• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.04a
• /
• pp.487-492
• /
• 1999

The purpose of this study to find the mixture of concrete for Top-Down method. Throughout test of elapsed time change and L-type flow, it was proposed basic performance and level of top-down method concrete. When change as to elapsed time is considered, so series of F10 added 10% S.P satisfied slump and demanded flow (60$\pm$5cm), and L-type slump, L-type flow satisfied liquidity, it can be considered basic mixture of designed actual frame later.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1993.04a
• /
• pp.61-61
• /
• 1993

사람의 혈액의 백혈구로부터 Polymerase Chain Reaction 방법으로 $\beta$$_2$-adrenergic receptor 유전자를 증폭하였다. 이 증폭된 유전자를 pbluescript KS(＋)에 cloning하였으며, 제한효소지도 작성과 부분적인 염기배열 확인으로 증폭된 유전자가 사람의 $\beta$$_2$-adrenergic receptor 유전자임을 확인하였다. $\beta$$_2$-adrenergic receptor 유전자를 N말단의 아미노산이 21개 제거되도록 결손시킨후 yeast의 pSec5의 Killer toxin signal sequence의 뒤에 in-frame으로 연결하였다. 현재 효모에서의 발현양상 및 발현된 단백질의 활성을 조사중이다.

• Proceedings of the IEEK Conference
• /
• 2001.06d
• /
• pp.109-112
• /
• 2001

This paper proposes a method to classify different video contents using features of digital video. Classified video types are the news, drama, show, sports, and talk program. Features, such as intra-coded macroblock number St motion vector in P-picture in MPEG domain are used. The frame difference of YCbCr is also employed as a measure of classification. We detect the occurrences of cuts in a video for a measure of classification. Finally, back-propagation neural-network of 3 layers is used to classify video contents.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.7
• /
• pp.960-964
• /
• 2016

The objective of this study was to investigate the effects of reducing dietary phosphorus (P) on the frame size, udder traits, blood parameters and nutrient digestibility coefficient in 8- to 10-month-old Holstein heifers. Forty-five heifers were divided into 15 blocks according to the mo of age and were randomly assigned one of three dietary treatments: 0.26% (low P [LP]), 0.36% (medium P [MP]), or 0.42% (high P [HP]) (dry matter basis). Samples were collected at the wk 1, 4, 8. The results show that low dietary P had no effect on body measurement. The blood P concentration decreased with decreasing dietary P (p<0.05), while the blood calcium content of LP was higher than that of the MP and HP groups (p<0.05), though still in the normal range. The serum contents of alkalinephosphatase, potassium, and magnesium were similar among the treatments. No differences were found in all nutrients' apparent digestibility coefficients with varied dietary P. However, with P diet decreased from HP to LP, the total fecal P and urine P concentration declined significantly, as did fecal water soluble P (p<0.05). In conclusion, reducing the dietary P from 0.42% to 0.26% did not negatively affect the heifers' growth performance but did significantly lessen manure P excretion into the environment.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.3 no.1
• /
• pp.57-62
• /
• 2001

A cDNA encoding the defensin homologue of a novel family member was isolated from the cDNA library of the firefly,Pyrocoelia rufa. Sequence analysis of the cDNA encoding the defensin homologue of P. rufa resulted that the 165 bp cDHA has an open reading frame of 55 amino acid residues. The deduced amino acid sequences of the defensin homologue gene from P. rufa showed identity to known mammalian defensins. Also 6 cystein residues in the P. rufa defensin homologue gene were conserved in the same position as those of known mammalian defensins. The result suggested that P. rufa defensin homologue is a novel member of the insect defensin family. Southern blot analysis suggests that there may be a single copy number of the P.rufa defensin homologue gene and their fat body-specific expression pattern at the transcriptional level was confirmed by Northern blot analysis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.8
• /
• pp.1057-1064
• /
• 2013

p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession GU144017) and its 3' noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3' noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.

• Journal of the Korean Society for Heat Treatment
• /
• v.26 no.5
• /
• pp.225-232
• /
• 2013

The influence of aging treatment, addition elements and rolling reduction ratio on the microstructure, mechanical, electrical and bendability properties of Cu-Ni-Si-P-x (x = Fe, Sn, Zn) alloys for connector material application was investigated. SEM/EDS analysis exhibited that Ni2-Si precipitates with a size of 20~100 nm were distributed in grains. Fe, Sn, Zn elemnets in Cu-Ni-Si-P alloy imporved the mechanical strength but it was not favor in increasing of electrical conductivity. As higher final rolling reduction ratio, the strength and electrical conductivity is increased after aging treatment, but it indicated excellent bendability. Especially, Cu-2Ni-0.4Si-0.5Sn-0.1Fe-0.03P alloy show the tensile strength value of 700MPa and the electrical conductivity was observed to reach a maximum of 40%IACS. It is optimal for lead frame and connector.

• Journal of Life Science
• /
• v.11 no.4
• /
• pp.385-391
• /
• 2001

Filamentous hemagglutinin (FHA) is considered as an essential immunogenic component for incorporation into acellular vaccines against Bordetella pertussis, the causative agent of whooping cough. Classically, antipertussis vaccination has employed an intramuscular route. An alternative approach to stimulate mucosal and systemic immune responses is oral immunization with recombinant live vaccine carrier strains of Salmonella typhimurium. An attenuated live Salmonella vaccine sgrain($\Delta$cya $\Delta$crp) expressing recombinant FHA(rFHA) was developed. Stable expressionof rFHA was achieved by the use of balanced-lethal vector-host system. which employs an asd deletion in the host chromosome to impose in obligate requirement for diaminopimelic acid. The chromosomal $\Delta$asd mutation was complemented by a plasmid vector possessing the asd$^{+}$ gene. A 3 kb DNA fragment encoding immuno dominant regionof FHA was subcloned in-frame downstream to the ATG translation initiation codon in the multicopy Asd$^{+}$ pYA3341 vector to create pYA3457. Salmonella vaccine harboring pYA3457 expressed approximately 105kDa rFHA protein. The 100% maintenance of [YA3457 in vaccine strain was confirmed by stability examinations. Additionally, a recombinant plasmid pYA3458 was constructed to overpress His(8X)-tagged rFHA in Essherichia coli. His-tagged rFHA was purified from the E. coli strain harboring pYA3458 using Ni$^{2+}$-NTA affinity purification system.>$^{2+}$-NTA affinity purification system.

• Journal of the Korean Society of Safety
• /
• v.32 no.6
• /
• pp.68-74
• /
• 2017

To predict the damage of Reinforced Concrete (RC) structures from mass explosion, Pressure-Impulse (P-I) curves representing the relationship between peak pressure and impulse based on damage criteria are essential. There are P-I curves developed by the U.S. DoD without detailed explanation regarding validation. In this study, full scale explosion tests were conducted measuring response of RC slab to modify and validate pre-existing P-I curves. Four same RC slabs were prepared, and placed at different distances, which are fixed to steel frame with concrete base. Scaled distances were selected to show different failure types using P-I curve based on Single Degree Of Freedom (SDOF) model. It was found that SDOF model can be used to evaluate and identify one-way RC slab damage with difference damage criteria.

• BMB Reports
• /
• v.40 no.3
• /
• pp.333-340
• /
• 2007

Gene encoding cyclodextrin glycosyltransferase (CGTase), from thermotolerant Paenibacillus sp. T16 isolated from hot spring area in northern Thailand, was cloned and expressed in E. coli (JM109). The nucleotide sequences of both wild type and transformed CGTases consisted of 2139 bp open reading frame, 713 deduced amino acids residues with difference of 4 amino acid residues. The recombinant cells required 24 h culture time and a neutral pH for culture medium to produce compatible amount of CGTase compared to 72 h culture time and pH 10 for wild type. The recombinant and wild-type CGTases were purified by starch adsorption and phenyl sepharose column chromatography and characterized in parallel. Both enzymes showed molecular weight of 77 kDa and similar optimum pHs and temperatures with recombinant enzyme showing broader range. There were some significant difference in pH, temperature stability and kinetic parameters. The presence of high starch concentration resulted in higher thermostability in recombinant enzyme than the wild type. The recombinant enzyme was more stable at higher temperature and lower pH, with lower $K_m$ for coupling reaction using cellobiose and cyclodextrins as substrates.