• 한국식품저장유통학회지
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• 제12권5호
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• pp.482-488
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• 2005

산화적인 스트레스(Oxidative stress)는 신경염증의 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 약용곤충추출물을 이용하여 항산화 물질을 찾고자 초고속 적용가능한 스크리닝 방법을 적용하였다. 우선, 분자염증은 활성산소 관련의 물질과 밀접한 관련이 있으므로 이들의 억제를 동반하는 추출물을 먼저 선별하였다. 항산화 실험과 관련하여 DPPH (1,1-Diphenyl-2-picrylhydrazyl), FRAP(Ferric ion reducing antioxidant power), HO (Hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 assay하였고 hydrogen peroxide(H2O2)에 의한 세포사멸 억제 활성을 보기 위해 MTT assay를 실시하였다. 실험 결과, 사마귀, 늦반딧불이, 무당벌레에서 다른 library에 비교하여 항산화 활성이 높게 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.541-549
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• 2015

Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer (BC) with higher metastatic rate and both local and systemic recurrence compared to non-TNBC. The generation of reactive oxygen species (ROS) secondary to oxidative stress is associated with DNA damage, chromosomal degradation and alterations of both hypermethylation and hypomethylation of DNA. This study concerns differential methylation of promoter regions in specific groups of genes in TNBC and non-TNBC Saudi females in an effort to understand whether epigenetic events might be involved in breast carcinogenesis, and whether they might be used as markers for Saudi BCs. Methylation of glutathione S-transferase P1 (GSTP1), T-cadherin (CDH13), Paired box protein 5 (PAX5), death associated protein kinase (DAPK), twist-related protein (TWIST), DNA-binding protein inhibitor (ID4), High In Normal-1 (HIN-1), cyclin-dependent kinase inhibitor 2A (p16), cyclin D2 and retinoic acid receptor-${\beta}$ ($RAR{\beta}1$) genes was analyzed by methylation specific polymerase chain reaction (MSP) in 200 archival formalin-fixed paraffin embedded BC tissues divided into 3 groups; benign breast tissues (20), TNBC (80) and non-TNBC (100). The relationships between methylation status, and clinical and pathological characteristics of patients and tumors were assessed. Higher frequencies of GSTP1, ID4, TWIST, DAPK, PAX5 and HIN-1 hypermethylation were found in TNBC than in non-TNBC. Hypermethylation of GSTP1, CDH13, ID4, DAPK, HIN-1 and PAX5 increased with tumor grade increasing. Other statistically significant correlations were identified with studied genes. Data from this study suggest that increased hypermethylation of GSTP1, ID4, TWIST, DAPK, PAX5 and HIN-1 genes in TNBC than in non-TNBC can act as useful biomarker for BCs in the Saudi population. The higher frequency of specific hypermethylated genes paralleling tumor grade, size and lymph node involvement suggests contributions to breast cancer initiation and progression.

• 생명과학회지
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• 제19권3호
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• pp.343-348
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• 2009

벼도열병균은 벼의 주요 병해인 벼도열병의 원인균이다. 식물병원균의 침입 시 식물체로부터 발생하는 ROS는 식물의 방어기작으로 중요하며, 특히 아미노산의 하나인 methionine은 ROS에 의해 산화되어 methionine sulfoxide로 변화될 수 있다. 식물병원균은 식물체로 부터의 ROS에 의한 산화반응을 회피하기 위해 methionine sulfoxide reductase B (MSRB)와 같은 항산화 효소를 가지는데 본 연구에서는 벼도열병균에서의 MSRB 유전자를 동정하고 분자적 특성을 살펴보았다. MSRB 유전자는 벼도열병균의 게놈 상에 단일 유전자로 존재하며 과산화수소 처리에 의해 유전자발현이 다소 증가하는 경향을 보였다. 이러한 결과로 MSRB 유전자는 벼도열병균의 항산화 기작에 관여할 가능성이 높다고 판단된다.

• 생명과학회지
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• 제30권8호
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• pp.722-730
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• 2020

대기오염은 더 이상 무시할 수 없는 심각한 건강 위험요소 중 하나로 여겨지고 있다. 미세먼지는 각종 생화학적 오염물질, 유기 화합물, 무기 화합물 및 중금속 등의 유해성분을 포함한 대기오염의 주요 구성 미립자 성분이다. 최근 여러 연구에서 미세먼지가 암, 심혈관질환, 호흡기질환 및 피부질환 등을 이환율과 관련이 있다고 밝혀져 있다. 그러나 눈이 대기 오염에 직접 노출되는 기관 중 하나임에도 불구하고, 미세먼지에 의한 눈의 영향 평가는 소수에 그치고 있다. 본 총설에서는 역학 및 임상 결과, 생체 내 및 생체 외 실험 결과를 바탕으로, 미세먼지 노출과 안구표면질환, 망막질환 및 녹내장 등의 안구질환의 발달 사이의 연관성을 제시하였다. 또한 미세먼지 노출이 산화적 스트레스, 염증반응, 자가포식 등을 통해 안구 표면질환을 야기할 수 있다는 사실을 제시하였다. 그러나 현재까지 미세먼지에 대한 대부분의 안구 영향 평가가 안구 표면질환에 국한되어 이루어지고 있으며, 후안부의 병리학적 발생 기전은 아직 밝혀져 있지 않다. 따라서, 미세먼지 노출에 대한 안구 영향 평가는 안구 표면을 포함하여 후안부에 대한 지속적인 역학, 임상 및 기초 연구가 수반되어야 할 것으로 사료된다.

• 한국식품과학회지
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• 제52권1호
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• pp.60-66
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• 2020

본 연구는 DCE의 항산화 활성 및 신경세포 보호 효과에 평가하였다. 감 심지로부터 유용성 성분을 얻기 위하여 에탄올 추출을 한 결과 약 10.36±1.34%의 추출물의 수율을 얻을 수 있었다. DCE의 총 폴리페놀, 총 폴라보노이드 함량과 항산화 활성으로 DPPH, ABTS 라디칼 소거능 및 환원력을 평가한 결과, DCE의 항산화 활성이 농도 의존적으로 증가하는 것으로 나타났다. 또한, DCE의 HT22 세포 보호 효과에 관하여 알아보기 위하여 HT22 세포에 DCE을 처리한 후 H₂O₂를 통한 산화적인 스트레스의 유도를 통하여 세포독성에 미치는 영향을 확인한 결과, DCE의 처리는 HT22 세포에 독성이 나타나지 않았으며, 이에 따라 항산화 효소인 SOD 활성이 증가와 지질과산화 생성물인 MDA level의 감소를 확인 할 수 있었다. 이러한 결과들로 볼 때, DCE의 항산화 및 산화적 스트레스로부터 신경세포 보호효과를 확인함으로서 향후 퇴행성 신경질환 예방에 유용한 건강기능성 식품 소재로서의 개발 가능성이 높을 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5145-5151
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• 2013

Background: Numerous carcinogens and reactive oxygen species (ROS) may cause DNA damage including oxidative base lesions that lead to risk of nasopharyngeal carcinoma. Genetic susceptibility has been reported to play a key role in the development of this disease. The base excision repair (BER) pathway can effectively remove oxidative lesions, maintaining genomic stability and normal expression, with X-ray repair crosscomplementing1 (XRCC1), 8-oxoguanine glycosylase-1 (OGG1) and apurinic/apyimidinic endonuclease 1 (APE1) playing important roles. Aims: To analyze polymorphisms of DNA BER genes (OOG1, XRCC1 and APE1) and explore their associations, and the combined effects of these variants, with risk of nasopharyngeal carcinoma. Materials and Methods: We detected SNPs of XRCC1 (Arg399Gln), OGG1 (Ser326Cys), APE1 (Asp148Glu and -141T/G) using the polymerase chain reaction (PCR) with peripheral blood samples from 231 patients with NPC and 300 healthy people, furtherly analyzing their relations with the risk of NPC in multivariate logistic regression models. Results: After adjustment for sex and age, individuals with the XRCC1 399Gln/Gln (OR=1.96; 95%CI:1.02-3.78; p=0.04) and Arg/Gln (OR=1.87; 95%CI:1.29-2.71; p=0.001) genotype variants demonstrated a significantly increased risk of nasopharyngeal carcinoma compared with those having the wild-type Arg/Arg genotype. APE1-141G/G was associated with a significantly reduced risk of NPC (OR=0.40;95%CI:0.18-0.89) in the smoking group. The OR calculated for the combination of XRCC1 399Gln and APE1 148Gln, two homozygous variants, was significantly additive for all cases (OR=2.09; 95% CI: 1.27-3.47; p=0.004). Conclusion: This is the first study to focus on the association between DNA base-excision repair genes (XRCC1, OGG1 and APE1) polymorphism and NPC risk. The XRCC1 Arg399Gln variant genotype is associated with an increased risk of NPC. APE1-141G/G may decrease risk of NPC in current smokers. The combined effects of polymorphisms within BER genes of XRCC1 399Gln and APE1 148Gln may contribute to a high risk of nasopharyngeal carcinoma.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.856-863
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• 2013

Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

• Toxicological Research
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• 제34권3호
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• pp.231-239
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• 2018

Bisphenol A, an everywhere chemical, is applied as a plasticizer in polycarbonate plastics, which often used in our everyday products and in epoxy resins as protective coatings and linings for food and beverage cans for decades. Human exposure to BPA may lead to adverse effects by interfering with oestrogen receptors. Our present study was conducted to investigate the protective effects of selenium (Se) and vitamin E (Vit E) on BPA-induced damage in the liver of male rats. Animals were randomly divided into four groups: the first group received olive oil and served as control. The second group received both (Se + Vit E) (0.5 mg/kg diet; 100 mg/kg of diet). The third one treated orally by (10 mg/kg b.w.) of BPA. The last group received (Se + Vit E) (0.5 mg/kg diet; 100 mg/kg of diet) concomitantly with (10 mg/kg b.w.) BPA. Exposure to BPA for three weeks engendered a hepatic disorder. An increased AST and ALT enzymatic activity was noticed in BPA-treated group as compared to other groups. Furthermore, a change in glucose, cholesterol, LDL-C, HDL-C, albumin, and bilirubin level was remarkable. Moreover, exposure to BPA increased malondialdehyde levels while reduced gluthatione content was decreased in the liver homogenate. A decrease in glutathione peroxidase, glutathione s-transferase and catalase activities was observed in the same group. Administration of selenium and vitamin E through the diet in BPA treated rats ameliorated the biochemical parameters cited above. In addition, an improvement in activities of liver enzymes was recorded. The histological findings confirmed the biochemical results. The model of this study that we employed characterized the relationships between BPA-induced hepatotoxicity and its alleviation by natural antioxidants like selenium and vitamin E.

• 한국동물위생학회지
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• 제36권4호
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• pp.233-242
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• 2013

The genus Taraxacum, known by the common name Dandelion, is a medicinal herb in the family Asteraceae. It has been traditionally used as a folk medicine for the treatment or prevention of various diseases due to its anti-inflammatory and anti-oxidative properties. In this study, we attempted to evaluate protective effects of Dandelion related with anti-oxidative activity to Thioacetamide (TAA)-induced liver damage. 36 rats were randomly assigned to six experimental groups : Control, Dandelion water extract (DWE), TAA, TAA&DWE 300, TAA&DWE 600, TAA&DWE 1,200 groups. Rats in DWE and TAA&DWE groups were pretreated with DWE (300, 600 or 1,200 mg/kg BW) by gavage for 7 days. All rats were treated intraperitoneally with TAA (200 mg/kg BW) or normal saline at 12 hours after last oral administration and sacrificed at 12 hours after last treatment. Levels of WBC and Neutrophil count were significantly decresed in TAA&DWE 1,200 group compared with that in TAA group (P<0.05). In TAA&DWE 600 and TAA&DWE 1,200 groups, serum AST, ALT, GGT levels were lower than TAA group (P<0.05). The serum TG level was significantly elevated in TAA&DWE groups compared with those in TAA group. Liver tissues from TAA group showed extensive histopathological changes, characterized by moderate or severe hepatocytes degeneration, inflammatory cell infiltration, and congestion. In the TAA&DWE group, The severity of histopathological lesions were decreased compared to those in the TAA group. The MDA concentration was significantly decreased and GSH content was significantly increased in the TAA&DWE 1,200 group compared to those in the TAA group. GR, CAT and GST activities in the TAA&DWE 1,200 group were significantly increased compared to those in the TAA group.

• 한국초지조사료학회지
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• 제29권4호
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• pp.299-306
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• 2009

저온 스트레스에 대한 내성을 지닌 신품종 톨 페스큐를 개발할 목적으로 CaMV35S 프로모터 하류에 NDP kinase 2 유전자가 항상적으로 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 나타내며 재분화된 형질전환 식물체를 pot로 이식하여 기내순화 시킨 후, Southern blot 분석을 실시하여 본 결과, NDP kinase 2 유전자가 형질전환 식물체의 genome에 정상적으로 도입되었음을 확인하였다. 프로테옴 분석을 통하여 도입유전자의 조절을 받는 항산화관련 유전자들의 발현이 유도되었음을 확인 할 수 있었다. 형질전환 식물체 잎 절편을 산화스트레스 중의 하나인 저온 스트레스를 처리하여 세포의 손상 정도를 조사한 결과, 비형질전환체에 비해 형질전환체는 강한 내성을 나타내었다. 또한 유식물체 수준에서 저온 스트레스를 처리하여 내성을 비교한 결과, 비형질전환체에 비해 형질전환체는 높은 내성을 나타내었다. 이 형질전환 톨페스큐는 산화스트레스 내성 품종개발을 위한 소재로 활용 될 수 있을 것이다.