• Journal of Chest Surgery
• /
• v.39 no.1 s.258
• /
• pp.1-11
• /
• 2006

Background: CD44 is a glycoprotein on the cell surface which is involved in the cell-to-cell and cell-to-matrix interaction. The standard form, CD44s and multiple isoforms are determined by alternative splicing of 10 exons. Recent studies have suggested that CD44 may help invasion and metastasis of various epithelial tumors as well as activation of Iymphocytes and monocytes. The expression pattern of CD44 can be different according to tumor types. The author studied the expression pattern of CD44s and one of its variants, CD44v6 in non-small cell lung carcinomas (NSCLC) to find their implications on clinicopathologic aspects, including the survival of the patients. Material and Method: A total of 89 primary NSCLSs (48 squamous cell carcinomas, 33 adenocarcinomas, and 8 undifferentiated large cell carcinomas) were retrieved during the years between 1985 to 1994. The immunohisto chemistry was done by using monoclonal antibodies and the CD44 expression for angiogenesis was evaluated by counting the number of tumor microvessels. Result: Seventy-one (79.8$\%$) and 64 (71 .9$\%$) among 89 NSCLSs revealed the expression of CD44s and CD44v6, respectively. The expression of CD44s was well correlated with that of CD44v6 (r=0.710, p < 0.0001). The expression of CD44s and CD44v6 was associated with the histopathologic type of the NSCLCs, and squamous cell carcinoma was the type that showed the highest expression of CD44s and CD44v6 (p < 0.0001). Microvessel count was the highest in adenocarcinomas (113.6$\pm$69.7 on 200-fold magnification and 54.8$\pm$41.1 on 400-fold magnification) and correlated with the tumor size of TNM system (r=0.217, p=0.043) and CD44s expression (r=0.218, p=0.040). In adenocarcinoma, the patients with higher CD44s expression survived shorter than those with lower CD44s expression (p=0.0194) but there was no statistical significance on multivariate analysis(p=0.3298). Conclusion: The expression of both CD44s and CD44v6 may be associated with the squamous differentiation in non-small cell lung carcinomas. The relationship of CD44s expression with micro-vessel density of the tumor suggests an involvement of CD44s in tumor angiogenesis, which in turn would help tumor growth.

• Korean Journal of Environment and Ecology
• /
• v.30 no.1
• /
• pp.39-47
• /
• 2016

Recently, it has been reported that morphologically and genetically different two Siberian roe deer (Capreolus pygargus, Pallas, 1771), in Mainland and in Jeju island. Until now, this roe deer has not been clearly taxonomy of Siberian roe deer in Korean peninsula. So, the aim of this study was to compare the skull morphometrics of mainland and Jeju roe deer to obtain more information on the species status of C. pygargus and study the distribution of roe deer subspecies in this region. A total of 50 skulls of adult roe deer was compared and analyzed. The maximum skull length, condylo-basal length, basilar length, maximum skull width, cheek-bone width, interorbital width, rostrum length, length of upper tooth row from the Siberian roe deer in Jeju island were significantly smaller than those of the deer in mainland. Length of the skull was the principal factor of interpopulational variability in roe deer. Variation was not distributed along latitude, longitude, or elevation, but those factors did influence the variation through effects on primary production, which in turn caused isolated differences in skull morphology apparently related to types of forage selected by roe deer in various geographic areas. According to the analysis results of the skull characters, Mainland roe deer was more similar to C. p. tianschanicus which is a subspecies of Siberian roe deer. In addition, Jeju roe deer showed distinct differences compared to European roe deer, 3 subspecies of Siberian roe deer, so it appeared that they could be a unique native species inhabited only in Jeju Island. Because Siberian roe deer in Jeju Island can be classified at subspecies level in this study.

• Korean Journal of Environment and Ecology
• /
• v.28 no.1
• /
• pp.33-44
• /
• 2014

The purpose of this study was to provide basic information and investigate a vegetation structure around Tohamsan (Mt.) Wetland. Actual vegetation map was made on the basis of watershed around that. Vegetation structure survey was carried out for 8 representative communities of actual vegetation which were Pinus densifrora community, Quercus variabilis community, Cornus controversa community, Q. serrata community, Q. mongolica-Q. serrata community, Salix koreensis community, Q. mongolica community, Q. variabilis-Q. mongolica-Q. serrata community. Tohamsan (Mt.) Wetland is located on 490m above sea level and the area of watershed was $236,272m^2$. Vegetation type were divided into 16 types, and the ratio of Q. mongolica community was 33.1% ($78,209.2m^2$). In order to turn out the structure of 8 representative communties, 32 plots were set up and unit area of plot was $100m^2$. The estimated age of forest is 30~50-years-old, and in the resutls of soil analysis, acidity was pH 4.89 and organic matter was 4.46%.

• Journal of Korean Academy of Nursing
• /
• v.3 no.1
• /
• pp.85-95
• /
• 1972

For the purpose to clarify the effects of nursing, intervention with comfort measures which promote rest, exercise and sleep on the patient′s rehabilitation, this study was carried out on 119 postoperative patients at St. Mary′s Hospital, the National Medical Center and Seoul Red Cross Hospital during the 9 months period from March 1971 to November 1971. In this study one experimental nursing approach was utilized; an emphasis on interpersonal techniques along with physical care-comfort measures. A daily evening care including support and instruction was given to facilitate interaction of nursing to the experimental group by the investigator. For the control group, routine hospital nursing care was performed. The nursing observation was followed for 4 days postoperatively and recorded in check list. The results of the findings were as follows. 1. 3.5% of control group and 32.3% of experimental group got out of bed within 24 hours postoperatively. 38.6% of control group got out of bed within 72 hours postoperatively where only 16.1% of the experimental group did (x$^2$= 19.865, p<0.005). Interaction in nursing is, in turn, significantly more effective than the usual routine care in improving rate of healing. 2. The irritations and tension that may interfere patient′s sleep and rest at night can be reduced to a minimum if nursing environment is better controlled with planned nursing care for individual patient. Various treatments which tend to give patient discomfort may preferably be performed before 6 p.m. if not absolutely indicated. 3. During 4 days of observation the patients without administration of sedatives and analgesics postoperatively were 25.9% in the experimental group where as 10.5% in the control group. The frequency of administration of sedatives and analgesics in average was 1.4 in the experimental group, and 2.0 in the control group. This indicates that not all postoperative discomforts expressed by the patients should be regarded as incision pain, and those discomforts could be relieved to a certain extent by nursing interventions effectively. 4. There were significant differences between the responses to nursing care given in the experimental group and 33% of the control group in average through 4 days of observation responded "good". 3.6% of the experimental group and 17. 1% of the control group responded "poor" in this study. It was recommended that the study be replicated in a more defined and controlled manner. Some alternative areas for investigation were suggested.

• Asian-Australasian Journal of Animal Sciences
• /
• v.23 no.1
• /
• pp.116-121
• /
• 2010

Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.12 no.1
• /
• pp.41-45
• /
• 1983

The method of intestinal Ca absorption in rats was studied by injecting the test solution of $^{45}Ca$ into ligated ileal loops (an in situ closed gut sement technique). The amount of Ca absorbed was measured in regard to the amount of soluble $^{40}Ca$ and $^{45}Ca$ which disappeared from the ligated ileal loops for 30min, and appeared portal blood for 15 min after the injection. The 15~20cm ligated segment of mid ileum and the test solution containing $0.2{\sim}0.5{\mu}Ci$ $5{\mu}g$ Ca/0.5ml, pH 7.0, $37^{\circ}C$ are appropriate for this in situ closed gut segment technique which turn out to be highly available for study of Ca absorption. The rats fed casein diet absorbed more Ca by 2~5 times than the rats fed egg albumin diet.

• Journal of Chest Surgery
• /
• v.44 no.6
• /
• pp.406-412
• /
• 2011

Background: Development of thoracic aortic aneurysms and aortic dissections (TAAD) is attributed to unbearable wall tension superimposed on defective aortic wall integrity and impaired aortic repair mechanisms. Central to this repair mechanisms are well-balanced and adequately functional cellular components of the aortic wall, including endothelial cells, smooth muscle cells (SMCs), inflammatory cells, and adventitial fibroblasts. Adventitial fibroblasts naturally produce aortic extracellular matrix (ECM), and, when aortic wall is injured, they can be transformed into SMCs, which in turn are involved in aortic remodeling. We postulated the hypothesis that adventitial fibroblasts in patients with TAAD may have defects in ECM production and SMC transformation. Materials and Methods: Adventitial fibroblasts were procured from the adventitial layer of fresh aortic tissues of patients with TAAD (Group I) and of multi-organ donors (Group II), and 4-passage cell culture was performed prior to the experiment. To assess ECM production, cells were treated with TNF-${\alpha}$ (50 pM) and the expression of MMP-2/MMP-3 was analyzed using western blot technique. To assess SMC transformation capacity, cells were treated with TGF-${\beta}1$ and expression of SM ${\alpha}$-actin, SM-MHC, Ki-67 and SM calponin was evaluated using western blot technique. Fibroblasts were then treated with TGF-${\beta}1$ (10 pM) for up to 10 days with TGF-${\beta}1$ supplementation every 2 days, and the proportion of transformed SMC in the cell line was measured using immunofluorescence assay for fibroblast surface antigen every 2 days. Results: MMP-3 expression was significantly lower in group I than in group II. TGF-${\beta}1$-stimulated adventitial fibroblasts in group I expressed less SM ${\alpha}$-actin, SM-MHC, and Ki-67 than in group II. SM-calponin expression was not different between the two groups. Presence of fibroblast was observed on immunofluorescence assay after more than 6 days of TGF-${\beta}1$ treatment in group I, while most fibroblasts were transformed to SMC within 4 days in group II. Conclusion: ECM production and SMC transformation are compromised in adventitial fibroblasts from patients with TAAD. This result suggests that functional restoration of adventitial fibroblasts could well be a novel approach for the prevention and treatment of TAAD.

• The Korean Journal of Quaternary Research
• /
• v.17 no.2
• /
• pp.17-23
• /
• 2003

In Korea terrestrial fluvial sequences can be used as pedological and sedimentological markers indicating a millenium-scale environmental and climatic changes imprinted in fluvial sub-environments, which in turn are represented by the cyclicity of fluvial sands, backswamp organic muds, and flooding muds intercalations of frostcracked or dessicated brown paleosols. Post LGM and Holocene fluvial and alluvial sedimentary sequences of Korea are formed in such landscapes of coastal, floodplain, backswamp and hillslope areas. Among them, the most outstanding depositional sequences are fluvial gravels, sands and organic mud deposits in coastal, fluvial, or alluvial wetlands. The aim of this study is to explain the sedimentary sequences and palynofloral zones since the last 15,000years, on the basis of organic muds layers intercalated in fluvial sand deposits. Jangheung-ri site of Nam river, Soro-ri site of Miho river, Youngsan rivermouth site in Muan, Oksan-ri site of Hampyeong and Sanggap-ri site of Gochang are illustrated to interpret their sedimentary facies, radiocarbon datings, and palynofloral zonation. Up to the Middle to Late Last Glacial(up to 30-35Ka), old river-bed, flooding, and backswamp sequences contain such arboreal pollens as Pinus, Abies, and Picea, and rich in non-arboreal pollens like Cyperaceae, Gramineae, Ranunculaceae, and Compositae. During the LGM and post-LGM periods until Younger Dryas, vegetation has changes from the sub-alpine conifer forest(up to about 17-11Ka), through the conifer and broad-leaved deciduous forest, or mixed forest (formed during 16,680-13,010yrB.P), to the deciduous and broad-leaved forest (older than 9,500yrB.P). In the Earliest Holocene flooding deposits, fragments of plant roots are abundant and subjected to intensive pedogenic processes. During Holocene, three arboreal pollen zones are identified in the ascending order of strata； Pinus-Colyus zone(mixed conifer and deciduous broad-leaved forest, about up to 10Ka), Alnus-Quercus forest (the cool temperate deciduous broad-leaved forest, about 10Ka-2Ka), and Pinus forest (the conifer forest, about after 2Ka), as examplified in Soro-ri site of Cheonwon county. The palynological zonations of Soro-ri, Oksan-ri, Sanggap-ri, Youngsan estuary, and Gimhae fluvial plain have been recognized as a provisional correlation tool, and zonations based on fluvial backswamp and flooding deposits shows a similar result with those of previous researchers.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.1
• /
• pp.118-126
• /
• 2011

Ester-linked fatty acid methyl ester (EL-FAME) profiles were used to describe differences in soil microbial communities influenced by conventional farming system (CFS), and organic farming system (OFS) in controlled horticultural land. Soil physicochemical properties and soil microbial communities were determined in the experimental fields. Higher organic matter content in OFS reduced soil bulk density which in turn increased the soil porosity. Generally, soil chemical properties in OFS were higher than those of CFS, but EC value in OFS was significantly lower than that of CFS. With the exception of Fe content, other macronutrient contents and pH in both farming system decreased with the soil depth. Soil microbial biomass of OFS was approximately 1.3 times in topsoil and 1.8 times in subsoil higher than those of CFS. Lower ratios of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ were found in the CFS soils than the OFS soils, indicating that microbial stress decreased. The ratio of MUFA to SFA was higher in OFS due to organic input to the soil. In principal components analysis (PCA), the first variable accounted for 54.3%, while the second for 27.3%, respectively. The PC1 of the PCA separated the samples from CFS and OFS, while the PC2 of the PCA separated the samples from topsoil and subsoil. EL-FAMEs with the positive eigenvector coefficients for PC1 were cy17: 0 to $16:1{\omega}7c$ ratio, cy19:0 to $18:1{\omega}7c$ ratio, soil pH, soil organic matter, and soil $NO_3$-N content. Our findings suggest that the shifting cy19:0 to $18:1{\omega}7c$ ratio should be considered as potential factors responsible for the clear microbial community differentiation observed between different cultivation systems and soil depth in controlled horticultural land.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.16 no.1
• /
• pp.26-37
• /
• 2017

The study is to develop safety performance functions(SPFs) for urban intersections using random effects Tobit regression model and to analyze correlations between crashes and factors. Also fixed effects Tobit regression model was estimated to compare and analyze model validation with random effects model. As a result, AADT, speed limits, number of lanes, land usage, exclusive right turn lanes and front traffic signal were found to be significant. For comparing statistical significance between random and fixed effects model, random effects Tobit regression model of total crash rate could be better statistical significance with $R^2_p$ : 0.418, log-likelihood at convergence: -3210.103, ${\rho}^2$: 0.056, MAD: 19.533, MAPE: 75.725, RMSE: 26.886 comparing with $R^2_p$ : 0.298, log-likelihood at convergence: -3276.138, ${\rho}^2$: 0.037, MAD: 20.725, MAPE: 82.473, RMSE: 27.267 for the fixed model. Also random effects Tobit regression model of injury crash rate has similar results of model statistical significant with random effects Tobit regression model.