• Title/Summary/Keyword: P-E

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A Comparison of Natural (D-α-tocopherol) and Synthetic (DL-α-tocopherol Acetate) Vitamin E Supplementation on the Growth Performance, Meat Quality and Oxidative Status of Broilers

  • Cheng, K.;Niu, Y.;Zheng, X.C.;Zhang, H.;Chen, Y.P.;Zhang, M.;Huang, X.X.;Zhang, L.L.;Zhou, Y.M.;Wang, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.5
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    • pp.681-688
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    • 2016
  • The present study was conducted to compare the supplementation of natural (D-${\alpha}$-tocopherol) and synthetic (DL-${\alpha}$-tocopherol acetate) vitamin E on the growth performance, meat quality, muscular antioxidant capacity and genes expression related to oxidative status of broilers. A total of 144 1 day-old Arbor Acres broiler chicks were randomly allocated into 3 groups with 6 replicates of 8 birds each. Birds were given a basal diet (control group), and basal diet supplemented with either 20 IU D-${\alpha}$-tocopherol or DL-${\alpha}$-tocopherol acetate for 42 days, respectively. The results indicated that treatments did not alter growth performance of broilers (p>0.05). Compared with the control group, concentration of ${\alpha}$-tocopherol in the breast muscle was increased by the supplementation of vitamin E (p<0.05). In the thigh, ${\alpha}$-tocopherol content was also enhanced by vitamin E inclusion, and this effect was more pronounced in the natural vitamin E group (p<0.05). Vitamin E supplementation increased the redness of breast (p<0.05). In the contrast, the inclusion of synthetic vitamin E decreased lightness of thigh (p<0.05). Dietary vitamin E inclusion reduced drip loss at 24 h of thigh muscle (p<0.05), and this effect was maintained for drip loss at 48 h in the natural vitamin E group (p<0.05). Broilers given diet supplemented with vitamin E showed decreased malondialdehyde (MDA) content in the breast (p<0.05). Additionally, natural rather than synthetic vitamin E reduced MDA accumulation in the thigh (p<0.05). Neither natural nor synthetic vitamin E supplementation altered muscular mRNA abundance of genes related to oxidative stress (p>0.05). It was concluded that vitamin E supplementation, especially the natural vitamin E, can enhance the retention of muscular ${\alpha}$-tocopherol, improve meat quality and muscular antioxidant capacity of broilers.

Mapping of Gene Encoding Phospho-$\beta$-galactosidase from Lactobacillus casei and its Expression in Escherichea coli (Lactobacillus casei 의 Phospho-$\beta$-galactosidase 유전자의 지도작성과 Escherichia coli 내에서의 발현)

  • 박정희;문경희;민경희
    • Korean Journal of Microbiology
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    • v.30 no.6
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    • pp.539-545
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    • 1992
  • Recombinant plasmid pPLac15 determined both phosphoenolpyruvate-dependent phosphotransferase uptake of lactose and phospho-$\beta$-galactosidase (Moon et al., 1989). A restriction mapping of the pPLac15 was compiled with several restriction enzymes and a seriese of sub clones into pUC18 was constructed. From an analysis of the proteins produced by Escherichia coli cells of transformants containing each of the recombinant subclone plasmids, it was found that the gene for phospho-$\beta$-galactosidase in pUCI8 was expressed about 1.8-folds in E. coli.

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Preparation and Effect of Eudragit E100 Microcapsules Containing Grapefruit Seed Extract on Kimchi (자몽씨 추출물을 함유한 Eudragit E100 미세캡슐의 제조 및 김치에 대한 영향)

  • 김한수;정성기;조성환;구재관;이승철
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1239-1244
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    • 2003
  • Microcapsules were prepared by coacervation method using acetone/liquid paraffin system to control the ripening of kimchi. Eudragit E100, which was soluble at below pH 5.0 in aqueous solution, was used to make microcapsules to be sensitive to acidity of kimchi. The microcapsules with Eudragit E100 containing grapefruit seed extract (GFSE) showed the highest yield of 92.13%, the size of microcapsules was decreasing as increasing the amount of aluminium stearate, a dispersing agent. Morphology of the microcapsules determined by scanning electron microscopy showed spherical forms. GFSE, encapsulated antimicrobial agents, was quickly released at acidic buffer (pH 4,5,6) within 1 storage day. However, 70% of encapsulated GFSE in Eudragit E100 microcapsules was continuously released at pH 7 till 3 days, and it was sustained till 9 days. Characteristics of kimchi containing microcapsules of GFSE were analysed with ripening period. Decease of pH in kimchi was retarded with the added GFSE microcapsules till 2 days of fermentation, but GFSE did not affect pH in kimchi after 3 days. Total numbers of microorganisms and lactic acid microorganisms in kimchi were decreased with increasing the amount of the added GFSE microcapsules, however, the effect of controlled released GFSE from pH sensitive Eudragit E100 microcapsules was hard to detect. These results suggest the possibility of pH sensitive microcapsules for high qualify of kimchi.

Effects of Vitamin E on Liver Cytochrome $P_{450}$ Content and Xanthine Oxidase Activity in Acute Cadmium-Poisoned Rats (식이 Vitamin E가 급성 카드뮴중독 흰쥐 간조직의 Cytochrome $P_{450}$ 함량과 Xanthine Oxidase 활성에 미치는 영향)

  • 김관유;채영미;이순재
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.6
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    • pp.1355-1363
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    • 1999
  • The purpose of this study was to investigate the effects of vitamin E on liver microsomal cytochrome P450 contents and xanthine oxidase activity in acute cadmium poisoned rats. Sprague Dawley male rats weighing 100$\pm$10g were randomly assigned to one normal and three cadmium injected groups. Cadmium injected groups were fed vitamin E free diet(0E Cd group), 40mg vitamin E per kg diet(40E Cd group) or 400mg vitamin E per kg diet(400E Cd group). Vitamin E level of normal group was 40mg per kg diet. Animals were injected intraperitoneally with 2.0mg Cd2+/kg bw for 4 days after the rats were fed diets with three different levels of vitamin E for 2 and 4 weeks. Body weight, food intake and feed efficiency ratio of cadmium injected animals, were decreased compared with those of normal group. The weights of liver and kidney in cadmium injected groups were not different from those of normal group. Cadmium contents of liver in cadmium groups were 160 fold higher those that of normal group. Accumulation of cadmium poisoned rat liver was reduced by vitamin E supplementation. Contents of blood hemoglobin and hematocrit in 0E Cd groups were decreased to 2~ 14% of those of the other groups. Contents of serum triglyceride in all experimental groups were not significantly different each other. Levels of serum total cholesterol, LDL cholesterol and antherogenic index in 0E Cd and 40E Cd groups were higher than those of normal group, while the contents of HDL cholesterol in 0E Cd and 40E Cd groups were lower than those of normal group. Xanthine oxidase(XOD) activity and cytochrome P450 contents in the liver were significantly increased in cadmium injected groups but these were reduced by vitamin E supplementations. The present results indicate that acute cadmium poisoning in rats causes increasing free radical generation systems in the liver and that leads to liver tissue damage. But these abnormalities can be reduced by dietary vitamin E supplementations.

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CHANGES IN ADHESION OF STREPTOCOCCUS MUTANS TO NANOCOMPOSITE RESINS AFTER ACIDULATED PHOSPHATE FLUORIDE GEL APPLICATION (나노컴포지트에서 Acidulated Phosphate Fluoride 적용에 따른 Streptococcus mutans 부착량 변화)

  • Jung, Young-Jung;Kim, Young-Jae;Kim, Jung-Wook;Jang, Ki-Taeg
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.3
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    • pp.427-436
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    • 2005
  • Topical application of APF gel can increase the surface roughness of resin composites and the roughened surfaces may allow increased bacterial accumulation and surface staining. Resin specimens of two proprietary resin composites, Filtek Z250(FZ) and Filtek Supreme Universal(FS), and experimental resin composites containing 0%, 3%, 6% nanofillers(E0, E3, E6) were fabricated and divided into two groups of the same number; APF treatment group and no treatment group. The amount of S. mutans adhered to specimens and the mean surface roughness(Ra) were measured. The results were as follows; 1. In no treatment group, the amount of S. mutans adhered to FS was the smallest. It was significantly different from those of FZ, E3, E6(p<0.05) although it was not significantly different from that of E0(p>0.05). 2. For all resin composites used, the amount of S. mutans adhesion in APF treatment group was significantly greater than that in no treatment group(p<0.05). 3. In APF treatment group, the amount of S. mutans adhesion was significantly different between materials(p<0.05), and increased in order of FS, FZ, E0, E3 and E6. 4. Difference of the surface roughness(Ra) between materials was not statistically significant in both no treatment group and APF treatment group(p>0.05)

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Genetic Polymorphism of Cytochrome P450 2E1 in Korean Population (한국인 집단에서 Cytochrome P450 2E1의 유전적 다형성)

  • 정혜광;구희경;이상섭;양규환;정태천;변부형
    • Environmental Mutagens and Carcinogens
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    • v.16 no.2
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    • pp.97-102
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    • 1996
  • In this study, we have quantified genotype frequency of the cytochrome P450 (P450) 2E1 which codes for the P450 enzyme primarily responsible for the metabolic activation of carcinogenic nitrosamines and low molecular organic solvents, in Korean population by using PCR and RFLP at two sites previously associated with some cancers; a PstI and RsaI RFLP in the transcriptional regulatory region of the human P450 2E1 gene. The genotype frequencies of homozygous wild type (PstI site-absent) and heterozygous mutant type (PstI site-present) in PstI RFLP were 0.70 and 030, respectively. The homozygous mutant type in Pstl RFLP was not observed in Korean population. The genotype frequencies of homozygous wild type (RsaI site-present), heterozygous mutant type (RsaI site-absent), and homozygous mutant type in RsaI RFLP were 0.71, 0.26, and 0.03, respectively.

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Structural Performance Evaluation of End-plate Connections According to Constructional Quality in P.E.B System (P.E.B 시스템에서 시공상태에 따른 엔드플레이트 접합부의 구조성능평가)

  • Lee, Eun-Taik
    • Journal of Korean Society of Steel Construction
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    • v.24 no.4
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    • pp.461-468
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    • 2012
  • P.E.B (Pre-Engineering Building) system means an economical system, which designs and uses optimal section proportion of tapered members according to the magnitude of bending moment. However, it is hard to adjust the friction type bolted joint in the joint of tapered member in the P.E.B system. End-plate connection is mainly used in this system due to that difficulty. Because P.E.B system has end-plate vertical defacts by heat welding deformation, a gap between end-plates and rafter or rib can be observed. In this study, an examination of construction stability was throughly performed and analyzed by the investigation of permissible internal force of bolts in end-plate connections under the bending moment using the end-plate's initial connection-defect (gap).

자원가격 불확실성 하에 북미 독립계 E&P기업의 투자옵션 연구

  • Gwon, O-Jeong;Park, Eun-Cheon;Park, Ho-Jeong
    • Environmental and Resource Economics Review
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    • v.21 no.3
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    • pp.441-464
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    • 2012
  • As prices of energy resources such as oil and gas started to rise steadily in 2000, energy security has been one of the most important topics in the world. To secure more energy, most of countries which are highly dependent on imported energy resources try to occupy oversea oil or gas reserves, thereby intensifying competition for energy resources around world. Under this circumstance, we focus on independent E&P companies since they are relatively small size companies which are suitable for M&A. We analyze investment option values for these E&P companies using a real option model for depletable resources. Based on analytical model, empirical study is provided to examine rationality of investment for energy companies. The result shows sufficient profitability for independent E&P companies in both oil and gas projects in the North America during 2004 to 2008. In Particular, oil projects were more feasible than gas project due to lower price of gas and high volatility of gas price at that time.

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Physiological Response of Barley to Water Stress and Salt Stress at Seedling Stage (보리 유묘기의 한해와 염해반응)

  • 최원열;박종환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.693-698
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    • 1997
  • Drought resistance and salt resistance of seedlings were compared with the polyethylene glycol(P.E.G) and NaCl solutions of the same osmotic potential($\Psi_\pi$=-10 bar). Plant height, seedling dry weight, chlorophyll content and leaf water potential decreased while the free proline content increased more in the P.E.G. than in the NaCl solution. Free amino acids increased 2.6 times in the P.E.G. solution and 3.6 times in the NaCl solution more than in the untreated(Hoagland's solution). Free proline occupied 66% and 61% of the content of total amino acids under water stress and salt stress, respectively. Besides free proline, phenylalanine in the P.E.G. solution and phenylalanine, alanine and asparagine in the NaCl solution increased distinctly. In short, it was shown that water and salt stress responses in seedling stage were relatively similar.

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Estimation on Chemical Water Quality Suitability Index for 4 Species of the Mayfly Genus Ephemera (Ephemeroptera: Ephemeridae) Using Probability Distribution Models (확률분포모형을 이용한 하루살이속(Ephemera) 4종에 대한 화학적 수질 적합도지수 평가)

  • Bongjun Jung;Dongsoo Kong
    • Journal of Korean Society on Water Environment
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    • v.39 no.6
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    • pp.475-490
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    • 2023
  • Chemical water quality suitability for species (Ephemera strigata, Ephemera separigata, and Ephemera orientalis-sachalinensis group) of the mayfly genus Ephemera (Order Ephemeroptera) was analyzed with probability distribution models (Exponential, Normal, Lognormal, Logistic, Weibull, Gamma, Beta, Gumbel). Data was collected from 23,957 sampling units of 6,664 sites in Korea from 2010 to 2021. E. orientalis-sachalinensis occurred at the range of BOD5 0.3~11.1 mg/L (the best-fit Lognormal model); T-P 0.007~0.769 mg/L (the Gumbel model); TSS 0.4~142.2 mg/L (the Lognormal model). E. strigata occurred at the range of BOD5 0.4~7.4 mg/L (the Gumbel model); T-P 0.007~0.254 mg/L (the Lognormal model); TSS 0.4~17.1 mg/L (the Lognormal model). E. separigata occurred at the range of BOD5 0.4~2.6 mg/L (the R-Weibull model); T-P 0.007~0.134 mg/L (the Lognormal model); TSS 0.7~10.0 mg/L (the Lognormal model). Habitat suitability range of E. orientalis-sachalinensis was estimated to be 0.4~1.9 mg/L (BOD5), 0.024~0.086 mg/L (T-P), 2.5~22.4 mg/L (TSS); that of E. strigata was 0.4~0.7 mg/L (BOD5), 0.007~0.018 mg/L (T-P), 0.0~1.7 mg/L (TSS); that of E. separigata was 0.0~0.4 mg/L (BOD5), 0.000~0.015 mg/L (T-P), 0.5~3.1 mg/L (TSS). In a relative comparision, E. orientalis-sachalinensis was estimated to be eurysaprobic, and narrowly adapted in high levels of T-P and TSS, E. strigata was estimated to be oligosaprobic and adapted in low levels of T-P and TSS, and E. separigata was estimated to be stenooligosaprobic and widely adapted in low level of T-P and TSS.