• Journal of Microbiology
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• 제43권3호
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• pp.301-307
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• 2005

The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, $Cu^{2+}$ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.

• 센서학회지
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• 제27권1호
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• pp.40-46
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• 2018

In this paper, we focus on optimization of a boron ($^{11}B$)-implanted n type Si epi substrate for obtaining near-zero temperature coefficient of resistance (TCR) at temperature range from 25 to $125^{\circ}C$. The $^{11}B$-implantation on the N type-Si epi substrate formed isolation from the rest of the N-type Si by the depletion region of a PN junction. The TCR increased as the temperature of rapid thermal anneal (RTA) was increased at the temperature range from $900^{\circ}C$ to $1000^{\circ}C$ for the $p^+$ contact with implantation at dose of $1E16/cm^2$, but sheet resistance of this film was decreased. After the optimization of anneal process condition, the TCR of $1126.7{\pm}30.3$ (ppm/K) was obtained for the $p^-$ resistor-COB package chips contained $p^+$ contact with the implantation of $5E14/cm^2$. This shows the potential of the $^{11}B$-implanted n type Si epi substrate as a resistor for pressure sensor in thermal stable environment applications..

• 한국전기전자재료학회논문지
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• 제33권3호
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• pp.173-176
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• 2020

This study focuses on a pillar in which is implanted a P-type maneuver under a P base. This structure is called a super junction structure. By inserting the pillar, the electric field concentrated on the P base is shared by the pillar, so the columns can be dispersed while maintaining a high breakdown voltage. Ten pillars were generated during the multi epitaxial process. The interval between pillars is varied to optimize the electric field to be concentrated on the pillar at a threshold voltage of 6 V, a yield voltage of 4,500 V, and an on-state voltage drop of 3.8 V. The density of the filler gradually decreased when the interval was extended by implanting a filler with the same density. The results confirmed that the size of the depletion layer between the filler and the N-epitaxy layer was reduced, and the current flowing along the N-epitaxy layer was increased. As the interval between the fillers decreased, the cost of the epitaxial process also decreased. However, it is possible to confirm the trade-off relationship that deteriorated the electrical characteristics and efficiency.

• 대한전자공학회논문지
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• 제18권4호
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• pp.49-61
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• 1981

N+P, N+PP+ 형 태양전지를 제조하여 얻은 실험자료들을 근거로 실리콘 접합형 태양전지에 일반적으로 적용할 수 있는 컴퓨터 모의 실험 프로그램을 개발하고, 이의 유용성을 확인하였다. 이 모의 실험 프로그램은 N+P, P+N, N+PP+, P+NN+형의 실리콘 태양전지에 적용할 수 있는 것으로, 입사광은 AMI, 등강도 광원, 인공조명으로 많이 사용되는 GE -ELH 광원이고, AR코팅은 Si3N4형과 투과도가 파장에 관계없는 일정형 2종류가 있으며, 프로그램에서 이들 전지의 구조, 광원, AR 코팅 종류에 대한 파장 특성분포도 쉽게 변화시킬 수 있도록 되어 있다. 이 모의실험의 결과들을 토대로 N+와 P 영역에서의 평균도오핑농도와 전지의 두께, AMI 스펙트럼에 대한 집광도, 앞면 접합깊이에 대하여 효율이 최대가 되는 최적치들을 구하였으며, 앞면의 표면 재결합 속도, 접합부에서의 캐리어의 유효 수명, 누설저항에 대하여는 허용 한계치로, 기타 효율변화인자로서 동작온도 직렬저항과 전기장의 세기에 대하여는 효율의 변화율로서 파라미터들이 효율에 미치는 영향들을 분석하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.506-514
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• 2020

Objective: We tested the hypothesis that increasing dietary copper (Cu) to gravid ewes would enhance brown adipose tissue (BAT) thermogenesis in their offspring. Methods: Twin-bearing ewes were assigned on d 70 of gestation to diets containing 3, 10, or 20 ppm dietary Cu (n = 8 per group). Twin lambs were assigned at birth to a cold (6℃) or warm (28℃) environmental chamber for 48 h. Blood was collected from ewes and from lambs and perirenal BAT was collected after 48 h in the environmental chambers. Results: Prenatal Cu exposure increased ewe plasma triiodothyronine (T₃) and thyroxine concentration (T₄) (p<0.01) but prenatal Cu exposure had no effect on lamb plasma concentrations of T₃, T₄, glucose, or nonesterified fatty acid concentration (p≥0.08). The high level of prenatal Cu exposure depressed 48-h rectal temperature (p = 0.03). Cold exposure decreased BAT norepinephrine (NE) and increased BAT dopamine (p≤0.01), but prenatal Cu exposure had no effect on BAT cytochrome C oxidase activity or BAT NE or dopamine (p≥0.07). However, BAT of lambs from high-Cu ewes maintained higher uncoupling protein-1 (UCP1) gene expression than BAT of lambs from low- and medium-Cu ewes following warm or cold exposure in environmental chambers (p = 0.02). Cold exposure caused near depletion of BAT lipid by 48 h (p<0.001), increased BAT cytochrome c oxidase activity (p<0.01), and depressed plasma fatty acid concentrations (p<0.001). Conclusion: Although prenatal Cu exposure increased BAT UCP1 expression during warm and cold exposure, prenatal cold Cu exposure depressed 48-h rectal temperature. Cold exposure decreased BAT lipid content by over 80% and decreased lamb plasma fatty acid concentration by over 40%, indicating that fuel reserves for thermogenesis were nearly depleted by 48 h of cold exposure.

• Journal of Korean Society for Atmospheric Environment
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• 제21권E1호
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• pp.23-35
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• 2005

Size distribution of particulate water-soluble ion components was measured at Gosan, Korea using a micro-orifice uniform deposit impactor (MOUDI). Sulfate, ammonium, and nitrate showed peaks in three size ranges; Sulfate and ammonium were of dominant species measured in the fine mode ($D_{p} < 1.8 {\mu}m$). One peak was observed in the condensation mode ($0.218\sim0.532{\mu}m$), and the other peak was obtained in the droplet mode ($0.532\sim1.8{\mu}m$). Considering the fact that the equivalent ratios of ammonium to sulfate ranged from 0.5 to 1.0 in these size ranges, it is inferred that they formed sufficiently neutralized compounds such as ($NH_{4})_{2}SO_{4} and (NH_{4})_{3}H(SO_{4})_{2}$ during the long-range transport of anthropogenic pollutants. On the other hand, nitrate was distributed mainly in the coarse mode ($3.1\sim6.2{\mu}m$) combined with soil and sea salt. Two sets of MOUDI samples were collected in each season. One sample was collected when the concentrations of criteria air pollutants were relatively high, but the other represented relatively clean air quality. The concentrations of sulfate and ammonium particles in droplet mode were the highest in winter and the lowest in summer. When the air quality was bad, the increase of nitrate was observed in the condensation mode ($0.218\sim0.282{\mu}m$). It thus suggests that the nitrate particles were produced through gas phase reaction of nitric acid with ammonia. Chloride depletion was remarkably high in summer due to the high temperature and relative humidity.

• The Korean Journal of Physiology and Pharmacology
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• 제9권6호
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• pp.315-322
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• 2005

In this study, the authors investigated whether death of vascular smooth muscle cell (VSMC) had a pathological pertinence. Conditioned media obtained from rat aorta smooth muscle cell (SMC) that were induced death by expressing FADD in the absence of tetracycline (FADD-SMC) triggered death of normal SMC. DNA fragmentation and caspase-3 activation were observed in dying SMC by conditioned media. FADD-SMC showed transcriptional activation of tumor necrosis factor $(TNF)-{\alpha}$. Conditioned medium contained $TNF-{\alpha}$, indicating secretion of the cytokine from dying FADD-SMC. It was investigated if secreted $TNF-{\alpha}$ was functional. Conditioned medium activated ERK and p38 MAPK pathways and induced MMP-9 expression, whereas depletion of the cytokine with its soluble receptor (sTNFR) remarkably inhibited induction of MMP-9 by conditioned medium. These findings suggest that $TNF-{\alpha}$ in conditioned medium seems to be active. Then, contribution of $TNF-{\alpha}$ on death-inducing activity of conditioned medium was examined. Depletion of $TNF-{\alpha}$ with soluble $TNF-{\alpha}$ receptor decreased the death activity of conditioned medium by 35%, suggesting that $TNF-{\alpha}$ play a partial role in the death activity. Boiling of medium almost completely abolished the death-inducing activity, suggesting that other heat labile death inducing proteins existed in conditioned medium. Taken together, these results indicate that SMC undergoing death could contribute to inflammation by expressing inflammatory cytokines and pathological complications by inducing death of neighboring cells.

• IMMUNE NETWORK
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• 제12권6호
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• pp.284-290
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• 2012

Innate immune cells sense and respond to the cytoplasmic infection of bacterial pathogens through NLRP3, NLRC4 or AIM2 inflammasome depending on the unique molecular pattern of invading pathogens. The infection of flagellin- or type III secretion system (T3SS)-containing Gram-negative bacteria such as Salmonella enterica serovar Typhimurium (S. typhimurium) or Pseudomonas aeruginosa (P. aeruginosa) triggers NLRC4-dependent caspase-1 activation leading to the secretion of proinflammatory cytokines such as interleukin-1-beta (IL-$1{\beta}$) and IL-18. Previous studies have shown that apoptosis-associated speck-like protein containing a CARD (ASC) is also required for Salmonella-induced caspase-1 activation, but it is still unclear how ASC contributes to the activation of NLRC4 inflammasome in response to S. typhimurium infection. In this study, we demonstrate that S. typhimurium triggers the formation of ASC oligomer in a potassium depletion-independent manner as determined by in vitro crosslinking and in situ fluorescence imaging. Remarkably, inhibition of potassium efflux failed to block Salmonella-promoted caspase-1 activation and macrophage cell death. These results collectively suggest that ASC is substantially oligomerized to facilitate the activation of caspase-1 in response to S. typhimurium infection. Contrary to NLRP3 inflammasome, intracellular potassium depletion is not critical for NLRC4 inflammasome signaling by S. typhimurium.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2012년도 학술발표회
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• pp.126-126
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• 2012

기후변화는 전 세계적으로 다양한 영향을 미치고 있으며 특히, 홍수나 대설로 인한 수문변화에 영향을 준다. 본 연구는 준분포형 연속 모형인 SWAT (Soil and Water Assessment Tool)모형을 이용하여 우리나라 3대 대설지역에 속하는 다목적댐인 충주댐유역(6642.0 m)의 기후변화에 따른 융설이 수문과 수질에 미치는 영향을 분석하고자 한다. 먼저, 융설 모형의 매개변수인 적설분포면적감소곡선 (Snow Cover Depletion Curve; SCDC)을 구축하기 위하여 10년(2000-2010)동안의 Terra MODIS (MODerate resolution Imaging Spectroradiometer) 위성영상자료와 6개 기상관측소(충주, 제천, 원주, 영월, 대관령, 태백)의 최심적설자료를 이용하여 연도별 SCDC을 구축하였다. 구축 결과, 눈이 50% 피복 일 때 snow volume은 연 평균 0.47로 분석되었다. 이를 SWAT 모형에 적용하여 수문과 수질에 대한 적용성 평가를 실시한 결과, 유출의 경우 NSE는 융설기간 동안 평균 0.8, 전체기간은 평균 0.6으로 나타났으며 수질(Sediment, T-N, T-P)의 경우 각각 평균 0.72, 0.70, 0.85을 나타내었다. 미래 기후자료는 IPCC(Intergovernmental Panel on Climate Change)에서 제공하는 SRES(Special Report on Emission Scenarios) A1B, B1 기후변화시나리오의 HadCM3 모델의 결과 값을 이용하였으며 기간은 과거 30년 기후자료(1981-2010, baseline)를 바탕으로 2040s(2020-2059), 2080s(2060-2099)의 두 기간으로 나누어 각각 분석하였으며 기후변화 결과 값의 불확실성을 줄이고자 과거 자료와 GCM의 1981년에서 2000년까지의 값을 비교하여 온도와 강수량의 보정을 실시한 후 LARS-WG를 이용하여 온도와 강수량 자료를 구축하였다. SWAT 모형을 적용한 결과, 평균 1.92 증가한 것으로 나타났으며 유출은 융설기간(Nov-Apr)이 비융설기간(May-Oct)보다 10% 더 증가하였다. 본 연구에서는 SWAT 모형을 통한 유출 및 환경부하량 전망을 목표로 하여 미래 기후변화를 고려한 융설이 다목적댐에서의 유출과 수질 (Sediment, Total Nitrogen, Total Phosphorus)에 미치는 영향을 평가해 보고자 한다.

• Journal of Korean Medical Science
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• 제33권50호
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• pp.299.1-299.13
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• 2018

Background: Sarcopenia is associated with a poor prognosis in patients with liver cirrhosis. However, it is not known whether the rate of skeletal muscle depletion is also associated with a poor prognosis. We investigated the prognostic impact of the rate of skeletal muscle depletion in patients with liver cirrhosis. Methods: We included retrospectively all patients with liver cirrhosis who underwent both multiple computed tomography scans and hepatic venous pressure gradient (HVPG) measurements. Results: A total of 131 patients with liver cirrhosis were enrolled. The mean age of the patients was 53.7 years and alcoholic liver disease was the most common cause (61.8%). Sixty-four patients (48.9%) were diagnosed with sarcopenia. The median changes in skeletal muscle area per year (${\Delta}SMA/y$) were -0.89%. During a median follow-up period of 46.2 months (range, 3.4-87.6), 45 patients (34.4%) died. In multivariate analyses, age, Child-Pugh score, HVPG, presence of sarcopenia and ${\Delta}SMA/y$ were independently associated with mortality. Cumulative mortality was significantly higher in patients with ${\Delta}SMA/y$ < -2.4% than those with ${\Delta}SMA/y{\geq}-2.4%$ (log-rank test, P < 0.001). Conclusion: Both the presence and rate of change of sarcopenia are independently associated with long-term mortality in patients with liver cirrhosis.