• Natural Product Sciences
• /
• 제16권2호
• /
• pp.133-139
• /
• 2010

In order to develop a new skin whitening agent, approximately 100 plant extracts were evaluated for their inhibitory activities against melanin biosynthesis in cultured mouse melanocyte melan-a cells. As a result, seven extracts exhibited over 50% inhibition of melanin synthesis compared to control at a concentration of $20\;{\mu}g/ml$. In particular, Aster ageratoides Turcz. var. ageratoides (branch, root, aerial, flower; $IC_{50}$ = 17.3, 6.1, 13.6, $12.9\;{\mu}g/ml$, respectively) and Physalis alkekengi var. francheti (leaf, unripen fructus, aerial; $IC_{50}$ = 6.5, 28.3, $23.9\;{\mu}g/ml$) markedly inhibited melanin synthesis. In addition, tyrosinase activity was monitored by the measurement of dopachrome formation from the oxidation of L-3,4-dihydroxyphenylalanine. Extracts of A. ageratoides Turcz. var. ageratoides (flower) and P. alkekengi var. francheti (leaf) showed the most potent tyrosinase inhibitory activity. These plants might be the potential candidate sources in the development of novel skin-whitening products.

• 동아시아식생활학회지
• /
• 제23권4호
• /
• pp.423-429
• /
• 2013

In this study, to develop a new anti-aging agent, we will examine the antioxidant activity and the inhibitory effects of the mosidae for the MMP-1 activities in UVB-irradiated HS68 human foreskin fibroblast cell lines and human keratinocytes (Ha- CaT cell line). And the consomme soup being prepared with mosidae according to different levels (3, 6, 9, 12%) and then quality characteristics will be determined by sensory evaluations and color values. It is also observed that the mosidae has the inhibition of UVB-induced MMP-1 activities. The consomme soup prepared with mosidae is found to reduce the level of $IC_{50}$ in SOD-like activities and hydroxy radical scavenging activities, respectively. And consomme soup prepared with mosidae (CS6) is the best by sensory evaluations. In conclusion, these results suggest that the consomme soup prepared with mosidae can be further developed as new anti-aging food.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제21권2호
• /
• pp.169-174
• /
• 2010

Hydrolysate silk fibroin (HSF) is a fibrous protein composed of parallel $\beta$-structures and is made from pure silk elements including 18 amino acids, with glycine, alanine, and serine comprising of over 80% of the amino acids. Numerous studies have documented a range of effects of HSF, including moisturizing, antioxidant activity, nervous system disorders, and many more. We investigated whether HSF has anti-obesity effects in vitro. The effects of HSF inhibition on lipid accumulation and acceleration of lipid degradation in 3T3-L1 cells were studied. Treatment of 3T3-L1 cells with HSF caused significant inhibition of cell viability, an increase in glycerol release, and a decreased in adipocyte differentiation. Moreover HSF stimulated downregulated of adipogenic enzyme expressions (PPAR${\gamma}$ and C/EBP${\alpha}$) and up-regulated of fatty oxidation enzyme expressions (CPT-1 and UCP-2). Based on these results, hydrolysate silk fibroin can be suggested as a potential therapeutic substance as part of a prevention or treatment strategy for obesity.

• 한국산림과학회지
• /
• 제99권6호
• /
• pp.836-842
• /
• 2010

본 연구는 기능성 식 약용 소재로 널리 이용되는 헛개나무의 화장품원료로의 개발을 위하여 다양한 생리활성을 측정하였다. 헛개나무 과병과 뿌리의 열수추출물 및 에탄올추출물에 대한 DPPH법, ABTS법을 이용하여 항산화활성 측정시 열매, 뿌리 모두 열수추출물에서 뛰어난 항산화효과를 나타냈으며, 멜라닌 색소합성에 관여하는 tyrosinase의 활성을 측정한 결과 tyrosinase 저해활성은 과병에탄올추출물(HDFE) 500 mg/L에서 32.0%의 억제능을 확인 할 수 있었고, 뿌리에탄올추출물(HDRE)은 21.8%의 억제능을 보였다. 더 높은 농도인 1,000 mg/L에서는 과병열수추출물(HDFW), HDFE 순으로 저해활성이 나타났다. 주름개선효과에 관한 활성은 collagenase와 elastase 저해활성을 통해 측정하였다. 그 결과 elastase에서 과병에탄올 추출물을 제외한 모든 추출물이 두 효소를 모두 저해하는 것으로 나타났으며, 같은 농도에서는 collagenase의 활성이 더 크게 저해됨을 보였다. 이와 같은 결과를 통해 헛개나무추출물의 항산화활성과 미백 및 주름개선효과를 이용하여 기능성 화장품으로 활용이 가능할 것으로 사료된다.

• Journal of Microbiology
• /
• 제45권6호
• /
• pp.578-582
• /
• 2007

The primary objective of this study was to assess the in vitro melanogenesis inhibitory effects of methanolic extracts of the edible and medicinal lichens, Umbilicaria (Gyrophora) esculenta and Usnea longissima. The quantities of the total phenolic compounds of methanolic extract of the two lichen extracts were determined to be 1.46% and 2.62%, respectively. In order to evaluate the antioxidative effects of the extracts, we also measured electron donating abilities (EDA) and lipid peroxidation rates. The EDA values measured by the reduction of 1.1'-diphenyl-2-picrylhydrazyl (DPPH) were 72.8% and 80.7% for the extracts, with $SC_{50}$ (median scavenging concentration) values of $1.29{\pm}0.05\;mg/ml$ and $1.03{\pm}0.06\;mg/ml$, respectively. The rates of inhibition of lipid peroxidation using linoleic acid were 92.1% and 97.3% for the extracts, with $IC_{50}$ (median inhibitory concentration) values of $0.57{\pm}0.05\;mg/ml$ and $0.53{\pm}0.06\;mg/ml$, respectively. The inhibitory rates of the extracts against tyrosinase were 67.4% and 84.8%, respectively. The extracts were shown to reduce melanin formation in human melanoma cells. Melanin contents in the samples treated with 0.01% and 0.1% U. esculenta were 47.1% and 31.2%, respectively, and those treated with 0.01% and 0.1% Usnea longissima were 51.1% and 34.9%, respectively, whereas a value of 54.0% was registered when ascorbic acid was utilized as a positive control. In addition to direct tyrosinase inhibition, it was determined that the lichen extracts affected the activity of tyrosinase via the inhibition of tyrosinase glycosylation. As a result, the methanolic extracts of U. esculenta and Usnea longissima evidenced melanogenesis inhibitory effects, which occurred via multiple routes.

• KSBB Journal
• /
• 제16권1호
• /
• pp.54-60
• /
• 2001

저품위 광석으로부터 유가 금속을 회수하기 위한 생물용출법의 적용에 있어 미생물의 활성은 매우 중요하며, 제련미생물은 금속이온에 대해 어느 정도 내성을 가지고 있어야 한다. 본 연구에서는 대표적인 제련미생물인 Thiobacillus ferrooxidans의 철산화속도에 미치는 단독 혹은 혼합 금속이온의 영향을 조사하여, T. ferrooxidans의 금속이온에 대한 내성 특성을 조사하였다. 생장 배지에 $Zn^{2+}$, $Cu^{2+}$, $Ni^{2+}$, 및 $Cd^{2+}$를 단독으로 첨가한 경우(첨가농도범위, $Zn^{2+}$ 60g/L이하, $Cu^{2+}$, $Ni^{2+}$, 및 $Cd^{2+}$ 6 g/L 이하)에는 T. ferrooxidans에 의한 철산화속도는 금속이온의 첨가량에 크게 저해 받지 않았다. $Zn^{2+}$를 제외한 $Cu^{2+}$, $Ni^{2+}$, 및 $Cd^{2+}$의 2 성분 혹은 3 성분의 혼합금속이온을 첨가한 경우에는 T. ferrooxidans의 철산화 활성에 대한 혼합 금속이온의 저해 효과는 최대 50% 정도로 크게 나타나지는 않았다. 그러나, $Zn^{2+}$을 타 금속이온과 2 성분 혹은 3 성분을 혼합하여 생장 배지에 첨가한 경우에는 T. ferrooxidans의 철산화 활성 저해에 대한 혼합 금속이온의 상승 효과가 큰 것을 밝혔다.

• 한국자원식물학회지
• /
• 제24권2호
• /
• pp.236-242
• /
• 2011

제주산 녹차 및 발효 정도를 20%, 50%, 80%로 달리한 3종류의 발효차를 $90^{\circ}C$ 열수에 추출하여 추출물의 항산화능, 지질과산화 억제능 그리고 혈관내피세포(CPAE)를 이용하여 산화스트레스에 대한 세포 보호효과를 조사하였다. 녹차나 다른 발효차에 비해 20% 발효차에서 항산화능을 평가하는 DPPH 소거능이 가장 우수한 것으로 나타났다. 20% 발효차의 80 ${\mu}g/mL$ 처리 시 50 ${\mu}M$ 농도의 EGCG 처리와 거의 유사한 free radical 소거율을 보였다. 녹차와 3종류의 발효차 모두 LDL 산화를 억제하여 우수한 지질과 산화 억제능을 보였으나 특히 저농도인 40 ${\mu}g/mL$ 처리 시에 녹차와 20% 발효차의 경우 다른 발효차에 비해 25~30% 정도 더 LDL의 산화가 억제 되어 50 ${\mu}M$/mL EGCG 처리 시와 거의 동일한 수준의 지질과산화 억제 효과를 보였다. CPAE 세포에 녹차 및 3종류의 발효차 추출물을 처리한 실험에서 전혀 추출물을 처리하지 않은 세포에 비해 세포활성이 40~60% 증가하였다. 특히 세포에 1 mM 과산화수소를 처리하여 산화스트레스를 유발한 실험에서 녹차 및 발효차 추출물의 처리가 세포의 산화스트레스에 대한 보호효과를 나타내는 것을 알 수 있었다. 이상의 결과들은 녹차 및 발효차 추출물이 세포 내 free radical을 제거함으로써 산화스트레스에 대한 세포 보호효과에 중요한 기능 중의 하나로 작용한 것과 20% 발효차가 다른 발효차에 비해 우수한 항산화기능을 나타낸다는 것을 보여주었다.

• 대한본초학회지
• /
• 제30권5호
• /
• pp.51-58
• /
• 2015

Objectives : This paper aimed to verify the applicability of mixed extract ofAngelica gigasNakai,Cnidium officinaleMakino,Paeoniala ctifloraPall,Rechmannia glutinosaLibosch,Scutellaria baicalensisGeorgi, which were prescribed for improving inflammation in Donguibogam, as the materials for beauty food and functional medicinal herb cosmetics by manufacturing such mixed extract and evaluating the biological activity of the extract.Methods : The mixed medicinal herb water extract(MMW) and ethanol extract(MME) were freeze-dried to be used as the specimen. We performed electron donating ability, lipid acidification inhibitory activity, anti-inflammatory activity against skin flora, MTT assay, NO inhibitory activity and the protein expression inhibitory activity of iNOS and COX-2.Results : For anti-oxidation experimentation, the electron donating abilities of MMW and MME were above 60.0% and 90.0% at 500 μg/ml, respectively. In the inhibition rate of lipid peroxidation, MMW and MME showed 43.1% and 52.1% at 1,000 μg/ml, respectively. As a result of antimicrobial activity, both the MMW and MME showed significant clear zones forPropionibacterium acnesat 4 mg/disc, but did not indicated the clearzones forStaphylococcus aureus, Escherichia coliandStaphylococcus epidermidis. Anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited in a concentration-dependent manner. Also, the expression of iNOS and COX-2 proteins were significantly inhibited following treatment with MMW and MME of 50 μg/ml.Conclusions : Accordingly, it can be concluded that mixed medicinal herb extract has the potential to beused as a functional food and cosmetic material.

• Journal of Ginseng Research
• /
• 제4권2호
• /
• pp.133-145
• /
• 1980

In order to find out the inhibitors of acetic acid fermentation in Korean ginseng (Panax Sin son C. A. Meyer), total aglycone, panaxadiol, panaxadiol, oleanolic acid and ${\beta}$ -sitosterol were added to the basal medium, respectively, and a surface culture was carried out at 30$^{\circ}C$. The results were as follows: 1 . Saponins lost their activity to inhibit the acetic acid fermentation by hydrolysis. 2 Panaxadiol inhibited slightly, and the degree of inhibition was about 1/300 of that of free saponins. 3. Panaxadiol and oleanolic acid inhibited silighly similar to total aglycone. 4. Acetic acid fermentation was stimulated at the early stage when ${\beta}$-sitosterol was added to the media below the level of 0.000815%. But the fermentation was inhibited when media contained it more than that media 5. An over-oxidation of acetic acid was observed when the media contained total aglycone. panaxadiol, panaxatriol, oleanolic acid and ${\beta}$-sitosterol, respectively, while the media which contained sucrose, ginseng extracts ginseng saponins was shown not to be over-oxidized.

• KSBB Journal
• /
• 제13권3호
• /
• pp.279-283
• /
• 1998

To investigate the feasibility of the microbial process for removal of heavy metals from the high solid content sludge, the effect of sludge concentration on the solubilization of heavy metals by an iron oxidizing bacterium Thiolbacillus ferrooxidans was examined. With increasing the sludge concentration, the removal efficiency of heavy metals and the oxidation rate of iron were inhibited. Especially, when the sludge concentration is over 5% (w/v), the activity of T. ferrooxidans was remarkably inhibited. This inhibition is considered to occur due to the dissolved inhibitory materials such as organic compounds, heavy metals, and others which were extracted from the sludge during incubation period. In conclusion, the microbial process by T. ferrooxidans is only effectively used in ranges of 1.3 to 4.0% (w/v) sludge concentration.