatin synthetase-like gene, a thiol pretense, a cycloartenol synthase, and a putative receptor-like sorineithreonine protein kinase. Among them, we found that four genes were putative pathogenesis-related genes (PR) induced significantly by the p. aeruginosa infection. These included a calmodulin-binding protein gene, a b-1,3-endoglucanase gene, a receptor-like sorine/threonine protein kinase gene, and pS321 (unknown function). These results suggest that the differentially expressed genes may mediate the strong resistance of soybean 561 to Pseudomonas aeruoginosa.

• Journal of Broadcast Engineering
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• v.9 no.3
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• pp.225-235
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• 2004

In this paper, we propose a new stereoscopic video coding approach for heterogeneous consumer devices by exploiting the concept of spatio-temporal scalability. The proposed method uses MPEG-2 standard for coding the left or main sequence and an enhanced compatible coding scheme for predicting the P- and B-type of frames of the right or auxiliary sequence. The enhanced compatible coding scheme predicts matching block by interpolating both two forward and backward motion predicted macroblocks and disparity predicted macroblock. To provide flexible stereo video service, we define both a temporally scalable layer and a spatially scalable layer for each eye-view. The experimental results show the efficiency of proposed coding scheme by comparison with already known methods and the advantages of disparity estimation in terms of scalability overhead. According to the experimental results, we expect the proposed functionalities will play a key role in establishing highly flexible stereo video service for ubiquitous computing environment where devices and network connections are heterogeneous.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.10 s.352
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• pp.45-53
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• 2006

In this paper, we proposed a parallel hardware architecture for deblocking filter in K264/AVC. The deblocking filter has high efficiency in H.264/AVC, but it also has high computational complexity. For real time video processing, we chose a two 1-D parallel filter architecture, and tried to reduce memory access using dual-port SRAM. The proposed architecture has been described in Verilog-HDL and synthesized on Hynix 0.25um CMOS Cell Library using Synopsys Design Compiler. The hardware size was about 27.3K logic gates (without On-chip Memory) and the maximum operating frequency was 100Mhz. It consumes 258 clocks to process one macroblock, witch means it can process 47.8 HD1080P(1920pixel* 1080pixel) frames per second. It seems that it can be used for real time H.264/AVC encoding and decoding of various multimedia applications.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.8B
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• pp.1577-1585
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• 1999

In this paper, we propose detection methods for gradual scene changes such as dissolve, pan, and zoom. The proposal method to detect a dissolve region uses scene features based on spatial statistics of the image. The spatial statistics to define shot boundaries are derived from squared means within each local area. We also propose a method of the camera motion detection using four representative motion vectors in the background. Representative motion vectors are derived from macroblock motion vectors which are directly extracted from MPEG streams. To reduce the implementation time, we use DC sequences rather than fully decoded MPEG video. In addition, to detect the gradual scene change region precisely, we use all types of the MPEG frames(I, P, B frame). Simulation results show that the proposed detection methods perform better than existing methods.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1774-1783
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• 2015

Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp open reading frames, which encodes 148 amino acids (16.54 kDa) with a signal peptide of 18 amino acids, was sequenced. Further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. Recombinant yak milk lysozyme (rYML) was produced by Escherichia coli BL21 and Pichia pastoris X33. The highest lysozyme activity was detected for heterologous protein rYML5 (M = 1,864.24 U/mg, SD = 25.75) which was expressed in P. pastoris with expression vector $pPICZ{\alpha}A$ and it clearly inhibited growth of Staphylococcus aureus. Result of the YML gene expression using quantitative polymerase chain reaction showed that the YML gene was up-regulated to maximum at 30 day postpartum, that is, comparatively high YML can be found in initial milk production. The phylogenetic tree indicated that the amino acid sequence was similar to cow kidney lysozyme, which implied that the YML may have diverged from a different ancestor gene such as cow mammary glands. In our study, we suggest that YML be a new c-type lysozyme expressed in yak mammary glands that plays a role as host immunity.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.545-555
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• 2014

It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and $(NH_4)_2SO_4$ precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and $55^{\circ}C$ with 2,2'-azinobis-[ 3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and $60^{\circ}C$. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a $V_{max}$ value of 51.28 U/mg, and the Km and $V_{max}$ values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.8B
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• pp.751-759
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• 2009

Due to the advance of WLAN technology, the use of the multimedia service such as the video streaming service has been increased in the home network. However, we need to study the method which decreases the transmission delay and the frame loss rate to provide QoS of the video streaming service. Therefore, this paper proposes an active buffer management mechanism to guarantee QoS of the streaming service in IEEE 802.11e EDCA. The proposed protocol discards the frame in the HoL of the buffer based on the importance of each frame and the virtual transmission delay of frame newly arriving at the buffer. In the simulation results, the proposed algorithm not only decreases the frame loss probability of important I and P frames but also stabilizes the transmission delay. It may increase the QoS of video streaming services.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.6
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• pp.503-511
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• 2014

In this paper, a novel, real-time video stitching algorithm in an H.264/AVC compressed domain is proposed. This enables viewers to watch multiple video contents using a single device. The basic concept of this paper is that the server is asked to combine multiple streams into one bit-stream based in a compressed domain. In other words, this paper presents a new compressed domain combiner that works in boundary macroblocks of input videos with re-calculating intra prediction mode, intra prediction MVD, a re-allocation of the coefficient table, and border extension methods. The rest of the macroblocks of the input video data are achieved simply by copying them. Simulation experiments have demonstrated the possibility and effectiveness of the proposed algorithm by showing that it is able to generate more than 103 frames per second, stitching four 480p-sized images into each frame.

• BMB Reports
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• v.37 no.2
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• pp.206-212
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• 2004

The genes that are located within the odvp-6e/odv-e56 region of the Choristoneura fumiferana granulovirus (ChfuGV) were identified by sequencing the 11 kb BamHI restriction fragment on the ChfuGV genome. The global GC content that was calculated from the data obtained from this genomic region was 34.96%. The open-reading frames (ORFs), located within the odvp-6e/odv-e56 region, are presented and compared to the equivalent ORFs that are located at the same region in other GVs. This region is composed of 14 ORFs, including three ORFs that are unique to ChfuGV with no obvious homologues in other baculoviruses as well as eleven ORFs with homologues to granuloviral ORFs, such as granulin, CfORF2, pk-1, ie-1, odv-e18, p49, and odvp-6e/odv-e56. In this study, the conceptual products of seven major conserved ORFs (granulin, CfORF2, IE-1, ODV-E18, p49 and ODVP-6E/ODV-E56) were used in order to construct phylogenetic trees. Our results show that granuloviruses can be grouped in 2 distinct groups as follows: Group I; Choristoneura fumiferana granulovirus (ChfuGV), Cydia pomonella granulovirus (CpGV), Phthorimaea operculella granulovirus (PhopGV), and Adoxophyes orana granulovirus (AoGV). Group II; Xestia c-nigrum granulovirus (XcGV), Plutella xylostella granulovirus (PxGV), and Trichoplusia ni granulovirus (TnGV). The ChfuGV conserved proteins are most closely related to those of CpGV, PhopGV, and AoGV. Comparative studies, performed on gene arrangements within this region of genomes, demonstrated that three GVs from group I maintain similar gene arrangements.