• Korean Journal of Microbiology
• /
• v.52 no.1
• /
• pp.120-124
• /
• 2016

A certain size of DNA (28-29 kb long) to be packaged into P2-size head and the mutation in sid gene of bacteriophage P4 are the major factors to overcome "P2 sir-associated helper inefficiency". To clarify whether the presence of sid mutation is essential to overcome "P2 sir-associated helper inefficiency" or not, we tested the P4 derivative, P4 delRI::kmr, which is $sid^+$ and whose genome size supposed to be 28.5 kb long in the case of being packaged into $P2_{sir3}$-sized large head. As P4 delRI::kmr showed the low EOP with P2 sir3 lysogen, P4 delRI::kmr phage stock was prepared in P2 sir3 lysogen host to increase the EOP with P2 sir3 lysogen. Through this process, P4 delRI::kmr had been adapted for P2 sir3 lysogen. With a CsCl buoyant equilibrium density gradient experiment and gel electrophoresis of the isolated DNA, it was evident that the adaptation of P4 delRI::kmr for P2 sir3 lysogen was caused by the conformational change of DNA to be packaged into large head. The burst size determination experiments with P4 delRI::kmr phage stock adapted for P2 sir3 lysogen and normal P4 delRI::kmr phage stock showed that not the sid mutation but the size of DNA to be packaged (28-29 kb long) was essential to overcome "P2 sir-associated helper inefficiency".

• Ocean and Polar Research
• /
• v.33 no.4
• /
• pp.473-483
• /
• 2011

In the recent WTO/DDA negotiations, discussions are progressing to forbid fishery subsidies which have negative effect on the natural environment and the fishery resources and to lower or to eliminate the tariff/non-tariff barriers that distort trade liberalization. As a result, linking to the weak structural environment of Korea's fishing villages; such a scheme has jeopardized the livelihood of fishermen who settle down in local fishing harbors. Against this backdrop, the government is attempting to positively respond to changes in the fishing environment in a number of different ways: promotion of fishing tourism, fishing harbor reinforcement in function, developing fishing villages, and harbors. With respect to investment by priority in fishing harbor development, it is very important to appropriately select investment targets based on objective criteria. A small number of harbors are chosen and investment plans by harbor are systematically devised, economic feasibility and the effects of investment are analyzed and reviewed in relation to each target harbor. This paper is designed to review and assess objective evaluation methods and the degree of importance for the designation of target harbors, dealing with local harbors in Chung Nam Do as a model. Each local government has tried to reorganize existing harbors and develop new harbors, considering a fall in the number of fishing vessels and the expansion of maritime leisure activities. In order to overcome the limit of existing harbors in function, to raise the income of fishermen, and to activate tourism, they have shifted their focus from simple functions such as vessel evacuation and anchoring to harbor beautification, which can work as a basis for fishing tourism. This paper points out that the AHP analysis method for prioritizing local harbors in Chung Nam Do should help to be chosen target harbors in other cities and provinces. Each local government has attempted to reorganize existing harbors and to develop new harbors, taking into consideration the decline in the number of fishing vessels and the expansion of maritime leisure activities. In order to overcome the limited number of functional harbors, to raise the income of fishermen, and to boost tourism, they have shifted their focus from simple functions, such as vessel evacuation and anchoring, to harbor beautification, which can work as a basis for fishing tourism. This paper shows that the AHP analysis method for prioritizing local harbors in Chungcheongnam-do could aid in the effective selection of target harbors in other cities and provinces.

• Korean Journal of Clinical Laboratory Science
• /
• v.47 no.4
• /
• pp.298-305
• /
• 2015

Stem cell-like tumor cells are reported to be the main reason for tumor recurrence and metastasis. As one of the new approaches to overcome cancer, studies are emerging to inhibit the expressions of stem cell transcriptional factors (Oct4, Sox2, Klf-4, and Lin28) in cancer cells. MicroRNAs are master genetic regulators that can control development and differentiation of stem cells. In this study using various ovarian tumors (Skov3, Ovcar3, Tov112D, Tov21G, PA-1 and Hsc832(c)T), we examined the expressions of stem cell-related transcription factors, and the biological changes in cell survival and growth by miR-126 that targets stem cell transcriptional factors. We observed that treatment of miR-126 induced the morphological changes and cell suspension in most cells. In addition, miR-126 induced gradual regression of cell division except Skov3 cells, especially significant time-dependent reduction in Tov112D, Tov21G and PA-1. When we examined the expression of stem cell transcriptional factors, Sox2 was shown to be down-regulated after miR-126. Our results demonstrate that miR-126 treatment can provide the reversible environment to regulate cell division and to induce cell death of ovarian tumors, suggesting the molecular biological clues for clinical usage.

• Korean Journal of Construction Engineering and Management
• /
• v.18 no.6
• /
• pp.3-12
• /
• 2017

In recent years, Project Management Consultant(PMC) project has been emerging as a high-value-added engineering project. However, domestic engineering firms have difficulties in entering the overseas PMC market because of poor project performance and lack of PMC capabilities. To overcome these limitations, this study analyzes the key competitiveness factors for successfully performing the global PMC projects. PMC competency factors based on Porter's double diamond theory were extracted, and a questionnaire survey was conducted on domestic and overseas engineering firms. In addition, the important PMC competency factors were derived through paired t-test and IPA matrix analysis. As a result, the PMC competitiveness of domestic engineering firms was analyzed as around 60% compared to that of global engineering firms. Furthermore, 'PMC track records' and 'project management/owner response skills' were identified as top priority competency factors which are the most necessary factors for present domestic engineering firms. This study is expected to support to establish the international PMC market entry strategies by providing priorities of key competitiveness factors.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.8
• /
• pp.207-215
• /
• 2019

This study was conducted to identify key success factors of the Agricultural Cooperative Federation. We classified the divisions of regional agricultural cooperatives into high performers and low performers to investigate differences in environmental stability, resource capacity, resource flexibility, differentiation strategy, and low-cost strategies between high and low performers. Empirical analyses revealed that high performers are superior to low performers in resource capacity, resource flexibility, differentiation, and low-cost, but there was no difference in environmental stability between high performers and low performers. Internal factors including resource and strategy attributes affect business performance, but external factors such as environmental attributes do not. Moreover, resource flexibility generates the broadest gap between high performers and low performers. These results indicate that resource flexibility is the key factor leading to success of organizations with greater competitors because it enables them to overcome their disadvantageous competitive position in size and resources through complete exploitation of resources across divergent business areas owing to its flexibility. Our contribution is that we draw the key success factors of the Agricultural Cooperative Federation through an empirical approach.

• Journal of the Korean Regional Science Association
• /
• v.34 no.4
• /
• pp.3-18
• /
• 2018

These days, regional innovation draws more attention than ever as a growth engine for regional economies, and governments put a variety of efforts to establish Regional Innovation systems(RISs). In this circumstance, this study aims to analyze types of RISs and the combinations of the factors influencing innovation performance as measured by patent application. Most of previous works have depended on case-oriented or variable-oriented strategy to classify types of RISs or to analyze the effects on performance of innovation factors, having some limitations: Variable-oriented approaches fail to capture complex combinatory effects of factors, while case-oriented approaches tend to depend on subjective interpretation. This study made use of the recently proposed fs/QCA(Fuzzy-set Qualitative Comparative Analysis) to overcome the limitations of those strategies. Based on the theory of RIS, three factors for regional innovation-input, infrastructure, and network-are used to classify 16 Korean Provinces. The results show that eight types of regional innovation types are identified, and that most of the regions are classified into either IN-type, equipped with high levels of Input and Network, or F-type, with high levels of infrastructure. In addition, applying seven sub-variables of the three factors to the fussy-set combination factor analysis, we examine a combination of factors influencing patent application. The results show that regions with high levels of R&D expense, valid patent, industry-academia cooperation, IP budget, and TLO values, and low IP capital almost always have a high level of patent application. Therefore, for regional innovation, the public sector needs to provide institutional support for R & D personnel training. It is also important to for both the public and the private sectors to make efforts to stimulate IP financing.

• The Journal of the Korea Contents Association
• /
• v.22 no.1
• /
• pp.274-282
• /
• 2022

In order to secure pedestrians' right to walk, we have up-cycled second hand mobile phones to overcome limitations of the existing survey methods, analysis methods, and diagnosis to reduce pedestrian traffic accidents. Second hand mobile phones were up-cycled to produce mobile CCTVs and installed in areas where pedestrian deaths rate is high to secure image data sets for the period of more than 24 hours. It was analyzed by applying image visualization technology and clouding reporting technology, and more precise and accurate results were derived through modeling based on artificial intelligence learning and GIS-based diagnostic guidance. As a result, it was possible to analyze the risk factors and number of pedestrian safety, and even factors that were not known in the existing method could be derived. In addition, the traffic accident risk index was derived by converting data into one year to verify whether second hand mobile phone up-cycling mobile CCTV will be an objective tool for finding pedestrian risk factors. Up-cycling mobile CCTV of second hand mobile phones newly applied through research can be used as a new tool to find pedestrian risk factors, and it can be used as a service to protect the safety of the traffic vulnerable other than pedestrians.

• Korean Journal of Culture and Social Issue
• /
• v.13 no.2
• /
• pp.1-15
• /
• 2007

The purpose of this study is to find how the factors of "Grief of loss" affect on the subjective well-being of elderly('the satisfaction with life', 'the positive affect', 'the negative affect). One hundred of subjects, over age 65, participated in the survey and we could find that the "Grief of loss" was composed of four sub-factors('economical loss', 'loss from being parted by death', 'loss of physical functions', 'loss of relations'). These four sub-factors of "Grief of loss" showed negative correlations with the scores of the satisfaction with life. However, three other sub-factors except 'loss from being parted by death' showed negative correlations with the scores of 'the positive affect'. On the other hand, other three sub-factors except 'economical loss' showed positive correlations with the scores of 'the negative affect'. Especially, the 'economical loss' was affecting on 'the negative affect' and 'the positive affect' significantly. Also, 'loss of relations' was affecting on both 'the negative affect' and 'the satisfaction with life''. Two out of the four sub-factors, 'economical loss' and 'loss from being parted by death', showed no significant effects on the subjective well-being. This results insists us that the emotional supports, especially from their offspring, are truly important for elderly to overcome their Grief of loss('loss of physical functions', 'loss of relations')

• Journal of The Korean Association For Science Education
• /
• v.33 no.4
• /
• pp.763-777
• /
• 2013

This is a case study on two schools practising integrated science education (hereafter ISE). The purposes of this study are to investigate the types and features of ISE in the schools actively practising ISE, to identify the contextual factors of the schools, and to give implications for implementing ISE in schools. This study investigated the contextual factors in practicing ISE with a focus on the two schools, a middle school in Gyeonggi-do and a high school in Busan. They were breaking down the boundaries among teaching subjects and providing student-oriented instruction with problems in the real world. The data were collected by observing classes, by interviewing teachers, and by reviewing school documents and students' reports. The research findings are as follows: first, the two schools took part in ISE actively. They teach science to students providing integrated experiences mainly by using interdisciplinary knowledge and/or by solving the problems pertaining to the real world. While the former integrated subjects centering on topics, the latter focused on a project-based learning driven by students. They have differences in regard to the role of teachers and students, the level of integration and the type of integration. Second, the contextual factors that enabled ISE to be implemented there were found. The previous studies revealed six contextual factors in practising ISE: small and stable learning environment, leadership, team activities, in-school planning time, flexible timetable and community links. This study also found similar factors. However, the cases of this study provided ISE on a large scale and in a short period of time, instead of a small and stable learning environment. Teachers viewed the process of ISE not only as a tool to overcome the conservative culture of teachers but also as a pursuit of innovation.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.8 no.3
• /
• pp.97-111
• /
• 2013

This study reviews preceding research for detailed factors to establish development strategies for Korean Hidden Champion firms and classifies strategy factors into internal ones and external ones through specialists' opinions to draw strengths, weaknesses, opportunities and threats of each factor. It also sets hierarchical model to draw up a survey, distributes the survey to groups of specialists and enterprises respectively and then examines consistency ratio. Fifty-four copies of survey whose reliability on responses is secured through examining the consistency ratio are evaluated with their relative importance in factors by using SWOT/AHP technique and their order of priority is determined. Based on their results, development strategies for Korean Hidden Champion firms are established. SWOT/AHP analyses results show that external factors are with the opportunity of industry growth and the threat of intensified competition and market uncertainty and internal factors are with the strength in order of technological competence, construction competence in customer relation and marketing competence. The weakness in the lack of funds, lack of brand awareness in order. This result suggests that external environments of enterprises that more emphasis should be put on the industry growth and aggressive strategies cannot help but be adopted even in a global competition getting fiercer every day are seen more important. Then, it also seems to be thought that the technological competence including R&D and specialization, construction competence in customer relation and marketing competence should internally chosen for strategies to support strategies. The order of priority in development strategies for Korean Hidden Champion firms is drawn as; (i) aggressive S/O strategy which utilizes opportunities by taking advantage of strengths, (ii) W/O strategy which utilizes opportunities by supplementing weaknesses, (iii) diversified S/T strategy which utilizes strengths to make up for threats and (iv) defensive W/T strategy which supplements weaknesses to overcome threats.