• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제2권1호
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• pp.57-68
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• 1998

Polycyclic aromatic hydrocarbons (PAMs) are ubiquitous contaminants in marine environments. PAMs enter estuarine and nearshore marine environment via several routes such as combustion of fossil fuels, domestic and industrial effluents and oil spills. PAHs have been the focus of numerous studies in the world because they are potentially carcinogenic, mutagenic, and teratogenic to aquatic organisms and humans from consuming contaminated food. However, one can hardly find any available data on PAM content in marine organisms in Korea. The present study was carried out in order to determine PAM content in oysters from the intertidal and subtidal zones of Chinhae Bay, which is located in near urban communities and an industrial complex, and the bay is considered to be a major repositories of PAHs. 16 PAHs were analyzed by High Performance Liquid Chromatography (HPLC) with uv/vis and fluorescence detectors in oysters: they are naphthalene (NPTHL), acenaphthylene (ANCPL), acenaphthylene (ACNPN), fluorene (FLURN), phenanthrene (PHEN), anthracene (ANTHR), fluoranthene (FLRTH), pyrene (PYR), benzo(a)anthracene (BaA), chrysene (CHRY), benzo(b)fluoranthene (BbF), benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), dibenz(a,h)anthracene(DahA), benzo(g,h,i)peryne (BghiP) and indeno(1,2,3,-cd)pyrene (I123cdP). The PAM contents in oysters from the intertidal and subtidal zones of Chinhae Bay ranged from < 0.1 to 992.0 ug/kg (mean $69.8\pm9.8$ ug/kg).

• 한국지반공학회논문집
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• 제19권6호
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• pp.7-14
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• 2003

본 연구에서는 굴패각 혼합토의 전단강도 특성을 파악하고자 해성토에 굴패각 혼합율 0%, 25% 그리고 50%로 달리 한 후 $K_o$ 비배수 삼축압축 실험을 실시하였다. 또한 각 혼합비에 따라 유효 연직압을 200kPa, 300kPa 그리고 400kPa로 적용하였고, 유효 연직압 300kPa에 대해서 전단속도를 세가지로 달리하여 그에 따른 혼합토의 전단강도 특성을 알아보았다. 일련의 실험결과로부터 굴패각 혼합비가 증가할수록 굴패각 입자의 파쇄로 인해 전단강도가 증가함을 볼 수 있었으며, 전단속도는 굴패각 혼합토의 전단강도에 큰 영향을 미치지 못함을 확인하였다. 굴패각 혼합토의 실내실험과 Mayne, Bishop의 경험식을 토대로 비교 분석한 결과 비배수 전단강도는 실험값과 비슷한 경향을 나타내고 있다. 그러나 간극수압계수 예측은 혼합비 0%에서 경험식이 실험값과 보다 과대 평가를 보이고 있다.

• 한국수산과학회지
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• 제50권2호
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• pp.120-129
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• 2017

This study assessed the safety of raw oysters Crassostrea gigas for consumption during processing in a processing plant. Bacterial contamination (e.g., viable cell counts, coliform groups, Escherichia. coli and pathogenic bacteria) and chemical contamination (e.g., heavy metals and shellfish toxins) were measured on raw oysters, a processing equipment, employees and work areas. No total mercury, lead, paralytic shellfish poison, diarrheic shellfish poison or norovirus was detected in any post-harvested oyster samples. However, the cadmium level ranged from 0.1-0.2 mg/kg. The viable cell count, E. coli and coliform group levels in post-harvested oysters ranged from 4.00-4.54 log CFU/g, ND-210 MPN/100 g and 110-410 MPN/100 g, respectively. The viable contaminating cell counts on employees, equipment and work areas were in the range of $0.90-3.46log\;CFU/100cm^2$. Airborne bacteria in the work areas ranged from 0.60 to 1.81 log CFU/plate/15 min. Thus, no significant health risks were detected in the processing plant.

• 한국수산과학회지
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• 제49권5호
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• pp.533-540
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• 2016

This study assessed the risk of an oyster-shucking site to establish the hazard analysis critical control point (HACCP) system model by measuring viable cell counts, coliform group Staphylococcus aureus foreign material on oysters, oyster-producing equipment, and washing water. The viable cell count and coliform group levels of the harvested raw oysters were 4.00 log CFU/g and 1.1×10² MPN/100 g, while those of washed oysters were 2.99 log CFU/g and (3.2−4.6) × 10 MPN/100 g, respectively. After washing the oysters, no Escherichia coli or pathogenic bacteria (E. coli O157:H7, Listeria monocytogenes, S. aureus, Salmonella spp., Vibrio parahaemolyticus, and Clostridium perfringens) were detected. Regardless of the location of foreign matter, up to 100% more metallic and non-metallic foreign matter was detected at 1.5 mmΦ than at 3.5 mmΦ, using a metal detector with increased sensitivity. According to the results, the critical control points (CCP) are the washing and metal-detection processes. These results can be used as basic data to improve sanitation at oyster-shucking sites in factories with an HACCP system.

• 한국수산과학회지
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• 제51권2호
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• pp.121-126
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• 2018

The purpose of this study was to assess bacteriological hazards in the following 7 kinds of minimally processed shellfish products purchased from Korean seafood retail outlets: raw oysters stored with seawater in polyethylene bags, frozen oysters, raw mussels, frozen mussels, boiled mussels, raw short-necked clam, and frozen short-necked clams, obtained from Korean seafood retail outlets. The total coliform and Escherichia coli counts determined in all samples were detected below regulation limit of the Korean government guidelines (Food Code). In addition, the high-risk bacterial pathogens, Listeria monocytogenes, Salmonella spp., and E. coli O157:H7 were not detected in any samples. Low-risk pathogens such as Staphylococcus aureus and Vibrio parahaemolyticus were detected at levels above $1.0{\times}10^2$ colony forming unit (CFU)/g in some minimally processed shellfish products. Notably, S. aureus was detected in all samples. Raw oysters stored with seawater in polyethylene bags, frozen oysters, and boiled blue mussels are commonly ingested without heating, and therefore these minimally processed shellfish products pose bacteriological hazards. The detection of S. aureus in all shellfish products, an organism indicative of poor personal hygiene, which can grow and multiply during distribution, indicates the necessity of improving the food safety of minimally processed shellfish products.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.159-164
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• 2013

The mouse bioassay and high performance liquid chromatography (HPLC) post-column oxidation method are different methods of quantifying paralytic shellfish poisoning toxins. In this study, we compared their ability to accurately quantify the toxicity levels in two types of field sample (oysters and mussels) with different toxin profiles for routine regulatory monitoring. A total of 72 samples were analyzed by both methods, 44 of which gave negative results, with readings under the limit of detection of the mouse bioassay ($40{\mu}g/100g$ saxitoxin [STX] eq). In 14 oysters, the major toxin components were gonyautoxin (GTX) 1, -2, -3, -4, -5, decarbamoylgonyautoxin-2 (dcGTX2), and decarbamoylsaxitoxin (dcSTX), while 14 mussels tested positive for dcSTX, GTX2, -3, -4, -5, dcGTX2, neosaxitoxin (NEO), STX, and dcSTX. When the results obtained by both methods were compared in two matrices, a better correlation ($r^2=0.9478$) was obtained for mussels than for oysters ($r^2=0.8244$). Additional studies are therefore needed in oysters to investigate the differences in the results obtained by both methods. Importantly, some samples with toxin levels around the legal limit gave inconsistent results using HPLC-based techniques, which could have a strong economic impact due to enforced harvest area closure. It should therefore be determined if all paralytic shellfish poisoning toxins can be quantified accurately by HPLC, and if the uncertainties of the method lead to doubts regarding regulatory limits.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.137-142
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• 2013

To understand critical aspects of paralytic shellfish poisoning (PSP) in a chief area of bivalve production in Korea, seasonal variation in PSP toxins in bivalves collected from Jinhae Bay, Korea in 2009 was surveyed by the pre-column high-performance liquid chromatography oxidation method. We also confirmed the profiles of major bivalves such as oysters Crassostrea gigas and mussels Mytilus galloprovincialis in Jinhae Bay. PSP toxins in the bivalves showed remarkable seasonal variation. PSP toxin levels were detected from April to May in 2009, and the highest total toxin levels at all stations were recorded in May. The major toxins in bivalves were gonyautoxin [GTX] 1&4 and C 1&2; in oysters GTX 2&3 were also detected as major components. GTX 1&4, which showed the highest PSP toxin levels at each station, accounted for the highest proportions of toxin components in mussels and oysters (64.5-71.3% and 41.4-42.4%, respectively). It was also confirmed that the highest toxicity (in ${\mu}g$ saxitoxin [STX] eq/g) was derived from GTX 1&4. The highest total toxicity (in ${\mu}g$ STX eq/g) was approximately 2-8-fold higher in mussels than in oysters collected from the same station. PSP toxin levels in bivalves differed significantly according to the sample collection station. However, the profiles of toxins in the bivalves did not show significant differences during the survey period according to sample collection station. This study shows that PSP toxin levels in some samples from Jinhae Bay were above the regulatory limit in Korea during a specific period in spring.

• 한국수산과학회지
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• 제35권4호
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• pp.327-335
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• 2002

Effects of available food on growth of commercially cultured Pacific oysters, Crassostrea gigas in Goseong Bay on the south coast were studied using a numerical model. levels of total protein, carbohydrate and lipid in particulate organic matter in the water column as well as chlorophyll a concentration were determined for estimating total available food for oyster growth. Environmental parameters including water temperature, salinity and total suspended solid were also monitored for the model. Oyster growth was also monitored by means of measuring shell length and tissue wet weight increase on a monthly basis. Simulation results from the numerical model indicated that chlorophyll a is not a good representative of available food for the oysters in Goseong Bay. In contrast, available food in the water column measured by filtration of the organic particles and analyzed in terms of total lipids, carbohydrates and protein was well matched with simulated oyster growth in the Bay which is similar to observed growth. The model also suggested that oysters have relatively low retention efficiency of $50\%$ or less. This result indicates that oysters in the bay utilize only a part of food particle available in the water column, as reported in other studies.

• 한국환경과학회지
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• 제2권1호
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• pp.57-68
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• 1993

Polycyclic aromatic hydrocarbons (PAMs) are ubiquitous contaminants in marine environments. PAHs enter estuarine and nearshore marine environment via several routes such as combustion of fossil fuels, domestic and industrial effluents and oil spills PAHs have been the focus of numerous studies in the world because they owe potentially carcinogenic, mutagenic, and teratogenic to aquatic organisms and humans from consuming contaminated food. However, one can hardly find any available data on PAM content in marine organisms in Korea. The present study was carried out in order to determine PAH content in oysters from the intertidal and subtidal zones of Chinhae Bay, which is located in near urban communities and an industrial complex, and the bay is considered to be a major repositories of PAHs. 16 PAHs were analyzed by High Performance Liquid Chromatography (HPLC) with uv/vis and fluorescence detectors in oysters: they are naphthalene (NPTHL), acenaphthylene (ANCPL), acenaphthene (ACNPN), fluorene (FLURN), phenanthrene (PKEN), anthracene (ANTHR), fluoranthene (FLRTH), pyrene (PYRf), benzo(a)anthracene (BaA), chrysene (CHRY), benzo(b)- fluoranthene (BbF), benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), dibenz(a, h)anthracene (DhA), benzo(g, h, i)peryne (Bghip) and indeno(1, 2, 3, -cd)pyrene (I123cdP). The PAH contents in oysters from the intertidal and subtidal zones of Chinhae Bay ranged from < 0.1 to 992.0 $\mu\textrm{g}$/kg (mean 69.8 $\pm$ 9.8 $\mu\textrm{g}$/kg). Key words . polycyclic aromatic hydrocarbon, high performance liquid chromatography, oyster, Chinhae Bay.

• 농업생명과학연구
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• 제46권6호
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• pp.105-115
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• 2012

국내산 양식 및 천연산 굴의 가치 증진 및 보다 효율적인 활용을 위한 식품학적 자료를 얻기 위해 통영, 고성, 여수 및 사천만에서 수하식 및 반수하식으로 양식한 참굴 4종과 고흥, 서천의 해안가 조간대에 서식하는 천연산 참굴류 2종을 구입하여 이들의 영양성분과 풍미성분 조성을 분석 비교하였다. 탈각한 양식산 및 천연산 굴의 크기는 각각 길이 4.7~5.1 및 3.0~4.2 cm, 무게 5.9~9.1 및 2.6~5.5 g으로 산지에 따라 약간 차이가 있었으나, 일반성분 조성의 차이는 거의 없었다. 아미노질소 및 휘발성염기질소 함량은 각각 232.8~258.2 및 160.5~213.9 mg/100 g, 9.5~12.0 및 7.8~9.5 mg/100 g으로 산지에 따라 약간의 차이를 나타내어 맛과 냄새의 강도가 다소 차이가 있음을 확인하였다. 염도는 각각 1.5~1.7 및 1.5~1.8%, 절단력은 95~114 및 105~132 g으로 산지에 따른 차이는 거의 없었다. 양식산과 천연산 굴의 주요 구성지방산은 16:0, 18:0, 16:1n-9, 18:1n-9, 22:1n-9, 16:4n-3, 20:5n-3, 22:6n-3 등으로 산지에 따른 특별한 조성의 차이는 없었다. 총아미노산의 함량은 각각 9,004~10,198 및 8,165~8,942 mg/100 g으로 aspartic acid, glutamic acid, proline, alanine, leucine, phenylalanine, lysine 및 arginine이 주요 구성 아미노산이었고, 주요 무기이온은 K, Na, Ca, Fe, S, P 및 Zn 등으로 산지에 따라 차이를 보였다. 정미발현성분인 유리아미노산의 총함량은 각각 1,444~1,620 및 1,017~1,277 mg/100 g으로 taurine, glutamic acid, glycine, alanine, tryptophan, ornithin 및 lysine 등이 주요 유리아미노산이었으며, 산지에 따라 다소간의 차이를 나타내었다.