• Journal of The Korean Society of Integrative Medicine
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• v.10 no.4
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• pp.103-111
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• 2022

Purpose : In recent years, essential oils have been produced using natural extracts for various uses. Their functionality is currently being tested not only for cosmetics and perfumes but also for other categories of products. Therefore, this study verified their antibacterial effects on S. mutans, P. gingivalis, and L. gasseri which are the representative strains that cause oral diseases. Methods : Eighteen types of natural essential oils were made at a concentration of 50 % (v/v) using Tween 20, and their antibacterial effects were verified by applying S. mutans, P. gingivalis, and L. gasseri. The antibacterial effects were measured with the disc diffusion method. All the experiments were repeated three times, and the mean value of three measurement values for each variable was used for data analysis. A one-way analysis of variance was conducted using these mean values. Results : Of the eighteen types of essential oils tested, sixteen types showed antibacterial effects on S. mutans, and sixteen and fifteen types exhibited antibacterial effects on P. gingivalis and L. gasseri respectively. The types of essential oils with high-level antibacterial activities were geranium, may chang, and bergamot for S. mutans, lemongrass, bergamot, and eucalyptus for P. gingivalis, and lemongrass, machan, and geranium for L. gasseri in order of antibacterial effect. This result was statistically significant (p<.001). In addition, in the case of mandarin oil, it was found that there was no antibacterial activity in all three strains. Conclusion : This study proved the antibacterial activities of essential oils, which are used for various purposes in daily life, against dental caries and periodontal diseases. The study results will likely be applied to different prevention programs for oral health and broadly used to develop products such as oral care items and dentifrices.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.5
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• pp.455-462
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• 2000

The objective of this study was to examine the physicochemical characteristics of coagulation reaction between loess and red tide organisms (RTO) and its feasibility, in developing a technology for the removal of RTO bloom in coastal sea. The physicochemical characteristics of loess were examined for a particle size distribution, surface characteristics by scanning electron microscope, zeta potential, and alkalinity and pH variations in sea water. Two kinds of RTO that were used in this study, Cylindrothen closterium and Skeietonema costatum, were sampled in Masan bay and were cultured in laboratory. Coagulation experiments were conducted using various concentrations of loess, RTO, and a jar tester. The supernatant and RTO culture solution were analyzed for pH, alkalinity, RTO cell number. A negative zeta potential of loess increased with increasing pH at $10^(-3)M$ NaCl solution and had -71.3 mV at pH 9.36. Loess had a positive zeta potential of +1,8 mV at pH 1.98, which resulted in a characteristic of material having an amphoteric surface charge. In NaCl and $CaCl_2$, solutions, loess had a decreasing negative zeta potential with increasing $Na^+\;and\;Ca^(+2)$ ion concentration and then didn't result in a charge reversal due to not occurring specific adsorption for $Na^+$ ion while resulted in a charge reversal due to occurring specific adsorption for $Ca^(+2)$ ion. In sea water, loess and RTO showed the similar zeta potential values of -112,1 and -9.2 mV, respectively and sea sand powder showed the highest zeta potential value of -25.7 mV in the clays. EDLs (electrical double-layers) of loess and RTO were extremely compressed due to high concentration of salts included in sea water, As a result, there didn't almost exist EDL repulsive force between loess and RTO approaching each other and then LVDW (London-yan der Waals) attractive force was always larger than EDL repulsive force to easily form a floe. Removal rates of RTO exponentially increased with increasing a loess concentration. The removal rates steeply increased until $800 mg/l$ of loess, and reached $100{\%}$ at 6,400 mg/l of loess. Removal rates of RTO exponentially increased with increasing a G-value. This indicated that mixing (i.e., collision among particles) was very important for a coagulation reaction. Loess showed the highest RTO removal rates in the clays.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.340-341
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• 2012

In this work, we investigated the effects of temperature stress on flatband voltage (VFB) shifts of HfO2-SiO2 double gate dielectrics devices. Fig. 1 shows a high frequency C-V of the device when a positive bias for 10 min and a subsequent negative bias for 10 min were applied at room temperature (300 K). Fig. 2 shows the corresponding plot when the same positive and negative biases were applied at a higher temperature (473.15 K). These measurements are based on the BTS (bias temperature stress) about mobile charge in the gate oxides. These results indicate that the positive bias stress makes no difference, whereas the negative bias stress produces a significant difference; that is, the VFB value increased from ${\Delta}0.51$ V (300 K, Fig. 1) to ${\Delta}14.45$ V (473.15 K, Fig. 2). To explain these differences, we propose a mechanism on the basis of oxygen vacancy in HfO2. It is well-known that the oxygen vacancy in the p-type MOS-Cap is located within 1 eV below the bottom of the HfO2 conduction band (Fig. 3). In addition, this oxygen vacancy can easily trap the electron. When heated at 473.15 K, the electron is excited to a higher energy level from the original level (Fig. 4). As a result, the electron has sufficient energy to readily cross over the oxide barrier. The probability of trap about oxygen vacancy becomes very higher at 473.15 K, and therefore the VFB shift value becomes considerably larger.

• Korean Journal of Environment and Ecology
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• v.14 no.1
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• pp.46-56
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• 2000

Vegetation structure of $Hwangs\v{o}ng$ Park in $Ky\v{o}ugju$ was investigated to study ecological value and to suggest a desirable management proposal. To do this, twenty plots were set up and surveyed. The $Hwangs\v{o}ng$ Park forest was classified into five plant communities by the DCA technique, which were two types of Pinus densiflora communities, Quercus spp.-P. densiflora, Robinia pseudoacacia and Q. mongolica community. In the diversity analysis, 29 woody species were observed, but species composition was simple. No. of average species is $5.4\pm2.9$ per a plot($100m^2$), and Pinus densiflora is a dominant species in DBH $27\sim47cm$ class. From the results of tree ring and growth analysis, it was found out that pinus densiflora community of $Hwangs\v{o}ng$ Park has a historical and ecological value, but recently the growth rate was decreasing. The management proposal was suggested upon to the above results.

• Journal of Dairy Science and Biotechnology
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• v.34 no.2
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• pp.117-135
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• 2016

The present study was performed to develop a functional raw food material from hydrolyzed whey protein powder (23%-GNANA) medication containing sialic acid as a marker compound that is naturally occurring at 7% concentration in GMP (glycomacropeptide). GMP is used worldwide in foodstuffs for babies and infants and is obtained from the milk protein as safe food. While the purpose of our detailed evaluation was aimed to assess preliminary NOAEL values for and above 2,000 mg/kg/day, a clinical dose allowance for 23%-GNANA (as per characteristic of a functional health product, a highly refined test substance of 23% (v/v) sialic acid combined in GMP), at the same time we also wanted to assess the safety of GMP hydrolyzate lacking sialic acid but with identical properties as GMP. Animal safety evaluation was conducted using 23%-GNANA as the test substance, produced from hydrolyzed whey protein powder (product name: HELICOBACTROL-23; provided by Medinutrol Inc. [Korea]; composed of 23% sialic acid and GMP protein) after isolating the sialic acid using enzymes approved as food additives, with GMP as a raw material, and subsequently increasing the content of xx up to 23% through 80% (v/v) ethanol soaking and concentrating, in accordance with GLP Guideline. The animal safety evaluation mentioned above was made on the basis of toxicity in SPF Sprague-Dawley female and male rats dosed with 10 mL of the test substance diluted to 0, 1,250, 2,500, and 5,000 mg/kg directly into their stomachs for 90 d. This was determined in terms of the general symptoms and animal viability, weight and amount of feed intake, eye examination, uracrasia tests, hematological and blood biochemical disorder tests, blood coagulation test, abnormal intestine weight, abnormalities during postmortem and histopathological examinations. Statistical significance was set at P<0.05. Based on the toxicity determination, a certain minor effect associated with the test substance was observed in male rats with no major effects of the tested substance, in comparison with the control group dosed with sterilized water. Nevertheless, the NOAEL value, evaluated as per toxicity criteria, was verified as 5,000 mg/kg/day (P<0.05). Similarly, for female rats, a certain minor effect associated with the test substance was observed in 5,000 mg/kg/day dosed group, with no major effect, yet the NOAEL value (as assessed as per toxicity criteria) was determined to be 5,000 mg/kg/day (P<0.05), which was the same as for male rats. Accordingly, the NOAEL values of the test substances for all female and male rats were finally verified as 5,000 mg/kg/day (P<0.05). In conclusion, it was determined that the 23%-GNANA test substance exceeds 2,000 mg/kg/day, the clinical allowance characteristic for functional health food, and was finally evaluated to cause no safety concerns when used as a raw material in functional health food production, which was the ultimate goal of the present study.

• The Korean Journal of Ecology
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• v.13 no.4
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• pp.297-309
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• 1990

The distribution of aquatic macrophytes in TanChon basin, a stream of the Han River, were investigated in terms of environmental gradient from June 1989 to March 1990. In the basin, 12 species of aquatic macrophytes were listed and four communities of Potamogeton crispus community. $P. octandrus$ community, $Hydrilla verticillata$ community and $Vallisneria$ asiatica community were recongized by character species. $P. crispus, P. octandrus$ and $V. asiatica$ were found in rapids while $H. verticillata , Ceratophyllum demersum$ and $Trapa japonica$ were done in pools. The depth of sediment $TanCh\u{o}n$ was showed as a exponential function of water velocity, Bd=exp (-K Wv). The values of Biochemical Oxygen Demand(BOD), Chemical Oxygen Demand(COD) and Suspendid Solid(SS) were recorded as range of 3.2~121.0mg/1, 4.2~54.5mg/1 and 4.1~114.0mg/1, respectively. And the linear positive correlation between BOD(X) and COD(Y) were expressed as Y=3.904+0.4308 X with $R^2$=0.9808 and also the correlation between BOD value(X) and SS value(Y) were done as Y=5.333+0.9606X with $R^2$=0.9700. In two dimensional analysis of BOD and water velocity, their clusters were showed similar types matching to communities classified by character species. However, no aquatic macrophyes were found at the site with BOD$\geq$50mg/l or DO$\leq$0.2mg/l.

• Investigative Magnetic Resonance Imaging
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• v.18 no.1
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• pp.25-33
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• 2014

Purpose : To investigate whether quantitative parameters derived from Diffusion-weighted magnetic resonance imaging (DW-MRI) correlate with those of Dynamic contrast-enhanced MRI (DCE-MRI). Materials and Methods: Thirteen patients with pathologically or clinically proven bony metastasis who had undergone MRI prior to treatment were included. The voxel size was $1.367{\times}1.367{\times}5mm$. A dominant tumor was selected and the apparent diffusion coefficient (ADC) value and DCE-MRI parameters were obtained by matching voxels. DCE-MRI data were analyzed yielding estimates of $K^{trans}$ (volume transfer constant) and $v_e$. (extravascular extracellular volume fraction). Statistical analysis of ADC, $K^{trans}$, and $v_e$ value was conducted using Pearson correlation analyses. Results: Fifteen lesions in pelvic bones were evaluated. Of these, 11 showed a statistically significant correlation (P<0.05) between ADC and $K^{trans}$. The ADC and $K^{trans}$ were inversely related in 7 lesions and positively related in 4 lesions. This did not depend on the primary cancer or site of metastasis. The ADC and $v_e$ of 9 lesions correlated significantly. Of these, 4 lesions were inversely related and 5 lesions were positively related. Conclusion: Unlike our theoretic hypothesis, there was no consistent correlation between ADC values and $K^{trans}$ or between ADC values and $v_e$ in metastatic bone tumors.

• Economic and Environmental Geology
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• v.45 no.1
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• pp.23-30
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• 2012

Major ions and nitrogen isotopic analyses were performed to determine the geochemical characters and to identify the source of nitrate of the shallow groundwater around agricultural field in the Youngdong area. The pH value of groundwater ranges from 60. to 8.2 (pH 7.2, mean). The average of EC, Eh and DO is 369 ${\mu}S/cm$ (70~729 ${\mu}S/cm$), 165.6 mV (29~383.2 mV), 4.3 mg/L (1.8~8.0 mg/L) respectively. The ion concentraion of groundwater was in the order of $Ca^{2+}$>$Na^{2+}$>$Mg^{2+}$>$K^{2+}$ and ${HCO_3}^-$>${NO_3}^-$>${SO_4}^{2-}$>$Cl^-$>$F^-$. Most of groundwater is Ca-$HCO_3$ type. The groundwater was affected by water-rock interaction in the shallow depth. Some groundwater is Ca-Cl or Na-$HCO_3$ (2.5%) type that was due to agricultural activities. The $NO_3$_N concetration of grondwater range from 10.2 mg/l to 26.9 mg/l, which show that this area is under nitrate pollution. ${\delta}^{15}N-NO_3$ value of the groundwater is the origins of are a combination of animal wastes and man-made fertilizers.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.403-410
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• 1996

Variations of temperature and physicochemical environment during composting of a mixtures(2:1, v/v) of cattle manure and rice hulls(CMR) in two different composting methods, static windrow(SW) and aerated static pile system(ASPS), were monitored for evaluating the efficient composting system in greenhouse. The pH of composting materials increased to around 8.9 initially, then decreased and stabilized slowly to the neutral value. Composting materials in ASPS showed a rapid stabilization in pH value from the 4th week comparing to the speed in SW. Thermophilic stage for ASPS Lasted at 3 week whereas 6 weeks for WS. Required time to get thermophilic zone in compost was shorter in ASPS than in WS. Reduction rate in total carbon(T-C) was higher in ASPS than in WS. Organic matter was reduced more rapidly in ASPS than in SW showing 9 percent difference after the 6th week. Total nitrogen(T-N) increased while composting process, showing 9 percent after 6th week in WS and 1.8 percent after 7th week in ASPS. C/N ratio was stabilized after 6th week showing 17 and 21 level in WS and ASPS each. Quantity of ash and mineral content increased during composting in both system, showing higher content in ASPS. Composting process by intermittent, aerated static pile system in greenhouse had a significant effect on the reduction of required period for composting.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.272-279
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• 2003

This study was performed to develop a more rapid and simple nutrient diagnosis method of plants than the conventional leaf analysis method. Cucumber (Cucumis sativus L. cv. jangil banbaek) was planted in the mixed media produced by mixing perlite and rock wool at 1:1 (v/v) ratio. The Yamazakki nutrient solution for cucumber was supplied to the media using micro-drip irrigation system. Experimental plots were consisted of no fertilization, deficient fertilization, adequate fertilization, and surplus fertilization for N, P and K. Specific color difference sensor value (SCDSV) measured by chlorophyll meter was closely related to total-N concentration in leaves measured by the conventional method. Nitrate, $PO_4$ and K concentration in petiole sap measured by test strips showed a significant relationship with total-N, P and K concentration in leaves. Linear regression equations between $NO_3$, $PO_4$ and K concentration in petiole sap and total-N, P and K concentration in the leaves were prepared. Optimum levels of $NO_{3}$, $PO_{4}$ and K in petiole sap were obtained by plugging the optimum concentrations of total-N, P and K in the leaves by other researchers into the equations. In conclusion, the SCDSV measured by chlorophyll meter and the concentration of $NO_3$, P and K in petiole sap measured by the test strips would be suitable for rapid estimation of plant nutrient status.