• 한국식품영양학회지
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• 제22권3호
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• pp.470-477
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• 2009

In this study, we evaluated the physicochemical and sensory quality of Glazed Spanish Mackerel in Teriyaki sauce containing added lemon juice and ginger juice using a Sous vide Cook-Chill method. The products were stored at below $3^{\circ}C$ and then its physicochemical and sensory quality was estimated after varous storage periods(0, 5, 10, 15 days). Measured extent of 40.91~52.04% in extent and reheating after of 42.35~54.40% when moisture content and did not significant by storage period in all sample are until save 15 days. The VBN was increased as the storage period increased. A similar trend was observed after reheating, and while lemon juice additions was significantly low on 15 days(p<0.01). Based on the results of the sensory evaluation, the Teriyaki sauces containing the added lemon juice additions were evaluated that had a better sensory quality is excellent in external appearance, flavor, taste, texture, and acceptability than the control group, and while were the evaluated excellently in overall flavor and taste in occasion of were found to be excellent after the addition of ginger juice.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1993년도 학술대회지
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• pp.138-142
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• 1993

Korean ginseng has been widely used as medicine from ancient times in Asia. Current breeding efforts in Korea include the individual plant selection and the subsequent pure - line isolation, and considerable number of lines with desirable traits have thus been isolated. However, there were rare data on genetic maker and its analysis for selection of superior varieties. For taxonomic characterization and development of genetic markers for ginseng breeding, molecular biological methods including the RFLP and RAPD methods were applied. Cytoplasmic DNA of ginseng was analyzed for RFLP analysis. However. there is no different pattern among the chloroplast DNA or mitochondrial DNA of variants. In the case of RAPD analysis, the band patterns using 4 of 10 RAPD primers show the distinctive polymorphism among 9 ginseng variants, and lines, and Similarity Index(SI) on polymorphism was calculated for the extent and nature of these variabilities in ginseng. The sequences of 4 selected primers were TGCCGAGCTG, AATCGGGCTG. GAAACGGGTG, and GTGACGTAGG. By SI based on the polymorphic band patterns, Chungkyung - Chong and Hwangskoog - Chong, and JakyungChong 81783 and Jinjakyung of Russia showed the most close SI. The data of KG10l coincided with the fact that it was released from Hwangskoog - Chong. and Jakyung - Chong 81783 and Jinjakyung of Russia showed the most close SI. The data of KG101 coincided with the fact that it was released from Hwangskoog - Chong by breeding process. The data of Jakyung strains indicated the significant variation among the strains. From these results, RAPD analysis method could be succesively applied to the classification and genetic analysis for breeding of Korean ginseng.

• Asian-Australasian Journal of Animal Sciences
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• 제29권12호
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• pp.1756-1760
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• 2016

Estimation of feed intake (FI) for individual animals within a pen is needed in situations where more than one animal share a feeder during feeding trials. A partitioning method (PM) was previously published as a model to estimate the individual FI (IFI). Briefly, the IFI of a pig within the pen was calculated by partitioning IFI into IFI for maintenance ($IFI_m$) and IFI for growth. In the PM, $IFI_m$ is determined based on the metabolic body weight (BW), which is calculated using the coefficient of 106 and exponent of 0.75. Two simulation studies were conducted to test the hypothesis that the use of different coefficients and exponents for metabolic BW to calculate $IFI_m$ improves the accuracy of the estimates of IFI for pigs, and that PM is applied to pigs fed in group-housing systems. The accuracy of prediction represented by difference between actual and estimated IFI was compared using PM, ratio (RM), or averaging method (AM). In simulation studies 1 and 2, the PM estimated IFI better than the AM and RM during most of the periods (p<0.05). The use of 0.60 as the exponent and the coefficient of 197 to calculate metabolic BW did not improve the accuracy of the IFI estimates in both simulation studies 1 and 2. The results imply that the use of $197kcal{\times}kg\;BW^{0.60}$ as metabolizable energy for maintenance in PM does not improve the accuracy of IFI estimations compared with the use of $106kcal{\times}kg\;BW^{0.75}$ and that the PM estimates the IFI of pigs with greater accuracy compared with the averaging or ratio methods in group-housing systems.

• Food Science and Biotechnology
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• 제14권6호
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• pp.732-737
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• 2005

The combinational effect of gamma irradiation and moist heating on the trypsin inhibitor activity (TIA) in soaked and dried soybeans was evaluated by measuring the inhibition using N-benzoyl-DL-arginine-p-nitroanilide (BAPNA) as substrate. Gamma irradiation significantly decreased the TIA level in soybean at doses above 5 kGy, and the $ID_{50}$ (the gamma irradiation dose required to reach 50% inhibition) value for TIA was 13.53 kGy. Soaking prior to gamma irradiation significantly lowered the $ID_{50}$ to 8.44 kGy, and the soaking process enhanced the efficiency to inactivate TIA by as much as 48%. When soaking prior to gamma irradiation was followed by subsequent mild heating ($60^{\circ}C$) process, the $IT_{50}$ (heating time required to reach the 50% inhibition of TIA) value at even 1 kGy (5.28 min) was greatly reduced by over 50% compared to the level for the no-soaking process. In addition, the activation energy of soaking prior to gamma irradiation at 1 kGy was 2.45 kcal/mole, which was also about 50% lower than the 5.10 kcal/mole of dried soybean gamma-irradiated. Based on these results, soaking prior to gamma irradiation is an effective method for TIA inhibition. Furthermore, a combination of two or more processing methods such as soaking, heating and gamma irradiation is much more effective than any single processing method.

• International Journal of Naval Architecture and Ocean Engineering
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• 제3권1호
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• pp.27-36
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• 2011

In the proposed paper numerical calculations are carried out using two versions of a three-dimensional, timedomain panel method developed by the group of Prof. P. Sclavounos at MIT, i.e. the linear code SWAN2, enabling optionally the use of the instantaneous non-linear Froude-Krylov and hydrostatic forces and the fully non-linear SWAN4. The analytical results are compared with experimental results for three hull forms with increasing geometrical complexity, the Series 60, a reefer vessel with stern bulb and a modern fast ROPAX hull form with hollow bottom in the stern region. The details of the geometrical modeling of the hull forms are discussed. In addition, since SWAN4 does not support transom sterns, only the two versions of SWAN2 were evaluated over experimental results for the parent hull form of the NTUA double-chine, wide-transom, high-speed monohull series. The effect of speed on the numerical predictions was investigated. It is concluded that both versions of SWAN2 the linear and the one with the non-linear Froude-Krylov and hydrostatic forces provide a more robust tool for prediction of the dynamic response of the vessels than the non-linear SWAN4 code. In general, their results are close to what was expected on the basis of experience. Furthermore, the use of the option of non-linear Froude-Krylov and hydrostatic forces is beneficial for the accuracy of the predictions. The content of the paper is based on the Diploma thesis of the second author, supervised by the first one and further refined by the third one.

• Geomechanics and Engineering
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• 제9권1호
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• pp.57-81
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• 2015

Water inrush and mud outburst always restricts the tunnel constructions in mountain area, which becomes a major geological barrier against the development of underground engineering. In view of the complex disaster-causing mechanism and difficult quantitative predictions of water inrush and mud outburst, several theoretical methods are adopted to realize dynamic assessment of water inrush in the progressive process of tunnel construction. Concerning both the geological condition and construction situation, eleven risk factors are quantitatively described and an assessment system is developed to evaluate the water inrush risk. In the static assessment, the weights of eight risk factors about the geological condition are determined using Analytic Hierarchy Process (AHP). Each factor is scored by experts and the synthesis scores are weighted. The risk level is ultimately determined based on the scoring outcome which is derived from the sum of products of weights and comprehensive scores. In the secondary assessment, the eight risk factors in static assessment and three factors about construction situation are quantitatively analyzed using fuzzy evaluation method. Subordinate levels and weight of factors are prepared and then used to calculate the comprehensive subordinate degree and risk level. In the dynamic assessment, the classical field of the eleven risk factors is normalized by using the extension evaluation method. From the input of the matter-element, weights of risk factors are determined and correlation analysis is carried out to determine the risk level. This system has been applied to the dynamic assessment of water inrush during construction of the Yuanliangshan tunnel of Yuhuai Railway. The assessment results are consistent with the actual excavation, which verifies the rationality and feasibility of the software. The developed system is believed capable to be back-up and applied for risk assessment of water inrush in the underground engineering construction.

• Wind and Structures
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• 제2권1호
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• pp.25-40
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• 1999

In this paper, artificial neural networks, a new kind of intelligent method, are employed to model and predict amplitude dependent damping in buildings based on our full-scale measurements of buildings. The modelling method and procedure using neural networks to model the damping are studied. Comparative analysis of different neural network models of damping, which includes multi-layer perception network (MLP), recurrent neural network, and general regression neural network (GRNN), is performed and discussed in detail. The performances of the models are evaluated and discussed by tests and predictions including self-test, "one-lag" prediction and "multi-lag" prediction of the damping values at high amplitude levels. The established models of damping are used to predict the damping in the following three ways : (1) the model is established by part of the data measured from one building and is used to predict the another part of damping values which are always difficult to obtain from field measurements : the values at the high amplitude level. (2) The model is established by the damping data measured from one building and is used to predict the variation curve of damping for another building. And (3) the model is established by the data measured from more than one buildings and is used to predict the variation curve of damping for another building. The prediction results are discussed.

• 디지털융복합연구
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• 제19권8호
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• pp.195-203
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• 2021

본 연구는 사람의 사용자 전신을 촬영한 이미지를 분석하여, 사용자별 움직임에 대한 정상 가동 범위를 영상으로 디스플레이(display)하는 운동 트레이닝 시스템 구축에 관한 것이다. 본 연구는 입점(立點, standing point)을 기준으로 사용자 전신의 정면과 측면을 촬영하는 카메라부(C), 그리고 카메라부의 촬영 이미지를 분석하여 인체의 관절 위치에 노드(node)를 설정하고, 입점을 원점으로 하는 공간좌표계에 노드를 배치하여 그 사용자의 노드 좌표값을 도출하는 이미지 분석부(I·A)와 이미 설정된 표준 노드 좌표값에 매칭된 운동 동작별 정상 노드 가동범위를 사용자 노드 좌표값에 맞추어 보정하여, 상기에서 언급한 사용자 노드 정상 가동 상태를 정면 영상과 측면 영상으로 생성하여 디스플레이하는 재생부(R·P)를 포함하고 있는 시스템으로 구성된다. 따라서 연구의 방법으로는 주요선행연구와 자료조사를 통해 선행 연구와의 차별성을 기할 수 있는 방법을 제시했으며, 이에 따른 운동 트레이닝 시스템을 구축하고자 하는데 연구의 목적을 둔 것이다.

• International Journal of Stem Cells
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• 제16권1호
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• pp.36-43
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• 2023

Background and Objectives: Lymphoblastoid cell lines (LCLs) deposited from disease-affected individuals could be a valuable donor cell source for generating disease-specific induced pluripotent stem cells (iPSCs). However, generation of iPSCs from the LCLs is still challenging, as yet no effective gene delivery strategy has been developed. Methods and Results: Here, we reveal an effective gene delivery method specifically for LCLs. We found that LCLs appear to be refractory toward retroviral and lentiviral transduction. Consequently, lentiviral and retroviral transduction of OCT4, SOX2, KFL4 and c-MYC into LCLs does not elicit iPSC colony formation. Interestingly, however we found that transfection of oriP/EBNA-1-based episomal vectors by electroporation is an efficient gene delivery system into LCLs, enabling iPSC generation from LCLs. These iPSCs expressed pluripotency makers (OCT4, NANOG, SSEA4, SALL4) and could form embryoid bodies. Conclusions: Our data show that electroporation is an effective gene delivery method with which LCLs can be efficiently reprogrammed into iPSCs.

• 한국식품저장유통학회지
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• 제20권4호
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• pp.564-572
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• 2013

본 연구에서는 전통 항아리를 이용하여 발효온도 및 여과 방법을 달리한 현미식초를 제조하여 이들의 품질특성을 조사하였다. 초산발효에 사용하는 발효제와 온도에 따라 적정산도는 발효 4~5주에 가장 높았으며, 강제여과가 전통여과방식보다 산도가 더 높게 나타났다. pH는 모든 구간에서 발효가 진행됨에 따라 감소하였고, 당도는 각각의 발효액 모두 초산 생성이 원활할 때는 발효초기와 비슷하게 유지되었지만, 발효액이 과산화 되었을 때는 당도가 낮아졌다. 유기산 함량은 발효온도와 여과방식에는 큰 차이는 없지만 다양한 발효제에 따라 발효 초기에 8종(oxalic, citric, malic, tartaric, succinic, lactic, acetic 및 formic acid 등)이 검출되었고, 발효가 진행되면서 acetic acid가 많이 생성되었다. 총 유리아미노산 함량은 초산 함량이 증가할수록 유지되거나 감소의 폭이 적었으며, 초산함량이 감소하는 발효 6주에 총 아미노산 함량도 급격히 감소하는 것으로 나타났다. 항아리에서 초산발효가 진행됨에 따라 초산함량이 증가할 때 유리 아미노산 함량은 감소하였다. 따라서 본 연구에서는 발효온도 및 여과방식을 달리함에 따라 유기산 및 유리 아미노산 함량의 차이가 큰 것으로 판단되며, 초산 발효액의 경우, 향후 숙성과정에서 유기산 및 유리 아미노산의 변화가 있을 것으로 예상되므로 장기 숙성에 따른 초산 발효액의 품질변화에 대한 연구가 요구된다.